Rh4BG448600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011357
Physical Location & Seq
Reverse (-)
58684644 .. 58685855
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG448600.1

Sequence Viewer

Length: 1212 bp
ATGGGAAAGCTGTTAGAGTCTGCCCGTCCATTCTTGCGCGGTGAGCTGGAGTCGATCGACAAGAATCTGCCTTCACTTGTGAAAGTGTTGCAGTCTGTTGGAGCCGGTGAGTGCTGGCATAAGCACGGCACTTTTCTGGAGCACCTGGTAGACATCTACCGCATTCTCAAAATATGGAAGGCCCCTGATTCCGTCTGCCTCTGCGGCCTCTTCCACTCCGCTTACTCCAATTCCTATGTCAACCTCGCCATATTCGACCCTTCCACCGGCCGCGACGTCGTTCGCCGCCATGTCGGGGAGGCTGCCGAGCGCTTGATCCACTTGTTCTGCATCGTCCCAAGGCAGCCACTCATCCACGATGACCTTCTCTTCCACTACACCGACGACGAGCTCCGCGACCATCTCAAGCAATCCCAAGCGGCGGCTGTTTCTGGAGATGGAGATTCTGATATGAATTGCAACTGGAGGAAGAAAGTGCAGAGTCTGGTTCCTGCTGATGGGATCAGAGTGAAGCACATAAAGACGGGCGAAGATGTGTTGGTTTCGAGGAGGATCGTCGCGACGTTTGTGATGATGACCATGGCAGATTTCAGCGACCAACTGTTTGGTTTCCAGGACGCGCTGTTTGACAACGGAGACGGGCGGCTCGAGTTCACTGGGAACAAGTATGGAGCTCTGTGGCCTGGCGATGGCAAGCCGGGGCTCTGGATGAATTCCATTTCAAGGATGGCTGCACTCTACACACTCCTCGTCAGGGAGGAACAAGTGTTTATTCGAGAGAGAGGTGACGCCAACCTTGACATTTCTCGAGATGAAAATATCCAACTCGTGGCGCCGCCAGTGTTTGAGAACTGCACCAGGGTTTTGGATGCTGGGGAGCAATTAGCTGCCAGGGACCTCTATTGGGAAGCTGTTTGTGATTCTGATTCCGATTCTGCTTCACTGCTCAAGAGTTGCATTGAGAAAAACCCTTTCATCGGAGAGCCGCATGTGGTGTTGGGTCAGGTTTATCTGACGCAGGGAAAGTATGAGGAGGCTGAGAGAGAAGCTGAGACAGGGCTGAGGCTTCTGCTGGAATGGGCAAGTTCTTGGGACAAGAGGATGTCTTGGCAAGGATGGATTGCTTGGGCCAGGGTTCTGCTGATGAAGGCCCAAGAAAGATCATGGCCTCAAACTTCCTGGGGCATTCTCAACTTGGGCCTCGTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

403

Amino Acids

45.59

Weight (kDa)

5.53

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6817 PF20680 29 - 114 7.3e-30 Domain of unknown function (DUF6817)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 832
AccB7I CCANNNNNTGG 1 cut(s) 829
AccI GTMKAC 1 cut(s) 150
AccII CGCG 5 cut(s) 39, 273, 396, 560, 620
AclWI GGATC 3 cut(s) 310, 509, 560
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 1 cut(s) 712
AcyI GRCGYC 3 cut(s) 276, 789, 833
AfeI AGCGCT 1 cut(s) 311
AgsI TTSAA 1 cut(s) 723
AjnI CCWGG 7 cut(s) 144, 612, 682, 857, 890, 1130, 1178
AluBI AGCT 7 cut(s) 10, 46, 391, 674, 887, 911, 1049
AluI AGCT 7 cut(s) 10, 46, 391, 674, 887, 911, 1049
Alw21I GWGCWC 3 cut(s) 144, 393, 676
Alw26I GTCTC 2 cut(s) 630, 1046
AlwI GGATC 3 cut(s) 310, 509, 560
AlwNI CAGNNNCTG 1 cut(s) 484
Ama87I CYCGRG 2 cut(s) 647, 807
Aor51HI AGCGCT 1 cut(s) 311
AoxI GGCC 8 cut(s) 180, 205, 268, 680, 1128, 1149, 1166, 1198
ApeKI GCWGC 4 cut(s) 302, 343, 731, 887
ApoI RAATTY 1 cut(s) 712
ArsI GACNNNNNNTTYG 4 cut(s) 587, 608, 619, 640
AspLEI GCGC 4 cut(s) 39, 312, 622, 835
