MD12G1203600.v1.1

Chloroplast stem-loop binding protein of 41 kDa b

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008555
Physical Location & Seq
Reverse (-)
28380008 .. 28383244
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1203600.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGCAAGGTTGGTGGCTGTGCAACCGCAGCAGAGAAACCCATCTTTCTCTCTCCTCCCTCCTTCCTCTCTCTCTGACTTCAATGGCACCAAACTCCTCCACTCCCAACTCCAGTGTAAAAGAAGGGCCTCGCAGCCAAGAGGAGGGGCATTGCAAGTTTCAGCATCGAGTGCAAAGAAGATTCTTATAATGGGAGGGACCCGATTCATCGGCGTCTTCTTGTCAAGACTCCTTGTTAAAGAGGGTCATCAGGTGACTCTGTTTACCAGAGGAAAAGCACCCATTACTCAGCAGTTGCCAGGAGAATCCGATGCGGATTACACAGATTTCGCTTCAAAGATTTTGCACTTGAAAGGAGACAGAAGGGACTATGACTTTGTGAAATCCAGTCTTTCAGCTGAAGGCTATGACGTTGTTTACGATATAAATGGACGAGAGGCAGAAGAAGTTGTGCCGATAATTGAGGGACTACCGAAGATAGAACAGTACATATACTGCTCTTCAGCCGGTGTCTATCTCAAATCTGATCAGCTACCTCACTTTGAGATCGATGCAGTTGATCCAAAGAGCAGGCACAAGGGAAAGCTCGAGACAGAGAGCTTGCTCGAATCAAAGGGTGTTAACTGGACTTCAATAAGGCCAGTCTACATCTATGGACCATTGAACTATAACCCTGTTGAAGAGTGGTTCTTCCACCGGTTGAAAGCTGGCCGCCCAATCCCAGTTCCGAACTCAGGGATACAAATTACACAGCTTGGTCATGTCAAGGACTTAGCGACTGCCTTCATTAAGGTTCTTGGTAACGAAAAGGCCAGCAAACAAGTATTCAACATCTCGGGAGACAAATATGTAACCTTTGACGGACTAGCAAAAGCATGCGCAAAGGCTGCTGGTTTTCCTGAGCCTGAGATCATTCATTACAACCCCAAAGAGTTCGATTTTGGCAAGAAGAAGGCCTTTCCATTCCGTGACCAGCATTTCTTTGCATCGATCGACAAAGCAAAGAGCGTGCTCGGATGGAAACCCGAATATGACCTGGTGGAAGGTCTTGCGGACTCGTACAACCTAGACTTTGGGAGAGGAACATTCAGGAAAGCAGCTGATTTCTCAACAGATGACATCATTCTTGGCAAAAGTCTTGTCCTCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000427 GO:0000428 GO:0001101 GO:0001871 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005777 GO:0005840 GO:0005911 GO:0005975 GO:0005996 GO:0006139 GO:0006355 GO:0006364 GO:0006396 GO:0006412 GO:0006417 GO:0006518 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007623 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009657 GO:0009658 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0009991 GO:0010035 GO:0010287 GO:0010297 GO:0010319 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0019538 GO:0022613 GO:0030054 GO:0030246 GO:0030247 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031668 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0032544 GO:0032991 GO:0033554 GO:0034248 GO:0034250 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042254 GO:0042579 GO:0042631 GO:0042742 GO:0043043 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0045893 GO:0045935 GO:0046483 GO:0048046 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:1990904 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

384

Amino Acids

42.61

Weight (kDa)

