Rw4G037600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011357
Physical Location & Seq
Reverse (-)
64126574 .. 64128303
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037600.1

Sequence Viewer

Length: 1203 bp
ATGGGAGAGCTGTTAGAGTCTGCCCGTCCATTCTTGCGCGGTGAGCTGGAGTCGATCGACAAGAATCTGCCTTCACTTGTGAAAGTGTTGCAGTCTGTTGGAGCCGGAGAGTGCTGGCATAAGCACGGCACTTTTCTGGAGCACCTGGTCGACATCTACCGCATTCTCAAAATATGGAAGGCCCCTGATTCCGTCTGCCTCTGCGGCCTCTTCCACTCCGCTTACTCCAATTCCTATGTCAACCTCGCCATATTCGACCCTTCCACCGGCCGCGACGTCGTTCGCCGCCATGTCGGGGAGGCTGCCGAGCGCTTGATCCACTTGTTCTGCATCGTCCCAAGGCAGCCACTCATCCACGATGACCTTCTCTTCCACTACACCGACGACGAGCTCCGCGACCATCTCAAGCAATCCGAAGCGGCTGTTTCTGGAGATTCTGGCAACTGCAACTGGAGGAAGAAAGTGCAGAGTCTGGTTCCTGCTGATGGGATCAGAGTGAAGCACATAAAGACGGGCGAAGATGTGTTGGTTTCGAGGAGGATCGTCGCGACGTTTGTGATGATGACCATGGCAGATTTCAGCGACCAACTGTTTGGTTTCCAGGACGCGCTGTTTGACAACGGAGACGGGCGGCTCGAGTTCACTGGGAACAAGTATGGAGCTCTGTGGCCTGGCGATGGCAAGCCGGGGCTCTGGATGAACTCCATTTCAAGGATGGCTGCACTCTACACACTCCTCGTCAGGGAGGAACAAGTGTTTATTCGGGAGAGAGGTGACGCCAACCTTAACATTTCTCGAGATGAAAATCTCCAACTCGTGGTGCCGCCAGTGTTTGACAACTGCACCAGGGTTTTGGATGCTGGGGAGCAATTAGCTGCCAGGGACCTCTATTGGGAAGCTGTCTGTGATTCTGATTCCGACGATTCTGCTTCACTGCTCAAGAGTTGCATTGAGAAAAACCCGTTCATCGGAGAGCCGCATGTGGTGTTGGGTCAGGTTTATTTGACGCAGGGAAAGTATGAGGAGGCTGAGAGACAAGCTGAGACAGGGCTGAGGCTTCTGCTGGAATGGGCGAGTTCTTGGGACAAGAGGATGTCTTGGCAAGGATGGATTGCTTGGGCCAGGGTTCTGCTGATGAAGGCCCGAGAAAGATCATGGCCTCAAACTTCCTGGGGCATTCTCAACTTGGGCCTCGTCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

45.32

Weight (kDa)

5.47

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6817 PF20680 29 - 114 7.2e-30 Domain of unknown function (DUF6817)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 820
AccB7I CCANNNNNTGG 1 cut(s) 817
AccI GTMKAC 1 cut(s) 150
AccII CGCG 5 cut(s) 39, 273, 396, 548, 608
AclWI GGATC 3 cut(s) 310, 497, 548
AcoI YGGCCR 1 cut(s) 268
AcyI GRCGYC 2 cut(s) 276, 777
AfeI AGCGCT 1 cut(s) 311
AfiI CCNNNNNNNGG 9 cut(s) 266, 295, 485, 677, 711, 742, 817, 892, 968
AgsI TTSAA 1 cut(s) 711
AjnI CCWGG 7 cut(s) 144, 600, 670, 845, 878, 1121, 1169
AluBI AGCT 7 cut(s) 10, 46, 391, 662, 875, 899, 1040
AluI AGCT 7 cut(s) 10, 46, 391, 662, 875, 899, 1040
Alw21I GWGCWC 3 cut(s) 144, 393, 664
Alw26I GTCTC 3 cut(s) 618, 1027, 1037
AlwI GGATC 3 cut(s) 310, 497, 548
AlwNI CAGNNNCTG 1 cut(s) 472
Ama87I CYCGRG 3 cut(s) 635, 795, 1143
Aor51HI AGCGCT 1 cut(s) 311
AoxI GGCC 8 cut(s) 180, 205, 268, 668, 1119, 1140, 1157, 1189
ApeKI GCWGC 4 cut(s) 302, 343, 719, 875
ArsI GACNNNNNNTTYG 4 cut(s) 575, 596, 607, 628
AspLEI GCGC 3 cut(s) 39, 312, 610
AspS9I GGNCC 5 cut(s) 181, 883, 1119, 1141, 1189
