MD16G1004500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011357
Physical Location & Seq
Reverse (-)
344135 .. 345356
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1004500.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGCCTTCTCTGGTGGCCGTGCTGCGCTCAGTTGGAGCTGGCGAGTGCTGGCACAAGCACGGCACCTTTCTCGAACACCTCGTCATTCGTGCCCATGTCGGGGAGGCTGCGGAACGCTTGATTTACTTGTTCTGCATTGTCCCCAGACAGCCTCTCATCCACGACGACCTTTTGTTCTACTTCATCGACGTGGATCTCGTGGAGCATCTCAAACAATCAGAGATGTCGCTGCAGCAGCTGCAGAACCAATCGGAGGCTGCAAGCCCGAATTGTAGTTGGAGGAACAAGGTGCAGAGTCTTGTTCCCGCAAATGGGGTTACAGTGAAGCACATAAAGACCGGGGAAGATGTGTTGGTATCGAGGAGGGTGGTTGCAGTGTTTGTCCTGATGACCATGGCAGATTTCAGTGATCAACTCTTCGGTTTCCAGGACGAGCTCTTTGATAACAAAGATGGCCGCCTCGAGTTTAGGGGCAACAATTATGGAGCTCTGTGGCCCCGCGATGGCATGCCAGGGCTTTGGATGAATTCCATATCAAGGATGGCTGCAATATACAATTTGATAGTGAGAGAGGAGGAGATGTTTATCCAAGAGAAGAAAACTGTTAGTACTGCTACTGCTGCTGCTGGAAGTGGAGGGGATGAGAATGCCGGGGTGGACAAATGTAGAGATGAAGACATCGAGCTGGTGGTGCCACCAGTGTTTGACAAATGCACGAGGGTTCTGGATGCGGAAGAGCATTTGGCTGCAAGGGAATTGAACTGGGAAGCTGACAGAGCTGAGGAGTTGCTGTTGAGGTGTGTTGAGAAGAACCCTGTCATTGGGGAGCCGCATGTGGTGTTGGGTCAGGTTTATTTGACAGAAGCAAGGTTTGAGGAGGCGGAGAGGGAGGCAGAGAGAGGGCTAACCCGGATGCTGGAATGGAGGAGTGCTTGGGACAAGAGGATGACTTGGGAAGGATGGATTGCTTGGGCAAGGGTGCTCCTCATGAAGGCAAAGGACAGAAGTTGGCCACAAACTTCCTGGGCCATCCTCAACTTGGGCCTTGTCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

352

Amino Acids

40.03

Weight (kDa)

5.27

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 691
AccII CGCG 1 cut(s) 501
AciI CCGC 7 cut(s) 110, 306, 457, 499, 731, 830, 881
AclWI GGATC 1 cut(s) 201
AcoI YGGCCR 3 cut(s) 15, 454, 1010
AcsI RAATTY 1 cut(s) 526
AfaI GTAC 1 cut(s) 610
AgsI TTSAA 1 cut(s) 760
AjiI CACGTC 1 cut(s) 190
AjnI CCWGG 3 cut(s) 426, 511, 1022
AluBI AGCT 7 cut(s) 38, 238, 436, 488, 685, 770, 779
AluI AGCT 7 cut(s) 38, 238, 436, 488, 685, 770, 779
Alw21I GWGCWC 3 cut(s) 438, 490, 984
AlwI GGATC 1 cut(s) 201
AlwNI CAGNNNCTG 1 cut(s) 238
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 6 cut(s) 15, 454, 494, 1010, 1026, 1042
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 4 cut(s) 66, 98, 422, 454
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 3 cut(s) 495, 1026, 1042
AsuC2I CCSGG 3 cut(s) 340, 652, 910
AvaI CYCGRG 1 cut(s) 461
BaeGI GKGCMC 1 cut(s) 94
BalI TGGCCA 1 cut(s) 1012
BanI GGYRCC 2 cut(s) 62, 691
BanII GRGCYC 2 cut(s) 438, 490
BauI CACGAG 2 cut(s) 197, 715
BbsI GAAGAC 1 cut(s) 681
Bbv12I GWGCWC 3 cut(s) 438, 490, 984
BbvCI CCTCAGC 1 cut(s) 780
BccI CCATC 5 cut(s) 446, 497, 535, 954, 1037
BceAI ACGGC 2 cut(s) 2, 76
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 3 cut(s) 428, 513, 1024
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 3 cut(s) 340, 652, 910
BfmI CTRYAG 2 cut(s) 230, 239
BmcAI AGTACT 1 cut(s) 610
Bme1390I CCNGG 6 cut(s) 340, 428, 513, 652, 910, 1024
