pycom06g09060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0017374
Physical Location & Seq
Forward (+)
14252535 .. 14254035
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g09060.5

Sequence Viewer

Length: 1101 bp
ATGATGCGAGTGATGTCGCGTTTCTCCTCTGCCAGATCTAAGTTTGTGGGCATCTCCTTACTGTTCTGCCCAATGCTTGGCAGCCAAAGAGAAAGGAGTTTCACTGGTAGCTCATCGTTTGGTGGTGTGATACGTCCACAAACATCTGGGGAGTTCATCTGGCCATTTTCCCTTCTTGTTGAAGAGGGACTTCTTGAGTACCTTAAGGATATCGGTGTGGACATGTGTTGCTTGATGCTGCTTGATGCCATACTTTTGGAAGAACATCACCAAATCTTTGCCTACGAATTGCAGGGCGTTGTCTGTGATGATGGATTGAGGGATGCCAAATCAGCAAATAATGTTCCTCCACATGAAGCGTTTTATGTTCATTTGTATCATCGTAGTCATAGGCTCTGCTTCTATCCATTTGGTGAAGTAATCGGTTGTCACGATCATCATGCCTTTGCCCCCAGTAGTGGGCAGCATTGGCCCTACCAAGTCGATTGTCCACTGCATGAACGACCAAAGGCTCATCTGTTGGCAACGATCATACTTTTTAGCCTGCGTTTAGAGCCTTCTGTGCTAAAGATCGACCTCCGGAGTGATTTCCACAAATGCCTTCATGGATTGAGCGTAGAACTTTTAGGTCAATTTTCGGACGAGAATGTCGTTCCACATATAATAGCGTGTTGCTTATATTTAGAGCTTTCTAGACTCCAATCTTTCCATGGGGAGTGTGCCATTGGCCAGGTAGTTTATAATGGATTCTTGCCAGTTTGGAATTGTACTAACCTATACTCCACCGGAATAGGGTGTTTGAGTTGGTGGTTGAGGGCGAAGCCTAGGCCGGCTAGTGCGTCCGCTGCGAGCTTTTTCATTGCCAAGTCTTTTACCATTCGAAGGCCTGCTAGTAGAGCCTCATACTCTACTTCGTTGTTGGATGTTTTGACACCTATAGTGATCACCTGCTCAGGCATCAAACCATCTAGGGCGTCGTCGACATGCAAATACCATAAGTCTCTATCGGGTGGAGCAGACGTAGCTAGGGCATGCTCGGCTATCTCCGGGGCATCGTTGAGCATCTCTGTTTTGACACATAGGCTAGGTGTGAAGGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
DUF223 Rep_fac-A_C

Protein Analysis

367

Amino Acids

40.48

Weight (kDa)