AspS9I GGNCC 5 cut(s) 181, 895, 1128, 1150, 1198
AsuC2I CCSGG 1 cut(s) 699
AsuHPI GGTGA 3 cut(s) 53, 119, 797
AvaI CYCGRG 2 cut(s) 647, 807
AvaII GGWCC 1 cut(s) 895
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 3 cut(s) 393, 676, 705
BauI CACGAG 1 cut(s) 827
Bbv12I GWGCWC 3 cut(s) 144, 393, 676
BbvCI CCTCAGC 1 cut(s) 1061
BbvI GCAGC 4 cut(s) 289, 355, 718, 874
BccI CCATC 6 cut(s) 408, 431, 491, 683, 721, 1110
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 7 cut(s) 146, 614, 684, 859, 892, 1132, 1180
BcnI CCSGG 1 cut(s) 699
BcoDI GTCTC 2 cut(s) 630, 1046
BfoI RGCGCY 2 cut(s) 313, 836
BglI GCCNNNNNGGC 1 cut(s) 204
Bme1390I CCNGG 8 cut(s) 146, 614, 684, 699, 859, 892, 1132, 1180
Bme18I GGWCC 1 cut(s) 895
BmeT110I CYCGRG 2 cut(s) 647, 807
BmgT120I GGNCC 5 cut(s) 181, 895, 1128, 1150, 1198
BmiI GGNNCC 5 cut(s) 103, 183, 489, 834, 896
BmrFI CCNGG 8 cut(s) 146, 614, 684, 699, 859, 892, 1132, 1180
BmrI ACTGGG 1 cut(s) 666
BmsI GCATC 2 cut(s) 339, 859
BmuI ACTGGG 1 cut(s) 666
BpmI CTGGAG 4 cut(s) 68, 158, 453, 484
Bpu10I CCTNAGC 1 cut(s) 1061
BpuEI CTTGAG 2 cut(s) 389, 932
BpuMI CCSGG 1 cut(s) 699
BsaHI GRCGYC 3 cut(s) 276, 789, 833
BsaJI CCNNGG 7 cut(s) 338, 579, 698, 858, 891, 1131, 1179
Bse118I RCCGGY 2 cut(s) 104, 266
Bse1I ACTGG 3 cut(s) 467, 661, 839
BseBI CCWGG 7 cut(s) 146, 614, 684, 859, 892, 1132, 1180
BseDI CCNNGG 7 cut(s) 338, 579, 698, 858, 891, 1131, 1179
BseGI GGATG 6 cut(s) 351, 714, 732, 874, 1107, 1121
BseMII CTCAG 3 cut(s) 1029, 1041, 1052
BseNI ACTGG 3 cut(s) 467, 661, 839
BseRI GAGGAG 3 cut(s) 562, 737, 1046
BseX3I CGGCCG 1 cut(s) 268
BseXI GCAGC 4 cut(s) 289, 355, 718, 874
BseYI CCCAGC 1 cut(s) 872
BsgI GTGCAG 3 cut(s) 497, 717, 838
Bsh1236I CGCG 5 cut(s) 39, 273, 396, 560, 620
Bsh1285I CGRYCG 2 cut(s) 57, 271
BshFI GGCC 8 cut(s) 182, 207, 270, 682, 1130, 1151, 1168, 1200
BshNI GGYRCC 1 cut(s) 832
BsiEI CGRYCG 2 cut(s) 57, 271
BsiHKAI GWGCWC 3 cut(s) 144, 393, 676
BsiHKCI CYCGRG 2 cut(s) 647, 807
BsiSI CCGG 3 cut(s) 105, 267, 698
BslFI GGGAC 3 cut(s) 320, 908, 1106
BsmAI GTCTC 2 cut(s) 630, 1046
BsmBI CGTCTC 1 cut(s) 630
BsmFI GGGAC 3 cut(s) 320, 908, 1106
BsmI GAATGC 2 cut(s) 162, 1185
BsnI GGCC 8 cut(s) 182, 207, 270, 682, 1130, 1151, 1168, 1200
BsoBI CYCGRG 2 cut(s) 647, 807
Bsp1286I GDGCHC 4 cut(s) 144, 393, 676, 705
Bsp143I GATC 5 cut(s) 54, 315, 501, 552, 1160
Bsp19I CCATGG 1 cut(s) 579
Bsp68I TCGCGA 1 cut(s) 560
BspANI GGCC 8 cut(s) 182, 207, 270, 682, 1130, 1151, 1168, 1200
BspCNI CTCAG 3 cut(s) 1030, 1042, 1053
BspFNI CGCG 5 cut(s) 39, 273, 396, 560, 620
BspLI GGNNCC 5 cut(s) 103, 183, 489, 834, 896
