MD13G1013400.v1.1

Short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015203
Physical Location & Seq
Forward (+)
844730 .. 845710
981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1013400.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGAGCAATCTCTTCCAGTGCACGATGAGGACGAAGCGCGGAGGCCGCCTTCAAAGAGGTTGGCGAACAAAGTTGCAGTCATAACTGGGGGTGCAAGAGGGATTGGAGCTGCCGCGGCCAAGCTATTCGCCCGAAACGGAGCCCATGTCATCGTTGCTGATATACTCGACGACATAGGAGCTACACTGGCTGATTCTATCGGAGGCCGCTACATACACTGCGATGTGTCAAAGGAAGCTGATGTTGAATCGGCTGTTGAGCTTGCACTCAAATGGAAAGGCCGAATTGACATCATGTTCAACAATGCCGGCGTTCCTGGTCCCTCGGGGAGTATATCCAGCCTTGACATGGAGCAGGTCAAGGACCTCCTCTCGATCAATGTTCTCGGCGTTGTGCACGGAATCAAGTACGCTGCAGCGGCCATGATCAAAGGTCGAAGAGGAGGGAGCATCATTTGCACATCAAGCTCAGCCGGTATCATGGGAGGCCTTGGGGGACATGCCTACACATTGTCGAAGGAGGCCCTCAACGGATTGGCAAAAAGTGCCGCGTGTGAGTTGGGAGTGCATGGCATTCGAGTGAACAACATTTCGCCTCACGGGGTTCCTTCGGAGATGCTCGTCGATGCCTACAGAATGCATCTCGGGAAAATGGACATGACGGCAGAAGATGTAAGCAGAATTGTGGGAGAGAGGGGGAGCTTGTTGCGCGGGAGGTGCGCAACGGTGGAAGATGTGGCGGAAGCCGCATTGTTCATGGCCAGCGAAGACGCCGGGTTTATAACGGGACATAATCTTGTTCTTGATGGGGGTTATACTTCTGCTTGCAGTACCATGAGCTTCATCTACCAGAACAGTAACAAGGATACAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

292

Amino Acids

30.39

Weight (kDa)

