MD13G1001600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011357
Physical Location & Seq
Forward (+)
110643 .. 111989
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1001600.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGCCATCCGAGACACCAACACCGTCGTCGGAGAGCAGCCATGGCGGCACCTACCTCCAAAGCCTCCTGGACACGGCCCGCCCCTTCCTCCGGGGTGAGCTGGAGTCCGTTGACAAAAATCTGCCTTCTCTGGTGGCCGTCCTGCGCTCAGTTGGAGCTGGCGAGTGCTGGCATAAGCACGGCACCTTTCTCGAACACCTGGTTGACATCTACCGCATTCTCAAGATATGGAAGGCCCCTGACTCTGTCTGCCTCTGCGGCCTCTTTCACTCCGCTTATTCCAATTCATATGTCAACCTTGCCATCTTTGATCCTTCCACAGGCCGCGAAGTCGTTCGCGCCCATGTCGGGGAGGCTGCGGAACGCTTGATTCACTTGTTCTGCATTGTCCCCAGACAGCCTCTCATCCATGACGACCTTTTGTTCCACTACACCGACGCAGAGCTCGTGGAGCATCTCAAACAATCAGAGATGTCGCTGCAGCAGTTGCAGAACCAATCGGAAGCCGCAAGTCCGAATTGGAGTTGGAGGAACAAGGTGCAGTGTCTTGTTCCTGCAAATGGGGTTACAGTGAAGCACATAAAGACTGGGGAAGATGTGTTGGTATCGAGGAGGGTACTTGCAGTGTTTGTCCTGATGACAATGGCAGATTTCAGTGATCAACTCTTTGGTTTCCAGGACGAGCTCTTGGATAACAAAGATGGTCGCCTCGAGTTCAGGGGCAACAACTATGGAGCTCTGTGGCCAGGCGATGGCAAGCCAGGGCTTTGGATGAATTCCATATCAAGGATGGCTGCAGTGTACACTTTGATAGTGAGAGAGGAGGAGATGTTTATCCAAGAGAAGAAAACTATTAGTTCTGTTAATGCTACTGCTGGAAGTGGAGGAGGAGAGAATGCCGCGACGGACAAATGTAGAGATGAAGACATCGAGCTGGTGGTGCCACCAGTGTTTGACAAATGCACAAGGGTTCTGGATGCGAAAGAGCAATTGGCTGCAAGGGAATTGTACTGGGAAGCTGTTTGTGATGTGTCTCAAAAACAGGGAAAGAAAGGAGAGGAGGACAGAGCTGAGGAGTTGCTGTTGAGGTGTGTTGAGAAGAACCCTTTTATTGGGGAGCCGCATGTGGTGTTGGGTCAGGTTTATTTGACAGGAGCAAGGTTTGAGGAGGCGGAGAGGGAGGCAGAGAGAGGGCTAACCCTGATGCTGGAATGGGGGAGTGCTTGGGACAAGCGGATGACTTGGGAAGGTTGGATTGCTTGGGCAAGGGTGCTCCTCATGAAGGCAAAGGAGAGAAGTTGGCCACAAACTTCCTGGGGCATCCTCAACTTGGGCCTTGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

449

Amino Acids

50.47

Weight (kDa)

5.43

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6817 PF20680 47 - 132 6e-30 Domain of unknown function (DUF6817)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 47, 182, 940
AccB7I CCANNNNNTGG 1 cut(s) 752
AccII CGCG 3 cut(s) 327, 339, 902
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 3 cut(s) 135, 743, 1301
AcsI RAATTY 1 cut(s) 775
AfaI GTAC 3 cut(s) 618, 803, 1010
AjnI CCWGG 6 cut(s) 66, 198, 675, 745, 760, 1313
AjuI GAANNNNNNNTTGG 2 cut(s) 974, 1006
AluBI AGCT 8 cut(s) 100, 158, 445, 685, 737, 934, 1019, 1070
AluI AGCT 8 cut(s) 100, 158, 445, 685, 737, 934, 1019, 1070
Alw21I GWGCWC 4 cut(s) 447, 687, 739, 1275
Alw26I GTCTC 2 cut(s) 5, 1038
AlwI GGATC 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 710
AoxI GGCC 8 cut(s) 75, 135, 234, 259, 322, 743, 1301, 1333
