MD16G1010300.v1.1

Short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015203
Physical Location & Seq
Forward (+)
810255 .. 811235
981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1010300.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGAGCCATCTATTCCGATGCACGATGAAGACGAAGCGCAGAGGCCGCCTTCAAAGAGGTTGGCGAACAAAGTTGCAGTCGTAACTGGGGGTGCAAGAGGGATTGGAGCTGCCACAGCCAAGCTACTCGCTCAACACGGAGCGCACGTCGTCGTTGCTGATATACTCGACGAGTTAGGCGCTACACTGGCTGATTCTATTGGCGGCCGCTACATACACTGTGATGTGTCAAAGGAAGCTGATGTTGAATCAGCTGTCGAGCTTGCACTCAAATGGAAAGGCCGACTTGACATCATGATCAACAATGCCGGTGTTCCCGGTCCCCCGGGGAGCATAACCACCCTCGACATGAAGCAGGTGATGCACCTTCTCTCAGTCAATGTTCTCGGGGCTGTGCATGGAATCAAGTACGCTGCAGCGGCCATGATCAACGGTCGAAGAGGAGGGAGCATCATTTGCACATCGAGCTCGGCCGGTATCATGGGAGGCCTTGGAGGACATGCCTACACATTGTCAAAGGCGGCCATCAACGGATTGGCAAGAAGTGCCGCGTGCGAGCTGGGAGTGCATGGCATTCGCGTGAACAACATTTCACCTCACGGGGTTCCTTCGGAGATGCTCGTCGATTCCTACAGATGGCATCTCGGGAAAATGGACATGACAGCAGAAGATGTAGGCAGAATTGTGGGAGAGAGGGGGAGCTCGTTGCGTGGTAGATGGGCGACTGTGGAGGATGTTGCAGAAGCTGCATTGTTCATGGCCAGCGAAGACGCCGGGTTTATAACCGGACATAATCTCGTTCTTGATGGGGGATATACTTCTGCTGTAAGTACCATGAGCTTCATCTACCAGAATAGAGATAAGGATACAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

292

Amino Acids

30.42

Weight (kDa)