AsuC2I CCSGG 1 cut(s) 687
AsuHPI GGTGA 2 cut(s) 53, 785
AvaI CYCGRG 3 cut(s) 635, 795, 1143
AvaII GGWCC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 820
BanII GRGCYC 3 cut(s) 393, 664, 693
BauI CACGAG 1 cut(s) 815
Bbv12I GWGCWC 3 cut(s) 144, 393, 664
BbvCI CCTCAGC 1 cut(s) 1052
BbvI GCAGC 4 cut(s) 289, 355, 706, 862
BccI CCATC 5 cut(s) 408, 479, 671, 709, 1101
BceAI ACGGC 1 cut(s) 142
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 7 cut(s) 146, 602, 672, 847, 880, 1123, 1171
BcnI CCSGG 1 cut(s) 687
BcoDI GTCTC 3 cut(s) 618, 1027, 1037
BfoI RGCGCY 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 204
Bme1390I CCNGG 8 cut(s) 146, 602, 672, 687, 847, 880, 1123, 1171
Bme18I GGWCC 1 cut(s) 883
BmeT110I CYCGRG 3 cut(s) 635, 795, 1143
BmgT120I GGNCC 5 cut(s) 181, 883, 1119, 1141, 1189
BmiI GGNNCC 5 cut(s) 103, 183, 477, 822, 884
BmrFI CCNGG 8 cut(s) 146, 602, 672, 687, 847, 880, 1123, 1171
BmrI ACTGGG 1 cut(s) 654
BmsI GCATC 2 cut(s) 339, 847
BmuI ACTGGG 1 cut(s) 654
BpmI CTGGAG 4 cut(s) 68, 158, 450, 472
Bpu10I CCTNAGC 1 cut(s) 1052
BpuEI CTTGAG 2 cut(s) 389, 923
BpuMI CCSGG 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 804
BsaHI GRCGYC 2 cut(s) 276, 777
BsaJI CCNNGG 7 cut(s) 338, 567, 686, 846, 879, 1122, 1170
Bsc4I CCNNNNNNNGG 9 cut(s) 266, 295, 485, 677, 711, 742, 817, 892, 968
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 455, 649, 827
Bse8I GATNNNNATC 1 cut(s) 804
BseBI CCWGG 7 cut(s) 146, 602, 672, 847, 880, 1123, 1171
BseDI CCNNGG 7 cut(s) 338, 567, 686, 846, 879, 1122, 1170
BseGI GGATG 6 cut(s) 351, 702, 720, 862, 1098, 1112
BseJI GATNNNNATC 1 cut(s) 804
BseLI CCNNNNNNNGG 9 cut(s) 266, 295, 485, 677, 711, 742, 817, 892, 968
BseMII CTCAG 3 cut(s) 1020, 1032, 1043
BseNI ACTGG 3 cut(s) 455, 649, 827
BseRI GAGGAG 3 cut(s) 550, 725, 1037
BseX3I CGGCCG 1 cut(s) 268
BseXI GCAGC 4 cut(s) 289, 355, 706, 862
BseYI CCCAGC 1 cut(s) 860
BsgI GTGCAG 3 cut(s) 485, 705, 826
Bsh1236I CGCG 5 cut(s) 39, 273, 396, 548, 608
Bsh1285I CGRYCG 2 cut(s) 57, 271
BshFI GGCC 8 cut(s) 182, 207, 270, 670, 1121, 1142, 1159, 1191
BshNI GGYRCC 1 cut(s) 820
BsiEI CGRYCG 2 cut(s) 57, 271
BsiHKAI GWGCWC 3 cut(s) 144, 393, 664
BsiHKCI CYCGRG 3 cut(s) 635, 795, 1143
BsiSI CCGG 3 cut(s) 105, 267, 686
BslFI GGGAC 3 cut(s) 320, 896, 1097
BslI CCNNNNNNNGG 9 cut(s) 266, 295, 485, 677, 711, 742, 817, 892, 968
BsmAI GTCTC 3 cut(s) 618, 1027, 1037
BsmBI CGTCTC 1 cut(s) 618
BsmFI GGGAC 3 cut(s) 320, 896, 1097
BsmI GAATGC 2 cut(s) 162, 1176
BsnI GGCC 8 cut(s) 182, 207, 270, 670, 1121, 1142, 1159, 1191
BsoBI CYCGRG 3 cut(s) 635, 795, 1143
Bsp1286I GDGCHC 4 cut(s) 144, 393, 664, 693
