Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007101

Physical Location & Seq

Genomic Coordinates Forward (+)

30235188 .. 30239082

Gene Structure 3895 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1226600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGCGAATGGTGAAGTGCAAGGACCTTATTGTTCATGGGGAGCTGGAAAGGAAACCGAAAACTTGTCACTGAAGGTCCCAAATGAGATTTCTAAGGTGAAACAGAGAAAACAGAAAGTTCTACTTACTTCTGCTTCTGCCCAAGATTTGAGGTATAACGACATAGAAAAAGAGAAGGAGGATGTCCAAACTCCTAGAGGAGTTTTAGAAGCTTGCGTGAGAGGCTTTGAATCCGAGACTAGTACTTCCGAAAACAGCACGACTGAAAATGGCACGAGTGAGTCATCAGTGCGTTGTTCAAGTTCGAATTCACTTTCTCACTGGCGCAAGTTCTTTAAACAATGGAGTAAAAGATCGCTTAAGCGCTTAGCCTCCTTCCCTCCGCTTGGTGTGCCGAAGATATCATCACGAAGAAGAATCAGGAGTGCAAGAGAGGATCCAGCATTAACTAATATATACAACTTCAAGTCTTCGTTGGTGAACTTTACCAGCTCTGAGCTCCAAAAGGCGACCGATAATTTTAACAGTGAAAACCAAATTGGAAAGGGTGGTTATTCAGAAGTTTACAGGGGTTGTTTGAAAAGTGGGCAGCTAATAGCAGTCAAGCGGCTGACGAAAGGAACAGCAGATGAGAAAACAGCAGGCTTTCTATCTGAACTTGGCATTATAGCTCATGTTGATCATCCTAATGCCGCTAAGTTAATTGGATGTTGCGTTGAAGGAGGAATGTACCTCGTTTTTGAATTATCTTCACTTGGGAGTTTAGGATCGCTCCTGCACAGTCCGAAGGCTAACAAACTTGATTGGAGTAAAAGATACAAAATTGCTTTGGGGACAGCAGATGGTCTGCTATACCTTCATGAGAGTTGCCAAAGGCGAATCATTCATAGAGATATCAAAGCCGATAATATTCTGCTCACAGAGGATTTTGTGCCTCAGATATGTGATTTCGGGCTTGCAAAGTGGCTCCCCAAACAATGGACTCATCACAATGTCTCTAAATCTGAAGGCACATTCGGGTATTTTGCTCCCGAGTATTTCATGCATGGAATAGTAGATGAAAAAACCGATGTTTATTCTTTTGGAGTCCTACTCTTGGAGCTTATAACCGGGCGTCCAGCTTTGGATGATCTTCAGAACAGCCTTGTTCTTTGGGCAAAGCCTTTGCTTGATAACAATGAGATCAAGGAGCTTGTTGATCCTAATATTGGCGACAACTATGACCAGAAAGAGATGGATCACATGATTTTGACAGCGGCTTTATGCATCGAGCAGTCTTCTATTCTACGTCCTCGCATGAGTCAGGTTGTGGTGCTGCTGAGAGGCGATGAATACGTATCCAAATGTGCAAAAGAGTCGAAAAGGAGGTCCATACAAAGAACATACTCGGAAGAACTACTGGATGCACAAGAGTACAATTCAACAAAGTATCTGGGCGATCTCAACCGACACAAGCAGGTCGCTTTTGGTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

492

Amino Acids

55.1

Weight (kDa)

8.36

Isoelectric Point (pI)

