Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003952

Physical Location & Seq

Genomic Coordinates Reverse (-)

30513877 .. 30517971

Gene Structure 4095 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1230200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGACCAATGCTAATCATTTTCTTTGTGATGTAACTGATGGCGAAATATTTCTCGTAGTTGAATTGAAGAAGCAAAGCTCATGCTCGATTCGGCTTACCAACCTGACTGACAACCATGTTGCTTTTAAGGTTAAGACTACATCCCCTAAGAAATACTGTGTGCGCCCAAATGTTGGTGTTATCTTGCCGAATTCAACTTCTGAGTTTTCAGTTACGATGCAAGCCCCACGAACCAGTATGACAGATATGGATTGCAAAGATAAGTTCTTAATCCAGAGCACGGTTGTTTCTTCTGCAACAACTGACGAGGATGTTACATCTAGCATGTTTTCTAAAGATGGTGGCAAGTACATTGAAGAGAAAAAGCTAAGAGTTACCCTCGTTAGTCCACCCAACTCACCACTGCTGTCACCAATGAAGGTAGACTTGAAGCAGGGGCTCGGCCATGAACATTCATTTCTAAAAGATCAATTTGGTGGAGTTGAAATCCTCCCTCCGCTGAACAAGGTTACTAACGATGTGAAGTTCACCGCTACTAGTGAGAAGCCAAAGCCATCAACGAAGACTGAGTTGAAACCATCAAAGGATGTAGAGTTGAAACCAGAAGAAGACGTTGAGCTGAAACCAGAAAAAGATTCTGAGTTGAAACCAACAAAAGATGTGGAGCTGAAGCCAGCAAAATATGAGGCGTTGGAACCAGAAAAAGATATGGAGGTGAAACCAGAAAAAGAGGCAGATTTGAAACCAAAAAAAGAGGCGGAGTTGAAACCAGTTATAGATGTGGGGCTAAAATCAGCAAAGGATGTGGAATTGAAACCGCTGAAGGCTATGGAGTTTAAACTGGCAAAAGATGTGGAGTTGAATGCAACAAAGATTGTGGAAGATTTGGAGTTGGTTAAGGATATCCAAGAGATGAAATCAAAGCTAAATGCACTTGAATTAAAGCTGAGCCAGGCTGAAGTTACTATTTTAAAGCTAACAGAGGAGAGGAGTTCAAGCATTCAAGAAACGAGAATTATGCAGCATCAATTAGCACGACTGAGTACAAGAGGTGTGGATGTGAAAAGAGTCCATGTTGGATTTCCTCCCCTATTTGTTTGTATGGTGGCGATCATCAGTGTTGCTCTCGGATACTGTCTACGCCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0008150 GO:0012505 GO:0016020 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0071944 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Motile_Sperm

Protein Analysis

383

Amino Acids

42.69

Weight (kDa)

6.7

Isoelectric Point (pI)