Bsp143I GATC 5 cut(s) 54, 315, 489, 540, 1151
Bsp19I CCATGG 1 cut(s) 567
Bsp68I TCGCGA 1 cut(s) 548
BspANI GGCC 8 cut(s) 182, 207, 270, 670, 1121, 1142, 1159, 1191
BspCNI CTCAG 3 cut(s) 1021, 1033, 1044
BspFNI CGCG 5 cut(s) 39, 273, 396, 548, 608
BspLI GGNNCC 5 cut(s) 103, 183, 477, 822, 884
BspPI GGATC 3 cut(s) 310, 497, 548
BspT107I GGYRCC 1 cut(s) 820
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 3 cut(s) 455, 649, 827
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 7 cut(s) 338, 567, 686, 846, 879, 1122, 1170
BssMI GATC 5 cut(s) 54, 315, 489, 540, 1151
BssNI GRCGYC 2 cut(s) 276, 777
BssSI CACGAG 1 cut(s) 815
BssT1I CCWWGG 2 cut(s) 338, 567
Bst2BI CACGAG 1 cut(s) 815
Bst2UI CCWGG 7 cut(s) 146, 602, 672, 847, 880, 1123, 1171
Bst4CI ACNGT 1 cut(s) 591
Bst6I CTCTTC 2 cut(s) 215, 374
BstACI GRCGYC 2 cut(s) 276, 777
BstC8I GCNNGC 2 cut(s) 116, 683
BstDEI CTNAG 3 cut(s) 1029, 1041, 1052
BstDSI CCRYGG 1 cut(s) 567
BstF5I GGATG 6 cut(s) 351, 702, 720, 862, 1098, 1112
BstFNI CGCG 5 cut(s) 39, 273, 396, 548, 608
BstH2I RGCGCY 1 cut(s) 313
BstHHI GCGC 3 cut(s) 39, 312, 610
BstKTI GATC 5 cut(s) 57, 318, 492, 543, 1154
BstMAI GTCTC 3 cut(s) 618, 1027, 1037
BstMBI GATC 5 cut(s) 54, 315, 489, 540, 1151
BstMCI CGRYCG 2 cut(s) 57, 271
BstMWI GCNNNNNNNGC 2 cut(s) 43, 204
BstNI CCWGG 7 cut(s) 146, 602, 672, 847, 880, 1123, 1171
BstNSI RCATGY 1 cut(s) 983
BstSCI CCNGG 8 cut(s) 144, 600, 670, 685, 845, 878, 1121, 1169
BstUI CGCG 5 cut(s) 39, 273, 396, 548, 608
BstV1I GCAGC 4 cut(s) 289, 355, 706, 862
BstXI CCANNNNNNTGG 2 cut(s) 593, 853
BstZI CGGCCG 1 cut(s) 268
BsuRI GGCC 8 cut(s) 182, 207, 270, 670, 1121, 1142, 1159, 1191
BtgI CCRYGG 1 cut(s) 567
BtgZI GCGATG 1 cut(s) 690
BtsCI GGATG 6 cut(s) 351, 702, 720, 862, 1098, 1112
BtsI GCAGTG 1 cut(s) 932
BtsIMutI CAGTG 3 cut(s) 642, 834, 932
BtuMI TCGCGA 1 cut(s) 548
Cac8I GCNNGC 2 cut(s) 116, 683
CaiI CAGNNNCTG 1 cut(s) 472
CfoI GCGC 3 cut(s) 39, 312, 610
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 5 cut(s) 181, 883, 1119, 1141, 1189
CseI GACGC 3 cut(s) 614, 785, 1015
CsiI ACCWGGT 1 cut(s) 144
CviAII CATG 4 cut(s) 290, 568, 980, 1155
DdeI CTNAG 3 cut(s) 1029, 1041, 1052
DpnI GATC 5 cut(s) 56, 317, 491, 542, 1153
DpnII GATC 5 cut(s) 54, 315, 489, 540, 1151
EaeI YGGCCR 1 cut(s) 268
EagI CGGCCG 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 215, 374
EarI CTCTTC 2 cut(s) 215, 374
Ecl136II GAGCTC 2 cut(s) 391, 662
EclXI CGGCCG 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 338, 567
Eco24I GRGCYC 3 cut(s) 393, 664, 693
Eco47I GGWCC 1 cut(s) 883
Eco47III AGCGCT 1 cut(s) 311