BmeT110I CYCGRG 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 190
BmgT120I GGNCC 3 cut(s) 495, 1026, 1042
BmiI GGNNCC 4 cut(s) 64, 497, 693, 828
BmrFI CCNGG 6 cut(s) 340, 428, 513, 652, 910, 1024
BmrI ACTGGG 1 cut(s) 772
BmsI GCATC 3 cut(s) 214, 718, 903
BmuI ACTGGG 1 cut(s) 772
BpiI GAAGAC 1 cut(s) 681
Bpu10I CCTNAGC 1 cut(s) 780
BpuMI CCSGG 3 cut(s) 340, 652, 910
BsaBI GATNNNNATC 1 cut(s) 584
BsaJI CCNNGG 5 cut(s) 339, 393, 512, 651, 1023
BsaXI ACNNNNNCTCC 4 cut(s) 566, 596, 776, 806
Bse1I ACTGG 2 cut(s) 698, 767
Bse8I GATNNNNATC 1 cut(s) 584
BseBI CCWGG 3 cut(s) 428, 513, 1024
BseDI CCNNGG 5 cut(s) 339, 393, 512, 651, 1023
BseGI GGATG 9 cut(s) 156, 528, 546, 646, 733, 918, 951, 965, 1029
BseJI GATNNNNATC 1 cut(s) 584
BseMII CTCAG 2 cut(s) 42, 771
BseNI ACTGG 2 cut(s) 698, 767
BseRI GAGGAG 7 cut(s) 376, 587, 590, 797, 890, 940, 974
BseSI GKGCMC 1 cut(s) 94
BsgI GTGCAG 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 501
BshFI GGCC 6 cut(s) 17, 456, 496, 1012, 1028, 1044
BshNI GGYRCC 2 cut(s) 62, 691
BsiHKAI GWGCWC 3 cut(s) 438, 490, 984
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 339, 651, 910
BslFI GGGAC 2 cut(s) 125, 950
BsmFI GGGAC 2 cut(s) 125, 950
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 6 cut(s) 17, 456, 496, 1012, 1028, 1044
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 4 cut(s) 94, 438, 490, 984
Bsp143I GATC 2 cut(s) 193, 409
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 7 cut(s) 110, 306, 457, 499, 731, 830, 881
BspANI GGCC 6 cut(s) 17, 456, 496, 1012, 1028, 1044
BspCNI CTCAG 2 cut(s) 41, 772
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 1 cut(s) 987
BspLI GGNNCC 4 cut(s) 64, 497, 693, 828
BspMAI CTGCAG 2 cut(s) 234, 243
BspPI GGATC 1 cut(s) 201
BspQI GCTCTTC 1 cut(s) 729
BspT107I GGYRCC 2 cut(s) 62, 691
BsrI ACTGG 2 cut(s) 698, 767
BssECI CCNNGG 5 cut(s) 339, 393, 512, 651, 1023
BssMI GATC 2 cut(s) 193, 409
BssSI CACGAG 2 cut(s) 197, 715
BssT1I CCWWGG 1 cut(s) 393
Bst2BI CACGAG 2 cut(s) 197, 715
Bst2UI CCWGG 3 cut(s) 428, 513, 1024
Bst4CI ACNGT 2 cut(s) 322, 604
Bst6I CTCTTC 2 cut(s) 422, 729
BstAPI GCANNNNNTGC 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 40, 50, 262, 509
BstDEI CTNAG 2 cut(s) 28, 780
BstDSI CCRYGG 1 cut(s) 393
BstENI CCTNNNNNAGG 1 cut(s) 989
BstF5I GGATG 9 cut(s) 156, 528, 546, 646, 733, 918, 951, 965, 1029
BstFNI CGCG 1 cut(s) 501
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 2 cut(s) 196, 412
BstMBI GATC 2 cut(s) 193, 409
BstMWI GCNNNNNNNGC 5 cut(s) 235, 238, 620, 691, 776
BstNI CCWGG 3 cut(s) 428, 513, 1024
BstNSI RCATGY 2 cut(s) 511, 836
BstSCI CCNGG 6 cut(s) 338, 426, 511, 650, 908, 1022
BstSFI CTRYAG 2 cut(s) 230, 239
BstSLI GKGCMC 1 cut(s) 94
BstUI CGCG 1 cut(s) 501
BstV2I GAAGAC 1 cut(s) 681