8.3

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 741
AarI CACCTGC 1 cut(s) 956
AasI GACNNNNNNGTC 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 956
AccB7I CCANNNNNTGG 1 cut(s) 77
AccI GTMKAC 1 cut(s) 980
AccII CGCG 1 cut(s) 19
AccIII TCCGGA 1 cut(s) 579
AciI CCGC 1 cut(s) 843
AcoI YGGCCR 2 cut(s) 161, 727
AcyI GRCGYC 1 cut(s) 974
AfaI GTAC 2 cut(s) 200, 769
AfiI CCNNNNNNNGG 5 cut(s) 77, 458, 459, 792, 882
AflII CTTAAG 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 1 cut(s) 182
AjnI CCWGG 1 cut(s) 729
AluBI AGCT 4 cut(s) 111, 688, 852, 1025
AluI AGCT 4 cut(s) 111, 688, 852, 1025
Alw26I GTCTC 1 cut(s) 1005
Aor13HI TCCGGA 1 cut(s) 579
AoxI GGCC 5 cut(s) 161, 470, 727, 827, 884
ApeKI GCWGC 4 cut(s) 81, 238, 463, 845
AspA2I CCTAGG 1 cut(s) 824
AspS9I GGNCC 1 cut(s) 471
AsuC2I CCSGG 1 cut(s) 1048
AsuHPI GGTGA 3 cut(s) 260, 425, 937
AsuII TTCGAA 1 cut(s) 880
AvrII CCTAGG 1 cut(s) 824
BalI TGGCCA 2 cut(s) 163, 729
BbvI GCAGC 4 cut(s) 93, 225, 475, 832
BccI CCATC 2 cut(s) 305, 973
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 1 cut(s) 731
BclI TGATCA 1 cut(s) 942
BcnI CCSGG 1 cut(s) 1048
BcoDI GTCTC 1 cut(s) 1005
BfaI CTAG 7 cut(s) 693, 825, 834, 891, 969, 1026, 1085
BfmI CTRYAG 1 cut(s) 936
BfrI CTTAAG 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 956
BglII AGATCT 1 cut(s) 35
BisI GCNGC 4 cut(s) 82, 239, 464, 846
BlnI CCTAGG 1 cut(s) 824
BlsI GCNGC 4 cut(s) 83, 240, 465, 847
Bme1390I CCNGG 2 cut(s) 731, 1048
BmgT120I GGNCC 1 cut(s) 471
BmrFI CCNGG 2 cut(s) 731, 1048
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 7 cut(s) 60, 225, 235, 313, 966, 1061, 1071
BmuI ACTGGG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 952
Bpu14I TTCGAA 1 cut(s) 880
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 1048
BsaHI GRCGYC 1 cut(s) 974
BsaJI CCNNGG 3 cut(s) 709, 824, 1047
BsaWI WCCGGW 2 cut(s) 579, 785
BsaXI ACNNNNNCTCC 4 cut(s) 764, 794, 1005, 1035
Bsc4I CCNNNNNNNGG 5 cut(s) 77, 458, 459, 792, 882
Bse118I RCCGGY 1 cut(s) 829
Bse1I ACTGG 3 cut(s) 109, 453, 755
Bse3DI GCAATG 1 cut(s) 858
BseAI TCCGGA 1 cut(s) 579
BseBI CCWGG 1 cut(s) 731
BseDI CCNNGG 3 cut(s) 709, 824, 1047
BseGI GGATG 2 cut(s) 328, 928
BseLI CCNNNNNNNGG 5 cut(s) 77, 458, 459, 792, 882
BseMI GCAATG 1 cut(s) 858
BseMII CTCAG 1 cut(s) 966
BseNI ACTGG 3 cut(s) 109, 453, 755
BseRI GAGGAG 1 cut(s) 16
BseXI GCAGC 4 cut(s) 93, 225, 475, 832
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 5 cut(s) 163, 472, 729, 829, 886
BsiSI CCGG 4 cut(s) 580, 786, 830, 1047
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 5 cut(s) 77, 458, 459, 792, 882
BsmAI GTCTC 1 cut(s) 1005
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 5 cut(s) 163, 472, 729, 829, 886
Bsp119I TTCGAA 1 cut(s) 880
Bsp13I TCCGGA 1 cut(s) 579
Bsp143I GATC 5 cut(s) 35, 433, 528, 570, 942
Bsp19I CCATGG 1 cut(s) 709
BspACI CCGC 1 cut(s) 843
BspANI GGCC 5 cut(s) 163, 472, 729, 829, 886
BspCNI CTCAG 1 cut(s) 965
BspEI TCCGGA 1 cut(s) 579
BspFNI CGCG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 956
BspT104I TTCGAA 1 cut(s) 880
BspTI CTTAAG 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 858
BsrFI RCCGGY 1 cut(s) 829
BsrI ACTGG 3 cut(s) 109, 453, 755
BssAI RCCGGY 1 cut(s) 829
BssECI CCNNGG 3 cut(s) 709, 824, 1047
BssMI GATC 5 cut(s) 35, 433, 528, 570, 942
