Rh4AG439500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011357
Physical Location & Seq
Reverse (-)
73103520 .. 73104734
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG439500.1

Sequence Viewer

Length: 1215 bp
ATGGGAAAGCTGTTAGAGTCTGCCCGTCCATTCTTGCGCGGTGAGCTGGAGTCGATCGACAAGAATCTGCCTTCACTAGTCAAAGTGTTGCAGTCTGTTGGAGCCGGAGAGTGCTGGCATAAGCACGGCACTTTTCTGGAGCACCTGGTAGACATCTACCGCATTCTCAAAATATGGAAGGCCCCTGATTCCGTCTGCCTCTGCGGCCTCTTCCACTCCGCTTACTCCAATTCCTATGTCAACCTCGCCATATTCGACCCTTCCACCGGCCGCGACGTCGTTCGCCGCCATGTCGGGGAGGCTGCCGAGCGCTTGATCCACTTGTTCTGCATCGTCCCAAGGCAGCCACTCATCCACGATGACCTTCTCTTCCACTACACCGACGACGAGCTCCGCGACCATCTCAAGCAATCCGAAGCAGCTGTTTCTGGAGATTCTGATGATAATTGCAACTGCAACTGGAGGAAGAAAGTGCAGAGTCTGGTTCCTGCTGATGGGATCAGAGTGAAGCACATAAAGACGGGCGAAGATGTGTTGGTTTCGAGGAGGATCGTCGCGACGTTTGTGATGATGACCATGGCAGATTTCAGCGACCAACTGTTTGGTTTCCAGGACGCGCTGTTTGACAACGGAGACGGGCGGCTCGAGTTCACTGGGAACAAGTATGGAGCTCTGTGGCCTGGCGATGGCAAGCCGGGGCTCTGGATGAACTCCATTTCAAGGATGGCTGCACTCTACACACTCCTCGTCAGGGAGGAACAAGTGTTTATTCGGGAGAGAGGAGGTGACGCCAACCTTAACATTTCTCGAGATGAAAATCTCCAACTCGTGGTGCCGCCAGTGTTTGAGAACTGCACCAGGGTTTTGGATGCTGGGGAGCAATTAGCTGCCAGGGACCTCTATTGGGAAGCTGTCTGTGATTCTGATTCCCACGATTCTGCTTCACTGCTCAAGAGTTGCATTGAGAAAAACCCTTTCATCGGAGAGCCGCATGTGGTGTTGGGTCAGGCTTATTTGACGCAGCGAAAGTATGAGGAGGCTGAGAGAGAAGCTGAGACAGGGCTGAGGCTTCTGCTGGAATGGGCGAGTTCTTGGGACAAGAGGATGTCTTGGCAAGGATGGATTGCTTGGGCCAGGGTTCTGCTGATGAAGGCCCGAGAAAGATCATGGCCTCAAACTTCCTGGGGCATTCTCAACTTGGGCCTCGTCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

45.87

Weight (kDa)

5.62

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6817 PF20680 29 - 114 7.3e-30 Domain of unknown function (DUF6817)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 832
AccB7I CCANNNNNTGG 1 cut(s) 829
AccI GTMKAC 1 cut(s) 150
AccII CGCG 5 cut(s) 39, 273, 396, 557, 617
AclWI GGATC 3 cut(s) 310, 506, 557
AcoI YGGCCR 1 cut(s) 268
AcyI GRCGYC 2 cut(s) 276, 789
AfeI AGCGCT 1 cut(s) 311
AfiI CCNNNNNNNGG 9 cut(s) 266, 295, 494, 686, 720, 751, 829, 904, 980
AgsI TTSAA 1 cut(s) 720
AhlI ACTAGT 1 cut(s) 76
AjnI CCWGG 7 cut(s) 144, 609, 679, 857, 890, 1133, 1181
AluBI AGCT 8 cut(s) 10, 46, 391, 422, 671, 887, 911, 1052
AluI AGCT 8 cut(s) 10, 46, 391, 422, 671, 887, 911, 1052
Alw21I GWGCWC 3 cut(s) 144, 393, 673
Alw26I GTCTC 2 cut(s) 627, 1049
AlwI GGATC 3 cut(s) 310, 506, 557
AlwNI CAGNNNCTG 1 cut(s) 481
Ama87I CYCGRG 3 cut(s) 644, 807, 1155
Aor51HI AGCGCT 1 cut(s) 311
AoxI GGCC 8 cut(s) 180, 205, 268, 677, 1131, 1152, 1169, 1201
ApeKI GCWGC 6 cut(s) 302, 343, 419, 728, 887, 1021
ArsI GACNNNNNNTTYG 4 cut(s) 584, 605, 616, 637
AspLEI GCGC 3 cut(s) 39, 312, 619
AspS9I GGNCC 5 cut(s) 181, 895, 1131, 1153, 1201
AsuC2I CCSGG 1 cut(s) 696
