pycom13g23740

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0017374
Physical Location & Seq
Forward (+)
21150409 .. 21151854
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g23740.8

Sequence Viewer

Length: 1002 bp
ATGCATAATAGCGGCCTTTGGAGTGACTTCCACAAACACCTTCATGGATTGAACTTAGATGATTTAGGTCGTCTGGAGGCGCTAGGCAGCGGGGATGTGGTACACGTGCTGCCTTTGCTTGAAGCTTCCCAGACTTCAATCTGCCCAAGCGGATTTGGAGTTGCTGGCTTCACCTCTACGCTTGGCTTGTCTAGATACTCCACCAGAATAAAGCGTTTGAGTTGGTGGTCGAGGGTGGAACCTAAGTCGGCTAGCGCGTTCGCATGGCCATTGTTTGCCCTTGCTTTCGTACCTTTTCTAGTTACTGCACCATCTTCAGATGTTTTTTCTATAAGATTTTTCACTGCCAAGTCTTTTGCCATTCGGAGGCCTGCTAGTAGGGCTTCGTACTCTGCTTTGTTGTTGGATGCTTTGAAGCCTAGAATGATTACCTACTCGAGCATCGAACCATCTGGGGTGACAAGGACCATGCCTAAGTCTTCATTTGGTGAAGTAGATGTGGCTAAGACGGGCTCGGTTGCTAGGTGTGAAGGCGCGGTAGAGAGCCGTGGAACTGAGGCCATGTTACACGCAAAAGGAGTTGCTCAACTTTTGGGTCGTGTGACACGTAACAGGAGGTCGTGCTCAACTGCAGGTACATGTTGCTTCTATTTAAAATCTTTTGGTGTGATAAGGATAAAACTTGCTTTCGAGTGTGTCCTTCTAGTGTTCTTTTGCCCTTGGCGTGCCTTTTGGGCTGAGTTGTTGTGTTTGTCAAAAAACTTTGCATCCGCCAGTGTTTACTCCTTTATCGTTGCGTATAACTTGAGCCTCCGGGTTACAACAACTATCACCAAAGATACTTTTGAATTGTGGGATGCAGGTAGTCTGGCCCCCTGCTTTTCTCATATGAGGGTAGAACTTATTGCTGCTTCATATATTGTCAAGCATGTAAATAAGTCTTCCGTTGCTTCTGGTTTGGATAATAGGGAGGTGATGTCGGGTACTTCTTCAAGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

334

Amino Acids

36.42

Weight (kDa)

