Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002085

Physical Location & Seq

Genomic Coordinates Forward (+)

31689446 .. 31694912

Gene Structure 5467 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1246700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1023 bp

ATGCGGGCTGCAATGGCGTCGGCTGAAGTTGATGATGATGTACTCGGGGCCGACCCAACGGCATTCCGCTTAGAGTCGGAAATGGCAAAGGTCATGGGGAAGGAAGCGGCACTTTTTGTTCCGTCAGGCACAATGGGAAACCTCATCAGTGTACTTGTGCACTGTGACATCCGAGGAAGCGAAGTCATTCTCGGACACAATTCTCACATTCACATTTACGAGAATGGAGGCATCGCCACAATCGGAGGTGTGCACCCGAGAACGGTTAGAAACAACAAAGACGGAACCATGGATCTTGATGCGATCGAAGCCTCCATCAGAGATCCGAGAGGGGAGCTTGTGTATCCGACGACAAGGCTTATTTGCTTGGAAAATTCGCAGGCAAACTGCGGTGGTAGATGCCTTACTGTAGAGTACACAGACAAAGTGGGAGAAATAGCCAAGAAACATGGTTTGAAGCTCCACATTGACGGGGCTCGAATTTTCAATGCAGCAATTGCACTCGGTGTTCCTGTTGATAGGCTTGTGCAGGCTGCTGATTCAGTTTCGGTTTGTCTGTCAAAAGGTATTGGTGCTCCTGTTGGATCTGTCATTGTTGGTTCGAAAAGCTTTATTGCTAAGGCTAGAAGGCTCAAGAAAACCTTAGGCGGCGGAATGAGACAGGTTGGTATCCTTTGTGCTGCTGCTTTGGTCGGTTTACATGACAATGTTGCGAAGCTTGAGACCGATCACAAGCGAGCTAAGATTTTAGCAGAGGGACTAAATCAAATCAAAGGACTGAGTGTAGATGTCGCTTCGGTTGAGACCAACATTTTATACATACACGTCGAAGAGGGCTCAAATGTTACTACCGAAAAACTAGTCAAGAACTTGGAAGAACACGGTGTACTCATGATGCAAGAGAGCTCGTCAAGCATTAGGATAGTTATCCACCACCAAATCTCTGCAAGTGATGTGCAATACACTCTATCCTGCTTTCAGAAAGCTCTGACTGGTGGAGTCCTTGAAGAAAATGGAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0003824 GO:0004793 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006544 GO:0006545 GO:0006566 GO:0006567 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0008732 GO:0009056 GO:0009058 GO:0009063 GO:0009066 GO:0009068 GO:0009069 GO:0009070 GO:0009987 GO:0016053 GO:0016054 GO:0016829 GO:0016830 GO:0016832 GO:0017144 GO:0019752 GO:0042133 GO:0042136 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0065007 GO:0065008 GO:0071704 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko01100 ko01110 ko01120 ko01130 ko01230 map00260 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Beta_elim_lyase

Protein Analysis

341

Amino Acids

36.16

Weight (kDa)

6.36

Isoelectric Point (pI)