BstX2I RGATCY 1 cut(s) 193
BstXI CCANNNNNNTGG 1 cut(s) 519
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 6 cut(s) 17, 456, 496, 1012, 1028, 1044
BtgI CCRYGG 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 516
BtrI CACGTC 1 cut(s) 190
BtsCI GGATG 9 cut(s) 156, 528, 546, 646, 733, 918, 951, 965, 1029
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 4 cut(s) 327, 381, 412, 705
Cac8I GCNNGC 4 cut(s) 40, 50, 262, 509
CaiI CAGNNNCTG 1 cut(s) 238
CciI TCATGA 1 cut(s) 987
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 3 cut(s) 495, 1026, 1042
Csp6I GTAC 1 cut(s) 609
CviAII CATG 5 cut(s) 95, 394, 508, 833, 988
CviQI GTAC 1 cut(s) 609
DdeI CTNAG 2 cut(s) 28, 780
DpnI GATC 2 cut(s) 195, 411
DpnII GATC 2 cut(s) 193, 409
EaeI YGGCCR 3 cut(s) 15, 454, 1010
Eam1104I CTCTTC 2 cut(s) 422, 729
EarI CTCTTC 2 cut(s) 422, 729
EciI GGCGGA 1 cut(s) 896
Ecl136II GAGCTC 2 cut(s) 436, 488
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 2 cut(s) 438, 490
Eco53kI GAGCTC 2 cut(s) 436, 488
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 2 cut(s) 436, 488
EcoNI CCTNNNNNAGG 1 cut(s) 989
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 3 cut(s) 426, 511, 1022
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 2 cut(s) 438, 490
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 5 cut(s) 98, 397, 511, 836, 991
FaiI YATR 9 cut(s) 96, 332, 395, 483, 509, 533, 553, 834, 989
FaqI GGGAC 2 cut(s) 125, 950
FatI CATG 5 cut(s) 94, 393, 507, 832, 987
FauI CCCGC 2 cut(s) 313, 506
FbaI TGATCA 1 cut(s) 409
FokI GGATG 9 cut(s) 143, 535, 553, 653, 740, 925, 958, 972, 1016
FriOI GRGCYC 2 cut(s) 438, 490
GlaI GCGC 1 cut(s) 26
HaeIII GGCC 6 cut(s) 17, 456, 496, 1012, 1028, 1044
HapII CCGG 3 cut(s) 339, 651, 910
HhaI GCGC 1 cut(s) 27
Hin1II CATG 5 cut(s) 98, 397, 511, 836, 991
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HinfI GANTC 1 cut(s) 295
HpaII CCGG 3 cut(s) 339, 651, 910
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 2 cut(s) 220, 253
Hpy188III TCNNGA 4 cut(s) 71, 385, 725, 988
Hpy8I GTNNAC 1 cut(s) 658
Hpy99I CGWCG 2 cut(s) 167, 191
HpyAV CCTTC 3 cut(s) 15, 950, 985
HpyCH4III ACNGT 2 cut(s) 322, 604
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 9 cut(s) 135, 232, 241, 260, 292, 374, 548, 714, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 235, 238, 620, 691, 776
HpyF3I CTNAG 2 cut(s) 28, 780
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 5 cut(s) 98, 397, 511, 836, 991
HspAI GCGC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 2 cut(s) 193, 409
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 5 cut(s) 35, 202, 485, 826, 987
LweI GCATC 3 cut(s) 214, 718, 903
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 316
MalI GATC 2 cut(s) 195, 411
MboI GATC 2 cut(s) 193, 409