47.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	174 - 378	3.8e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	176 - 436	4.6e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007101)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05140 AT3G05140
fragaria_vesca	FvH4_6g04990 FvH4_6g04990 FvH4_6g04990 FvH4_6g04990 FvH4_6g04990 FvH4_6g04990
malus_domestica	MD04G1211700.v1.1 MD12G1226600.v1.1
prunus_persica	Prupe.6G332300_v2.0.a1 Prupe.6G332300_v2.0.a1 Prupe.6G332300_v2.0.a1
pyrus_communis	pycom04g18770 pycom12g21110
rosa_chinensis	RchiOBHm_Chr3g0453501
rosa_laevigata	RLG00000025508
rosa_multiflora	Rmu_sc0000510.1_g000014
rosa_roxburghii	Rroxscaffold_6G00430470
rosa_rugosa	Rorug02G0649600 Rorug02G0649600
rosa_samantha	Rh3AG052500 Rh3BG053900 Rh3CG053300 Rh3DG054100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1106
Acc36I	ACCTGC	1 cut(s)	1447
AccB7I	CCANNNNNTGG	1 cut(s)	978
AciI	CCGC	4 cut(s)	383, 607, 693, 1256
AclWI	GGATC	5 cut(s)	431, 444, 775, 1193, 1245
AcsI	RAATTY	1 cut(s)	307
AcuI	CTGAAG	3 cut(s)	92, 1026, 1118
AcyI	GRCGYC	1 cut(s)	1114
AfaI	GTAC	3 cut(s)	244, 731, 1415
AfeI	AGCGCT	1 cut(s)	365
AfiI	CCNNNNNNNGG	4 cut(s)	386, 978, 1096, 1208
AflII	CTTAAG	1 cut(s)	359
AgsI	TTSAA	7 cut(s)	230, 300, 466, 580, 719, 743, 1422
AhlI	ACTAGT	1 cut(s)	239
AjuI	GAANNNNNNNTTGG	4 cut(s)	73, 105, 522, 554
AluBI	AGCT	9 cut(s)	44, 212, 492, 499, 592, 671, 1102, 1121, 1192
AluI	AGCT	9 cut(s)	44, 212, 492, 499, 592, 671, 1102, 1121, 1192
Alw21I	GWGCWC	1 cut(s)	501
Alw26I	GTCTC	2 cut(s)	230, 1000
AlwI	GGATC	5 cut(s)	431, 444, 775, 1193, 1245
Ama87I	CYCGRG	1 cut(s)	1031
Aor51HI	AGCGCT	1 cut(s)	365
ApeKI	GCWGC	2 cut(s)	589, 1315
ApoI	RAATTY	1 cut(s)	307
ArsI	GACNNNNNNTTYG	2 cut(s)	1352, 1384
AspLEI	GCGC	2 cut(s)	327, 366
AspS9I	GGNCC	3 cut(s)	23, 76, 1368
AsuC2I	CCSGG	1 cut(s)	1111
AsuHPI	GGTGA	3 cut(s)	23, 109, 490
AsuII	TTCGAA	1 cut(s)	305
AvaI	CYCGRG	1 cut(s)	1031
AvaII	GGWCC	3 cut(s)	23, 76, 1368
BamHI	GGATCC	1 cut(s)	436
BanII	GRGCYC	1 cut(s)	501
BauI	CACGAG	1 cut(s)	274
BbsI	GAAGAC	2 cut(s)	462, 1269
Bbv12I	GWGCWC	1 cut(s)	501
BbvI	GCAGC	2 cut(s)	601, 1302
BccI	CCATC	2 cut(s)	836, 1228
BcgI	CGANNNNNNTGC	2 cut(s)	1338, 1372