28.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta_elim_lyase	PF01212	1 - 275	4e-98	Beta-eliminating lyase
Aminotran_1_2	PF00155	24 - 313	7.7e-11	Aminotransferase class I and II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002085)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08630 AT1G08630 AT1G08630 AT1G08630 AT1G08630 AT1G08630 AT1G08630 AT3G04520 AT3G04520 AT3G04520
fragaria_vesca	FvH4_6g01941 FvH4_6g01941 FvH4_6g01941 FvH4_6g01941 FvH4_6g01941 FvH4_6g01941 FvH4_6g01941 FvH4_6g01941
malus_domestica	MD12G1246700.v1.1
prunus_persica	Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1 Prupe.6G349500_v2.0.a1
pyrus_communis	pycom12g22610
rosa_chinensis	RchiOBHm_Chr3g0449781
rosa_laevigata	RLG00000025800
rosa_multiflora	Rmu_sc0000610.1_g000012
rosa_roxburghii	Rroxscaffold_6G00427610
rosa_rugosa	Rorug02G0622900 Rorug02G0623000 Rorug02G0623100 Rorug02G0623200 Rorug02G0623300
rosa_samantha	Rh3AG021500 Rh3BG022200 Rh3CG020800 Rh3DG021900
rosa_wichuraiana	Rw3G001760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	6 cut(s)	4, 67, 107, 390, 648, 651
AclWI	GGATC	3 cut(s)	300, 317, 592
AcsI	RAATTY	2 cut(s)	373, 480
AcuI	CTGAAG	1 cut(s)	45
AcyI	GRCGYC	1 cut(s)	17
AdeI	CACNNNGTG	1 cut(s)	506
AfaI	GTAC	4 cut(s)	42, 153, 416, 888
AfiI	CCNNNNNNNGG	1 cut(s)	262
AflIII	ACRYGT	1 cut(s)	823
AgsI	TTSAA	3 cut(s)	457, 487, 1007
AhlI	ACTAGT	1 cut(s)	859
AjiI	CACGTC	1 cut(s)	826
AluBI	AGCT	7 cut(s)	337, 460, 609, 718, 740, 906, 986
AluI	AGCT	7 cut(s)	337, 460, 609, 718, 740, 906, 986
Alw21I	GWGCWC	4 cut(s)	162, 255, 577, 908
Alw26I	GTCTC	3 cut(s)	652, 716, 797
Alw44I	GTGCAC	2 cut(s)	158, 251
AlwI	GGATC	3 cut(s)	300, 317, 592
Ama87I	CYCGRG	2 cut(s)	44, 256
AoxI	GGCC	1 cut(s)	48
ApaLI	GTGCAC	2 cut(s)	158, 251
ApeKI	GCWGC	5 cut(s)	8, 491, 533, 680, 683
ApoI	RAATTY	2 cut(s)	373, 480
ArsI	GACNNNNNNTTYG	2 cut(s)	846, 878
Asp700I	GAANNNNTTC	1 cut(s)	186
AspS9I	GGNCC	1 cut(s)	48
AsuII	TTCGAA	1 cut(s)	602
AvaI	CYCGRG	2 cut(s)	44, 256
AxyI	CCTNAGG	1 cut(s)	643
BaeGI	GKGCMC	2 cut(s)	162, 255
BanII	GRGCYC	3 cut(s)	478, 839, 908
Bbv12I	GWGCWC	4 cut(s)	162, 255, 577, 908
BbvI	GCAGC	4 cut(s)	503, 520, 667, 670
BccI	CCATC	1 cut(s)	323
BceAI	ACGGC	1 cut(s)	75
BcgI	CGANNNNNNTGC	1 cut(s)	34
BciVI	GTATCC	2 cut(s)	354, 680
BcoDI	GTCTC	3 cut(s)	652, 716, 797
BcuI	ACTAGT	1 cut(s)	859
BfaI	CTAG	2 cut(s)	624, 860
BfmI	CTRYAG	1 cut(s)	408
BfuI	GTATCC	2 cut(s)	354, 680
BisI	GCNGC	7 cut(s)	9, 108, 492, 534, 649, 681, 684
BlsI	GCNGC	7 cut(s)	10, 109, 493, 535, 650, 682, 685