ApeKI GCWGC 6 cut(s) 36, 356, 478, 481, 794, 995
ApoI RAATTY 1 cut(s) 775
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 2 cut(s) 147, 341
AspS9I GGNCC 3 cut(s) 76, 235, 1333
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 107
AvaI CYCGRG 1 cut(s) 710
BalI TGGCCA 2 cut(s) 745, 1303
BanI GGYRCC 3 cut(s) 47, 182, 940
BanII GRGCYC 3 cut(s) 447, 687, 739
BauI CACGAG 1 cut(s) 446
BbsI GAAGAC 1 cut(s) 930
Bbv12I GWGCWC 4 cut(s) 447, 687, 739, 1275
BbvCI CCTCAGC 1 cut(s) 1071
BbvI GCAGC 6 cut(s) 48, 343, 465, 493, 781, 982
BccI CCATC 5 cut(s) 13, 311, 695, 746, 784
BceAI ACGGC 3 cut(s) 90, 122, 196
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 6 cut(s) 68, 200, 677, 747, 762, 1315
BclI TGATCA 1 cut(s) 658
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 5, 1038
BfmI CTRYAG 2 cut(s) 479, 795
BglI GCCNNNNNGGC 2 cut(s) 45, 258
Bme1390I CCNGG 7 cut(s) 68, 92, 200, 677, 747, 762, 1315
BmeT110I CYCGRG 1 cut(s) 710
BmgT120I GGNCC 3 cut(s) 76, 235, 1333
BmiI GGNNCC 5 cut(s) 49, 184, 237, 942, 1119
BmrFI CCNGG 7 cut(s) 68, 92, 200, 677, 747, 762, 1315
BmrI ACTGGG 2 cut(s) 597, 1021
BmsI GCATC 4 cut(s) 463, 967, 1194, 1329
BmuI ACTGGG 2 cut(s) 597, 1021
BpiI GAAGAC 1 cut(s) 930
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 1071
BpuEI CTTGAG 1 cut(s) 206
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 833
BsaJI CCNNGG 4 cut(s) 40, 91, 761, 1314
BsaXI ACNNNNNCTCC 4 cut(s) 815, 845, 1067, 1097
Bse1I ACTGG 3 cut(s) 592, 947, 1016
Bse8I GATNNNNATC 1 cut(s) 833
BseBI CCWGG 6 cut(s) 68, 200, 677, 747, 762, 1315
BseDI CCNNGG 4 cut(s) 40, 91, 761, 1314
BseGI GGATG 7 cut(s) 5, 405, 777, 795, 982, 1242, 1320
BseJI GATNNNNATC 1 cut(s) 833
BseMII CTCAG 2 cut(s) 162, 1062
BseNI ACTGG 3 cut(s) 592, 947, 1016
BseRI GAGGAG 9 cut(s) 625, 836, 839, 900, 903, 1073, 1088, 1181, 1265
BseXI GCAGC 6 cut(s) 48, 343, 465, 493, 781, 982
BsgI GTGCAG 1 cut(s) 560
Bsh1236I CGCG 3 cut(s) 327, 339, 902
BshFI GGCC 8 cut(s) 77, 137, 236, 261, 324, 745, 1303, 1335
BshNI GGYRCC 3 cut(s) 47, 182, 940
BsiHKAI GWGCWC 4 cut(s) 447, 687, 739, 1275
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 1 cut(s) 91
BslFI GGGAC 2 cut(s) 374, 1241
BsmAI GTCTC 2 cut(s) 5, 1038
BsmFI GGGAC 2 cut(s) 374, 1241
BsmI GAATGC 2 cut(s) 216, 901
BsnI GGCC 8 cut(s) 77, 137, 236, 261, 324, 745, 1303, 1335
BsoBI CYCGRG 1 cut(s) 710
Bsp1286I GDGCHC 4 cut(s) 447, 687, 739, 1275
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 2 cut(s) 310, 658