8.93

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 61 - 278 4.6e-16 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 65 - 217 2.5e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
Acc16I TGCGCA 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 1 cut(s) 645
AciI CCGC 4 cut(s) 26, 314, 712, 1052
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 1 cut(s) 709
AcuI CTGAAG 2 cut(s) 420, 486
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 488, 1061
AfiI CCNNNNNNNGG 2 cut(s) 143, 734
AgeI ACCGGT 1 cut(s) 696
AgsI TTSAA 8 cut(s) 82, 336, 352, 633, 664, 680, 703, 829
AjnI CCWGG 2 cut(s) 298, 1035
AluBI AGCT 8 cut(s) 398, 532, 586, 600, 707, 754, 1100, 1149
AluI AGCT 8 cut(s) 398, 532, 586, 600, 707, 754, 1100, 1149
Alw21I GWGCWC 1 cut(s) 1014
Alw26I GTCTC 3 cut(s) 351, 584, 834
AlwI GGATC 1 cut(s) 554
Ama87I CYCGRG 2 cut(s) 587, 835
AoxI GGCC 5 cut(s) 126, 638, 709, 810, 954
ApeKI GCWGC 4 cut(s) 28, 133, 887, 1097
ArsI GACNNNNNNTTYG 3 cut(s) 359, 391, 1125
AsiGI ACCGGT 1 cut(s) 696
AspLEI GCGC 1 cut(s) 881
AspS9I GGNCC 3 cut(s) 126, 198, 656
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 2 cut(s) 587, 835
AvaII GGWCC 2 cut(s) 198, 656
BanI GGYRCC 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 1014
BbvCI CCTCAGC 1 cut(s) 1145
BbvI GCAGC 4 cut(s) 40, 145, 874, 1109
BccI CCATC 2 cut(s) 49, 1011
BciT130I CCWGG 2 cut(s) 300, 1037
BciVI GTATCC 1 cut(s) 732
BclI TGATCA 1 cut(s) 526
BcoDI GTCTC 3 cut(s) 351, 584, 834
BfaI CTAG 2 cut(s) 866, 1067
BfuI GTATCC 1 cut(s) 732
BisI GCNGC 5 cut(s) 29, 134, 712, 888, 1098
BlsI GCNGC 5 cut(s) 30, 135, 713, 889, 1099
Bme1390I CCNGG 2 cut(s) 300, 1037
Bme18I GGWCC 2 cut(s) 198, 656
BmeT110I CYCGRG 2 cut(s) 587, 835
BmgT120I GGNCC 3 cut(s) 126, 198, 656
BmiI GGNNCC 3 cut(s) 88, 199, 200
BmrFI CCNGG 2 cut(s) 300, 1037
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 4 cut(s) 173, 301, 541, 995
BmuI ACTGGG 1 cut(s) 716
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 1 cut(s) 95
Bpu10I CCTNAGC 2 cut(s) 900, 1145
Bsa29I ATCGAT 2 cut(s) 549, 989
BsaHI GRCGYC 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 696
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 734
Bse118I RCCGGY 2 cut(s) 506, 696
Bse1I ACTGG 5 cut(s) 112, 387, 629, 641, 722
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 2 cut(s) 300, 1037
BseCI ATCGAT 2 cut(s) 549, 989
BseGI GGATG 1 cut(s) 1022
BseLI CCNNNNNNNGG 2 cut(s) 143, 734
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 4 cut(s) 302, 747, 891, 897
BseNI ACTGG 5 cut(s) 112, 387, 629, 641, 722
BseRI GAGGAG 3 cut(s) 44, 86, 156
BseXI GCAGC 4 cut(s) 40, 145, 874, 1109
Bsh1285I CGRYCG 1 cut(s) 993
BshFI GGCC 5 cut(s) 128, 640, 711, 812, 956
BshNI GGYRCC 1 cut(s) 86
BshTI ACCGGT 1 cut(s) 696
BshVI ATCGAT 2 cut(s) 549, 989
BsiEI CGRYCG 1 cut(s) 993
BsiHKAI GWGCWC 1 cut(s) 1014
BsiHKCI CYCGRG 2 cut(s) 587, 835
BsiSI CCGG 2 cut(s) 507, 697
BslFI GGGAC 3 cut(s) 211, 380, 480
BslI CCNNNNNNNGG 2 cut(s) 143, 734
BsmAI GTCTC 3 cut(s) 351, 584, 834
BsmFI GGGAC 3 cut(s) 211, 380, 480
BsnI GGCC 5 cut(s) 128, 640, 711, 812, 956
BsoBI CYCGRG 2 cut(s) 587, 835
Bsp1286I GDGCHC 1 cut(s) 1014
Bsp143I GATC 5 cut(s) 526, 546, 559, 909, 990
BspACI CCGC 4 cut(s) 26, 314, 712, 1052
BspANI GGCC 5 cut(s) 128, 640, 711, 812, 956
BspCNI CTCAG 4 cut(s) 301, 746, 892, 898
