pycom13g23440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0017374
Physical Location & Seq
Forward (+)
20976575 .. 20977490
916 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g23440.2

Sequence Viewer

Length: 753 bp
ATGTGGGTGATAGTATGCTCACGCTTCTCCTCTGCCAGATCTAAGCTTGTGGCCATCTCCTTACTGTTCTGCTCAATGCTTGGTAGTAGAGCGATGATACTTGGCCAAAGAGAAAGGAGTCTCACCGGTTGCTCGTCTTTTGGTGTTACGATATGCCCATTGATATCCGGGGAGTTCGTCTGGCCATTTTCCCTTCTTGTTGGTGAGGGATTTCTTGAGGCAGTCGAGGATCATCTTGTTGGATGCTTCATCCCACCTATTACGTTGAAGATATCTCGATGTGGACATGTGCTGCTTAATGCCATACTTTTGGAAGAACTTCGCCATAACTTTGCCCATGAATTGCTGGCCGTTGTTGGTGACAATGGACTAAGAGATGCCAAATCGACAAATTATGTTCCTCCATATAAAGTGCTCTATGTCCATTTAAACCATGGTCATCTTGGGCTCTGCTTCTACCTATTTGGTGAAGTAGTCGGTTGCCACGATCATCATGCTTCTGCCCCCAATAACAGCGCCAAGGACTCGTATACGGGTGTAGCTCACTGGCAGGCAGTGCTGGTATCGGCTTGTAAACCTTATGTGTTAAGGATCGACCTTCGGAGTGATTTCCACAAATGCCTTCGTAGATTGAGCTTAGAACCTTCAAGTCATTGGGAGGTGTTAGACAGTGGAGACGTGGTCCAGTCGTGCTGCCTTGATTTGGAGCTTCCTAGACTCCAATCTTTCTGTGGGGAATGTGTCATTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
DUF223 Rep_fac-A_C

Protein Analysis

251

Amino Acids

27.68

Weight (kDa)