BciVI	GTATCC	1 cut(s)	1348
BclI	TGATCA	1 cut(s)	679
BcnI	CCSGG	1 cut(s)	1111
BcoDI	GTCTC	2 cut(s)	230, 1000
BcuI	ACTAGT	1 cut(s)	239
BfaI	CTAG	2 cut(s)	195, 240
BfoI	RGCGCY	1 cut(s)	367
BfrI	CTTAAG	1 cut(s)	359
BfuAI	ACCTGC	1 cut(s)	1447
BfuI	GTATCC	1 cut(s)	1348
BisI	GCNGC	5 cut(s)	590, 608, 693, 1257, 1316
BlpI	GCTNAGC	1 cut(s)	367
BlsI	GCNGC	5 cut(s)	591, 609, 694, 1258, 1317
BmcAI	AGTACT	1 cut(s)	244
Bme1390I	CCNGG	1 cut(s)	1111
Bme18I	GGWCC	3 cut(s)	23, 76, 1368
BmeT110I	CYCGRG	1 cut(s)	1031
BmgT120I	GGNCC	3 cut(s)	23, 76, 1368
BmiI	GGNNCC	3 cut(s)	78, 438, 968
BmrFI	CCNGG	1 cut(s)	1111
BmsI	GCATC	2 cut(s)	1275, 1393
BpiI	GAAGAC	2 cut(s)	462, 1269
BplI	GAGNNNNNCTC	2 cut(s)	1077, 1109
Bpu1102I	GCTNAGC	1 cut(s)	367
Bpu14I	TTCGAA	1 cut(s)	305
BpuMI	CCSGG	1 cut(s)	1111
BsaAI	YACGTR	1 cut(s)	1336
BsaHI	GRCGYC	1 cut(s)	1114
Bsc4I	CCNNNNNNNGG	4 cut(s)	386, 978, 1096, 1208
Bse1I	ACTGG	2 cut(s)	326, 1404
BseGI	GGATG	5 cut(s)	187, 682, 713, 1132, 1408
BseLI	CCNNNNNNNGG	4 cut(s)	386, 978, 1096, 1208
BseMII	CTCAG	3 cut(s)	486, 950, 1310
BseNI	ACTGG	2 cut(s)	326, 1404
BseRI	GAGGAG	1 cut(s)	213
BseXI	GCAGC	2 cut(s)	601, 1302
BsgI	GTGCAG	1 cut(s)	761
Bsh1285I	CGRYCG	1 cut(s)	513
BsiEI	CGRYCG	1 cut(s)	513
BsiHKAI	GWGCWC	1 cut(s)	501
BsiHKCI	CYCGRG	1 cut(s)	1031
BsiSI	CCGG	1 cut(s)	1110
BslFI	GGGAC	2 cut(s)	62, 847
BslI	CCNNNNNNNGG	4 cut(s)	386, 978, 1096, 1208
BsmAI	GTCTC	2 cut(s)	230, 1000
BsmFI	GGGAC	2 cut(s)	62, 847
BsoBI	CYCGRG	1 cut(s)	1031
Bsp119I	TTCGAA	1 cut(s)	305
Bsp1286I	GDGCHC	1 cut(s)	501
Bsp143I	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
Bsp1720I	GCTNAGC	1 cut(s)	367
BspACI	CCGC	4 cut(s)	383, 607, 693, 1256
BspCNI	CTCAG	3 cut(s)	487, 949, 1311
BspHI	TCATGA	1 cut(s)	859
BspLI	GGNNCC	3 cut(s)	78, 438, 968
BspMI	ACCTGC	1 cut(s)	1447
BspPI	GGATC	5 cut(s)	431, 444, 775, 1193, 1245
BspT104I	TTCGAA	1 cut(s)	305
BspTI	CTTAAG	1 cut(s)	359
BsrI	ACTGG	2 cut(s)	326, 1404
BssMI	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
BssNI	GRCGYC	1 cut(s)	1114
BssSI	CACGAG	1 cut(s)	274
Bst2BI	CACGAG	1 cut(s)	274
Bst4CI	ACNGT	2 cut(s)	527, 782
BstACI	GRCGYC	1 cut(s)	1114
BstAFI	CTTAAG	1 cut(s)	359
BstBAI	YACGTR	1 cut(s)	1336