Eco52I CGGCCG 1 cut(s) 268
Eco53kI GAGCTC 2 cut(s) 391, 662
Eco88I CYCGRG 3 cut(s) 635, 795, 1143
EcoICRI GAGCTC 2 cut(s) 391, 662
EcoO109I RGGNCCY 2 cut(s) 181, 883
EcoRII CCWGG 7 cut(s) 144, 600, 670, 845, 878, 1121, 1169
EcoT14I CCWWGG 2 cut(s) 338, 567
EcoT38I GRGCYC 3 cut(s) 393, 664, 693
ErhI CCWWGG 2 cut(s) 338, 567
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 4 cut(s) 293, 571, 983, 1158
FaqI GGGAC 3 cut(s) 320, 896, 1097
FatI CATG 4 cut(s) 289, 567, 979, 1154
FblI GTMKAC 1 cut(s) 150
FokI GGATG 6 cut(s) 338, 709, 727, 869, 1105, 1119
FriOI GRGCYC 3 cut(s) 393, 664, 693
GlaI GCGC 3 cut(s) 38, 311, 609
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 4 cut(s) 68, 158, 450, 472
HaeII RGCGCY 1 cut(s) 313
HaeIII GGCC 8 cut(s) 182, 207, 270, 670, 1121, 1142, 1159, 1191
HapII CCGG 3 cut(s) 105, 267, 686
HgaI GACGC 3 cut(s) 614, 785, 1015
HhaI GCGC 3 cut(s) 39, 312, 610
Hin1I GRCGYC 2 cut(s) 276, 777
Hin1II CATG 4 cut(s) 293, 571, 983, 1158
Hin6I GCGC 3 cut(s) 37, 310, 608
HinP1I GCGC 3 cut(s) 37, 310, 608
HincII GTYRAC 2 cut(s) 151, 241
HindII GTYRAC 2 cut(s) 151, 241
HinfI GANTC 9 cut(s) 17, 50, 64, 188, 434, 469, 908, 914, 923
HpaII CCGG 3 cut(s) 105, 267, 686
HphI GGTGA 2 cut(s) 53, 785
Hpy166II GTNNAC 3 cut(s) 151, 241, 642
Hpy188I TCNGA 5 cut(s) 415, 494, 913, 919, 971
Hpy188III TCNNGA 8 cut(s) 137, 429, 547, 694, 764, 795, 797, 940
Hpy8I GTNNAC 3 cut(s) 151, 241, 642
Hpy99I CGWCG 8 cut(s) 278, 281, 386, 389, 548, 553, 923, 1199
HpyAV CCTTC 5 cut(s) 81, 172, 270, 374, 1132
HpyCH4III ACNGT 1 cut(s) 591
HpyCH4IV ACGT 2 cut(s) 276, 551
HpyCH4V TGCA 7 cut(s) 91, 330, 447, 466, 722, 843, 948
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 204
HpyF3I CTNAG 3 cut(s) 1029, 1041, 1052
HpySE526I ACGT 2 cut(s) 276, 551
Hsp92I GRCGYC 2 cut(s) 276, 777
Hsp92II CATG 4 cut(s) 293, 571, 983, 1158
HspAI GCGC 3 cut(s) 37, 310, 608
Kzo9I GATC 5 cut(s) 54, 315, 489, 540, 1151
LmnI GCTCC 5 cut(s) 101, 139, 396, 659, 865
Lsp1109I GCAGC 4 cut(s) 289, 355, 706, 862
LweI GCATC 2 cut(s) 339, 847
MabI ACCWGGT 1 cut(s) 144
MaeII ACGT 2 cut(s) 276, 551
MaeIII GTNAC 1 cut(s) 773
MalI GATC 5 cut(s) 56, 317, 491, 542, 1153
MboI GATC 5 cut(s) 54, 315, 489, 540, 1151
MboII GAAGA 4 cut(s) 202, 361, 469, 530
MhlI GDGCHC 4 cut(s) 144, 393, 664, 693
MluCI AATT 2 cut(s) 229, 869
MlyI GAGTC 3 cut(s) 26, 59, 478
MmeI TCCRAC 3 cut(s) 79, 835, 942
MseI TTAA 1 cut(s) 786
MspI CCGG 3 cut(s) 105, 267, 686
MspR9I CCNGG 8 cut(s) 146, 602, 672, 687, 847, 880, 1123, 1171
Mva1269I GAATGC 2 cut(s) 162, 1176
MvaI CCWGG 7 cut(s) 146, 602, 672, 847, 880, 1123, 1171
MvnI CGCG 5 cut(s) 39, 273, 396, 548, 608