6.34

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 530
AclWI GGATC 2 cut(s) 237, 599
AcoI YGGCCR 4 cut(s) 51, 103, 182, 348
AfiI CCNNNNNNNGG 1 cut(s) 703
AflIII ACRYGT 1 cut(s) 286
AgeI ACCGGT 1 cut(s) 125
AgsI TTSAA 2 cut(s) 268, 648
AjiI CACGTC 1 cut(s) 679
AluBI AGCT 4 cut(s) 46, 542, 636, 709
AluI AGCT 4 cut(s) 46, 542, 636, 709
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 125, 669
AlwI GGATC 2 cut(s) 237, 599
AoxI GGCC 4 cut(s) 51, 103, 182, 348
ApeKI GCWGC 2 cut(s) 292, 693
AsiGI ACCGGT 1 cut(s) 125
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 682
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 5 cut(s) 19, 115, 215, 371, 479
AvaII GGWCC 1 cut(s) 682
BalI TGGCCA 3 cut(s) 53, 105, 184
BanII GRGCYC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 2 cut(s) 279, 680
BccI CCATC 1 cut(s) 62
BceAI ACGGC 1 cut(s) 335
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 125, 669
BfaI CTAG 2 cut(s) 714, 751
BfoI RGCGCY 1 cut(s) 519
BglII AGATCT 1 cut(s) 38
BisI GCNGC 2 cut(s) 293, 694
BlsI GCNGC 2 cut(s) 294, 695
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 1 cut(s) 682
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 2 cut(s) 233, 367
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 169
BsaJI CCNNGG 3 cut(s) 168, 433, 519
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 1 cut(s) 703
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 551, 685
BseDI CCNNGG 3 cut(s) 168, 433, 519
BseGI GGATG 2 cut(s) 248, 249
BseLI CCNNNNNNNGG 1 cut(s) 703
BseNI ACTGG 2 cut(s) 551, 685
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 2 cut(s) 279, 680
BshFI GGCC 4 cut(s) 53, 105, 184, 350
BshTI ACCGGT 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 2 cut(s) 126, 168
BslI CCNNNNNNNGG 1 cut(s) 703
BsmAI GTCTC 2 cut(s) 125, 669
BsmBI CGTCTC 1 cut(s) 669
BsnI GGCC 4 cut(s) 53, 105, 184, 350
Bsp1286I GDGCHC 2 cut(s) 417, 450
Bsp143I GATC 4 cut(s) 38, 229, 487, 591
Bsp19I CCATGG 1 cut(s) 433
BspANI GGCC 4 cut(s) 53, 105, 184, 350
BspPI GGATC 2 cut(s) 237, 599
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 551, 685
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 3 cut(s) 168, 433, 519
BssMI GATC 4 cut(s) 38, 229, 487, 591
BssNAI GTATAC 1 cut(s) 531
BssT1I CCWWGG 2 cut(s) 433, 519
Bst1107I GTATAC 1 cut(s) 531
Bst4CI ACNGT 2 cut(s) 66, 671
BstAPI GCANNNNNTGC 1 cut(s) 556
BstC8I GCNNGC 2 cut(s) 348, 552
BstDEI CTNAG 3 cut(s) 42, 371, 637
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 2 cut(s) 248, 249
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 4 cut(s) 41, 232, 490, 594
BstMAI GTCTC 2 cut(s) 125, 669
BstMBI GATC 4 cut(s) 38, 229, 487, 591
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 167
BstV1I GCAGC 2 cut(s) 279, 680
BstX2I RGATCY 1 cut(s) 38
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 38
BstZ17I GTATAC 1 cut(s) 531
BsuRI GGCC 4 cut(s) 53, 105, 184, 350
BtgI CCRYGG 1 cut(s) 433
BtgZI GCGATG 1 cut(s) 107
BtrI CACGTC 1 cut(s) 679
BtsCI GGATG 2 cut(s) 248, 249
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 3 cut(s) 544, 561, 676
Cac8I GCNNGC 2 cut(s) 348, 552
CfoI GCGC 1 cut(s) 518
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 682
CspAI ACCGGT 1 cut(s) 125
CviAII CATG 4 cut(s) 287, 338, 434, 494
DdeI CTNAG 3 cut(s) 42, 371, 637
DpnI GATC 4 cut(s) 40, 231, 489, 593
DpnII GATC 4 cut(s) 38, 229, 487, 591
DraI TTTAAA 1 cut(s) 429
EaeI YGGCCR 4 cut(s) 51, 103, 182, 348
Eco130I CCWWGG 2 cut(s) 433, 519
Eco24I GRGCYC 1 cut(s) 450
Eco32I GATATC 2 cut(s) 165, 273
Eco47I GGWCC 1 cut(s) 682
EcoRV GATATC 2 cut(s) 165, 273
EcoT14I CCWWGG 2 cut(s) 433, 519
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 2 cut(s) 433, 519