AsuHPI GGTGA 2 cut(s) 53, 797
AvaI CYCGRG 3 cut(s) 644, 807, 1155
AvaII GGWCC 1 cut(s) 895
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 3 cut(s) 393, 673, 702
BauI CACGAG 1 cut(s) 827
Bbv12I GWGCWC 3 cut(s) 144, 393, 673
BbvCI CCTCAGC 1 cut(s) 1064
BbvI GCAGC 6 cut(s) 289, 355, 431, 715, 874, 1033
BccI CCATC 5 cut(s) 408, 488, 680, 718, 1113
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 7 cut(s) 146, 611, 681, 859, 892, 1135, 1183
BcnI CCSGG 1 cut(s) 696
BcoDI GTCTC 2 cut(s) 627, 1049
BcuI ACTAGT 1 cut(s) 76
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 204
Bme1390I CCNGG 8 cut(s) 146, 611, 681, 696, 859, 892, 1135, 1183
Bme18I GGWCC 1 cut(s) 895
BmeT110I CYCGRG 3 cut(s) 644, 807, 1155
BmgT120I GGNCC 5 cut(s) 181, 895, 1131, 1153, 1201
BmiI GGNNCC 5 cut(s) 103, 183, 486, 834, 896
BmrFI CCNGG 8 cut(s) 146, 611, 681, 696, 859, 892, 1135, 1183
BmrI ACTGGG 1 cut(s) 663
BmsI GCATC 2 cut(s) 339, 859
BmuI ACTGGG 1 cut(s) 663
BpmI CTGGAG 4 cut(s) 68, 158, 450, 481
Bpu10I CCTNAGC 1 cut(s) 1064
BpuEI CTTGAG 2 cut(s) 389, 935
BpuMI CCSGG 1 cut(s) 696
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 2 cut(s) 276, 789
BsaJI CCNNGG 7 cut(s) 338, 576, 695, 858, 891, 1134, 1182
Bsc4I CCNNNNNNNGG 9 cut(s) 266, 295, 494, 686, 720, 751, 829, 904, 980
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 464, 658, 839
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 7 cut(s) 146, 611, 681, 859, 892, 1135, 1183
BseDI CCNNGG 7 cut(s) 338, 576, 695, 858, 891, 1134, 1182
BseGI GGATG 6 cut(s) 351, 711, 729, 874, 1110, 1124
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 9 cut(s) 266, 295, 494, 686, 720, 751, 829, 904, 980
BseMII CTCAG 3 cut(s) 1032, 1044, 1055
BseNI ACTGG 3 cut(s) 464, 658, 839
BseRI GAGGAG 4 cut(s) 559, 734, 795, 1049
BseX3I CGGCCG 1 cut(s) 268
BseXI GCAGC 6 cut(s) 289, 355, 431, 715, 874, 1033
BseYI CCCAGC 1 cut(s) 872
BsgI GTGCAG 3 cut(s) 494, 714, 838
Bsh1236I CGCG 5 cut(s) 39, 273, 396, 557, 617
Bsh1285I CGRYCG 2 cut(s) 57, 271
BshFI GGCC 8 cut(s) 182, 207, 270, 679, 1133, 1154, 1171, 1203
BshNI GGYRCC 1 cut(s) 832
BsiEI CGRYCG 2 cut(s) 57, 271
BsiHKAI GWGCWC 3 cut(s) 144, 393, 673
BsiHKCI CYCGRG 3 cut(s) 644, 807, 1155
BsiSI CCGG 3 cut(s) 105, 267, 695
BslFI GGGAC 3 cut(s) 320, 908, 1109
BslI CCNNNNNNNGG 9 cut(s) 266, 295, 494, 686, 720, 751, 829, 904, 980
BsmAI GTCTC 2 cut(s) 627, 1049
BsmBI CGTCTC 1 cut(s) 627
BsmFI GGGAC 3 cut(s) 320, 908, 1109
BsmI GAATGC 2 cut(s) 162, 1188
BsnI GGCC 8 cut(s) 182, 207, 270, 679, 1133, 1154, 1171, 1203
BsoBI CYCGRG 3 cut(s) 644, 807, 1155
Bsp1286I GDGCHC 4 cut(s) 144, 393, 673, 702
Bsp143I GATC 5 cut(s) 54, 315, 498, 549, 1163