BstBI	TTCGAA	1 cut(s)	305
BstC8I	GCNNGC	3 cut(s)	214, 643, 957
BstDEI	CTNAG	6 cut(s)	93, 367, 495, 696, 936, 1319
BstF5I	GGATG	5 cut(s)	187, 682, 713, 1132, 1408
BstH2I	RGCGCY	1 cut(s)	367
BstHHI	GCGC	2 cut(s)	327, 366
BstKTI	GATC	9 cut(s)	356, 439, 682, 770, 1132, 1185, 1201, 1240, 1441
BstMAI	GTCTC	2 cut(s)	230, 1000
BstMBI	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
BstMCI	CGRYCG	1 cut(s)	513
BstMWI	GCNNNNNNNGC	2 cut(s)	222, 391
BstSCI	CCNGG	1 cut(s)	1109
BstSNI	TACGTA	1 cut(s)	1336
BstV1I	GCAGC	2 cut(s)	601, 1302
BstV2I	GAAGAC	2 cut(s)	462, 1269
BstX2I	RGATCY	1 cut(s)	436
BstYI	RGATCY	1 cut(s)	436
BsuI	GTATCC	1 cut(s)	1348
BtgZI	GCGATG	1 cut(s)	1341
BtsCI	GGATG	5 cut(s)	187, 682, 713, 1132, 1408
BtsIMutI	CAGTG	4 cut(s)	68, 294, 319, 532
BveI	ACCTGC	1 cut(s)	1447
Cac8I	GCNNGC	3 cut(s)	214, 643, 957
CciI	TCATGA	1 cut(s)	859
CfoI	GCGC	2 cut(s)	327, 366
Cfr13I	GGNCC	3 cut(s)	23, 76, 1368
CseI	GACGC	1 cut(s)	1103
Csp6I	GTAC	3 cut(s)	243, 730, 1414
CviAII	CATG	7 cut(s)	36, 674, 860, 1042, 1046, 1243, 1297
CviQI	GTAC	3 cut(s)	243, 730, 1414
DdeI	CTNAG	6 cut(s)	93, 367, 495, 696, 936, 1319
DpnI	GATC	9 cut(s)	355, 438, 681, 769, 1131, 1184, 1200, 1239, 1440
DpnII	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
DraI	TTTAAA	1 cut(s)	337
Ecl136II	GAGCTC	1 cut(s)	499
Eco105I	TACGTA	1 cut(s)	1336
Eco24I	GRGCYC	1 cut(s)	501
Eco32I	GATATC	2 cut(s)	402, 895
Eco47I	GGWCC	3 cut(s)	23, 76, 1368
Eco47III	AGCGCT	1 cut(s)	365
Eco53kI	GAGCTC	1 cut(s)	499
Eco57I	CTGAAG	3 cut(s)	92, 1026, 1118
Eco88I	CYCGRG	1 cut(s)	1031
EcoICRI	GAGCTC	1 cut(s)	499
EcoO109I	RGGNCCY	2 cut(s)	23, 76
EcoRI	GAATTC	1 cut(s)	307
EcoRV	GATATC	2 cut(s)	402, 895
EcoT22I	ATGCAT	2 cut(s)	1047, 1268
EcoT38I	GRGCYC	1 cut(s)	501
FaeI	CATG	7 cut(s)	39, 677, 863, 1045, 1049, 1246, 1300
FalI	AAGNNNNNCTT	2 cut(s)	108, 140
FaqI	GGGAC	2 cut(s)	62, 847
FatI	CATG	7 cut(s)	35, 673, 859, 1041, 1045, 1242, 1296
FbaI	TGATCA	1 cut(s)	679
Fnu4HI	GCNGC	5 cut(s)	590, 608, 693, 1257, 1316
FokI	GGATG	5 cut(s)	194, 669, 720, 1139, 1415
FriOI	GRGCYC	1 cut(s)	501
Fsp4HI	GCNGC	5 cut(s)	590, 608, 693, 1257, 1316
FspBI	CTAG	2 cut(s)	195, 240
GlaI	GCGC	2 cut(s)	326, 365