Esp3I CGTCTC 1 cut(s) 669
FaeI CATG 4 cut(s) 290, 341, 437, 497
FatI CATG 4 cut(s) 286, 337, 433, 493
FblI GTMKAC 1 cut(s) 530
Fnu4HI GCNGC 2 cut(s) 293, 694
FokI GGATG 2 cut(s) 236, 255
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 2 cut(s) 293, 694
FspBI CTAG 2 cut(s) 714, 751
GlaI GCGC 1 cut(s) 517
GluI GCNGC 2 cut(s) 293, 694
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 4 cut(s) 53, 105, 184, 350
HapII CCGG 2 cut(s) 126, 168
HhaI GCGC 1 cut(s) 518
Hin1II CATG 4 cut(s) 290, 341, 437, 497
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 3 cut(s) 118, 524, 717
HpaII CCGG 2 cut(s) 126, 168
HphI GGTGA 5 cut(s) 19, 115, 215, 371, 479
Hpy166II GTNNAC 3 cut(s) 284, 531, 575
Hpy188I TCNGA 1 cut(s) 603
Hpy188III TCNNGA 2 cut(s) 215, 276
Hpy8I GTNNAC 3 cut(s) 284, 531, 575
HpyAV CCTTC 4 cut(s) 203, 608, 632, 654
HpyCH4III ACNGT 2 cut(s) 66, 671
HpyCH4IV ACGT 2 cut(s) 263, 678
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 3 cut(s) 42, 371, 637
HpySE526I ACGT 2 cut(s) 263, 678
Hsp92II CATG 4 cut(s) 290, 341, 437, 497
HspAI GCGC 1 cut(s) 516
Kzo9I GATC 4 cut(s) 38, 229, 487, 591
LmnI GCTCC 1 cut(s) 706
LpnPI CCDG 9 cut(s) 49, 139, 166, 181, 332, 532, 536, 545, 698
Lsp1109I GCAGC 2 cut(s) 279, 680
LweI GCATC 2 cut(s) 233, 367
MaeI CTAG 2 cut(s) 714, 751
MaeII ACGT 2 cut(s) 263, 678
MaeIII GTNAC 2 cut(s) 145, 359
MalI GATC 4 cut(s) 40, 231, 489, 593
MboI GATC 4 cut(s) 38, 229, 487, 591
MboII GAAGA 2 cut(s) 280, 326
MflI RGATCY 1 cut(s) 38
MhlI GDGCHC 2 cut(s) 417, 450
MlsI TGGCCA 3 cut(s) 53, 105, 184
MluCI AATT 2 cut(s) 341, 391
MluNI TGGCCA 3 cut(s) 53, 105, 184
MlyI GAGTC 3 cut(s) 127, 518, 711
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 6 cut(s) 40, 199, 211, 220, 411, 652
Mox20I TGGCCA 3 cut(s) 53, 105, 184
MroXI GAANNNNTTC 1 cut(s) 318
MscI TGGCCA 3 cut(s) 53, 105, 184
MseI TTAA 3 cut(s) 297, 428, 587
Msp20I TGGCCA 3 cut(s) 53, 105, 184
MspI CCGG 2 cut(s) 126, 168
MspR9I CCNGG 1 cut(s) 169
MwoI GCNNNNNNNGC 1 cut(s) 556
NciI CCSGG 1 cut(s) 169
NcoI CCATGG 1 cut(s) 433
NdeII GATC 4 cut(s) 38, 229, 487, 591
NlaIII CATG 4 cut(s) 290, 341, 437, 497
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 1 cut(s) 290
PciI ACATGT 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 318
PflFI GACNNNGTC 1 cut(s) 680
PinAI ACCGGT 1 cut(s) 125
PkrI GCNGC 2 cut(s) 294, 695
PleI GAGTC 3 cut(s) 126, 518, 711
PpsI GAGTC 3 cut(s) 126, 518, 711
PscI ACATGT 1 cut(s) 286
PspPI GGNCC 1 cut(s) 682
PsuI RGATCY 1 cut(s) 38
PsyI GACNNNGTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 297, 428, 587
SatI GCNGC 2 cut(s) 293, 694
Sau3AI GATC 4 cut(s) 38, 229, 487, 591
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 3 cut(s) 127, 518, 711
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 2 cut(s) 417, 450
SfaNI GCATC 2 cut(s) 233, 367
SinI GGWCC 1 cut(s) 682
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 341, 391
SspMI CTAG 2 cut(s) 714, 751
StyD4I CCNGG 1 cut(s) 167
StyI CCWWGG 2 cut(s) 433, 519
TaaI ACNGT 2 cut(s) 66, 671
TaiI ACGT 2 cut(s) 266, 681
TaqI TCGA 4 cut(s) 225, 277, 386, 594
TasI AATT 2 cut(s) 341, 391
Tru1I TTAA 3 cut(s) 297, 428, 587
Tru9I TTAA 3 cut(s) 297, 428, 587
TscAI CASTG 3 cut(s) 551, 561, 676
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 2 cut(s) 292, 693
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 2 cut(s) 238, 354
TspRI CASTG 3 cut(s) 551, 561, 676
Tth111I GACNNNGTC 1 cut(s) 680
VpaK11BI GGWCC 1 cut(s) 682
XceI RCATGY 1 cut(s) 290
XcmI CCANNNNNNNNNTGG 3 cut(s) 431, 440, 728
XmiI GTMKAC 1 cut(s) 530
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 2 cut(s) 714, 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.