9.5

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 623, 851
AccII CGCG 2 cut(s) 257, 536
AciI CCGC 5 cut(s) 12, 90, 150, 536, 771
AcoI YGGCCR 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 300
AcvI CACGTG 1 cut(s) 106
AfaI GTAC 5 cut(s) 102, 291, 389, 637, 985
AfiI CCNNNNNNNGG 1 cut(s) 366
AflIII ACRYGT 3 cut(s) 103, 605, 638
AgsI TTSAA 6 cut(s) 52, 122, 138, 415, 848, 993
AluBI AGCT 1 cut(s) 125
AluI AGCT 1 cut(s) 125
Alw21I GWGCWC 1 cut(s) 626
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 5 cut(s) 13, 266, 368, 558, 870
ApeKI GCWGC 3 cut(s) 87, 109, 908
AspLEI GCGC 3 cut(s) 82, 257, 536
AspS9I GGNCC 2 cut(s) 465, 871
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 5 cut(s) 163, 469, 500, 823, 985
AsuNHI GCTAGC 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 1 cut(s) 465
BalI TGGCCA 1 cut(s) 268
BanII GRGCYC 1 cut(s) 515
BbrPI CACGTG 1 cut(s) 106
BbsI GAAGAC 2 cut(s) 471, 933
Bbv12I GWGCWC 1 cut(s) 626
BbvI GCAGC 3 cut(s) 96, 99, 895
BccI CCATC 2 cut(s) 319, 457
BceAI ACGGC 1 cut(s) 531
BcnI CCSGG 1 cut(s) 815
BfaI CTAG 8 cut(s) 83, 192, 252, 299, 375, 420, 522, 704
BfmI CTRYAG 1 cut(s) 630
BfoI RGCGCY 1 cut(s) 83
BfuAI ACCTGC 2 cut(s) 623, 851
BglI GCCNNNNNGGC 1 cut(s) 734
BisI GCNGC 4 cut(s) 13, 88, 110, 909
BlsI GCNGC 4 cut(s) 14, 89, 111, 910
Bme1390I CCNGG 1 cut(s) 815
Bme18I GGWCC 1 cut(s) 465
BmeT110I CYCGRG 1 cut(s) 436
BmgT120I GGNCC 2 cut(s) 465, 871
BmiI GGNNCC 2 cut(s) 240, 873
BmrFI CCNGG 1 cut(s) 815
BmsI GCATC 4 cut(s) 397, 450, 776, 847
BmtI GCTAGC 1 cut(s) 255
BpiI GAAGAC 2 cut(s) 471, 933
BpmI CTGGAG 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 826
BpuMI CCSGG 1 cut(s) 815
BsaAI YACGTR 2 cut(s) 106, 608
BsaJI CCNNGG 2 cut(s) 547, 719
BsaXI ACNNNNNCTCC 2 cut(s) 962, 992
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 774
BseDI CCNNGG 2 cut(s) 547, 719
BseGI GGATG 4 cut(s) 100, 412, 767, 862
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 546, 729
BseNI ACTGG 1 cut(s) 774
BseXI GCAGC 3 cut(s) 96, 99, 895
BsgI GTGCAG 1 cut(s) 291
Bsh1236I CGCG 2 cut(s) 257, 536
BshFI GGCC 5 cut(s) 15, 268, 370, 560, 872
BsiHKAI GWGCWC 1 cut(s) 626
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 1 cut(s) 814
BslI CCNNNNNNNGG 1 cut(s) 366
BsnI GGCC 5 cut(s) 15, 268, 370, 560, 872
BsoBI CYCGRG 1 cut(s) 436
Bsp1286I GDGCHC 2 cut(s) 515, 626
BspACI CCGC 5 cut(s) 12, 90, 150, 536, 771
BspANI GGCC 5 cut(s) 15, 268, 370, 560, 872
BspCNI CTCAG 2 cut(s) 547, 730
BspFNI CGCG 2 cut(s) 257, 536
BspLI GGNNCC 2 cut(s) 240, 873
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 2 cut(s) 623, 851
BspOI GCTAGC 1 cut(s) 255
BsrI ACTGG 1 cut(s) 774
BssECI CCNNGG 2 cut(s) 547, 719
BssT1I CCWWGG 1 cut(s) 719
BstBAI YACGTR 2 cut(s) 106, 608
BstC8I GCNNGC 4 cut(s) 166, 253, 372, 726
BstDEI CTNAG 6 cut(s) 55, 243, 474, 504, 555, 738
BstDSI CCRYGG 1 cut(s) 547
BstF5I GGATG 4 cut(s) 100, 412, 767, 862
BstFNI CGCG 2 cut(s) 257, 536
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 3 cut(s) 82, 257, 536
BstMWI GCNNNNNNNGC 3 cut(s) 115, 380, 734
BstNSI RCATGY 2 cut(s) 642, 932
BstSCI CCNGG 1 cut(s) 813
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 2 cut(s) 257, 536
BstV1I GCAGC 3 cut(s) 96, 99, 895
BstV2I GAAGAC 2 cut(s) 471, 933
BsuRI GGCC 5 cut(s) 15, 268, 370, 560, 872
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 4 cut(s) 100, 412, 767, 862
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 342, 781
BveI ACCTGC 2 cut(s) 623, 851
Cac8I GCNNGC 4 cut(s) 166, 253, 372, 726
CfoI GCGC 3 cut(s) 82, 257, 536
Cfr13I GGNCC 2 cut(s) 465, 871
Csp6I GTAC 5 cut(s) 101, 290, 388, 636, 984
CviAII CATG 6 cut(s) 44, 264, 469, 562, 639, 929
CviQI GTAC 5 cut(s) 101, 290, 388, 636, 984
DdeI CTNAG 6 cut(s) 55, 243, 474, 504, 555, 738
DraI TTTAAA 1 cut(s) 654
EaeI YGGCCR 1 cut(s) 266
EciI GGCGGA 1 cut(s) 760
Eco130I CCWWGG 1 cut(s) 719
Eco147I AGGCCT 1 cut(s) 370
Eco24I GRGCYC 1 cut(s) 515
Eco47I GGWCC 1 cut(s) 465
Eco57I CTGAAG 1 cut(s) 300
Eco72I CACGTG 1 cut(s) 106
Eco88I CYCGRG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 719
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 719