32.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Motile_Sperm	PF00635	19 - 112	5e-29	MSP (Major sperm protein) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003952)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04180 FvH4_6g04180 FvH4_6g04180 FvH4_6g04180 FvH4_6g04180 FvH4_6g04180 FvH4_6g04180
malus_domestica	MD00G1052100.v1.1 MD12G1230200.v1.1
prunus_persica	Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335400_v2.0.a1 Prupe.6G335500_v2.0.a1
pyrus_communis	pycom04g19010 pycom12g21400
rosa_chinensis	RchiOBHm_Chr0c30g0501241 RchiOBHm_Chr0c30g0501251 RchiOBHm_Chr3g0452761 RchiOBHm_Chr3g0452781
rosa_laevigata	RLG00000025570
rosa_multiflora	Rmu_ssc0000156.1_g000002
rosa_roxburghii	Rroxscaffold_6G00429760
rosa_rugosa	Rorug02G0643000
rosa_samantha	Rh3AG045200 Rh3BG047700 Rh3CG046200 Rh3DG046500
rosa_wichuraiana	Rw3G003560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	173
AccI	GTMKAC	2 cut(s)	423, 1138
AciI	CCGC	4 cut(s)	497, 531, 758, 818
AcoI	YGGCCR	1 cut(s)	442
AcsI	RAATTY	1 cut(s)	190
AcuI	CTGAAG	3 cut(s)	689, 842, 978
AfaI	GTAC	2 cut(s)	350, 1045
AfiI	CCNNNNNNNGG	2 cut(s)	173, 280
AhlI	ACTAGT	1 cut(s)	536
AjnI	CCWGG	1 cut(s)	951
AluBI	AGCT	7 cut(s)	78, 367, 619, 667, 925, 946, 976
AluI	AGCT	7 cut(s)	78, 367, 619, 667, 925, 946, 976
Alw21I	GWGCWC	1 cut(s)	281
AoxI	GGCC	1 cut(s)	442
ApeKI	GCWGC	1 cut(s)	1021
ApoI	RAATTY	1 cut(s)	190
Asp700I	GAANNNNTTC	1 cut(s)	48
AspLEI	GCGC	1 cut(s)	165
AsuHPI	GGTGA	4 cut(s)	390, 402, 520, 727
BanII	GRGCYC	1 cut(s)	441
BbsI	GAAGAC	2 cut(s)	569, 615
Bbv12I	GWGCWC	1 cut(s)	281
BbvI	GCAGC	1 cut(s)	1033
BccI	CCATC	4 cut(s)	32, 332, 562, 586
BcgI	CGANNNNNNTGC	2 cut(s)	1000, 1034
BciT130I	CCWGG	1 cut(s)	953
BciVI	GTATCC	1 cut(s)	1124
BcuI	ACTAGT	1 cut(s)	536
BfaI	CTAG	2 cut(s)	321, 537
BfuI	GTATCC	1 cut(s)	1124
BisI	GCNGC	1 cut(s)	1022
BlpI	GCTNAGC	1 cut(s)	947
BlsI	GCNGC	1 cut(s)	1023
Bme1390I	CCNGG	1 cut(s)	953
BmiI	GGNNCC	1 cut(s)	696
BmrFI	CCNGG	1 cut(s)	953
BmsI	GCATC	2 cut(s)	207, 1033
BpiI	GAAGAC	2 cut(s)	569, 615
BplI	GAGNNNNNCTC	2 cut(s)	363, 395
Bpu1102I	GCTNAGC	1 cut(s)	947
Bsc4I	CCNNNNNNNGG	2 cut(s)	173, 280
Bse1I	ACTGG	3 cut(s)	234, 770, 846
BseBI	CCWGG	1 cut(s)	953
BseGI	GGATG	5 cut(s)	140, 316, 592, 808, 1063
BseLI	CCNNNNNNNGG	2 cut(s)	173, 280
BseMII	CTCAG	5 cut(s)	192, 558, 630, 938, 1031
BseNI	ACTGG	3 cut(s)	234, 770, 846
BseRI	GAGGAG	2 cut(s)	998, 1003
BseXI	GCAGC	1 cut(s)	1033
BshFI	GGCC	1 cut(s)	444
BsiHKAI	GWGCWC	1 cut(s)	281
BslI	CCNNNNNNNGG	2 cut(s)	173, 280
