pycom12g21110

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007101
Physical Location & Seq
Forward (+)
22365309 .. 22367959
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21110.2

Sequence Viewer

Length: 1173 bp
ATGGGTTCTATGGACTTTCAGTCTGTGTGCAAGCAATGGGGAGCTGGAAAGGAAACCGAAAACTTGTCACTGAAGGTCCCAAATGAGATTTCTAAGGTGAAAGAGAGAAAGCAGAAAGTTCTACTTACTTCTGCTTCAGCCCAAGATTTGAGGTATAACGACATAGAAAAAGAGAAAGAGGATGTCCAAACTCCTAGAGGAGTTTTAGAAGCTTGCGTGAGAGGCTTTGAATCCGAGACTAGTACTTCTGAAAACAGCACAACTGAAAACGGCACGACTGAGTCATCAGTATGTTGTTCAAGTTCGAATTCAGTTTCTCACTGGCGTAAGTTCTTTAAACAATGGGGTAAAAGATCGCTTAAGCGGTTAGCCTCCTTCCCTCCCCTTGGTGTGCCGAAGATATCATCACGAAGAAGAATCAGGAGTGCAAGAGAGAATCCAGCATTAACTAATATATACAACTTCAAGTCTTCGTTGGTGAACTTTACCAGCTCTGAGCTCCAAAAGGCGACCTATAATTTTAACAGTGAAAACCAAATTGGAAAGGGTGGTTATTCAGAAGTTTACAAGGGTTGTTTGAAAAGTGGGCAGCTAGTAGCAGTCAAGCGGCTGACGAAAGGAACAGCAGATGAGAAAACAGCAGGCTTTCTATCTGAACTTGGCATTATAGCTCATGTTGATCATCCTAATGCCGCTAAGTTAATTGGATGTTGCGTTGAAGGAGGAATGTACCTCGTTTTTGAATTATCTTCACTTGGGAGTTTAGGATCGCTTCTGCACAGTCCGAAGGCTAACAAACTTGATTGGAGTAAAAGATACAAAATTGCTTTGGGGACAGCAGATGGTCTGCTATACCTTCATGAGAGTTGCCAGAGGCGAATCATTCATAGAGATATCAAAGCCGATAATATTCTGCTCACAGAGGATTTTGTGCCTCAGATATGTGATTTCGGGCTTGCAAAGTGGCTCCCCAAACAATGGACTCATCACAATGTCTCTAAATCTGAAGGCACATTCGGGTATTTTGCTCCCGAGTATTTCATGCATGGAATAGTAGATGAAAAAACCGATGTTTATTCTTTTGGAGTCCTACTCTTGGAGCTTATAACCGGGCGTCCAGCTTTGGATCGATCTTCAGAACAGCCTTGTACTTTGGGCAAAGCCTTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

391

Amino Acids

43.37

Weight (kDa)