BspDI ATCGAT 2 cut(s) 549, 989
BspLI GGNNCC 3 cut(s) 88, 199, 200
BspPI GGATC 1 cut(s) 554
BspQI GCTCTTC 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 149
BsrFI RCCGGY 2 cut(s) 506, 696
BsrI ACTGG 5 cut(s) 112, 387, 629, 641, 722
BssAI RCCGGY 2 cut(s) 506, 696
BssMI GATC 5 cut(s) 526, 546, 559, 909, 990
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 2 cut(s) 300, 1037
Bst4CI ACNGT 1 cut(s) 486
Bst6I CTCTTC 2 cut(s) 505, 675
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 2 cut(s) 170, 887
BstC8I GCNNGC 6 cut(s) 572, 602, 709, 814, 877, 1010
BstDEI CTNAG 6 cut(s) 288, 733, 772, 900, 906, 1145
BstF5I GGATG 1 cut(s) 1022
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 5 cut(s) 529, 549, 562, 912, 993
BstMAI GTCTC 3 cut(s) 351, 584, 834
BstMBI GATC 5 cut(s) 526, 546, 559, 909, 990
BstMCI CGRYCG 1 cut(s) 993
BstMWI GCNNNNNNNGC 3 cut(s) 28, 170, 887
BstNI CCWGG 2 cut(s) 300, 1037
BstNSI RCATGY 1 cut(s) 879
BstSCI CCNGG 2 cut(s) 298, 1035
BstV1I GCAGC 4 cut(s) 40, 145, 874, 1109
BstV2I GAAGAC 1 cut(s) 208
Bsu15I ATCGAT 2 cut(s) 549, 989
BsuI GTATCC 1 cut(s) 732
BsuRI GGCC 5 cut(s) 128, 640, 711, 812, 956
BsuTUI ATCGAT 2 cut(s) 549, 989
BtsCI GGATG 1 cut(s) 1022
BtsIMutI CAGTG 1 cut(s) 119
Cac8I GCNNGC 6 cut(s) 572, 602, 709, 814, 877, 1010
CfoI GCGC 1 cut(s) 881
Cfr10I RCCGGY 2 cut(s) 506, 696
Cfr13I GGNCC 3 cut(s) 126, 198, 656
ClaI ATCGAT 2 cut(s) 549, 989
CseI GACGC 1 cut(s) 202
CsiI ACCWGGT 1 cut(s) 1035
Csp6I GTAC 2 cut(s) 487, 1060
CspAI ACCGGT 1 cut(s) 696
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 2 cut(s) 761, 876
CviQI GTAC 2 cut(s) 487, 1060
DdeI CTNAG 6 cut(s) 288, 733, 772, 900, 906, 1145
DpnI GATC 5 cut(s) 528, 548, 561, 911, 992
DpnII GATC 5 cut(s) 526, 546, 559, 909, 990
EaeI YGGCCR 1 cut(s) 709
Eam1104I CTCTTC 2 cut(s) 505, 675
EarI CTCTTC 2 cut(s) 505, 675
Eco147I AGGCCT 1 cut(s) 956
Eco47I GGWCC 2 cut(s) 198, 656
Eco57I CTGAAG 2 cut(s) 420, 486
Eco88I CYCGRG 2 cut(s) 587, 835
EcoO109I RGGNCCY 2 cut(s) 126, 198
EcoRII CCWGG 2 cut(s) 298, 1035
FaeI CATG 2 cut(s) 764, 879
FalI AAGNNNNNCTT 2 cut(s) 941, 973
FaqI GGGAC 3 cut(s) 211, 380, 480
FatI CATG 2 cut(s) 760, 875
FbaI TGATCA 1 cut(s) 526
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 5 cut(s) 29, 134, 712, 888, 1098
FokI GGATG 1 cut(s) 1029
Fsp4HI GCNGC 5 cut(s) 29, 134, 712, 888, 1098
FspBI CTAG 2 cut(s) 866, 1067
FspI TGCGCA 1 cut(s) 880
GlaI GCGC 1 cut(s) 880
GluI GCNGC 5 cut(s) 29, 134, 712, 888, 1098
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 5 cut(s) 128, 640, 711, 812, 956
HapII CCGG 2 cut(s) 507, 697
HgaI GACGC 1 cut(s) 202
HhaI GCGC 1 cut(s) 881
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 2 cut(s) 764, 879
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HinfI GANTC 7 cut(s) 181, 204, 228, 256, 305, 608, 1055
HpaI GTTAAC 1 cut(s) 622
HpaII CCGG 2 cut(s) 507, 697
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 4 cut(s) 264, 418, 622, 646
Hpy188I TCNGA 5 cut(s) 76, 310, 526, 729, 1016
Hpy188III TCNNGA 5 cut(s) 225, 589, 837, 899, 1090
Hpy8I GTNNAC 4 cut(s) 264, 418, 622, 646
HpyAV CCTTC 7 cut(s) 72, 117, 357, 395, 793, 946, 1037
HpyCH4III ACNGT 1 cut(s) 486
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 6 cut(s) 22, 154, 173, 346, 554, 986
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 170, 887
