pycom01g03700

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0017374
Physical Location & Seq
Forward (+)
2787067 .. 2787875
809 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03700.5

Sequence Viewer

Length: 624 bp
ATGCTCAATTGCCAGTATTGGGCCACTTTAGAGTTGAATTATGAAGTAAGGGTCGGCTGGGGCTGTGTACCATGTAGCAGGAGATGGTGTTCGACTGCCCTTTGCGCACCTTTAGGTCGAGTTGTTGCGTTCGTTAGAAAATTTTGCATCCGCCAGTGTCTACGCCTTTATTGTGAAGCATCTGGATTGGACAGAATAGTGCGTCATGATTATGAGTACGTGCGTCTGAAGGTAAAACTTGAGCTTCCGGGTTGCAACAATTATCGCCAAAGTAATCGGGCCCCCAACTCTTCTCGTATGATGGTAGAGCTTATTGCTGCTTTAGATACTGTCAAACATGTGAATAGTTCATCTGCTCCTTCCGGTTTGGATAGTAGGGAGGTGATCCTTGTCCCAAATTTTTTTTTTCATGATCAATTTTCCAAATCAGCCAGTGGTCTGCTGGAAGTCCTTTTTGGAAGGAGATTTTTTTTTTTTCTTGATCAATTTTCCAAATTAGCAAGTGGTCTGTTGGAAGTCCTTTTTGGAAGGCTGCTCTACTTGCTCAATCTCGTCTGTGAAGGTGACCTCGTTGCATTGGAAATTGCCAATCTCTTAGTCAAGAGTCTCTTTACTTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

208

Amino Acids

23.55

Weight (kDa)