8.93

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AccB7I CCANNNNNTGG 1 cut(s) 978
AciI CCGC 3 cut(s) 364, 607, 693
AclWI GGATC 2 cut(s) 775, 1134
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 4 cut(s) 92, 120, 1026, 1119
AcyI GRCGYC 1 cut(s) 1114
AfaI GTAC 3 cut(s) 244, 731, 1150
AfiI CCNNNNNNNGG 3 cut(s) 386, 978, 1096
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 6 cut(s) 230, 300, 466, 580, 719, 743
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 239
AjuI GAANNNNNNNTTGG 4 cut(s) 73, 105, 522, 554
AluBI AGCT 8 cut(s) 44, 212, 492, 499, 592, 671, 1102, 1121
AluI AGCT 8 cut(s) 44, 212, 492, 499, 592, 671, 1102, 1121
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 2 cut(s) 230, 1000
AlwI GGATC 2 cut(s) 775, 1134
Ama87I CYCGRG 1 cut(s) 1031
ApeKI GCWGC 1 cut(s) 589
ApoI RAATTY 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 76
AsuC2I CCSGG 1 cut(s) 1111
AsuHPI GGTGA 2 cut(s) 109, 490
AsuII TTCGAA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 1031
AvaII GGWCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 1 cut(s) 601
BccI CCATC 1 cut(s) 836
BceAI ACGGC 1 cut(s) 286
BclI TGATCA 1 cut(s) 679
BcnI CCSGG 1 cut(s) 1111
BcoDI GTCTC 2 cut(s) 230, 1000
BcuI ACTAGT 1 cut(s) 239
BfaI CTAG 3 cut(s) 195, 240, 593
BfrI CTTAAG 1 cut(s) 359
BisI GCNGC 3 cut(s) 590, 608, 693
BlsI GCNGC 3 cut(s) 591, 609, 694
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 1111
Bme18I GGWCC 1 cut(s) 76
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 1031
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 2 cut(s) 78, 968
BmrFI CCNGG 1 cut(s) 1111
BpiI GAAGAC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 1077, 1109
Bpu14I TTCGAA 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 1111
Bsa29I ATCGAT 1 cut(s) 1129
BsaHI GRCGYC 1 cut(s) 1114
BsaJI CCNNGG 1 cut(s) 385
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 978, 1096
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 41
BseCI ATCGAT 1 cut(s) 1129
BseDI CCNNGG 1 cut(s) 385
BseGI GGATG 3 cut(s) 187, 682, 713
BseLI CCNNNNNNNGG 3 cut(s) 386, 978, 1096
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 3 cut(s) 270, 486, 950
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 1 cut(s) 601
BsgI GTGCAG 1 cut(s) 761
BshVI ATCGAT 1 cut(s) 1129
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 1031
BsiSI CCGG 1 cut(s) 1110
BslFI GGGAC 2 cut(s) 62, 847
BslI CCNNNNNNNGG 3 cut(s) 386, 978, 1096
BsmAI GTCTC 2 cut(s) 230, 1000
BsmFI GGGAC 2 cut(s) 62, 847
BsoBI CYCGRG 1 cut(s) 1031
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 5 cut(s) 353, 679, 767, 1126, 1130
BspACI CCGC 3 cut(s) 364, 607, 693
BspCNI CTCAG 3 cut(s) 271, 487, 949
BspDI ATCGAT 1 cut(s) 1129
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 2 cut(s) 78, 968
BspPI GGATC 2 cut(s) 775, 1134
BspT104I TTCGAA 1 cut(s) 305
BspTI CTTAAG 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 41
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 385
BssMI GATC 5 cut(s) 353, 679, 767, 1126, 1130
BssNI GRCGYC 1 cut(s) 1114
BssT1I CCWWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 527, 782
BstACI GRCGYC 1 cut(s) 1114
BstAFI CTTAAG 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 32, 214, 643, 957
BstDEI CTNAG 5 cut(s) 93, 279, 495, 696, 936
BstF5I GGATG 3 cut(s) 187, 682, 713
BstKTI GATC 5 cut(s) 356, 682, 770, 1129, 1133
BstMAI GTCTC 2 cut(s) 230, 1000
BstMBI GATC 5 cut(s) 353, 679, 767, 1126, 1130
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 1109
BstV1I GCAGC 1 cut(s) 601
BstV2I GAAGAC 1 cut(s) 462
Bsu15I ATCGAT 1 cut(s) 1129
BsuTUI ATCGAT 1 cut(s) 1129
BtsCI GGATG 3 cut(s) 187, 682, 713
BtsIMutI CAGTG 3 cut(s) 68, 319, 532
Cac8I GCNNGC 4 cut(s) 32, 214, 643, 957
CciI TCATGA 1 cut(s) 859
Cfr13I GGNCC 1 cut(s) 76
ClaI ATCGAT 1 cut(s) 1129
CseI GACGC 1 cut(s) 1103
Csp6I GTAC 3 cut(s) 243, 730, 1149
CviAII CATG 4 cut(s) 674, 860, 1042, 1046
CviQI GTAC 3 cut(s) 243, 730, 1149
DdeI CTNAG 5 cut(s) 93, 279, 495, 696, 936
DpnI GATC 5 cut(s) 355, 681, 769, 1128, 1132
DpnII GATC 5 cut(s) 353, 679, 767, 1126, 1130
DraI TTTAAA 1 cut(s) 337
DriI GACNNNNNGTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 501
Eco32I GATATC 2 cut(s) 402, 895
Eco47I GGWCC 1 cut(s) 76
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 4 cut(s) 92, 120, 1026, 1119
Eco88I CYCGRG 1 cut(s) 1031