BspPI GGATC 3 cut(s) 310, 509, 560
BspT107I GGYRCC 1 cut(s) 832
BsrFI RCCGGY 2 cut(s) 104, 266
BsrI ACTGG 3 cut(s) 467, 661, 839
BssAI RCCGGY 2 cut(s) 104, 266
BssECI CCNNGG 7 cut(s) 338, 579, 698, 858, 891, 1131, 1179
BssMI GATC 5 cut(s) 54, 315, 501, 552, 1160
BssNI GRCGYC 3 cut(s) 276, 789, 833
BssSI CACGAG 1 cut(s) 827
BssT1I CCWWGG 2 cut(s) 338, 579
Bst2BI CACGAG 1 cut(s) 827
Bst2UI CCWGG 7 cut(s) 146, 614, 684, 859, 892, 1132, 1180
Bst4CI ACNGT 1 cut(s) 603
Bst6I CTCTTC 2 cut(s) 215, 374
BstACI GRCGYC 3 cut(s) 276, 789, 833
BstC8I GCNNGC 2 cut(s) 116, 695
BstDEI CTNAG 3 cut(s) 1038, 1050, 1061
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 6 cut(s) 351, 714, 732, 874, 1107, 1121
BstFNI CGCG 5 cut(s) 39, 273, 396, 560, 620
BstH2I RGCGCY 2 cut(s) 313, 836
BstHHI GCGC 4 cut(s) 39, 312, 622, 835
BstKTI GATC 5 cut(s) 57, 318, 504, 555, 1163
BstMAI GTCTC 2 cut(s) 630, 1046
BstMBI GATC 5 cut(s) 54, 315, 501, 552, 1160
BstMCI CGRYCG 2 cut(s) 57, 271
BstMWI GCNNNNNNNGC 2 cut(s) 43, 204
BstNI CCWGG 7 cut(s) 146, 614, 684, 859, 892, 1132, 1180
BstNSI RCATGY 1 cut(s) 992
BstSCI CCNGG 8 cut(s) 144, 612, 682, 697, 857, 890, 1130, 1178
BstUI CGCG 5 cut(s) 39, 273, 396, 560, 620
BstV1I GCAGC 4 cut(s) 289, 355, 718, 874
BstXI CCANNNNNNTGG 2 cut(s) 605, 865
BstZI CGGCCG 1 cut(s) 268
BsuRI GGCC 8 cut(s) 182, 207, 270, 682, 1130, 1151, 1168, 1200
BtgI CCRYGG 1 cut(s) 579
BtgZI GCGATG 1 cut(s) 702
BtsCI GGATG 6 cut(s) 351, 714, 732, 874, 1107, 1121
BtsI GCAGTG 1 cut(s) 941
BtsIMutI CAGTG 3 cut(s) 654, 846, 941
BtuMI TCGCGA 1 cut(s) 560
Cac8I GCNNGC 2 cut(s) 116, 695
CaiI CAGNNNCTG 1 cut(s) 484
CfoI GCGC 4 cut(s) 39, 312, 622, 835
Cfr10I RCCGGY 2 cut(s) 104, 266
Cfr13I GGNCC 5 cut(s) 181, 895, 1128, 1150, 1198
CseI GACGC 3 cut(s) 626, 797, 1024
CsiI ACCWGGT 1 cut(s) 144
CviAII CATG 4 cut(s) 290, 580, 989, 1164
DdeI CTNAG 3 cut(s) 1038, 1050, 1061
DinI GGCGCC 1 cut(s) 834
DpnI GATC 5 cut(s) 56, 317, 503, 554, 1162
DpnII GATC 5 cut(s) 54, 315, 501, 552, 1160
EaeI YGGCCR 1 cut(s) 268
EagI CGGCCG 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 215, 374
EarI CTCTTC 2 cut(s) 215, 374
Ecl136II GAGCTC 2 cut(s) 391, 674
EclXI CGGCCG 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 338, 579
Eco24I GRGCYC 3 cut(s) 393, 676, 705
Eco47I GGWCC 1 cut(s) 895
Eco47III AGCGCT 1 cut(s) 311
Eco52I CGGCCG 1 cut(s) 268
Eco53kI GAGCTC 2 cut(s) 391, 674
Eco88I CYCGRG 2 cut(s) 647, 807
EcoICRI GAGCTC 2 cut(s) 391, 674
EcoO109I RGGNCCY 2 cut(s) 181, 895
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 7 cut(s) 144, 612, 682, 857, 890, 1130, 1178
EcoT14I CCWWGG 2 cut(s) 338, 579