GluI	GCNGC	5 cut(s)	590, 608, 693, 1257, 1316
HaeII	RGCGCY	1 cut(s)	367
HapII	CCGG	1 cut(s)	1110
HgaI	GACGC	1 cut(s)	1103
HhaI	GCGC	2 cut(s)	327, 366
Hin1I	GRCGYC	1 cut(s)	1114
Hin1II	CATG	7 cut(s)	39, 677, 863, 1045, 1049, 1246, 1300
Hin6I	GCGC	2 cut(s)	325, 364
HinP1I	GCGC	2 cut(s)	325, 364
HindIII	AAGCTT	1 cut(s)	210
HinfI	GANTC	8 cut(s)	230, 281, 417, 879, 982, 1086, 1300, 1355
HpaII	CCGG	1 cut(s)	1110
HphI	GGTGA	3 cut(s)	23, 109, 490
Hpy166II	GTNNAC	2 cut(s)	481, 565
Hpy188III	TCNNGA	5 cut(s)	408, 421, 860, 1031, 1473
Hpy8I	GTNNAC	2 cut(s)	481, 565
HpyAV	CCTTC	7 cut(s)	67, 169, 385, 713, 781, 866, 1001
HpyCH4III	ACNGT	2 cut(s)	527, 782
HpyCH4IV	ACGT	2 cut(s)	1288, 1335
HpyCH4V	TGCA	8 cut(s)	19, 428, 778, 959, 1045, 1266, 1349, 1406
HpyF10VI	GCNNNNNNNGC	2 cut(s)	222, 391
HpyF3I	CTNAG	6 cut(s)	93, 367, 495, 696, 936, 1319
HpySE526I	ACGT	2 cut(s)	1288, 1335
Hsp92I	GRCGYC	1 cut(s)	1114
Hsp92II	CATG	7 cut(s)	39, 677, 863, 1045, 1049, 1246, 1300
HspAI	GCGC	2 cut(s)	325, 364
Ksp22I	TGATCA	1 cut(s)	679
Kzo9I	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
LmnI	GCTCC	7 cut(s)	41, 504, 777, 972, 1033, 1099, 1189
Lsp1109I	GCAGC	2 cut(s)	601, 1302
LweI	GCATC	2 cut(s)	1275, 1393
MaeI	CTAG	2 cut(s)	195, 240
MaeII	ACGT	2 cut(s)	1288, 1335
MaeIII	GTNAC	1 cut(s)	66
MalI	GATC	9 cut(s)	355, 438, 681, 769, 1131, 1184, 1200, 1239, 1440
MboI	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
MboII	GAAGA	8 cut(s)	409, 423, 426, 462, 741, 1124, 1269, 1403
MflI	RGATCY	1 cut(s)	436
MhlI	GDGCHC	1 cut(s)	501
MluCI	AATT	7 cut(s)	307, 517, 537, 702, 743, 822, 1417
MlyI	GAGTC	5 cut(s)	290, 976, 1095, 1309, 1364
Mph1103I	ATGCAT	2 cut(s)	1047, 1268
MseI	TTAA	5 cut(s)	336, 360, 446, 522, 701
MslI	CAYNNNNRTG	2 cut(s)	687, 990
MspA1I	CMGCKG	1 cut(s)	1256
MspCI	CTTAAG	1 cut(s)	359
MspI	CCGG	1 cut(s)	1110
MspR9I	CCNGG	1 cut(s)	1111
MwoI	GCNNNNNNNGC	2 cut(s)	222, 391
NciI	CCSGG	1 cut(s)	1111
NdeII	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
NlaIII	CATG	7 cut(s)	39, 677, 863, 1045, 1049, 1246, 1300
NlaIV	GGNNCC	3 cut(s)	78, 438, 968
NmuCI	GTSAC	1 cut(s)	66
NsiI	ATGCAT	2 cut(s)	1047, 1268
NspV	TTCGAA	1 cut(s)	305
PagI	TCATGA	1 cut(s)	859
PfeI	GAWTC	3 cut(s)	230, 417, 879