5.99

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 24 - 209 4.2e-46 short chain dehydrogenase
KR PF08659 27 - 183 2.2e-09 KR domain
adh_short_C2 PF13561 31 - 273 5.9e-54 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015203)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42960
fragaria_vesca FvH4_4g35960
malus_domestica MD13G1013400.v1.1 MD16G1010300.v1.1
prunus_persica Prupe.1G340300_v2.0.a1
pyrus_communis pycom16g01010
rosa_laevigata RLG00000005727
rosa_multiflora Rmu_ssc0000107.1_g000001 Rmu_ssc0000253.1_g000009
rosa_roxburghii Rroxscaffold_5G00386070
rosa_rugosa Rorug04G0362800
rosa_samantha Rh4AG424200 Rh4BG436300 Rh4CG450900 Rh4DG432800
rosa_wichuraiana Rw4G036370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
Acc16I TGCGCA 1 cut(s) 721
Acc36I ACCTGC 1 cut(s) 346
AccII CGCG 4 cut(s) 40, 116, 551, 711
AcoI YGGCCR 3 cut(s) 117, 420, 759
AcyI GRCGYC 1 cut(s) 771
AfaI GTAC 2 cut(s) 410, 832
AfiI CCNNNNNNNGG 1 cut(s) 349
AgsI TTSAA 3 cut(s) 54, 248, 301
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 8 cut(s) 110, 124, 182, 239, 262, 468, 702, 840
AluI AGCT 8 cut(s) 110, 124, 182, 239, 262, 468, 702, 840
Alw21I GWGCWC 2 cut(s) 24, 399
Alw44I GTGCAC 2 cut(s) 20, 395
Ama87I CYCGRG 2 cut(s) 325, 644
AoxI GGCC 8 cut(s) 44, 117, 205, 280, 420, 487, 522, 759
ApaLI GTGCAC 2 cut(s) 20, 395
ApeKI GCWGC 3 cut(s) 110, 413, 416
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
AspLEI GCGC 3 cut(s) 40, 711, 722
AspS9I GGNCC 3 cut(s) 320, 364, 523
AsuC2I CCSGG 1 cut(s) 775
AvaI CYCGRG 2 cut(s) 325, 644
AvaII GGWCC 2 cut(s) 320, 364
BaeGI GKGCMC 2 cut(s) 24, 399
BalI TGGCCA 1 cut(s) 761
BanII GRGCYC 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 774
Bbv12I GWGCWC 2 cut(s) 24, 399
BbvI GCAGC 3 cut(s) 97, 400, 428
BccI CCATC 1 cut(s) 800
BceAI ACGGC 1 cut(s) 678
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 1 cut(s) 318
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 1 cut(s) 426
BcnI CCSGG 1 cut(s) 775
BfmI CTRYAG 2 cut(s) 414, 631
BfuAI ACCTGC 1 cut(s) 346
BfuI GTATCC 1 cut(s) 859
BlpI GCTNAGC 1 cut(s) 469
Bme1390I CCNGG 2 cut(s) 318, 775
Bme18I GGWCC 2 cut(s) 320, 364
BmeT110I CYCGRG 2 cut(s) 325, 644
BmgT120I GGNCC 3 cut(s) 320, 364, 523
BmiI GGNNCC 3 cut(s) 142, 322, 606
BmrFI CCNGG 2 cut(s) 318, 775
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 4 cut(s) 459, 606, 616, 649
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 774
Bpu1102I GCTNAGC 1 cut(s) 469
BpuMI CCSGG 1 cut(s) 775
BsaHI GRCGYC 1 cut(s) 771
BsaJI CCNNGG 3 cut(s) 114, 324, 490
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse118I RCCGGY 2 cut(s) 308, 473
Bse1I ACTGG 3 cut(s) 17, 91, 192
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 3 cut(s) 114, 324, 490
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 3 cut(s) 17, 91, 192
BseRI GAGGAG 2 cut(s) 359, 456
BseSI GKGCMC 2 cut(s) 24, 399
BseXI GCAGC 3 cut(s) 97, 400, 428
Bsh1236I CGCG 4 cut(s) 40, 116, 551, 711
BshFI GGCC 8 cut(s) 46, 119, 207, 282, 422, 489, 524, 761