EcoT38I GRGCYC 3 cut(s) 393, 676, 705
EgeI GGCGCC 1 cut(s) 834
EheI GGCGCC 1 cut(s) 834
ErhI CCWWGG 2 cut(s) 338, 579
Esp3I CGTCTC 1 cut(s) 630
FaeI CATG 4 cut(s) 293, 583, 992, 1167
FaqI GGGAC 3 cut(s) 320, 908, 1106
FatI CATG 4 cut(s) 289, 579, 988, 1163
FblI GTMKAC 1 cut(s) 150
FokI GGATG 6 cut(s) 338, 721, 739, 881, 1114, 1128
FriOI GRGCYC 3 cut(s) 393, 676, 705
GlaI GCGC 4 cut(s) 38, 311, 621, 834
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 4 cut(s) 68, 158, 453, 484
HaeII RGCGCY 2 cut(s) 313, 836
HaeIII GGCC 8 cut(s) 182, 207, 270, 682, 1130, 1151, 1168, 1200
HapII CCGG 3 cut(s) 105, 267, 698
HgaI GACGC 3 cut(s) 626, 797, 1024
HhaI GCGC 4 cut(s) 39, 312, 622, 835
Hin1I GRCGYC 3 cut(s) 276, 789, 833
Hin1II CATG 4 cut(s) 293, 583, 992, 1167
Hin6I GCGC 4 cut(s) 37, 310, 620, 833
HinP1I GCGC 4 cut(s) 37, 310, 620, 833
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 9 cut(s) 17, 50, 64, 188, 443, 481, 920, 926, 932
HpaII CCGG 3 cut(s) 105, 267, 698
HphI GGTGA 3 cut(s) 53, 119, 797
Hpy166II GTNNAC 3 cut(s) 151, 241, 654
Hpy188I TCNGA 6 cut(s) 448, 506, 925, 931, 980, 1014
Hpy188III TCNNGA 8 cut(s) 137, 432, 559, 706, 776, 807, 809, 949
Hpy8I GTNNAC 3 cut(s) 151, 241, 654
Hpy99I CGWCG 6 cut(s) 278, 281, 386, 389, 560, 565
HpyAV CCTTC 5 cut(s) 81, 172, 270, 374, 1141
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4IV ACGT 2 cut(s) 276, 563
HpyCH4V TGCA 7 cut(s) 91, 330, 459, 478, 734, 855, 957
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 204
HpyF3I CTNAG 3 cut(s) 1038, 1050, 1061
HpySE526I ACGT 2 cut(s) 276, 563
Hsp92I GRCGYC 3 cut(s) 276, 789, 833
Hsp92II CATG 4 cut(s) 293, 583, 992, 1167
HspAI GCGC 4 cut(s) 37, 310, 620, 833
KasI GGCGCC 1 cut(s) 832
Kzo9I GATC 5 cut(s) 54, 315, 501, 552, 1160
LmnI GCTCC 5 cut(s) 101, 139, 396, 671, 877
Lsp1109I GCAGC 4 cut(s) 289, 355, 718, 874
LweI GCATC 2 cut(s) 339, 859
MabI ACCWGGT 1 cut(s) 144
MaeII ACGT 2 cut(s) 276, 563
MaeIII GTNAC 1 cut(s) 785
MalI GATC 5 cut(s) 56, 317, 503, 554, 1162
MboI GATC 5 cut(s) 54, 315, 501, 552, 1160
MboII GAAGA 4 cut(s) 202, 361, 481, 542
MhlI GDGCHC 4 cut(s) 144, 393, 676, 705
MluCI AATT 4 cut(s) 229, 454, 712, 881
Mly113I GGCGCC 1 cut(s) 833
MlyI GAGTC 3 cut(s) 26, 59, 490
MmeI TCCRAC 2 cut(s) 79, 847
MspI CCGG 3 cut(s) 105, 267, 698
MspR9I CCNGG 8 cut(s) 146, 614, 684, 699, 859, 892, 1132, 1180
Mva1269I GAATGC 2 cut(s) 162, 1185
MvaI CCWGG 7 cut(s) 146, 614, 684, 859, 892, 1132, 1180
MvnI CGCG 5 cut(s) 39, 273, 396, 560, 620
MwoI GCNNNNNNNGC 2 cut(s) 43, 204
NarI GGCGCC 1 cut(s) 833
NciI CCSGG 1 cut(s) 699