PflMI	CCANNNNNTGG	1 cut(s)	978
PkrI	GCNGC	5 cut(s)	591, 609, 694, 1258, 1317
PleI	GAGTC	5 cut(s)	289, 976, 1094, 1308, 1363
PpsI	GAGTC	5 cut(s)	289, 976, 1094, 1308, 1363
Ppu21I	YACGTR	1 cut(s)	1336
PpuMI	RGGWCCY	2 cut(s)	23, 76
PsiI	TTATAA	1 cut(s)	1106
Psp124BI	GAGCTC	1 cut(s)	501
Psp5II	RGGWCCY	2 cut(s)	23, 76
PspN4I	GGNNCC	3 cut(s)	78, 438, 968
PspPI	GGNCC	3 cut(s)	23, 76, 1368
PspPPI	RGGWCCY	2 cut(s)	23, 76
PsuI	RGATCY	1 cut(s)	436
RsaI	GTAC	3 cut(s)	244, 731, 1415
RsaNI	GTAC	3 cut(s)	243, 730, 1414
RseI	CAYNNNNRTG	2 cut(s)	687, 990
SacI	GAGCTC	1 cut(s)	501
SaqAI	TTAA	5 cut(s)	336, 360, 446, 522, 701
SatI	GCNGC	5 cut(s)	590, 608, 693, 1257, 1316
Sau3AI	GATC	9 cut(s)	353, 436, 679, 767, 1129, 1182, 1198, 1237, 1438
Sau96I	GGNCC	3 cut(s)	23, 76, 1368
ScaI	AGTACT	1 cut(s)	244
SchI	GAGTC	5 cut(s)	290, 976, 1095, 1309, 1364
ScrFI	CCNGG	1 cut(s)	1111
SduI	GDGCHC	1 cut(s)	501
SfaNI	GCATC	2 cut(s)	1275, 1393
SfuI	TTCGAA	1 cut(s)	305
SinI	GGWCC	3 cut(s)	23, 76, 1368
SmiMI	CAYNNNNRTG	2 cut(s)	687, 990
SmlI	CTYRAG	1 cut(s)	359
SmoI	CTYRAG	1 cut(s)	359
SnaBI	TACGTA	1 cut(s)	1336
SpeI	ACTAGT	1 cut(s)	239
Sse9I	AATT	7 cut(s)	307, 517, 537, 702, 743, 822, 1417
SsiI	CCGC	4 cut(s)	383, 607, 693, 1256
SspI	AATATT	2 cut(s)	910, 1207
SspMI	CTAG	2 cut(s)	195, 240
SstI	GAGCTC	1 cut(s)	501
StyD4I	CCNGG	1 cut(s)	1109
TaaI	ACNGT	2 cut(s)	527, 782
TaiI	ACGT	2 cut(s)	1291, 1338
TaqI	TCGA	3 cut(s)	305, 1269, 1358
TaqII	GACCGA	1 cut(s)	527
TasI	AATT	7 cut(s)	307, 517, 537, 702, 743, 822, 1417
TatI	WGTACW	2 cut(s)	242, 1413
TauI	GCSGC	3 cut(s)	610, 695, 1259
TfiI	GAWTC	3 cut(s)	230, 417, 879
Tru1I	TTAA	5 cut(s)	336, 360, 446, 522, 701
Tru9I	TTAA	5 cut(s)	336, 360, 446, 522, 701
TscAI	CASTG	4 cut(s)	75, 294, 326, 532
TseFI	GTSAC	1 cut(s)	66
TseI	GCWGC	2 cut(s)	589, 1315
Tsp45I	GTSAC	1 cut(s)	66
TspDTI	ATGAA	6 cut(s)	24, 848, 875, 1030, 1074, 1344
TspRI	CASTG	4 cut(s)	75, 294, 326, 532
Van91I	CCANNNNNTGG	1 cut(s)	978
Vha464I	CTTAAG	1 cut(s)	359
VpaK11BI	GGWCC	3 cut(s)	23, 76, 1368
XapI	RAATTY	1 cut(s)	307
XspI	CTAG	2 cut(s)	195, 240
ZrmI	AGTACT	1 cut(s)	244
Zsp2I	ATGCAT	2 cut(s)	1047, 1268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.