Bsp19I CCATGG 1 cut(s) 576
Bsp68I TCGCGA 1 cut(s) 557
BspANI GGCC 8 cut(s) 182, 207, 270, 679, 1133, 1154, 1171, 1203
BspCNI CTCAG 3 cut(s) 1033, 1045, 1056
BspFNI CGCG 5 cut(s) 39, 273, 396, 557, 617
BspLI GGNNCC 5 cut(s) 103, 183, 486, 834, 896
BspPI GGATC 3 cut(s) 310, 506, 557
BspT107I GGYRCC 1 cut(s) 832
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 3 cut(s) 464, 658, 839
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 7 cut(s) 338, 576, 695, 858, 891, 1134, 1182
BssMI GATC 5 cut(s) 54, 315, 498, 549, 1163
BssNI GRCGYC 2 cut(s) 276, 789
BssSI CACGAG 1 cut(s) 827
BssT1I CCWWGG 2 cut(s) 338, 576
Bst2BI CACGAG 1 cut(s) 827
Bst2UI CCWGG 7 cut(s) 146, 611, 681, 859, 892, 1135, 1183
Bst4CI ACNGT 1 cut(s) 600
Bst6I CTCTTC 2 cut(s) 215, 374
BstACI GRCGYC 2 cut(s) 276, 789
BstC8I GCNNGC 2 cut(s) 116, 692
BstDEI CTNAG 3 cut(s) 1041, 1053, 1064
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 6 cut(s) 351, 711, 729, 874, 1110, 1124
BstFNI CGCG 5 cut(s) 39, 273, 396, 557, 617
BstH2I RGCGCY 1 cut(s) 313
BstHHI GCGC 3 cut(s) 39, 312, 619
BstKTI GATC 5 cut(s) 57, 318, 501, 552, 1166
BstMAI GTCTC 2 cut(s) 627, 1049
BstMBI GATC 5 cut(s) 54, 315, 498, 549, 1163
BstMCI CGRYCG 2 cut(s) 57, 271
BstMWI GCNNNNNNNGC 2 cut(s) 43, 204
BstNI CCWGG 7 cut(s) 146, 611, 681, 859, 892, 1135, 1183
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 8 cut(s) 144, 609, 679, 694, 857, 890, 1133, 1181
BstUI CGCG 5 cut(s) 39, 273, 396, 557, 617
BstV1I GCAGC 6 cut(s) 289, 355, 431, 715, 874, 1033
BstXI CCANNNNNNTGG 2 cut(s) 602, 865
BstZI CGGCCG 1 cut(s) 268
BsuRI GGCC 8 cut(s) 182, 207, 270, 679, 1133, 1154, 1171, 1203
BtgI CCRYGG 1 cut(s) 576
BtgZI GCGATG 1 cut(s) 699
BtsCI GGATG 6 cut(s) 351, 711, 729, 874, 1110, 1124
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 3 cut(s) 651, 846, 944
BtuMI TCGCGA 1 cut(s) 557
Cac8I GCNNGC 2 cut(s) 116, 692
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 3 cut(s) 39, 312, 619
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 5 cut(s) 181, 895, 1131, 1153, 1201
CseI GACGC 3 cut(s) 623, 797, 1027
CsiI ACCWGGT 1 cut(s) 144
CviAII CATG 4 cut(s) 290, 577, 992, 1167
DdeI CTNAG 3 cut(s) 1041, 1053, 1064
DpnI GATC 5 cut(s) 56, 317, 500, 551, 1165
DpnII GATC 5 cut(s) 54, 315, 498, 549, 1163
EaeI YGGCCR 1 cut(s) 268
EagI CGGCCG 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 215, 374
EarI CTCTTC 2 cut(s) 215, 374
Ecl136II GAGCTC 2 cut(s) 391, 671
EclXI CGGCCG 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 338, 576
Eco24I GRGCYC 3 cut(s) 393, 673, 702
Eco47I GGWCC 1 cut(s) 895
Eco47III AGCGCT 1 cut(s) 311
Eco52I CGGCCG 1 cut(s) 268
Eco53kI GAGCTC 2 cut(s) 391, 671