BmeT110I	CYCGRG	2 cut(s)	44, 256
BmgBI	CACGTC	1 cut(s)	826
BmgT120I	GGNCC	1 cut(s)	48
BmiI	GGNNCC	2 cut(s)	49, 286
BmsI	GCATC	4 cut(s)	240, 289, 389, 885
Bpu10I	CCTNAGC	1 cut(s)	618
Bpu14I	TTCGAA	1 cut(s)	602
BpuEI	CTTGAG	2 cut(s)	617, 740
BsaBI	GATNNNNATC	1 cut(s)	926
BsaHI	GRCGYC	1 cut(s)	17
BsaI	GGTCTC	2 cut(s)	716, 797
BsaJI	CCNNGG	2 cut(s)	172, 288
BsaXI	ACNNNNNCTCC	2 cut(s)	326, 356
Bsc4I	CCNNNNNNNGG	1 cut(s)	262
Bse1I	ACTGG	1 cut(s)	997
Bse21I	CCTNAGG	1 cut(s)	643
Bse3DI	GCAATG	1 cut(s)	18
Bse8I	GATNNNNATC	1 cut(s)	926
BseDI	CCNNGG	2 cut(s)	172, 288
BseGI	GGATG	1 cut(s)	168
BseJI	GATNNNNATC	1 cut(s)	926
BseLI	CCNNNNNNNGG	1 cut(s)	262
BseMI	GCAATG	1 cut(s)	18
BseMII	CTCAG	1 cut(s)	770
BseNI	ACTGG	1 cut(s)	997
BseSI	GKGCMC	2 cut(s)	162, 255
BseXI	GCAGC	4 cut(s)	503, 520, 667, 670
BsgI	GTGCAG	1 cut(s)	548
Bsh1285I	CGRYCG	1 cut(s)	306
BshFI	GGCC	1 cut(s)	50
BsiEI	CGRYCG	1 cut(s)	306
BsiHKAI	GWGCWC	4 cut(s)	162, 255, 577, 908
BsiHKCI	CYCGRG	2 cut(s)	44, 256
BslFI	GGGAC	1 cut(s)	771
BslI	CCNNNNNNNGG	1 cut(s)	262
BsmAI	GTCTC	3 cut(s)	652, 716, 797
BsmFI	GGGAC	1 cut(s)	771
BsmI	GAATGC	1 cut(s)	62
BsnI	GGCC	1 cut(s)	50
Bso31I	GGTCTC	2 cut(s)	716, 797
BsoBI	CYCGRG	2 cut(s)	44, 256
Bsp119I	TTCGAA	1 cut(s)	602
Bsp1286I	GDGCHC	6 cut(s)	162, 255, 478, 577, 839, 908
Bsp143I	GATC	5 cut(s)	292, 303, 322, 584, 727
Bsp19I	CCATGG	1 cut(s)	288
BspACI	CCGC	6 cut(s)	4, 67, 107, 390, 648, 651
BspANI	GGCC	1 cut(s)	50
BspCNI	CTCAG	1 cut(s)	771
BspHI	TCATGA	1 cut(s)	891
BspLI	GGNNCC	2 cut(s)	49, 286
BspPI	GGATC	3 cut(s)	300, 317, 592
BspT104I	TTCGAA	1 cut(s)	602
BspTNI	GGTCTC	2 cut(s)	716, 797
BsrDI	GCAATG	1 cut(s)	18
BsrI	ACTGG	1 cut(s)	997
BssECI	CCNNGG	2 cut(s)	172, 288
BssMI	GATC	5 cut(s)	292, 303, 322, 584, 727
BssNI	GRCGYC	1 cut(s)	17
BssT1I	CCWWGG	1 cut(s)	288
Bst4CI	ACNGT	4 cut(s)	164, 265, 409, 884
Bst6I	CTCTTC	1 cut(s)	825
BstACI	GRCGYC	1 cut(s)	17
BstAPI	GCANNNNNTGC	1 cut(s)	497
BstBI	TTCGAA	1 cut(s)	602
BstC8I	GCNNGC	4 cut(s)	6, 381, 531, 738
BstDEI	CTNAG	5 cut(s)	70, 618, 643, 741, 779
BstDSI	CCRYGG	1 cut(s)	288
BstF5I	GGATG	1 cut(s)	168
BstKTI	GATC	5 cut(s)	295, 306, 325, 587, 730
BstMAI	GTCTC	3 cut(s)	652, 716, 797
BstMBI	GATC	5 cut(s)	292, 303, 322, 584, 727
BstMCI	CGRYCG	1 cut(s)	306
BstMWI	GCNNNNNNNGC	4 cut(s)	14, 308, 497, 912
BstSFI	CTRYAG	1 cut(s)	408
BstSLI	GKGCMC	2 cut(s)	162, 255
BstV1I	GCAGC	4 cut(s)	503, 520, 667, 670
BstX2I	RGATCY	3 cut(s)	292, 322, 584
BstYI	RGATCY	3 cut(s)	292, 322, 584
Bsu36I	CCTNAGG	1 cut(s)	643
BsuI	GTATCC	2 cut(s)	354, 680
BsuRI	GGCC	1 cut(s)	50