6.11

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 24 - 210 2.8e-47 short chain dehydrogenase
KR PF08659 27 - 184 1.2e-09 KR domain
Epimerase PF01370 27 - 257 4.7e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 31 - 273 2.2e-54 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015203)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42960
fragaria_vesca FvH4_4g35960
malus_domestica MD13G1013400.v1.1 MD16G1010300.v1.1
prunus_persica Prupe.1G340300_v2.0.a1
pyrus_communis pycom16g01010
rosa_laevigata RLG00000005727
rosa_multiflora Rmu_ssc0000107.1_g000001 Rmu_ssc0000253.1_g000009
rosa_roxburghii Rroxscaffold_5G00386070
rosa_rugosa Rorug04G0362800
rosa_samantha Rh4AG424200 Rh4BG436300 Rh4CG450900 Rh4DG432800
rosa_wichuraiana Rw4G036370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 1 cut(s) 346
AccII CGCG 2 cut(s) 551, 579
AciI CCGC 6 cut(s) 47, 204, 208, 419, 521, 549
AcoI YGGCCR 5 cut(s) 205, 420, 471, 522, 759
AcyI GRCGYC 1 cut(s) 771
AfaI GTAC 2 cut(s) 410, 832
AfiI CCNNNNNNNGG 1 cut(s) 636
AgsI TTSAA 2 cut(s) 54, 248
AjiI CACGTC 1 cut(s) 148
Alw21I GWGCWC 2 cut(s) 470, 704
AlwNI CAGNNNCTG 1 cut(s) 746
Ama87I CYCGRG 3 cut(s) 325, 386, 644
AoxI GGCC 8 cut(s) 44, 205, 280, 420, 471, 487, 522, 759
ApeKI GCWGC 4 cut(s) 110, 413, 416, 746
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
AspLEI GCGC 3 cut(s) 40, 145, 182
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 4 cut(s) 318, 326, 327, 775
AsuHPI GGTGA 2 cut(s) 370, 585
AvaI CYCGRG 3 cut(s) 325, 386, 644
AvaII GGWCC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 761
BanII GRGCYC 2 cut(s) 470, 704
BbsI GAAGAC 2 cut(s) 36, 774
Bbv12I GWGCWC 2 cut(s) 470, 704
BbvI GCAGC 4 cut(s) 97, 400, 428, 733
BccI CCATC 5 cut(s) 16, 533, 630, 711, 800
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 2 cut(s) 297, 426
BcnI CCSGG 4 cut(s) 318, 326, 327, 775
BfmI CTRYAG 2 cut(s) 414, 631
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 346
BfuI GTATCC 1 cut(s) 859
Bme1390I CCNGG 4 cut(s) 318, 326, 327, 775
Bme18I GGWCC 1 cut(s) 320
BmeT110I CYCGRG 3 cut(s) 325, 386, 644
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 3 cut(s) 6, 322, 606
BmrFI CCNGG 4 cut(s) 318, 326, 327, 775
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 5 cut(s) 9, 351, 459, 606, 649
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 2 cut(s) 36, 774
BpuMI CCSGG 4 cut(s) 318, 326, 327, 775
BsaHI GRCGYC 1 cut(s) 771
BsaJI CCNNGG 4 cut(s) 324, 325, 326, 490
BsaWI WCCGGW 1 cut(s) 785
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 1 cut(s) 636
Bse118I RCCGGY 2 cut(s) 308, 473
Bse1I ACTGG 2 cut(s) 91, 192
BseDI CCNNGG 4 cut(s) 324, 325, 326, 490
BseGI GGATG 1 cut(s) 739
BseLI CCNNNNNNNGG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 91, 192
BseRI GAGGAG 1 cut(s) 456
BseX3I CGGCCG 2 cut(s) 205, 471
BseXI GCAGC 4 cut(s) 97, 400, 428, 733
BseYI CCCAGC 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 551, 579
Bsh1285I CGRYCG 3 cut(s) 208, 436, 474
BshFI GGCC 8 cut(s) 46, 207, 282, 422, 473, 489, 524, 761
BsiEI CGRYCG 3 cut(s) 208, 436, 474
BsiHKAI GWGCWC 2 cut(s) 470, 704