BssNI GRCGYC 1 cut(s) 974
BssT1I CCWWGG 2 cut(s) 709, 824
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 974
BstAFI CTTAAG 1 cut(s) 203
BstBI TTCGAA 1 cut(s) 880
BstC8I GCNNGC 5 cut(s) 545, 831, 850, 888, 1033
BstDEI CTNAG 2 cut(s) 39, 952
BstDSI CCRYGG 1 cut(s) 709
BstF5I GGATG 2 cut(s) 328, 928
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 5 cut(s) 38, 436, 531, 573, 945
BstMAI GTCTC 1 cut(s) 1005
BstMBI GATC 5 cut(s) 35, 433, 528, 570, 942
BstMWI GCNNNNNNNGC 8 cut(s) 332, 469, 553, 562, 845, 896, 1022, 1037
BstNI CCWGG 1 cut(s) 731
BstNSI RCATGY 3 cut(s) 226, 987, 1035
BstSCI CCNGG 2 cut(s) 729, 1046
BstSFI CTRYAG 1 cut(s) 936
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 4 cut(s) 93, 225, 475, 832
BstX2I RGATCY 1 cut(s) 35
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 5 cut(s) 163, 472, 729, 829, 886
BtgI CCRYGG 1 cut(s) 709
BtsCI GGATG 2 cut(s) 328, 928
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 2 cut(s) 102, 491
BveI ACCTGC 1 cut(s) 956
Cac8I GCNNGC 5 cut(s) 545, 831, 850, 888, 1033
Cfr10I RCCGGY 1 cut(s) 829
Cfr13I GGNCC 1 cut(s) 471
CseI GACGC 2 cut(s) 828, 963
Csp6I GTAC 2 cut(s) 199, 768
CviAII CATG 8 cut(s) 223, 353, 440, 497, 605, 710, 984, 1032
CviQI GTAC 2 cut(s) 199, 768
DdeI CTNAG 2 cut(s) 39, 952
DpnI GATC 5 cut(s) 37, 435, 530, 572, 944
DpnII GATC 5 cut(s) 35, 433, 528, 570, 942
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
EaeI YGGCCR 2 cut(s) 161, 727
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 709, 824
Eco147I AGGCCT 1 cut(s) 886
Eco32I GATATC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 729
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 709, 824
ErhI CCWWGG 2 cut(s) 709, 824
FaeI CATG 8 cut(s) 226, 356, 443, 500, 608, 713, 987, 1035
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 1 cut(s) 201
FatI CATG 8 cut(s) 222, 352, 439, 496, 604, 709, 983, 1031
FbaI TGATCA 1 cut(s) 942
FblI GTMKAC 1 cut(s) 980
Fnu4HI GCNGC 4 cut(s) 82, 239, 464, 846
FokI GGATG 2 cut(s) 335, 935
Fsp4HI GCNGC 4 cut(s) 82, 239, 464, 846
FspBI CTAG 7 cut(s) 693, 825, 834, 891, 969, 1026, 1085
GluI GCNGC 4 cut(s) 82, 239, 464, 846
HaeIII GGCC 5 cut(s) 163, 472, 729, 829, 886
HapII CCGG 4 cut(s) 580, 786, 830, 1047
HgaI GACGC 2 cut(s) 828, 963
Hin1I GRCGYC 1 cut(s) 974
Hin1II CATG 8 cut(s) 226, 356, 443, 500, 608, 713, 987, 1035
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HinfI GANTC 2 cut(s) 696, 747
HpaII CCGG 4 cut(s) 580, 786, 830, 1047
HphI GGTGA 3 cut(s) 260, 425, 937
Hpy166II GTNNAC 4 cut(s) 137, 220, 491, 981
Hpy188I TCNGA 1 cut(s) 640
Hpy188III TCNNGA 4 cut(s) 194, 431, 580, 693
Hpy8I GTNNAC 4 cut(s) 137, 220, 491, 981
Hpy99I CGWCG 2 cut(s) 979, 982
HpyAV CCTTC 5 cut(s) 182, 567, 611, 876, 1087
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 2 cut(s) 133, 1020
HpyCH4V TGCA 3 cut(s) 292, 496, 987
HpyF10VI GCNNNNNNNGC 8 cut(s) 332, 469, 553, 562, 845, 896, 1022, 1037
HpyF3I CTNAG 2 cut(s) 39, 952
HpySE526I ACGT 2 cut(s) 133, 1020
Hsp92I GRCGYC 1 cut(s) 974
Hsp92II CATG 8 cut(s) 226, 356, 443, 500, 608, 713, 987, 1035
Kpn2I TCCGGA 1 cut(s) 579
KroI GCCGGC 1 cut(s) 829
KroNI GCCGGC 1 cut(s) 831
Ksp22I TGATCA 1 cut(s) 942
Kzo9I GATC 5 cut(s) 35, 433, 528, 570, 942
LmnI GCTCC 1 cut(s) 1013
Lsp1109I GCAGC 4 cut(s) 93, 225, 475, 832