BsmI	GAATGC	2 cut(s)	868, 999
BsnI	GGCC	1 cut(s)	444
Bsp1286I	GDGCHC	2 cut(s)	281, 441
Bsp143I	GATC	2 cut(s)	466, 1110
Bsp1720I	GCTNAGC	1 cut(s)	947
BspACI	CCGC	4 cut(s)	497, 531, 758, 818
BspANI	GGCC	1 cut(s)	444
BspCNI	CTCAG	5 cut(s)	193, 559, 631, 939, 1032
BspLI	GGNNCC	1 cut(s)	696
BsrI	ACTGG	3 cut(s)	234, 770, 846
BssMI	GATC	2 cut(s)	466, 1110
Bst2UI	CCWGG	1 cut(s)	953
Bst4CI	ACNGT	3 cut(s)	158, 283, 1136
Bst6I	CTCTTC	1 cut(s)	351
BstC8I	GCNNGC	2 cut(s)	222, 675
BstDEI	CTNAG	7 cut(s)	147, 201, 368, 567, 639, 947, 1040
BstF5I	GGATG	5 cut(s)	140, 316, 592, 808, 1063
BstHHI	GCGC	1 cut(s)	165
BstKTI	GATC	2 cut(s)	469, 1113
BstMBI	GATC	2 cut(s)	466, 1110
BstNI	CCWGG	1 cut(s)	953
BstNSI	RCATGY	1 cut(s)	328
BstSCI	CCNGG	1 cut(s)	951
BstV1I	GCAGC	1 cut(s)	1033
BstV2I	GAAGAC	2 cut(s)	569, 615
BsuI	GTATCC	1 cut(s)	1124
BsuRI	GGCC	1 cut(s)	444
BtsCI	GGATG	5 cut(s)	140, 316, 592, 808, 1063
BtsI	GCAGTG	1 cut(s)	401
BtsIMutI	CAGTG	2 cut(s)	401, 1123
Cac8I	GCNNGC	2 cut(s)	222, 675
CfoI	GCGC	1 cut(s)	165
Csp6I	GTAC	2 cut(s)	349, 1044
CviAII	CATG	5 cut(s)	81, 116, 325, 446, 1073
CviQI	GTAC	2 cut(s)	349, 1044
DdeI	CTNAG	7 cut(s)	147, 201, 368, 567, 639, 947, 1040
DpnI	GATC	2 cut(s)	468, 1112
DpnII	GATC	2 cut(s)	466, 1110
DraI	TTTAAA	2 cut(s)	838, 972
EaeI	YGGCCR	1 cut(s)	442
Eam1104I	CTCTTC	1 cut(s)	351
EarI	CTCTTC	1 cut(s)	351
EciI	GGCGGA	1 cut(s)	773
Eco24I	GRGCYC	1 cut(s)	441
Eco32I	GATATC	1 cut(s)	904
Eco57I	CTGAAG	3 cut(s)	689, 842, 978
EcoRI	GAATTC	1 cut(s)	190
EcoRII	CCWGG	1 cut(s)	951
EcoRV	GATATC	1 cut(s)	904
EcoT38I	GRGCYC	1 cut(s)	441
FaeI	CATG	5 cut(s)	84, 119, 328, 449, 1076
FalI	AAGNNNNNCTT	2 cut(s)	410, 442
FatI	CATG	5 cut(s)	80, 115, 324, 445, 1072
FblI	GTMKAC	2 cut(s)	423, 1138
Fnu4HI	GCNGC	1 cut(s)	1022
FokI	GGATG	5 cut(s)	127, 323, 599, 815, 1070
FriOI	GRGCYC	1 cut(s)	441
Fsp4HI	GCNGC	1 cut(s)	1022
FspBI	CTAG	2 cut(s)	321, 537
GlaI	GCGC	1 cut(s)	164
GluI	GCNGC	1 cut(s)	1022
HaeIII	GGCC	1 cut(s)	444
HhaI	GCGC	1 cut(s)	165
Hin1II	CATG	5 cut(s)	84, 119, 328, 449, 1076
Hin6I	GCGC	1 cut(s)	163
HinP1I	GCGC	1 cut(s)	163
HinfI	GANTC	3 cut(s)	88, 635, 1068
HphI	GGTGA	4 cut(s)	390, 402, 520, 727
Hpy166II	GTNNAC	4 cut(s)	389, 424, 528, 1139
Hpy188I	TCNGA	3 cut(s)	202, 640, 1130
Hpy188III	TCNNGA	2 cut(s)	274, 1004
Hpy8I	GTNNAC	4 cut(s)	389, 424, 528, 1139
HpyAV	CCTTC	2 cut(s)	412, 817
HpyCH4III	ACNGT	3 cut(s)	158, 283, 1136
HpyCH4IV	ACGT	1 cut(s)	612
HpyCH4V	TGCA	6 cut(s)	220, 255, 296, 866, 932, 1021
HpyF3I	CTNAG	7 cut(s)	147, 201, 368, 567, 639, 947, 1040