EcoICRI GAGCTC 1 cut(s) 499
EcoO109I RGGNCCY 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 307
EcoRV GATATC 2 cut(s) 402, 895
EcoT14I CCWWGG 1 cut(s) 385
EcoT22I ATGCAT 1 cut(s) 1047
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 4 cut(s) 677, 863, 1045, 1049
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 2 cut(s) 62, 847
FatI CATG 4 cut(s) 673, 859, 1041, 1045
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 590, 608, 693
FokI GGATG 3 cut(s) 194, 669, 720
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 3 cut(s) 590, 608, 693
FspBI CTAG 3 cut(s) 195, 240, 593
GluI GCNGC 3 cut(s) 590, 608, 693
HapII CCGG 1 cut(s) 1110
HgaI GACGC 1 cut(s) 1103
Hin1I GRCGYC 1 cut(s) 1114
Hin1II CATG 4 cut(s) 677, 863, 1045, 1049
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 7 cut(s) 230, 281, 417, 436, 879, 982, 1086
HpaII CCGG 1 cut(s) 1110
HphI GGTGA 2 cut(s) 109, 490
Hpy166II GTNNAC 2 cut(s) 481, 565
Hpy188I TCNGA 9 cut(s) 235, 250, 496, 559, 655, 786, 939, 1006, 1138
Hpy188III TCNNGA 4 cut(s) 408, 421, 860, 1031
Hpy8I GTNNAC 2 cut(s) 481, 565
HpyAV CCTTC 6 cut(s) 67, 385, 713, 781, 866, 1001
HpyCH4III ACNGT 2 cut(s) 527, 782
HpyCH4V TGCA 5 cut(s) 30, 428, 778, 959, 1045
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 5 cut(s) 93, 279, 495, 696, 936
Hsp92I GRCGYC 1 cut(s) 1114
Hsp92II CATG 4 cut(s) 677, 863, 1045, 1049
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 5 cut(s) 353, 679, 767, 1126, 1130
LmnI GCTCC 5 cut(s) 41, 504, 972, 1033, 1099
LpnPI CCDG 9 cut(s) 30, 307, 406, 453, 502, 627, 884, 1123, 1131
Lsp1109I GCAGC 1 cut(s) 601
MaeI CTAG 3 cut(s) 195, 240, 593
MaeIII GTNAC 1 cut(s) 66
MalI GATC 5 cut(s) 355, 681, 769, 1128, 1132
MboI GATC 5 cut(s) 353, 679, 767, 1126, 1130
MboII GAAGA 6 cut(s) 409, 423, 426, 462, 741, 1125
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 6 cut(s) 307, 517, 537, 702, 743, 822
MlyI GAGTC 3 cut(s) 290, 976, 1095
Mph1103I ATGCAT 1 cut(s) 1047
MseI TTAA 5 cut(s) 336, 360, 446, 522, 701
MslI CAYNNNNRTG 3 cut(s) 289, 687, 990
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 1 cut(s) 1110
MspR9I CCNGG 1 cut(s) 1111
MwoI GCNNNNNNNGC 1 cut(s) 222
NciI CCSGG 1 cut(s) 1111
NdeII GATC 5 cut(s) 353, 679, 767, 1126, 1130
NlaIII CATG 4 cut(s) 677, 863, 1045, 1049
NlaIV GGNNCC 2 cut(s) 78, 968
NmuCI GTSAC 1 cut(s) 66
NsiI ATGCAT 1 cut(s) 1047
NspV TTCGAA 1 cut(s) 305
PagI TCATGA 1 cut(s) 859
PfeI GAWTC 4 cut(s) 230, 417, 436, 879
PflFI GACNNNGTC 1 cut(s) 280
PflMI CCANNNNNTGG 1 cut(s) 978
PkrI GCNGC 3 cut(s) 591, 609, 694
PleI GAGTC 3 cut(s) 289, 976, 1094
PpsI GAGTC 3 cut(s) 289, 976, 1094
PpuMI RGGWCCY 1 cut(s) 76
PsiI TTATAA 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 78, 968
PspPI GGNCC 1 cut(s) 76
PspPPI RGGWCCY 1 cut(s) 76
PsyI GACNNNGTC 1 cut(s) 280
RsaI GTAC 3 cut(s) 244, 731, 1150
RsaNI GTAC 3 cut(s) 243, 730, 1149
RseI CAYNNNNRTG 3 cut(s) 289, 687, 990
SacI GAGCTC 1 cut(s) 501
SaqAI TTAA 5 cut(s) 336, 360, 446, 522, 701
SatI GCNGC 3 cut(s) 590, 608, 693
Sau3AI GATC 5 cut(s) 353, 679, 767, 1126, 1130
Sau96I GGNCC 1 cut(s) 76
ScaI AGTACT 1 cut(s) 244
SchI GAGTC 3 cut(s) 290, 976, 1095
ScrFI CCNGG 1 cut(s) 1111
SduI GDGCHC 1 cut(s) 501
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 1 cut(s) 76
SmiMI CAYNNNNRTG 3 cut(s) 289, 687, 990
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SpeI ACTAGT 1 cut(s) 239
Sse9I AATT 6 cut(s) 307, 517, 537, 702, 743, 822
SsiI CCGC 3 cut(s) 364, 607, 693
SspI AATATT 1 cut(s) 910
SspMI CTAG 3 cut(s) 195, 240, 593
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 1109
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 2 cut(s) 527, 782
TaqI TCGA 2 cut(s) 305, 1129
TasI AATT 6 cut(s) 307, 517, 537, 702, 743, 822
TatI WGTACW 2 cut(s) 242, 1148
TauI GCSGC 2 cut(s) 610, 695
TfiI GAWTC 4 cut(s) 230, 417, 436, 879
Tru1I TTAA 5 cut(s) 336, 360, 446, 522, 701
Tru9I TTAA 5 cut(s) 336, 360, 446, 522, 701
TscAI CASTG 3 cut(s) 75, 326, 532
TseFI GTSAC 1 cut(s) 66
TseI GCWGC 1 cut(s) 589
Tsp45I GTSAC 1 cut(s) 66
TspDTI ATGAA 4 cut(s) 848, 875, 1030, 1074
TspRI CASTG 3 cut(s) 75, 326, 532
Tth111I GACNNNGTC 1 cut(s) 280
Van91I CCANNNNNTGG 1 cut(s) 978
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 1 cut(s) 307
XspI CTAG 3 cut(s) 195, 240, 593
ZrmI AGTACT 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.