LweI GCATC 7 cut(s) 60, 225, 235, 313, 966, 1061, 1071
MaeI CTAG 7 cut(s) 693, 825, 834, 891, 969, 1026, 1085
MaeII ACGT 2 cut(s) 133, 1020
MaeIII GTNAC 1 cut(s) 428
MalI GATC 5 cut(s) 37, 435, 530, 572, 944
MboI GATC 5 cut(s) 35, 433, 528, 570, 942
MboII GAAGA 2 cut(s) 194, 272
MflI RGATCY 1 cut(s) 35
MlsI TGGCCA 2 cut(s) 163, 729
MluCI AATT 3 cut(s) 287, 632, 763
MluNI TGGCCA 2 cut(s) 163, 729
MlyI GAGTC 1 cut(s) 690
MmeI TCCRAC 1 cut(s) 900
MnlI CCTC 7 cut(s) 37, 178, 312, 357, 587, 807, 910
Mox20I TGGCCA 2 cut(s) 163, 729
MroI TCCGGA 1 cut(s) 579
MroNI GCCGGC 1 cut(s) 829
MscI TGGCCA 2 cut(s) 163, 729
MseI TTAA 1 cut(s) 204
Msp20I TGGCCA 2 cut(s) 163, 729
MspA1I CMGCKG 1 cut(s) 845
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 4 cut(s) 580, 786, 830, 1047
MspR9I CCNGG 2 cut(s) 731, 1048
MvaI CCWGG 1 cut(s) 731
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 8 cut(s) 332, 469, 553, 562, 845, 896, 1022, 1037
NaeI GCCGGC 1 cut(s) 831
NciI CCSGG 1 cut(s) 1048
NcoI CCATGG 1 cut(s) 709
NdeII GATC 5 cut(s) 35, 433, 528, 570, 942
NgoMIV GCCGGC 1 cut(s) 829
NlaIII CATG 8 cut(s) 226, 356, 443, 500, 608, 713, 987, 1035
NmeAIII GCCGAG 1 cut(s) 1016
NmuCI GTSAC 1 cut(s) 428
NspI RCATGY 3 cut(s) 226, 987, 1035
NspV TTCGAA 1 cut(s) 880
PaeI GCATGC 1 cut(s) 1035
PaqCI CACCTGC 1 cut(s) 956
PceI AGGCCT 1 cut(s) 886
PciI ACATGT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 2 cut(s) 429, 648
PdiI GCCGGC 1 cut(s) 831
PfeI GAWTC 1 cut(s) 747
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 4 cut(s) 83, 240, 465, 847
PleI GAGTC 1 cut(s) 690
PpsI GAGTC 1 cut(s) 690
PscI ACATGT 1 cut(s) 222
PsiI TTATAA 1 cut(s) 741
Psp6I CCWGG 1 cut(s) 729
PspGI CCWGG 1 cut(s) 729
PspPI GGNCC 1 cut(s) 471
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 2 cut(s) 200, 769
RsaNI GTAC 2 cut(s) 199, 768
SalI GTCGAC 1 cut(s) 979
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 4 cut(s) 82, 239, 464, 846
Sau3AI GATC 5 cut(s) 35, 433, 528, 570, 942
Sau96I GGNCC 1 cut(s) 471
SchI GAGTC 1 cut(s) 690
ScrFI CCNGG 2 cut(s) 731, 1048
SfaNI GCATC 7 cut(s) 60, 225, 235, 313, 966, 1061, 1071
SfcI CTRYAG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 880
SmlI CTYRAG 2 cut(s) 194, 203
SmoI CTYRAG 2 cut(s) 194, 203
SphI GCATGC 1 cut(s) 1035
Sse9I AATT 3 cut(s) 287, 632, 763
SseBI AGGCCT 1 cut(s) 886
SsiI CCGC 1 cut(s) 843
SspMI CTAG 7 cut(s) 693, 825, 834, 891, 969, 1026, 1085
StuI AGGCCT 1 cut(s) 886
StyD4I CCNGG 2 cut(s) 729, 1046
StyI CCWWGG 2 cut(s) 709, 824
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 2 cut(s) 136, 1023
TaqI TCGA 4 cut(s) 483, 573, 880, 980
TasI AATT 3 cut(s) 287, 632, 763
TatI WGTACW 1 cut(s) 767
TfiI GAWTC 1 cut(s) 747
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 109, 498
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 4 cut(s) 81, 238, 463, 845
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 6 cut(s) 145, 359, 369, 513, 593, 847
TspRI CASTG 2 cut(s) 109, 498
Van91I CCANNNNNTGG 1 cut(s) 77
Vha464I CTTAAG 1 cut(s) 203
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 3 cut(s) 226, 987, 1035
XcmI CCANNNNNNNNNTGG 1 cut(s) 707
XmaJI CCTAGG 1 cut(s) 824
XmiI GTMKAC 1 cut(s) 980
XspI CTAG 7 cut(s) 693, 825, 834, 891, 969, 1026, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.