BsiHKAI GWGCWC 2 cut(s) 24, 399
BsiHKCI CYCGRG 2 cut(s) 325, 644
BsiSI CCGG 3 cut(s) 309, 474, 774
BslFI GGGAC 3 cut(s) 306, 510, 801
BslI CCNNNNNNNGG 1 cut(s) 349
BsmFI GGGAC 3 cut(s) 306, 510, 801
BsmI GAATGC 2 cut(s) 573, 642
BsnI GGCC 8 cut(s) 46, 119, 207, 282, 422, 489, 524, 761
BsoBI CYCGRG 2 cut(s) 325, 644
Bsp1286I GDGCHC 3 cut(s) 24, 145, 399
Bsp143I GATC 2 cut(s) 375, 426
Bsp1720I GCTNAGC 1 cut(s) 469
BspANI GGCC 8 cut(s) 46, 119, 207, 282, 422, 489, 524, 761
BspCNI CTCAG 1 cut(s) 482
BspFNI CGCG 4 cut(s) 40, 116, 551, 711
BspLI GGNNCC 3 cut(s) 142, 322, 606
BspMAI CTGCAG 1 cut(s) 418
BspMI ACCTGC 1 cut(s) 346
BsrFI RCCGGY 2 cut(s) 308, 473
BsrI ACTGG 3 cut(s) 17, 91, 192
BssAI RCCGGY 2 cut(s) 308, 473
BssECI CCNNGG 3 cut(s) 114, 324, 490
BssMI GATC 2 cut(s) 375, 426
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 490
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 727, 857, 871
Bst6I CTCTTC 2 cut(s) 18, 433
BstACI GRCGYC 1 cut(s) 771
BstAPI GCANNNNNTGC 2 cut(s) 456, 545
BstC8I GCNNGC 4 cut(s) 264, 310, 763, 826
BstDEI CTNAG 1 cut(s) 469
BstDSI CCRYGG 1 cut(s) 114
BstFNI CGCG 4 cut(s) 40, 116, 551, 711
BstHHI GCGC 3 cut(s) 40, 711, 722
BstKTI GATC 2 cut(s) 378, 429
BstMBI GATC 2 cut(s) 375, 426
BstNI CCWGG 1 cut(s) 318
BstNSI RCATGY 1 cut(s) 503
BstSCI CCNGG 2 cut(s) 316, 773
BstSFI CTRYAG 2 cut(s) 414, 631
BstSLI GKGCMC 2 cut(s) 24, 399
BstUI CGCG 4 cut(s) 40, 116, 551, 711
BstV1I GCAGC 3 cut(s) 97, 400, 428
BstV2I GAAGAC 1 cut(s) 774
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 8 cut(s) 46, 119, 207, 282, 422, 489, 524, 761
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 4 cut(s) 24, 185, 217, 876
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 4 cut(s) 264, 310, 763, 826
CfoI GCGC 3 cut(s) 40, 711, 722
Cfr10I RCCGGY 2 cut(s) 308, 473
Cfr13I GGNCC 3 cut(s) 320, 364, 523
Cfr42I CCGCGG 1 cut(s) 117
CseI GACGC 1 cut(s) 779
Csp6I GTAC 2 cut(s) 409, 831
CviQI GTAC 2 cut(s) 409, 831
DdeI CTNAG 1 cut(s) 469
DpnI GATC 2 cut(s) 377, 428
DpnII GATC 2 cut(s) 375, 426
EaeI YGGCCR 3 cut(s) 117, 420, 759
Eam1104I CTCTTC 2 cut(s) 18, 433
EarI CTCTTC 2 cut(s) 18, 433
EciI GGCGGA 1 cut(s) 755
Eco130I CCWWGG 1 cut(s) 490
Eco147I AGGCCT 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 320, 364
Eco88I CYCGRG 2 cut(s) 325, 644
EcoO109I RGGNCCY 2 cut(s) 364, 523
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 490
EcoT22I ATGCAT 1 cut(s) 642
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 490
FaqI GGGAC 3 cut(s) 306, 510, 801
FauI CCCGC 1 cut(s) 704
FbaI TGATCA 1 cut(s) 426
FriOI GRGCYC 1 cut(s) 145
FspI TGCGCA 1 cut(s) 721
GlaI GCGC 3 cut(s) 39, 710, 721
HaeIII GGCC 8 cut(s) 46, 119, 207, 282, 422, 489, 524, 761
HapII CCGG 3 cut(s) 309, 474, 774
HgaI GACGC 1 cut(s) 779
HhaI GCGC 3 cut(s) 40, 711, 722
Hin1I GRCGYC 1 cut(s) 771
Hin6I GCGC 3 cut(s) 38, 709, 720
HinP1I GCGC 3 cut(s) 38, 709, 720
HinfI GANTC 3 cut(s) 194, 248, 402
HpaII CCGG 3 cut(s) 309, 474, 774