Eco88I CYCGRG 3 cut(s) 644, 807, 1155
EcoICRI GAGCTC 2 cut(s) 391, 671
EcoO109I RGGNCCY 2 cut(s) 181, 895
EcoRII CCWGG 7 cut(s) 144, 609, 679, 857, 890, 1133, 1181
EcoT14I CCWWGG 2 cut(s) 338, 576
EcoT38I GRGCYC 3 cut(s) 393, 673, 702
ErhI CCWWGG 2 cut(s) 338, 576
Esp3I CGTCTC 1 cut(s) 627
FaeI CATG 4 cut(s) 293, 580, 995, 1170
FaqI GGGAC 3 cut(s) 320, 908, 1109
FatI CATG 4 cut(s) 289, 576, 991, 1166
FblI GTMKAC 1 cut(s) 150
FokI GGATG 6 cut(s) 338, 718, 736, 881, 1117, 1131
FriOI GRGCYC 3 cut(s) 393, 673, 702
FspBI CTAG 1 cut(s) 77
GlaI GCGC 3 cut(s) 38, 311, 618
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 4 cut(s) 68, 158, 450, 481
HaeII RGCGCY 1 cut(s) 313
HaeIII GGCC 8 cut(s) 182, 207, 270, 679, 1133, 1154, 1171, 1203
HapII CCGG 3 cut(s) 105, 267, 695
HgaI GACGC 3 cut(s) 623, 797, 1027
HhaI GCGC 3 cut(s) 39, 312, 619
Hin1I GRCGYC 2 cut(s) 276, 789
Hin1II CATG 4 cut(s) 293, 580, 995, 1170
Hin6I GCGC 3 cut(s) 37, 310, 617
HinP1I GCGC 3 cut(s) 37, 310, 617
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 9 cut(s) 17, 50, 64, 188, 434, 478, 920, 926, 935
HpaII CCGG 3 cut(s) 105, 267, 695
HphI GGTGA 2 cut(s) 53, 797
Hpy166II GTNNAC 3 cut(s) 151, 241, 651
Hpy188I TCNGA 5 cut(s) 415, 439, 503, 925, 983
Hpy188III TCNNGA 8 cut(s) 137, 429, 556, 703, 773, 807, 809, 952
Hpy8I GTNNAC 3 cut(s) 151, 241, 651
Hpy99I CGWCG 7 cut(s) 278, 281, 386, 389, 557, 562, 1211
HpyAV CCTTC 5 cut(s) 81, 172, 270, 374, 1144
HpyCH4III ACNGT 1 cut(s) 600
HpyCH4IV ACGT 2 cut(s) 276, 560
HpyCH4V TGCA 8 cut(s) 91, 330, 450, 456, 475, 731, 855, 960
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 204
HpyF3I CTNAG 3 cut(s) 1041, 1053, 1064
HpySE526I ACGT 2 cut(s) 276, 560
Hsp92I GRCGYC 2 cut(s) 276, 789
Hsp92II CATG 4 cut(s) 293, 580, 995, 1170
HspAI GCGC 3 cut(s) 37, 310, 617
Kzo9I GATC 5 cut(s) 54, 315, 498, 549, 1163
LmnI GCTCC 5 cut(s) 101, 139, 396, 668, 877
Lsp1109I GCAGC 6 cut(s) 289, 355, 431, 715, 874, 1033
LweI GCATC 2 cut(s) 339, 859
MabI ACCWGGT 1 cut(s) 144
MaeI CTAG 1 cut(s) 77
MaeII ACGT 2 cut(s) 276, 560
MaeIII GTNAC 1 cut(s) 785
MalI GATC 5 cut(s) 56, 317, 500, 551, 1165
MboI GATC 5 cut(s) 54, 315, 498, 549, 1163
MboII GAAGA 4 cut(s) 202, 361, 478, 539
MhlI GDGCHC 4 cut(s) 144, 393, 673, 702
MluCI AATT 3 cut(s) 229, 445, 881
MlyI GAGTC 3 cut(s) 26, 59, 487
MmeI TCCRAC 2 cut(s) 79, 847
MseI TTAA 1 cut(s) 798
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 3 cut(s) 105, 267, 695
MspR9I CCNGG 8 cut(s) 146, 611, 681, 696, 859, 892, 1135, 1183
Mva1269I GAATGC 2 cut(s) 162, 1188
MvaI CCWGG 7 cut(s) 146, 611, 681, 859, 892, 1135, 1183
MvnI CGCG 5 cut(s) 39, 273, 396, 557, 617
MwoI GCNNNNNNNGC 2 cut(s) 43, 204