NcoI CCATGG 1 cut(s) 579
NdeII GATC 5 cut(s) 54, 315, 501, 552, 1160
NlaIII CATG 4 cut(s) 293, 583, 992, 1167
NlaIV GGNNCC 5 cut(s) 103, 183, 489, 834, 896
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 785
NruI TCGCGA 1 cut(s) 560
NspI RCATGY 1 cut(s) 992
PaeR7I CTCGAG 2 cut(s) 647, 807
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 393, 645
PctI GAATGC 2 cut(s) 162, 1185
PfeI GAWTC 6 cut(s) 64, 188, 443, 920, 926, 932
PflMI CCANNNNNTGG 1 cut(s) 829
PfoI TCCNGGA 1 cut(s) 612
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 3 cut(s) 25, 58, 489
PluTI GGCGCC 1 cut(s) 836
PpsI GAGTC 3 cut(s) 25, 58, 489
PpuMI RGGWCCY 1 cut(s) 895
Psp124BI GAGCTC 2 cut(s) 393, 676
Psp5II RGGWCCY 1 cut(s) 895
Psp6I CCWGG 7 cut(s) 144, 612, 682, 857, 890, 1130, 1178
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 7 cut(s) 144, 612, 682, 857, 890, 1130, 1178
PspN4I GGNNCC 5 cut(s) 103, 183, 489, 834, 896
PspPI GGNCC 5 cut(s) 181, 895, 1128, 1150, 1198
PspPPI RGGWCCY 1 cut(s) 895
PspXI VCTCGAGB 1 cut(s) 647
PstNI CAGNNNCTG 1 cut(s) 484
PvuI CGATCG 1 cut(s) 57
RruI TCGCGA 1 cut(s) 560
SacI GAGCTC 2 cut(s) 393, 676
Sau3AI GATC 5 cut(s) 54, 315, 501, 552, 1160
Sau96I GGNCC 5 cut(s) 181, 895, 1128, 1150, 1198
SchI GAGTC 3 cut(s) 26, 59, 490
ScrFI CCNGG 8 cut(s) 146, 614, 684, 699, 859, 892, 1132, 1180
SduI GDGCHC 4 cut(s) 144, 393, 676, 705
SexAI ACCWGGT 1 cut(s) 144
SfaNI GCATC 2 cut(s) 339, 859
SfoI GGCGCC 1 cut(s) 834
Sfr274I CTCGAG 2 cut(s) 647, 807
SinI GGWCC 1 cut(s) 895
SlaI CTCGAG 2 cut(s) 647, 807
SmlI CTYRAG 4 cut(s) 404, 647, 807, 947
SmoI CTYRAG 4 cut(s) 404, 647, 807, 947
Sse9I AATT 4 cut(s) 229, 454, 712, 881
SspDI GGCGCC 1 cut(s) 832
SstI GAGCTC 2 cut(s) 393, 676
StyD4I CCNGG 8 cut(s) 144, 612, 682, 697, 857, 890, 1130, 1178
StyI CCWWGG 2 cut(s) 338, 579
TaaI ACNGT 1 cut(s) 603
TaiI ACGT 2 cut(s) 279, 566
TaqI TCGA 7 cut(s) 53, 57, 255, 545, 648, 775, 808
TasI AATT 4 cut(s) 229, 454, 712, 881
TauI GCSGC 8 cut(s) 207, 273, 288, 422, 425, 646, 838, 988
TfiI GAWTC 6 cut(s) 64, 188, 443, 920, 926, 932
TscAI CASTG 3 cut(s) 661, 846, 948
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 4 cut(s) 302, 343, 731, 887
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 5 cut(s) 467, 725, 828, 964, 1160
TspGWI ACGGA 2 cut(s) 181, 648
TspRI CASTG 3 cut(s) 661, 846, 948
Van91I CCANNNNNTGG 1 cut(s) 829
VpaK11BI GGWCC 1 cut(s) 895
XapI RAATTY 1 cut(s) 712
XceI RCATGY 1 cut(s) 992
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XhoI CTCGAG 2 cut(s) 647, 807
XmiI GTMKAC 1 cut(s) 150
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.