BsiHKCI CYCGRG 3 cut(s) 325, 386, 644
BsiSI CCGG 6 cut(s) 309, 318, 326, 474, 774, 786
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 636
BsmFI GGGAC 1 cut(s) 306
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 8 cut(s) 46, 207, 282, 422, 473, 489, 524, 761
BsoBI CYCGRG 3 cut(s) 325, 386, 644
Bsp1286I GDGCHC 2 cut(s) 470, 704
Bsp143I GATC 2 cut(s) 297, 426
BspACI CCGC 6 cut(s) 47, 204, 208, 419, 521, 549
BspANI GGCC 8 cut(s) 46, 207, 282, 422, 473, 489, 524, 761
BspCNI CTCAG 1 cut(s) 386
BspFNI CGCG 2 cut(s) 551, 579
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 6, 322, 606
BspMAI CTGCAG 1 cut(s) 418
BspMI ACCTGC 1 cut(s) 346
BsrFI RCCGGY 2 cut(s) 308, 473
BsrI ACTGG 2 cut(s) 91, 192
BssAI RCCGGY 2 cut(s) 308, 473
BssECI CCNNGG 4 cut(s) 324, 325, 326, 490
BssMI GATC 2 cut(s) 297, 426
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 490
Bst4CI ACNGT 3 cut(s) 221, 434, 727
Bst6I CTCTTC 1 cut(s) 433
BstACI GRCGYC 1 cut(s) 771
BstAPI GCANNNNNTGC 4 cut(s) 361, 456, 545, 746
BstC8I GCNNGC 4 cut(s) 264, 553, 557, 763
BstDEI CTNAG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 739
BstFNI CGCG 2 cut(s) 551, 579
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 3 cut(s) 40, 145, 182
BstKTI GATC 2 cut(s) 300, 429
BstMBI GATC 2 cut(s) 297, 426
BstMCI CGRYCG 3 cut(s) 208, 436, 474
BstNSI RCATGY 1 cut(s) 503
BstSCI CCNGG 4 cut(s) 316, 324, 325, 773
BstSFI CTRYAG 2 cut(s) 414, 631
BstUI CGCG 2 cut(s) 551, 579
BstV1I GCAGC 4 cut(s) 97, 400, 428, 733
BstV2I GAAGAC 2 cut(s) 36, 774
BstZI CGGCCG 2 cut(s) 205, 471
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 8 cut(s) 46, 207, 282, 422, 473, 489, 524, 761
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 1 cut(s) 739
BtsIMutI CAGTG 2 cut(s) 185, 217
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 4 cut(s) 264, 553, 557, 763
CaiI CAGNNNCTG 1 cut(s) 746
CciI TCATGA 1 cut(s) 294
CciNI GCGGCCGC 1 cut(s) 205
CfoI GCGC 3 cut(s) 40, 145, 182
Cfr10I RCCGGY 2 cut(s) 308, 473
Cfr13I GGNCC 1 cut(s) 320
Cfr9I CCCGGG 1 cut(s) 325
CseI GACGC 1 cut(s) 779
Csp6I GTAC 2 cut(s) 409, 831
CviQI GTAC 2 cut(s) 409, 831
DdeI CTNAG 1 cut(s) 373
DpnI GATC 2 cut(s) 299, 428
DpnII GATC 2 cut(s) 297, 426
EaeI YGGCCR 5 cut(s) 205, 420, 471, 522, 759
EagI CGGCCG 2 cut(s) 205, 471
Eam1104I CTCTTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 433
Ecl136II GAGCTC 2 cut(s) 468, 702
EclXI CGGCCG 2 cut(s) 205, 471
Eco130I CCWWGG 1 cut(s) 490
Eco147I AGGCCT 1 cut(s) 489
Eco24I GRGCYC 2 cut(s) 470, 704
Eco47I GGWCC 1 cut(s) 320
Eco52I CGGCCG 2 cut(s) 205, 471
Eco53kI GAGCTC 2 cut(s) 468, 702
Eco88I CYCGRG 3 cut(s) 325, 386, 644
EcoICRI GAGCTC 2 cut(s) 468, 702
EcoT14I CCWWGG 1 cut(s) 490
EcoT38I GRGCYC 2 cut(s) 470, 704
ErhI CCWWGG 1 cut(s) 490
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 1 cut(s) 306
FbaI TGATCA 2 cut(s) 297, 426
FokI GGATG 1 cut(s) 746
FriOI GRGCYC 2 cut(s) 470, 704
GlaI GCGC 3 cut(s) 39, 144, 181
GsaI CCCAGC 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 8 cut(s) 46, 207, 282, 422, 473, 489, 524, 761
HapII CCGG 6 cut(s) 309, 318, 326, 474, 774, 786
HgaI GACGC 1 cut(s) 779
HhaI GCGC 3 cut(s) 40, 145, 182