8.84

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 106
AccI GTMKAC 1 cut(s) 160
AciI CCGC 1 cut(s) 151
AclWI GGATC 1 cut(s) 379
AcsI RAATTY 2 cut(s) 140, 397
AcuI CTGAAG 1 cut(s) 248
AfaI GTAC 2 cut(s) 69, 218
AfiI CCNNNNNNNGG 1 cut(s) 19
AflIII ACRYGT 1 cut(s) 337
AgsI TTSAA 1 cut(s) 37
AjuI GAANNNNNNNTTGG 4 cut(s) 438, 470, 507, 539
AloI GAACNNNNNNTCC 2 cut(s) 73, 105
AluBI AGCT 2 cut(s) 244, 310
AluI AGCT 2 cut(s) 244, 310
Alw26I GTCTC 1 cut(s) 611
AlwI GGATC 1 cut(s) 379
AoxI GGCC 2 cut(s) 21, 279
ApaI GGGCCC 1 cut(s) 283
ApeKI GCWGC 2 cut(s) 317, 532
ApoI RAATTY 2 cut(s) 140, 397
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 3 cut(s) 21, 279, 280
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 2 cut(s) 394, 575
BaeGI GKGCMC 1 cut(s) 283
BanII GRGCYC 1 cut(s) 283
BbvI GCAGC 2 cut(s) 304, 519
BccI CCATC 2 cut(s) 78, 295
BclI TGATCA 2 cut(s) 412, 481
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 611
BisI GCNGC 2 cut(s) 318, 533
BlsI GCNGC 2 cut(s) 319, 534
Bme1390I CCNGG 1 cut(s) 249
BmgT120I GGNCC 3 cut(s) 21, 279, 280
BmiI GGNNCC 2 cut(s) 281, 282
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 156, 188
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 249
BsaAI YACGTR 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 362
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse1I ACTGG 3 cut(s) 13, 154, 432
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 1 cut(s) 19
BseNI ACTGG 3 cut(s) 13, 154, 432
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 2 cut(s) 304, 519
BseYI CCCAGC 1 cut(s) 57
BshFI GGCC 2 cut(s) 23, 281
BsiSI CCGG 2 cut(s) 248, 363
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 611
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 23, 281
Bsp120I GGGCCC 1 cut(s) 279
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 3 cut(s) 384, 412, 481
BspACI CCGC 1 cut(s) 151
BspANI GGCC 2 cut(s) 23, 281
BspHI TCATGA 2 cut(s) 205, 409
BspLI GGNNCC 2 cut(s) 281, 282
BspPI GGATC 1 cut(s) 379
BsrI ACTGG 3 cut(s) 13, 154, 432
BssMI GATC 3 cut(s) 384, 412, 481
Bst4CI ACNGT 1 cut(s) 331
Bst6I CTCTTC 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 220
BstDEI CTNAG 1 cut(s) 595
BstEII GGTNACC 1 cut(s) 563
BstF5I GGATG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 3 cut(s) 387, 415, 484
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 3 cut(s) 384, 412, 481
BstMWI GCNNNNNNNGC 2 cut(s) 104, 541
BstNSI RCATGY 1 cut(s) 341
BstPI GGTNACC 1 cut(s) 563
BstSCI CCNGG 1 cut(s) 247
BstSLI GKGCMC 1 cut(s) 283
BstV1I GCAGC 2 cut(s) 304, 519
BsuRI GGCC 2 cut(s) 23, 281
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 161, 439
CciI TCATGA 2 cut(s) 205, 409
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 3 cut(s) 21, 279, 280
CseI GACGC 2 cut(s) 191, 212
Csp6I GTAC 2 cut(s) 68, 217
CviAII CATG 4 cut(s) 72, 206, 338, 410
CviJI RGCY 8 cut(s) 23, 57, 63, 244, 281, 310, 431, 532
CviKI_1 RGCY 8 cut(s) 23, 57, 63, 244, 281, 310, 431, 532
CviQI GTAC 2 cut(s) 68, 217
DdeI CTNAG 1 cut(s) 595
DpnI GATC 3 cut(s) 386, 414, 483
DpnII GATC 3 cut(s) 384, 412, 481
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
EciI GGCGGA 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 248
Eco91I GGTNACC 1 cut(s) 563
EcoO109I RGGNCCY 1 cut(s) 280
EcoO65I GGTNACC 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 283
FaeI CATG 4 cut(s) 75, 209, 341, 413
FaiI YATR 7 cut(s) 42, 73, 207, 213, 299, 339, 411
FalI AAGNNNNNCTT 1 cut(s) 593
FaqI GGGAC 1 cut(s) 377
FatI CATG 4 cut(s) 71, 205, 337, 409
FbaI TGATCA 2 cut(s) 412, 481
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 318, 533
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 2 cut(s) 318, 533
FspI TGCGCA 1 cut(s) 106
GlaI GCGC 1 cut(s) 106
GluI GCNGC 2 cut(s) 318, 533
GsaI CCCAGC 1 cut(s) 61
HaeIII GGCC 2 cut(s) 23, 281
HapII CCGG 2 cut(s) 248, 363
HgaI GACGC 2 cut(s) 191, 212
HhaI GCGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 75, 209, 341, 413
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 1 cut(s) 604
HpaII CCGG 2 cut(s) 248, 363
HphI GGTGA 2 cut(s) 394, 575
Hpy166II GTNNAC 2 cut(s) 68, 161
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 6 cut(s) 183, 206, 410, 479, 601, 621
Hpy8I GTNNAC 2 cut(s) 68, 161
HpyAV CCTTC 5 cut(s) 223, 369, 453, 522, 554
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 3 cut(s) 147, 255, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 541
HpyF3I CTNAG 1 cut(s) 595
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 4 cut(s) 75, 209, 341, 413
HspAI GCGC 1 cut(s) 105
Ksp22I TGATCA 2 cut(s) 412, 481
Kzo9I GATC 3 cut(s) 384, 412, 481
LmnI GCTCC 1 cut(s) 361
LpnPI CCDG 9 cut(s) 26, 43, 64, 167, 168, 261, 376, 428, 445
Lsp1109I GCAGC 2 cut(s) 304, 519
LweI GCATC 2 cut(s) 156, 188
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 563
MalI GATC 3 cut(s) 386, 414, 483
MboI GATC 3 cut(s) 384, 412, 481
MboII GAAGA 2 cut(s) 282, 609
MfeI CAATTG 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 9 cut(s) 7, 37, 140, 259, 397, 416, 485, 494, 582
MlyI GAGTC 1 cut(s) 613
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 2 cut(s) 373, 578
MslI CAYNNNNRTG 1 cut(s) 210
MspI CCGG 2 cut(s) 248, 363
MspR9I CCNGG 1 cut(s) 249
MunI CAATTG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 104, 541
NciI CCSGG 1 cut(s) 249
NdeII GATC 3 cut(s) 384, 412, 481
NlaIII CATG 4 cut(s) 75, 209, 341, 413
NlaIV GGNNCC 2 cut(s) 281, 282
NmuCI GTSAC 1 cut(s) 563
NsbI TGCGCA 1 cut(s) 106
NspI RCATGY 1 cut(s) 341
PagI TCATGA 2 cut(s) 205, 409
PciI ACATGT 1 cut(s) 337
PkrI GCNGC 2 cut(s) 319, 534
PleI GAGTC 1 cut(s) 612
PpsI GAGTC 1 cut(s) 612
Ppu21I YACGTR 1 cut(s) 220
PscI ACATGT 1 cut(s) 337
PspEI GGTNACC 1 cut(s) 563
PspFI CCCAGC 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 281, 282
PspOMI GGGCCC 1 cut(s) 279
PspPI GGNCC 3 cut(s) 21, 279, 280
RsaI GTAC 2 cut(s) 69, 218
RsaNI GTAC 2 cut(s) 68, 217
RseI CAYNNNNRTG 1 cut(s) 210
SatI GCNGC 2 cut(s) 318, 533
Sau3AI GATC 3 cut(s) 384, 412, 481
Sau96I GGNCC 3 cut(s) 21, 279, 280
SchI GAGTC 1 cut(s) 613
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 283
SetI ASST 9 cut(s) 112, 118, 222, 234, 246, 312, 384, 565, 570
SfaNI GCATC 2 cut(s) 156, 188
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 9 cut(s) 7, 37, 140, 259, 397, 416, 485, 494, 582
SsiI CCGC 1 cut(s) 151
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 92, 118
TasI AATT 9 cut(s) 7, 37, 140, 259, 397, 416, 485, 494, 582
TscAI CASTG 2 cut(s) 161, 439
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 2 cut(s) 317, 532
Tsp45I GTSAC 1 cut(s) 563
TspDTI ATGAA 3 cut(s) 57, 339, 398
TspRI CASTG 2 cut(s) 161, 439
XapI RAATTY 2 cut(s) 140, 397
XceI RCATGY 1 cut(s) 341
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.