Hpy166II GTNNAC 3 cut(s) 22, 397, 583
Hpy188I TCNGA 2 cut(s) 203, 613
Hpy188III TCNNGA 3 cut(s) 373, 646, 803
Hpy8I GTNNAC 3 cut(s) 22, 397, 583
Hpy99I CGWCG 2 cut(s) 173, 626
HpyAV CCTTC 3 cut(s) 60, 511, 618
HpyCH4III ACNGT 3 cut(s) 727, 857, 871
HpyF3I CTNAG 1 cut(s) 469
Hsp92I GRCGYC 1 cut(s) 771
HspAI GCGC 3 cut(s) 38, 709, 720
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 426
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 2 cut(s) 375, 426
LmnI GCTCC 7 cut(s) 4, 107, 140, 179, 352, 447, 699
Lsp1109I GCAGC 3 cut(s) 97, 400, 428
LweI GCATC 4 cut(s) 459, 606, 616, 649
MaeIII GTNAC 1 cut(s) 857
MalI GATC 2 cut(s) 377, 428
MboI GATC 2 cut(s) 375, 426
MboII GAAGA 5 cut(s) 5, 450, 680, 743, 779
MhlI GDGCHC 3 cut(s) 24, 145, 399
MlsI TGGCCA 1 cut(s) 761
MluCI AATT 2 cut(s) 285, 681
MluNI TGGCCA 1 cut(s) 761
Mox20I TGGCCA 1 cut(s) 761
Mph1103I ATGCAT 1 cut(s) 642
MroNI GCCGGC 1 cut(s) 308
MscI TGGCCA 1 cut(s) 761
MslI CAYNNNNRTG 3 cut(s) 222, 271, 578
Msp20I TGGCCA 1 cut(s) 761
MspA1I CMGCKG 2 cut(s) 116, 419
MspI CCGG 3 cut(s) 309, 474, 774
MspR9I CCNGG 2 cut(s) 318, 775
Mva1269I GAATGC 2 cut(s) 573, 642
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 4 cut(s) 40, 116, 551, 711
NaeI GCCGGC 1 cut(s) 310
NciI CCSGG 1 cut(s) 775
NdeII GATC 2 cut(s) 375, 426
NgoMIV GCCGGC 1 cut(s) 308
NlaIV GGNNCC 3 cut(s) 142, 322, 606
NmeAIII GCCGAG 1 cut(s) 366
NsbI TGCGCA 1 cut(s) 721
NsiI ATGCAT 1 cut(s) 642
NspI RCATGY 1 cut(s) 503
PceI AGGCCT 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 2 cut(s) 573, 642
PdiI GCCGGC 1 cut(s) 310
PfeI GAWTC 3 cut(s) 194, 248, 402
PpuMI RGGWCCY 1 cut(s) 364
PsiI TTATAA 1 cut(s) 782
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 142, 322, 606
PspPI GGNCC 3 cut(s) 320, 364, 523
PspPPI RGGWCCY 1 cut(s) 364
PstI CTGCAG 1 cut(s) 418
RsaI GTAC 2 cut(s) 410, 832
RsaNI GTAC 2 cut(s) 409, 831
RseI CAYNNNNRTG 3 cut(s) 222, 271, 578
SacII CCGCGG 1 cut(s) 117
Sau3AI GATC 2 cut(s) 375, 426
Sau96I GGNCC 3 cut(s) 320, 364, 523
ScrFI CCNGG 2 cut(s) 318, 775
SduI GDGCHC 3 cut(s) 24, 145, 399
SfaNI GCATC 4 cut(s) 459, 606, 616, 649
SfcI CTRYAG 2 cut(s) 414, 631
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SinI GGWCC 2 cut(s) 320, 364
SmiMI CAYNNNNRTG 3 cut(s) 222, 271, 578
Sse9I AATT 2 cut(s) 285, 681
SseBI AGGCCT 1 cut(s) 489
StuI AGGCCT 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 316, 773
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 3 cut(s) 727, 857, 871
TaqI TCGA 6 cut(s) 168, 374, 436, 515, 577, 624
TasI AATT 2 cut(s) 285, 681
TauI GCSGC 7 cut(s) 49, 116, 119, 210, 422, 551, 749
TfiI GAWTC 3 cut(s) 194, 248, 402
TscAI CASTG 4 cut(s) 24, 192, 224, 876
TseI GCWGC 3 cut(s) 110, 413, 416
TspDTI ATGAA 2 cut(s) 745, 832
TspGWI ACGGA 3 cut(s) 153, 414, 546
TspRI CASTG 4 cut(s) 24, 192, 224, 876
VneI GTGCAC 2 cut(s) 20, 395
VpaK11BI GGWCC 2 cut(s) 320, 364
XceI RCATGY 1 cut(s) 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
Zsp2I ATGCAT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.