HpyF3I CTNAG 6 cut(s) 288, 733, 772, 900, 906, 1145
HpySE526I ACGT 1 cut(s) 411
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 2 cut(s) 764, 879
HspAI GCGC 1 cut(s) 879
KflI GGGWCCC 1 cut(s) 198
Ksp22I TGATCA 1 cut(s) 526
KspAI GTTAAC 1 cut(s) 622
Kzo9I GATC 5 cut(s) 526, 546, 559, 909, 990
LguI GCTCTTC 1 cut(s) 505
Lsp1109I GCAGC 4 cut(s) 40, 145, 874, 1109
LweI GCATC 4 cut(s) 173, 301, 541, 995
MabI ACCWGGT 1 cut(s) 1035
MaeI CTAG 2 cut(s) 866, 1067
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 4 cut(s) 253, 800, 850, 968
MalI GATC 5 cut(s) 528, 548, 561, 911, 992
MboI GATC 5 cut(s) 526, 546, 559, 909, 990
MboII GAAGA 8 cut(s) 190, 208, 455, 487, 492, 682, 692, 961
MhlI GDGCHC 1 cut(s) 1014
MluCI AATT 2 cut(s) 459, 744
MlyI GAGTC 3 cut(s) 222, 250, 1049
MseI TTAA 3 cut(s) 237, 621, 789
MspA1I CMGCKG 2 cut(s) 398, 1100
MspI CCGG 2 cut(s) 507, 697
MspR9I CCNGG 2 cut(s) 300, 1037
MvaI CCWGG 2 cut(s) 300, 1037
MwoI GCNNNNNNNGC 3 cut(s) 28, 170, 887
NdeII GATC 5 cut(s) 526, 546, 559, 909, 990
NlaIII CATG 2 cut(s) 764, 879
NlaIV GGNNCC 3 cut(s) 88, 199, 200
NmuCI GTSAC 2 cut(s) 253, 968
NsbI TGCGCA 1 cut(s) 880
NspI RCATGY 1 cut(s) 879
PaeI GCATGC 1 cut(s) 879
PaeR7I CTCGAG 1 cut(s) 587
PceI AGGCCT 1 cut(s) 956
PciSI GCTCTTC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PfeI GAWTC 4 cut(s) 181, 204, 305, 608
PinAI ACCGGT 1 cut(s) 696
PkrI GCNGC 5 cut(s) 30, 135, 713, 889, 1099
Ple19I CGATCG 1 cut(s) 993
PleI GAGTC 3 cut(s) 222, 250, 1049
PpsI GAGTC 3 cut(s) 222, 250, 1049
PpuMI RGGWCCY 1 cut(s) 198
PsiI TTATAA 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 298, 1035
PspGI CCWGG 2 cut(s) 298, 1035
PspN4I GGNNCC 3 cut(s) 88, 199, 200
PspPI GGNCC 3 cut(s) 126, 198, 656
PspPPI RGGWCCY 1 cut(s) 198
PvuI CGATCG 1 cut(s) 993
PvuII CAGCTG 2 cut(s) 398, 1100
RsaI GTAC 2 cut(s) 488, 1061
RsaNI GTAC 2 cut(s) 487, 1060
SapI GCTCTTC 1 cut(s) 505
SaqAI TTAA 3 cut(s) 237, 621, 789
SatI GCNGC 5 cut(s) 29, 134, 712, 888, 1098
Sau3AI GATC 5 cut(s) 526, 546, 559, 909, 990
Sau96I GGNCC 3 cut(s) 126, 198, 656
SchI GAGTC 3 cut(s) 222, 250, 1049
ScrFI CCNGG 2 cut(s) 300, 1037
SduI GDGCHC 1 cut(s) 1014
SexAI ACCWGGT 1 cut(s) 1035
SfaNI GCATC 4 cut(s) 173, 301, 541, 995
Sfr274I CTCGAG 1 cut(s) 587
SinI GGWCC 2 cut(s) 198, 656
SlaI CTCGAG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
SphI GCATGC 1 cut(s) 879
Sse9I AATT 2 cut(s) 459, 744
SseBI AGGCCT 1 cut(s) 956
SsiI CCGC 4 cut(s) 26, 314, 712, 1052
SspMI CTAG 2 cut(s) 866, 1067
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 2 cut(s) 298, 1035
TaaI ACNGT 1 cut(s) 486
TaiI ACGT 1 cut(s) 414
TaqI TCGA 7 cut(s) 167, 549, 588, 606, 936, 989, 993
TasI AATT 2 cut(s) 459, 744
TatI WGTACW 1 cut(s) 486
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 4 cut(s) 181, 204, 305, 608
Tru1I TTAA 3 cut(s) 237, 621, 789
Tru9I TTAA 3 cut(s) 237, 621, 789
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 2 cut(s) 253, 968
TseI GCWGC 4 cut(s) 28, 133, 887, 1097
Tsp45I GTSAC 2 cut(s) 253, 968
TspDTI ATGAA 3 cut(s) 196, 775, 905
TspGWI ACGGA 2 cut(s) 876, 956
TspRI CASTG 1 cut(s) 119
VpaK11BI GGWCC 2 cut(s) 198, 656
XceI RCATGY 1 cut(s) 879
XhoI CTCGAG 1 cut(s) 587
XmiI GTMKAC 1 cut(s) 645
XspI CTAG 2 cut(s) 866, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.