Bsp19I CCATGG 1 cut(s) 40
BspANI GGCC 8 cut(s) 77, 137, 236, 261, 324, 745, 1303, 1335
BspCNI CTCAG 2 cut(s) 161, 1063
BspFNI CGCG 3 cut(s) 327, 339, 902
BspHI TCATGA 1 cut(s) 1278
BspLI GGNNCC 5 cut(s) 49, 184, 237, 942, 1119
BspMAI CTGCAG 2 cut(s) 483, 799
BspPI GGATC 1 cut(s) 305
BspT107I GGYRCC 3 cut(s) 47, 182, 940
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 3 cut(s) 592, 947, 1016
BssECI CCNNGG 4 cut(s) 40, 91, 761, 1314
BssMI GATC 2 cut(s) 310, 658
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 6 cut(s) 68, 200, 677, 747, 762, 1315
Bst4CI ACNGT 2 cut(s) 24, 571
BstAPI GCANNNNNTGC 1 cut(s) 487
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 4 cut(s) 79, 160, 170, 758
BstDEI CTNAG 2 cut(s) 148, 1071
BstDSI CCRYGG 1 cut(s) 40
BstENI CCTNNNNNAGG 2 cut(s) 318, 1280
BstF5I GGATG 7 cut(s) 5, 405, 777, 795, 982, 1242, 1320
BstFNI CGCG 3 cut(s) 327, 339, 902
BstHHI GCGC 2 cut(s) 147, 341
BstKTI GATC 2 cut(s) 313, 661
BstMAI GTCTC 2 cut(s) 5, 1038
BstMBI GATC 2 cut(s) 310, 658
BstMWI GCNNNNNNNGC 6 cut(s) 42, 45, 258, 451, 487, 940
BstNI CCWGG 6 cut(s) 68, 200, 677, 747, 762, 1315
BstNSI RCATGY 1 cut(s) 1127
BstSCI CCNGG 7 cut(s) 66, 90, 198, 675, 745, 760, 1313
BstSFI CTRYAG 2 cut(s) 479, 795
BstUI CGCG 3 cut(s) 327, 339, 902
BstV1I GCAGC 6 cut(s) 48, 343, 465, 493, 781, 982
BstV2I GAAGAC 1 cut(s) 930
BstXI CCANNNNNNTGG 1 cut(s) 768
BsuRI GGCC 8 cut(s) 77, 137, 236, 261, 324, 745, 1303, 1335
BtgI CCRYGG 1 cut(s) 40
BtgZI GCGATG 1 cut(s) 765
BtsCI GGATG 7 cut(s) 5, 405, 777, 795, 982, 1242, 1320
BtsI GCAGTG 3 cut(s) 548, 630, 804
BtsIMutI CAGTG 6 cut(s) 548, 576, 630, 661, 804, 954
Cac8I GCNNGC 4 cut(s) 79, 160, 170, 758
CciI TCATGA 1 cut(s) 1278
CfoI GCGC 2 cut(s) 147, 341
Cfr13I GGNCC 3 cut(s) 76, 235, 1333
CseI GACGC 1 cut(s) 446
CsiI ACCWGGT 1 cut(s) 198
Csp6I GTAC 3 cut(s) 617, 802, 1009
CviAII CATG 5 cut(s) 41, 344, 410, 1124, 1279
CviQI GTAC 3 cut(s) 617, 802, 1009
DdeI CTNAG 2 cut(s) 148, 1071
DpnI GATC 2 cut(s) 312, 660
DpnII GATC 2 cut(s) 310, 658
EaeI YGGCCR 3 cut(s) 135, 743, 1301
EciI GGCGGA 1 cut(s) 1187
Ecl136II GAGCTC 3 cut(s) 445, 685, 737
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 3 cut(s) 447, 687, 739
Eco53kI GAGCTC 3 cut(s) 445, 685, 737
Eco88I CYCGRG 1 cut(s) 710
EcoICRI GAGCTC 3 cut(s) 445, 685, 737
EcoNI CCTNNNNNAGG 2 cut(s) 318, 1280
EcoO109I RGGNCCY 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 775
EcoRII CCWGG 6 cut(s) 66, 198, 675, 745, 760, 1313