BtgI	CCRYGG	1 cut(s)	288
BtgZI	GCGATG	1 cut(s)	217
BtrI	CACGTC	1 cut(s)	826
BtsCI	GGATG	1 cut(s)	168
BtsIMutI	CAGTG	2 cut(s)	154, 160
Cac8I	GCNNGC	4 cut(s)	6, 381, 531, 738
CciI	TCATGA	1 cut(s)	891
Cfr13I	GGNCC	1 cut(s)	48
CseI	GACGC	1 cut(s)	6
Csp6I	GTAC	4 cut(s)	41, 152, 415, 887
CviAII	CATG	5 cut(s)	94, 289, 449, 701, 892
CviQI	GTAC	4 cut(s)	41, 152, 415, 887
DdeI	CTNAG	5 cut(s)	70, 618, 643, 741, 779
DpnI	GATC	5 cut(s)	294, 305, 324, 586, 729
DpnII	GATC	5 cut(s)	292, 303, 322, 584, 727
DraIII	CACNNNGTG	1 cut(s)	506
Eam1104I	CTCTTC	1 cut(s)	825
EarI	CTCTTC	1 cut(s)	825
EciI	GGCGGA	1 cut(s)	666
Ecl136II	GAGCTC	1 cut(s)	906
Eco130I	CCWWGG	1 cut(s)	288
Eco24I	GRGCYC	3 cut(s)	478, 839, 908
Eco31I	GGTCTC	2 cut(s)	716, 797
Eco53kI	GAGCTC	1 cut(s)	906
Eco57I	CTGAAG	1 cut(s)	45
Eco81I	CCTNAGG	1 cut(s)	643
Eco88I	CYCGRG	2 cut(s)	44, 256
EcoICRI	GAGCTC	1 cut(s)	906
EcoT14I	CCWWGG	1 cut(s)	288
EcoT38I	GRGCYC	3 cut(s)	478, 839, 908
ErhI	CCWWGG	1 cut(s)	288
FaeI	CATG	5 cut(s)	97, 292, 452, 704, 895
FaiI	YATR	7 cut(s)	95, 290, 450, 702, 817, 821, 893
FalI	AAGNNNNNCTT	4 cut(s)	96, 128, 626, 658
FaqI	GGGAC	1 cut(s)	771
FatI	CATG	5 cut(s)	93, 288, 448, 700, 891
Fnu4HI	GCNGC	7 cut(s)	9, 108, 492, 534, 649, 681, 684
FokI	GGATG	1 cut(s)	155
FriOI	GRGCYC	3 cut(s)	478, 839, 908
Fsp4HI	GCNGC	7 cut(s)	9, 108, 492, 534, 649, 681, 684
FspBI	CTAG	2 cut(s)	624, 860
GluI	GCNGC	7 cut(s)	9, 108, 492, 534, 649, 681, 684
HaeIII	GGCC	1 cut(s)	50
HgaI	GACGC	1 cut(s)	6
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	5 cut(s)	97, 292, 452, 704, 895
HindIII	AAGCTT	2 cut(s)	607, 716
HinfI	GANTC	3 cut(s)	74, 539, 999
Hpy166II	GTNNAC	6 cut(s)	152, 160, 253, 417, 698, 887
Hpy188I	TCNGA	9 cut(s)	79, 173, 194, 245, 320, 327, 348, 981, 990
Hpy188III	TCNNGA	4 cut(s)	296, 634, 865, 892
Hpy8I	GTNNAC	6 cut(s)	152, 160, 253, 417, 698, 887
Hpy99I	CGWCG	3 cut(s)	22, 352, 830
HpyAV	CCTTC	2 cut(s)	94, 621
HpyCH4III	ACNGT	4 cut(s)	164, 265, 409, 884
HpyCH4IV	ACGT	1 cut(s)	825
HpyCH4V	TGCA	9 cut(s)	11, 160, 253, 491, 500, 529, 898, 947, 958
HpyF10VI	GCNNNNNNNGC	4 cut(s)	14, 308, 497, 912
HpyF3I	CTNAG	5 cut(s)	70, 618, 643, 741, 779
HpySE526I	ACGT	1 cut(s)	825
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	5 cut(s)	97, 292, 452, 704, 895
Kzo9I	GATC	5 cut(s)	292, 303, 322, 584, 727
LmnI	GCTCC	3 cut(s)	334, 465, 580
LpnPI	CCDG	8 cut(s)	111, 365, 515, 525, 591, 647, 978, 985
Lsp1109I	GCAGC	4 cut(s)	503, 520, 667, 670
LweI	GCATC	4 cut(s)	240, 289, 389, 885
MaeI	CTAG	2 cut(s)	624, 860
MaeII	ACGT	1 cut(s)	825