MwoI GCNNNNNNNGC 2 cut(s) 43, 204
NciI CCSGG 1 cut(s) 687
NcoI CCATGG 1 cut(s) 567
NdeII GATC 5 cut(s) 54, 315, 489, 540, 1151
NlaIII CATG 4 cut(s) 293, 571, 983, 1158
NlaIV GGNNCC 5 cut(s) 103, 183, 477, 822, 884
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 773
NruI TCGCGA 1 cut(s) 548
NspI RCATGY 1 cut(s) 983
PaeR7I CTCGAG 2 cut(s) 635, 795
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 393, 633
PctI GAATGC 2 cut(s) 162, 1176
PfeI GAWTC 6 cut(s) 64, 188, 434, 908, 914, 923
PflMI CCANNNNNTGG 1 cut(s) 817
PfoI TCCNGGA 1 cut(s) 600
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 3 cut(s) 25, 58, 477
PpsI GAGTC 3 cut(s) 25, 58, 477
PpuMI RGGWCCY 1 cut(s) 883
Psp124BI GAGCTC 2 cut(s) 393, 664
Psp5II RGGWCCY 1 cut(s) 883
Psp6I CCWGG 7 cut(s) 144, 600, 670, 845, 878, 1121, 1169
PspFI CCCAGC 1 cut(s) 860
PspGI CCWGG 7 cut(s) 144, 600, 670, 845, 878, 1121, 1169
PspN4I GGNNCC 5 cut(s) 103, 183, 477, 822, 884
PspPI GGNCC 5 cut(s) 181, 883, 1119, 1141, 1189
PspPPI RGGWCCY 1 cut(s) 883
PspXI VCTCGAGB 1 cut(s) 635
PstNI CAGNNNCTG 1 cut(s) 472
PvuI CGATCG 1 cut(s) 57
RruI TCGCGA 1 cut(s) 548
SacI GAGCTC 2 cut(s) 393, 664
SalI GTCGAC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 786
Sau3AI GATC 5 cut(s) 54, 315, 489, 540, 1151
Sau96I GGNCC 5 cut(s) 181, 883, 1119, 1141, 1189
SchI GAGTC 3 cut(s) 26, 59, 478
ScrFI CCNGG 8 cut(s) 146, 602, 672, 687, 847, 880, 1123, 1171
SduI GDGCHC 4 cut(s) 144, 393, 664, 693
SexAI ACCWGGT 1 cut(s) 144
SfaNI GCATC 2 cut(s) 339, 847
Sfr274I CTCGAG 2 cut(s) 635, 795
SinI GGWCC 1 cut(s) 883
SlaI CTCGAG 2 cut(s) 635, 795
SmlI CTYRAG 4 cut(s) 404, 635, 795, 938
SmoI CTYRAG 4 cut(s) 404, 635, 795, 938
Sse9I AATT 2 cut(s) 229, 869
SstI GAGCTC 2 cut(s) 393, 664
StyD4I CCNGG 8 cut(s) 144, 600, 670, 685, 845, 878, 1121, 1169
StyI CCWWGG 2 cut(s) 338, 567
TaaI ACNGT 1 cut(s) 591
TaiI ACGT 2 cut(s) 279, 554
TaqI TCGA 8 cut(s) 53, 57, 150, 255, 533, 636, 796, 1197
TasI AATT 2 cut(s) 229, 869
TauI GCSGC 7 cut(s) 207, 273, 288, 422, 634, 826, 979
TfiI GAWTC 6 cut(s) 64, 188, 434, 908, 914, 923
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 3 cut(s) 649, 834, 939
TseFI GTSAC 1 cut(s) 773
TseI GCWGC 4 cut(s) 302, 343, 719, 875
Tsp45I GTSAC 1 cut(s) 773
TspDTI ATGAA 4 cut(s) 713, 816, 955, 1151
TspGWI ACGGA 2 cut(s) 181, 636
TspRI CASTG 3 cut(s) 649, 834, 939
Van91I CCANNNNNTGG 1 cut(s) 817
VpaK11BI GGWCC 1 cut(s) 883
XceI RCATGY 1 cut(s) 983
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XhoI CTCGAG 2 cut(s) 635, 795
XmiI GTMKAC 1 cut(s) 150
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.