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 3 cut(s) 447, 687, 739
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 5 cut(s) 44, 347, 413, 1127, 1282
FaqI GGGAC 2 cut(s) 374, 1241
FatI CATG 5 cut(s) 40, 343, 409, 1123, 1278
FauI CCCGC 1 cut(s) 86
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 658
FokI GGATG 6 cut(s) 392, 784, 802, 989, 1249, 1307
FriOI GRGCYC 3 cut(s) 447, 687, 739
GlaI GCGC 2 cut(s) 146, 340
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 8 cut(s) 77, 137, 236, 261, 324, 745, 1303, 1335
HapII CCGG 1 cut(s) 91
HgaI GACGC 1 cut(s) 446
HhaI GCGC 2 cut(s) 147, 341
Hin1II CATG 5 cut(s) 44, 347, 413, 1127, 1282
Hin6I GCGC 2 cut(s) 145, 339
HinP1I GCGC 2 cut(s) 145, 339
HincII GTYRAC 3 cut(s) 112, 205, 295
HindII GTYRAC 3 cut(s) 112, 205, 295
HinfI GANTC 3 cut(s) 104, 242, 370
HpaII CCGG 1 cut(s) 91
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 5 cut(s) 112, 205, 295, 802, 804
Hpy188I TCNGA 6 cut(s) 10, 31, 469, 502, 516, 1343
Hpy188III TCNNGA 5 cut(s) 191, 223, 634, 974, 1279
Hpy8I GTNNAC 5 cut(s) 112, 205, 295, 802, 804
Hpy99I CGWCG 4 cut(s) 28, 31, 440, 907
HpyAV CCTTC 6 cut(s) 94, 135, 226, 324, 1241, 1276
HpyCH4III ACNGT 2 cut(s) 24, 571
HpyCH4V TGCA 9 cut(s) 384, 481, 490, 541, 557, 623, 797, 963, 998
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 45, 258, 451, 487, 940
HpyF3I CTNAG 2 cut(s) 148, 1071
Hsp92II CATG 5 cut(s) 44, 347, 413, 1127, 1282
HspAI GCGC 2 cut(s) 145, 339
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 2 cut(s) 310, 658
LmnI GCTCC 6 cut(s) 155, 451, 734, 1117, 1154, 1278
Lsp1109I GCAGC 6 cut(s) 48, 343, 465, 493, 781, 982
LweI GCATC 4 cut(s) 463, 967, 1194, 1329
MabI ACCWGGT 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 565
MalI GATC 2 cut(s) 312, 660
MboI GATC 2 cut(s) 310, 658
MboII GAAGA 4 cut(s) 605, 856, 935, 1111
MfeI CAATTG 1 cut(s) 989
MhlI GDGCHC 4 cut(s) 447, 687, 739, 1275
MlsI TGGCCA 2 cut(s) 745, 1303
MluCI AATT 5 cut(s) 283, 517, 775, 989, 1004
MluNI TGGCCA 2 cut(s) 745, 1303
MlyI GAGTC 2 cut(s) 113, 236
MmeI TCCRAC 4 cut(s) 9, 133, 506, 1232
Mox20I TGGCCA 2 cut(s) 745, 1303
MroXI GAANNNNTTC 1 cut(s) 333
MscI TGGCCA 2 cut(s) 745, 1303
MseI TTAA 1 cut(s) 864
Msp20I TGGCCA 2 cut(s) 745, 1303
MspI CCGG 1 cut(s) 91
MspR9I CCNGG 7 cut(s) 68, 92, 200, 677, 747, 762, 1315
MunI CAATTG 1 cut(s) 989
Mva1269I GAATGC 2 cut(s) 216, 901
MvaI CCWGG 6 cut(s) 68, 200, 677, 747, 762, 1315