MaeIII	GTNAC	2 cut(s)	164, 844
MalI	GATC	5 cut(s)	294, 305, 324, 586, 729
MboI	GATC	5 cut(s)	292, 303, 322, 584, 727
MboII	GAAGA	3 cut(s)	842, 887, 1019
MfeI	CAATTG	1 cut(s)	495
MflI	RGATCY	3 cut(s)	292, 322, 584
MhlI	GDGCHC	6 cut(s)	162, 255, 478, 577, 839, 908
MluCI	AATT	4 cut(s)	199, 373, 480, 495
MlyI	GAGTC	2 cut(s)	83, 1008
MmeI	TCCRAC	3 cut(s)	57, 371, 562
MnlI	CCTC	8 cut(s)	152, 167, 221, 239, 322, 323, 748, 826
MroXI	GAANNNNTTC	1 cut(s)	186
MslI	CAYNNNNRTG	1 cut(s)	705
MunI	CAATTG	1 cut(s)	495
Mva1269I	GAATGC	1 cut(s)	62
MwoI	GCNNNNNNNGC	4 cut(s)	14, 308, 497, 912
NcoI	CCATGG	1 cut(s)	288
NdeII	GATC	5 cut(s)	292, 303, 322, 584, 727
NlaIII	CATG	5 cut(s)	97, 292, 452, 704, 895
NlaIV	GGNNCC	2 cut(s)	49, 286
NmuCI	GTSAC	1 cut(s)	164
NspV	TTCGAA	1 cut(s)	602
PagI	TCATGA	1 cut(s)	891
PctI	GAATGC	1 cut(s)	62
PdmI	GAANNNNTTC	1 cut(s)	186
PfeI	GAWTC	1 cut(s)	539
PkrI	GCNGC	7 cut(s)	10, 109, 493, 535, 650, 682, 685
Ple19I	CGATCG	1 cut(s)	306
PleI	GAGTC	2 cut(s)	82, 1007
PpsI	GAGTC	2 cut(s)	82, 1007
Psp124BI	GAGCTC	1 cut(s)	908
PspN4I	GGNNCC	2 cut(s)	49, 286
PspPI	GGNCC	1 cut(s)	48
PsrI	GAACNNNNNNTAC	2 cut(s)	870, 902
PsuI	RGATCY	3 cut(s)	292, 322, 584
PvuI	CGATCG	1 cut(s)	306
RsaI	GTAC	4 cut(s)	42, 153, 416, 888
RsaNI	GTAC	4 cut(s)	41, 152, 415, 887
RseI	CAYNNNNRTG	1 cut(s)	705
SacI	GAGCTC	1 cut(s)	908
SatI	GCNGC	7 cut(s)	9, 108, 492, 534, 649, 681, 684
Sau3AI	GATC	5 cut(s)	292, 303, 322, 584, 727
Sau96I	GGNCC	1 cut(s)	48
SchI	GAGTC	2 cut(s)	83, 1008
SduI	GDGCHC	6 cut(s)	162, 255, 478, 577, 839, 908
SfaNI	GCATC	4 cut(s)	240, 289, 389, 885
SfcI	CTRYAG	1 cut(s)	408
SfuI	TTCGAA	1 cut(s)	602
SmiMI	CAYNNNNRTG	1 cut(s)	705
SmlI	CTYRAG	2 cut(s)	632, 719
SmoI	CTYRAG	2 cut(s)	632, 719
SpeI	ACTAGT	1 cut(s)	859
Sse9I	AATT	4 cut(s)	199, 373, 480, 495
SsiI	CCGC	6 cut(s)	4, 67, 107, 390, 648, 651
SspMI	CTAG	2 cut(s)	624, 860
SstI	GAGCTC	1 cut(s)	908
StyI	CCWWGG	1 cut(s)	288
TaaI	ACNGT	4 cut(s)	164, 265, 409, 884
TaiI	ACGT	1 cut(s)	828
TaqI	TCGA	4 cut(s)	306, 478, 602, 828
TaqII	GACCGA	1 cut(s)	740
TasI	AATT	4 cut(s)	199, 373, 480, 495
TatI	WGTACW	4 cut(s)	40, 151, 414, 886
TauI	GCSGC	2 cut(s)	110, 651
TfiI	GAWTC	1 cut(s)	539
TscAI	CASTG	2 cut(s)	154, 167
TseFI	GTSAC	1 cut(s)	164
TseI	GCWGC	5 cut(s)	8, 491, 533, 680, 683
Tsp45I	GTSAC	1 cut(s)	164
TspGWI	ACGGA	2 cut(s)	111, 297
TspRI	CASTG	2 cut(s)	154, 167
VneI	GTGCAC	2 cut(s)	158, 251
XapI	RAATTY	2 cut(s)	373, 480
XmnI	GAANNNNTTC	1 cut(s)	186
XspI	CTAG	2 cut(s)	624, 860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.