HpySE526I	ACGT	1 cut(s)	612
Hsp92II	CATG	5 cut(s)	84, 119, 328, 449, 1076
HspAI	GCGC	1 cut(s)	163
Kzo9I	GATC	2 cut(s)	466, 1110
LmnI	GCTCC	1 cut(s)	664
Lsp1109I	GCAGC	1 cut(s)	1033
LweI	GCATC	2 cut(s)	207, 1033
MaeI	CTAG	2 cut(s)	321, 537
MaeII	ACGT	1 cut(s)	612
MaeIII	GTNAC	7 cut(s)	31, 211, 313, 373, 408, 508, 961
MalI	GATC	2 cut(s)	468, 1112
MboI	GATC	2 cut(s)	466, 1110
MboII	GAAGA	7 cut(s)	79, 282, 368, 574, 617, 620, 893
MhlI	GDGCHC	2 cut(s)	281, 441
MluCI	AATT	7 cut(s)	62, 190, 470, 809, 938, 1014, 1028
MlyI	GAGTC	1 cut(s)	1077
MmeI	TCCRAC	2 cut(s)	672, 1057
MroXI	GAANNNNTTC	1 cut(s)	48
MseI	TTAA	7 cut(s)	126, 132, 269, 837, 897, 941, 971
MspA1I	CMGCKG	2 cut(s)	499, 820
MspR9I	CCNGG	1 cut(s)	953
MssI	GTTTAAAC	1 cut(s)	838
Mva1269I	GAATGC	2 cut(s)	868, 999
MvaI	CCWGG	1 cut(s)	953
NdeII	GATC	2 cut(s)	466, 1110
NlaIII	CATG	5 cut(s)	84, 119, 328, 449, 1076
NlaIV	GGNNCC	1 cut(s)	696
NmeAIII	GCCGAG	1 cut(s)	420
NmuCI	GTSAC	1 cut(s)	408
NspI	RCATGY	1 cut(s)	328
PctI	GAATGC	2 cut(s)	868, 999
PdmI	GAANNNNTTC	1 cut(s)	48
PfeI	GAWTC	2 cut(s)	88, 635
PflMI	CCANNNNNTGG	1 cut(s)	173
PkrI	GCNGC	1 cut(s)	1023
PleI	GAGTC	1 cut(s)	1076
PmeI	GTTTAAAC	1 cut(s)	838
PpsI	GAGTC	1 cut(s)	1076
Psp6I	CCWGG	1 cut(s)	951
PspGI	CCWGG	1 cut(s)	951
PspN4I	GGNNCC	1 cut(s)	696
RsaI	GTAC	2 cut(s)	350, 1045
RsaNI	GTAC	2 cut(s)	349, 1044
SaqAI	TTAA	7 cut(s)	126, 132, 269, 837, 897, 941, 971
SatI	GCNGC	1 cut(s)	1022
Sau3AI	GATC	2 cut(s)	466, 1110
SchI	GAGTC	1 cut(s)	1077
ScrFI	CCNGG	1 cut(s)	953
SduI	GDGCHC	2 cut(s)	281, 441
SfaNI	GCATC	2 cut(s)	207, 1033
SpeI	ACTAGT	1 cut(s)	536
Sse9I	AATT	7 cut(s)	62, 190, 470, 809, 938, 1014, 1028
SsiI	CCGC	4 cut(s)	497, 531, 758, 818
SspI	AATATT	1 cut(s)	48
SspMI	CTAG	2 cut(s)	321, 537
StyD4I	CCNGG	1 cut(s)	951
TaaI	ACNGT	3 cut(s)	158, 283, 1136
TaiI	ACGT	1 cut(s)	615
TaqI	TCGA	1 cut(s)	86
TasI	AATT	7 cut(s)	62, 190, 470, 809, 938, 1014, 1028
TatI	WGTACW	2 cut(s)	348, 1043
TfiI	GAWTC	2 cut(s)	88, 635
Tru1I	TTAA	7 cut(s)	126, 132, 269, 837, 897, 941, 971
Tru9I	TTAA	7 cut(s)	126, 132, 269, 837, 897, 941, 971
TscAI	CASTG	2 cut(s)	408, 1123
TseFI	GTSAC	1 cut(s)	408
TseI	GCWGC	1 cut(s)	1021
Tsp45I	GTSAC	1 cut(s)	408
TspDTI	ATGAA	4 cut(s)	431, 444, 462, 929
TspRI	CASTG	2 cut(s)	408, 1123
Van91I	CCANNNNNTGG	1 cut(s)	173
XapI	RAATTY	1 cut(s)	190
XceI	RCATGY	1 cut(s)	328
XmiI	GTMKAC	2 cut(s)	423, 1138
XmnI	GAANNNNTTC	1 cut(s)	48
XspI	CTAG	2 cut(s)	321, 537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.