Hin1I GRCGYC 1 cut(s) 771
Hin6I GCGC 3 cut(s) 38, 143, 180
HinP1I GCGC 3 cut(s) 38, 143, 180
HinfI GANTC 4 cut(s) 194, 248, 402, 626
HpaII CCGG 6 cut(s) 309, 318, 326, 474, 774, 786
HphI GGTGA 2 cut(s) 370, 585
Hpy166II GTNNAC 1 cut(s) 583
Hpy188I TCNGA 2 cut(s) 18, 613
Hpy188III TCNNGA 3 cut(s) 295, 646, 803
Hpy8I GTNNAC 1 cut(s) 583
Hpy99I CGWCG 4 cut(s) 152, 155, 173, 626
HpyAV CCTTC 3 cut(s) 60, 377, 618
HpyCH4III ACNGT 3 cut(s) 221, 434, 727
HpyCH4IV ACGT 1 cut(s) 147
HpyF3I CTNAG 1 cut(s) 373
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 771
HspAI GCGC 3 cut(s) 38, 143, 180
Ksp22I TGATCA 2 cut(s) 297, 426
Kzo9I GATC 2 cut(s) 297, 426
LmnI GCTCC 6 cut(s) 4, 107, 140, 330, 447, 699
Lsp1109I GCAGC 4 cut(s) 97, 400, 428, 733
LweI GCATC 5 cut(s) 9, 351, 459, 606, 649
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 82
MalI GATC 2 cut(s) 299, 428
MboI GATC 2 cut(s) 297, 426
MboII GAAGA 4 cut(s) 41, 450, 680, 779
MhlI GDGCHC 2 cut(s) 470, 704
MlsI TGGCCA 1 cut(s) 761
MluCI AATT 1 cut(s) 681
MluNI TGGCCA 1 cut(s) 761
Mox20I TGGCCA 1 cut(s) 761
MscI TGGCCA 1 cut(s) 761
MslI CAYNNNNRTG 3 cut(s) 222, 271, 578
Msp20I TGGCCA 1 cut(s) 761
MspA1I CMGCKG 2 cut(s) 254, 419
MspI CCGG 6 cut(s) 309, 318, 326, 474, 774, 786
MspR9I CCNGG 4 cut(s) 318, 326, 327, 775
Mva1269I GAATGC 1 cut(s) 573
MvnI CGCG 2 cut(s) 551, 579
NciI CCSGG 4 cut(s) 318, 326, 327, 775
NdeII GATC 2 cut(s) 297, 426
NlaIV GGNNCC 3 cut(s) 6, 322, 606
NmeAIII GCCGAG 1 cut(s) 449
NotI GCGGCCGC 1 cut(s) 205
NspI RCATGY 1 cut(s) 503
PagI TCATGA 1 cut(s) 294
PaqCI CACCTGC 1 cut(s) 346
PceI AGGCCT 1 cut(s) 489
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 144
PctI GAATGC 1 cut(s) 573
PfeI GAWTC 4 cut(s) 194, 248, 402, 626
PsiI TTATAA 1 cut(s) 782
Psp124BI GAGCTC 2 cut(s) 470, 704
PspFI CCCAGC 1 cut(s) 559
PspN4I GGNNCC 3 cut(s) 6, 322, 606
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 2 cut(s) 410, 832
RsaNI GTAC 2 cut(s) 409, 831
RseI CAYNNNNRTG 3 cut(s) 222, 271, 578
SacI GAGCTC 2 cut(s) 470, 704
Sau3AI GATC 2 cut(s) 297, 426
Sau96I GGNCC 1 cut(s) 320
ScrFI CCNGG 4 cut(s) 318, 326, 327, 775
SduI GDGCHC 2 cut(s) 470, 704
SfaNI GCATC 5 cut(s) 9, 351, 459, 606, 649
SfcI CTRYAG 2 cut(s) 414, 631
SinI GGWCC 1 cut(s) 320
SmaI CCCGGG 1 cut(s) 327
SmiMI CAYNNNNRTG 3 cut(s) 222, 271, 578
Sse9I AATT 1 cut(s) 681
SseBI AGGCCT 1 cut(s) 489
SsiI CCGC 6 cut(s) 47, 204, 208, 419, 521, 549
SstI GAGCTC 2 cut(s) 470, 704
StuI AGGCCT 1 cut(s) 489
StyD4I CCNGG 4 cut(s) 316, 324, 325, 773
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 3 cut(s) 221, 434, 727
TaiI ACGT 1 cut(s) 150
TaqI TCGA 6 cut(s) 168, 258, 345, 436, 464, 624
TasI AATT 1 cut(s) 681
TauI GCSGC 6 cut(s) 49, 207, 210, 422, 524, 551
TfiI GAWTC 4 cut(s) 194, 248, 402, 626
TscAI CASTG 2 cut(s) 192, 224
TseI GCWGC 4 cut(s) 110, 413, 416, 746
TspDTI ATGAA 4 cut(s) 42, 365, 745, 832
TspGWI ACGGA 2 cut(s) 153, 546
TspMI CCCGGG 1 cut(s) 325
TspRI CASTG 2 cut(s) 192, 224
VpaK11BI GGWCC 1 cut(s) 320
XceI RCATGY 1 cut(s) 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XmaI CCCGGG 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.