NciI CCSGG 1 cut(s) 696
NcoI CCATGG 1 cut(s) 576
NdeII GATC 5 cut(s) 54, 315, 498, 549, 1163
NlaIII CATG 4 cut(s) 293, 580, 995, 1170
NlaIV GGNNCC 5 cut(s) 103, 183, 486, 834, 896
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 785
NruI TCGCGA 1 cut(s) 557
NspI RCATGY 1 cut(s) 995
PaeR7I CTCGAG 2 cut(s) 644, 807
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 393, 642
PctI GAATGC 2 cut(s) 162, 1188
PfeI GAWTC 6 cut(s) 64, 188, 434, 920, 926, 935
PflMI CCANNNNNTGG 1 cut(s) 829
PfoI TCCNGGA 1 cut(s) 609
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 3 cut(s) 25, 58, 486
PpsI GAGTC 3 cut(s) 25, 58, 486
PpuMI RGGWCCY 1 cut(s) 895
Psp124BI GAGCTC 2 cut(s) 393, 673
Psp5II RGGWCCY 1 cut(s) 895
Psp6I CCWGG 7 cut(s) 144, 609, 679, 857, 890, 1133, 1181
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 7 cut(s) 144, 609, 679, 857, 890, 1133, 1181
PspN4I GGNNCC 5 cut(s) 103, 183, 486, 834, 896
PspPI GGNCC 5 cut(s) 181, 895, 1131, 1153, 1201
PspPPI RGGWCCY 1 cut(s) 895
PspXI VCTCGAGB 1 cut(s) 644
PstNI CAGNNNCTG 1 cut(s) 481
PvuI CGATCG 1 cut(s) 57
PvuII CAGCTG 1 cut(s) 422
RruI TCGCGA 1 cut(s) 557
SacI GAGCTC 2 cut(s) 393, 673
SaqAI TTAA 1 cut(s) 798
Sau3AI GATC 5 cut(s) 54, 315, 498, 549, 1163
Sau96I GGNCC 5 cut(s) 181, 895, 1131, 1153, 1201
SchI GAGTC 3 cut(s) 26, 59, 487
ScrFI CCNGG 8 cut(s) 146, 611, 681, 696, 859, 892, 1135, 1183
SduI GDGCHC 4 cut(s) 144, 393, 673, 702
SexAI ACCWGGT 1 cut(s) 144
SfaNI GCATC 2 cut(s) 339, 859
Sfr274I CTCGAG 2 cut(s) 644, 807
SinI GGWCC 1 cut(s) 895
SlaI CTCGAG 2 cut(s) 644, 807
SmlI CTYRAG 4 cut(s) 404, 644, 807, 950
SmoI CTYRAG 4 cut(s) 404, 644, 807, 950
SpeI ACTAGT 1 cut(s) 76
Sse9I AATT 3 cut(s) 229, 445, 881
SspMI CTAG 1 cut(s) 77
SstI GAGCTC 2 cut(s) 393, 673
StyD4I CCNGG 8 cut(s) 144, 609, 679, 694, 857, 890, 1133, 1181
StyI CCWWGG 2 cut(s) 338, 576
TaaI ACNGT 1 cut(s) 600
TaiI ACGT 2 cut(s) 279, 563
TaqI TCGA 7 cut(s) 53, 57, 255, 542, 645, 808, 1209
TasI AATT 3 cut(s) 229, 445, 881
TauI GCSGC 6 cut(s) 207, 273, 288, 643, 838, 991
TfiI GAWTC 6 cut(s) 64, 188, 434, 920, 926, 935
Tru1I TTAA 1 cut(s) 798
Tru9I TTAA 1 cut(s) 798
TscAI CASTG 3 cut(s) 658, 846, 951
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 6 cut(s) 302, 343, 419, 728, 887, 1021
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 4 cut(s) 722, 828, 967, 1163
TspGWI ACGGA 2 cut(s) 181, 645
TspRI CASTG 3 cut(s) 658, 846, 951
Van91I CCANNNNNTGG 1 cut(s) 829
VpaK11BI GGWCC 1 cut(s) 895
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XhoI CTCGAG 2 cut(s) 644, 807
XmiI GTMKAC 1 cut(s) 150
XspI CTAG 1 cut(s) 77
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.