MboII GAAGA 6 cut(s) 356, 409, 607, 686, 746, 820
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 4 cut(s) 94, 438, 490, 984
MlsI TGGCCA 1 cut(s) 1012
MluCI AATT 5 cut(s) 268, 478, 526, 556, 755
MluNI TGGCCA 1 cut(s) 1012
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 2 cut(s) 13, 257
Mox20I TGGCCA 1 cut(s) 1012
MscI TGGCCA 1 cut(s) 1012
MslI CAYNNNNRTG 1 cut(s) 188
Msp20I TGGCCA 1 cut(s) 1012
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 3 cut(s) 339, 651, 910
MspR9I CCNGG 6 cut(s) 340, 428, 513, 652, 910, 1024
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 3 cut(s) 428, 513, 1024
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 5 cut(s) 235, 238, 620, 691, 776
NciI CCSGG 3 cut(s) 340, 652, 910
NcoI CCATGG 1 cut(s) 393
NdeII GATC 2 cut(s) 193, 409
NlaIII CATG 5 cut(s) 98, 397, 511, 836, 991
NlaIV GGNNCC 4 cut(s) 64, 497, 693, 828
NspI RCATGY 2 cut(s) 511, 836
PaeI GCATGC 1 cut(s) 511
PaeR7I CTCGAG 1 cut(s) 461
PagI TCATGA 1 cut(s) 987
PciSI GCTCTTC 1 cut(s) 729
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 195
PctI GAATGC 1 cut(s) 652
PfoI TCCNGGA 1 cut(s) 426
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
Psp124BI GAGCTC 2 cut(s) 438, 490
Psp6I CCWGG 3 cut(s) 426, 511, 1022
PspGI CCWGG 3 cut(s) 426, 511, 1022
PspN4I GGNNCC 4 cut(s) 64, 497, 693, 828
PspPI GGNCC 3 cut(s) 495, 1026, 1042
PspXI VCTCGAGB 1 cut(s) 461
PstI CTGCAG 2 cut(s) 234, 243
PstNI CAGNNNCTG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 610
RsaNI GTAC 1 cut(s) 609
RseI CAYNNNNRTG 1 cut(s) 188
SacI GAGCTC 2 cut(s) 438, 490
SapI GCTCTTC 1 cut(s) 729
Sau3AI GATC 2 cut(s) 193, 409
Sau96I GGNCC 3 cut(s) 495, 1026, 1042
ScaI AGTACT 1 cut(s) 610
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 6 cut(s) 340, 428, 513, 652, 910, 1024
SduI GDGCHC 4 cut(s) 94, 438, 490, 984
SfaNI GCATC 3 cut(s) 214, 718, 903
SfcI CTRYAG 2 cut(s) 230, 239
Sfr274I CTCGAG 1 cut(s) 461
SlaI CTCGAG 1 cut(s) 461
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
SphI GCATGC 1 cut(s) 511
Sse9I AATT 5 cut(s) 268, 478, 526, 556, 755
SsiI CCGC 7 cut(s) 110, 306, 457, 499, 731, 830, 881
SstI GAGCTC 2 cut(s) 438, 490
StyD4I CCNGG 6 cut(s) 338, 426, 511, 650, 908, 1022
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 2 cut(s) 322, 604
TaiI ACGT 1 cut(s) 192
TaqI TCGA 5 cut(s) 72, 186, 359, 462, 681
TasI AATT 5 cut(s) 268, 478, 526, 556, 755
TatI WGTACW 1 cut(s) 608
TauI GCSGC 2 cut(s) 459, 832
TscAI CASTG 4 cut(s) 327, 381, 412, 705
TspDTI ATGAA 4 cut(s) 172, 539, 687, 1004
TspRI CASTG 4 cut(s) 327, 381, 412, 705
XagI CCTNNNNNAGG 1 cut(s) 989
XapI RAATTY 1 cut(s) 526
XceI RCATGY 2 cut(s) 511, 836
XcmI CCANNNNNNNNNTGG 3 cut(s) 538, 1020, 1036
XhoI CTCGAG 1 cut(s) 461
ZrmI AGTACT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.