FaeI CATG 6 cut(s) 47, 267, 472, 565, 642, 932
FatI CATG 6 cut(s) 43, 263, 468, 561, 638, 928
FauI CCCGC 1 cut(s) 83
FauNDI CATATG 1 cut(s) 888
Fnu4HI GCNGC 4 cut(s) 13, 88, 110, 909
FokI GGATG 4 cut(s) 107, 419, 754, 869
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 4 cut(s) 13, 88, 110, 909
FspBI CTAG 8 cut(s) 83, 192, 252, 299, 375, 420, 522, 704
GlaI GCGC 3 cut(s) 81, 256, 535
GluI GCNGC 4 cut(s) 13, 88, 110, 909
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 5 cut(s) 15, 268, 370, 560, 872
HapII CCGG 1 cut(s) 814
HhaI GCGC 3 cut(s) 82, 257, 536
Hin1II CATG 6 cut(s) 47, 267, 472, 565, 642, 932
Hin6I GCGC 3 cut(s) 80, 255, 534
HinP1I GCGC 3 cut(s) 80, 255, 534
HindIII AAGCTT 1 cut(s) 123
HpaII CCGG 1 cut(s) 814
HphI GGTGA 5 cut(s) 163, 469, 500, 823, 985
Hpy166II GTNNAC 2 cut(s) 103, 781
Hpy188I TCNGA 2 cut(s) 319, 366
Hpy188III TCNNGA 2 cut(s) 74, 192
Hpy8I GTNNAC 2 cut(s) 103, 781
HpyAV CCTTC 3 cut(s) 50, 524, 710
HpyCH4IV ACGT 2 cut(s) 105, 607
HpyCH4V TGCA 5 cut(s) 4, 308, 632, 767, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 380, 734
HpyF3I CTNAG 6 cut(s) 55, 243, 474, 504, 555, 738
HpySE526I ACGT 2 cut(s) 105, 607
Hsp92II CATG 6 cut(s) 47, 267, 472, 565, 642, 932
HspAI GCGC 3 cut(s) 80, 255, 534
Lsp1109I GCAGC 3 cut(s) 96, 99, 895
LweI GCATC 4 cut(s) 397, 450, 776, 847
MaeI CTAG 8 cut(s) 83, 192, 252, 299, 375, 420, 522, 704
MaeII ACGT 2 cut(s) 105, 607
MaeIII GTNAC 7 cut(s) 23, 301, 457, 564, 601, 608, 817
MboII GAAGA 4 cut(s) 306, 471, 933, 981
MhlI GDGCHC 2 cut(s) 515, 626
MlsI TGGCCA 1 cut(s) 268
MluCI AATT 1 cut(s) 848
MluNI TGGCCA 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 9 cut(s) 70, 184, 225, 360, 550, 609, 821, 885, 964
Mox20I TGGCCA 1 cut(s) 268
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 268
MseI TTAA 1 cut(s) 653
MslI CAYNNNNRTG 1 cut(s) 42
Msp20I TGGCCA 1 cut(s) 268
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 1 cut(s) 814
MspR9I CCNGG 1 cut(s) 815
MvnI CGCG 2 cut(s) 257, 536
MwoI GCNNNNNNNGC 3 cut(s) 115, 380, 734
NciI CCSGG 1 cut(s) 815
NdeI CATATG 1 cut(s) 888
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 6 cut(s) 47, 267, 472, 565, 642, 932
NlaIV GGNNCC 2 cut(s) 240, 873
NmuCI GTSAC 3 cut(s) 23, 457, 601
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 642, 932
PaeR7I CTCGAG 1 cut(s) 436
PceI AGGCCT 1 cut(s) 370
PciI ACATGT 1 cut(s) 638
PcsI WCGNNNNNNNCGW 2 cut(s) 254, 604
PkrI GCNGC 4 cut(s) 14, 89, 111, 910
PmaCI CACGTG 1 cut(s) 106
PmlI CACGTG 1 cut(s) 106
Ppu21I YACGTR 2 cut(s) 106, 608
PscI ACATGT 1 cut(s) 638
PspCI CACGTG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 240, 873
PspPI GGNCC 2 cut(s) 465, 871
PspXI VCTCGAGB 1 cut(s) 436
PstI CTGCAG 1 cut(s) 634
RsaI GTAC 5 cut(s) 102, 291, 389, 637, 985
RsaNI GTAC 5 cut(s) 101, 290, 388, 636, 984
RseI CAYNNNNRTG 1 cut(s) 42
SaqAI TTAA 1 cut(s) 653
SatI GCNGC 4 cut(s) 13, 88, 110, 909
Sau96I GGNCC 2 cut(s) 465, 871
ScrFI CCNGG 1 cut(s) 815
SduI GDGCHC 2 cut(s) 515, 626
SfaNI GCATC 4 cut(s) 397, 450, 776, 847
SfcI CTRYAG 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 436
SinI GGWCC 1 cut(s) 465
SlaI CTCGAG 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 42
SmlI CTYRAG 2 cut(s) 436, 805
SmoI CTYRAG 2 cut(s) 436, 805
Sse9I AATT 1 cut(s) 848
SseBI AGGCCT 1 cut(s) 370
SsiI CCGC 5 cut(s) 12, 90, 150, 536, 771
SspMI CTAG 8 cut(s) 83, 192, 252, 299, 375, 420, 522, 704
StuI AGGCCT 1 cut(s) 370
StyD4I CCNGG 1 cut(s) 813
StyI CCWWGG 1 cut(s) 719
TaiI ACGT 2 cut(s) 108, 610
TaqI TCGA 4 cut(s) 230, 437, 444, 690
TasI AATT 1 cut(s) 848
TauI GCSGC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 653
Tru9I TTAA 1 cut(s) 653
TscAI CASTG 2 cut(s) 349, 781
TseFI GTSAC 3 cut(s) 23, 457, 601
TseI GCWGC 3 cut(s) 87, 109, 908
Tsp45I GTSAC 3 cut(s) 23, 457, 601
TspDTI ATGAA 3 cut(s) 32, 471, 903
TspGWI ACGGA 1 cut(s) 934
TspRI CASTG 2 cut(s) 349, 781
VpaK11BI GGWCC 1 cut(s) 465
XbaI TCTAGA 1 cut(s) 191
XceI RCATGY 2 cut(s) 642, 932
XhoI CTCGAG 1 cut(s) 436
XspI CTAG 8 cut(s) 83, 192, 252, 299, 375, 420, 522, 704
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.