MvnI CGCG 3 cut(s) 327, 339, 902
MwoI GCNNNNNNNGC 6 cut(s) 42, 45, 258, 451, 487, 940
NciI CCSGG 1 cut(s) 92
NcoI CCATGG 1 cut(s) 40
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 310, 658
NlaIII CATG 5 cut(s) 44, 347, 413, 1127, 1282
NlaIV GGNNCC 5 cut(s) 49, 184, 237, 942, 1119
NspI RCATGY 1 cut(s) 1127
PaeR7I CTCGAG 1 cut(s) 710
PagI TCATGA 1 cut(s) 1278
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PctI GAATGC 2 cut(s) 216, 901
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 370
PflFI GACNNNGTC 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 752
PfoI TCCNGGA 2 cut(s) 66, 675
PleI GAGTC 2 cut(s) 112, 236
PpsI GAGTC 2 cut(s) 112, 236
Psp124BI GAGCTC 3 cut(s) 447, 687, 739
Psp6I CCWGG 6 cut(s) 66, 198, 675, 745, 760, 1313
PspGI CCWGG 6 cut(s) 66, 198, 675, 745, 760, 1313
PspN4I GGNNCC 5 cut(s) 49, 184, 237, 942, 1119
PspPI GGNCC 3 cut(s) 76, 235, 1333
PspXI VCTCGAGB 1 cut(s) 710
PstI CTGCAG 2 cut(s) 483, 799
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 3 cut(s) 618, 803, 1010
RsaNI GTAC 3 cut(s) 617, 802, 1009
SacI GAGCTC 3 cut(s) 447, 687, 739
SaqAI TTAA 1 cut(s) 864
Sau3AI GATC 2 cut(s) 310, 658
Sau96I GGNCC 3 cut(s) 76, 235, 1333
SchI GAGTC 2 cut(s) 113, 236
ScrFI CCNGG 7 cut(s) 68, 92, 200, 677, 747, 762, 1315
SduI GDGCHC 4 cut(s) 447, 687, 739, 1275
SexAI ACCWGGT 1 cut(s) 198
SfaNI GCATC 4 cut(s) 463, 967, 1194, 1329
SfcI CTRYAG 2 cut(s) 479, 795
Sfr274I CTCGAG 1 cut(s) 710
SlaI CTCGAG 1 cut(s) 710
SmlI CTYRAG 2 cut(s) 221, 710
SmoI CTYRAG 2 cut(s) 221, 710
Sse9I AATT 5 cut(s) 283, 517, 775, 989, 1004
SstI GAGCTC 3 cut(s) 447, 687, 739
StyD4I CCNGG 7 cut(s) 66, 90, 198, 675, 745, 760, 1313
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 24, 571
TaqI TCGA 4 cut(s) 192, 608, 711, 930
TasI AATT 5 cut(s) 283, 517, 775, 989, 1004
TatI WGTACW 2 cut(s) 801, 1008
TauI GCSGC 6 cut(s) 48, 261, 327, 509, 902, 1123
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 864
Tru9I TTAA 1 cut(s) 864
TscAI CASTG 6 cut(s) 548, 576, 630, 661, 804, 954
TseI GCWGC 6 cut(s) 36, 356, 478, 481, 794, 995
TspDTI ATGAA 4 cut(s) 276, 788, 936, 1295
TspGWI ACGGA 2 cut(s) 97, 920
TspRI CASTG 6 cut(s) 548, 576, 630, 661, 804, 954
Tth111I GACNNNGTC 1 cut(s) 245
Van91I CCANNNNNTGG 1 cut(s) 752
XagI CCTNNNNNAGG 2 cut(s) 318, 1280
XapI RAATTY 1 cut(s) 775
XceI RCATGY 1 cut(s) 1127
XcmI CCANNNNNNNNNTGG 2 cut(s) 787, 1311
XhoI CTCGAG 1 cut(s) 710
XmnI GAANNNNTTC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.