Rroxscaffold_5G00386070

Short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015203
Physical Location & Seq
Reverse (-)
65025248 .. 65026202
955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386070.1

Sequence Viewer

Length: 873 bp
ATGGAGCCGAAAATTCTACCGCGTGAACTGGATGAAGAAGCACGCATACTGCCTTCGAAGAAGTTGGTGAACAAAGTCGCAGTTATAACTGGGGGTGCAAGAGGGATTGGAGCTGCTGCGGCCAAGCTATTTGCCCGAAACGGCGCCCATGTTGTGATTGCCGACATACTTGACGATTTGGGAGCTGCACTGGCTGACTCTATTGGGGGACGGTACATACACTGTGACGTGTCCAAGGAATCTGATGTTGAATCAGCTATTGAGCTTGCACTCGGAAGGACAGGCCAGCTTGACATCATGTTCAACAATGCTGGTATTTCTGGTCCGTCGGGGAGTATAACCAGCCTTGACATGGGAAAGGTGCAGCAACTGCTCTCAGTAAACACTTTCGGCTGTTTACATGGGATCAAGTATGCAGCAGCAGCTATGATCAAATCCAGAACAGGCGGAAGCATCATCTGCACATCGAGCACTGCAGCTATCATGGGAGGCCTTGGGGGACATGCCTACACATTGTCAAAGGAGTCCATCAATGGACTGGTGAGAAGCGCTGCATGTGAGTTGGGAGTCCATGGCATTCGTGTGAACAGTATTTGTCCACACGGGGTTCCTTCAGAGATGCTGGTCGCTGCCTACAGAGAGATTCTTGGGAAAGAGGATATGCAAGCTGAAGATGTAAGCAAGATTGTGGGAGAAAGGGCGAGCCTGTTGCGTGGCAGATGTGGAACCGTGGAAGATGTCGCAGAAGCTGCATTATTCCTTGCTAGTGAAGACTCAGGGTTCATAACAGCCCATAATCTTGTCCTTGATGGGGGTTATACTTCTGCTGTCAGTACCATGAGCTTCATTTATCAATTTACCAATAGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

290

Amino Acids

30.27

Weight (kDa)

5.82

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 25 - 209 9.9e-45 short chain dehydrogenase
KR PF08659 28 - 184 8.9e-07 KR domain
adh_short_C2 PF13561 32 - 274 1.2e-55 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015203)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42960
fragaria_vesca FvH4_4g35960
malus_domestica MD13G1013400.v1.1 MD16G1010300.v1.1
prunus_persica Prupe.1G340300_v2.0.a1
pyrus_communis pycom16g01010
rosa_laevigata RLG00000005727
rosa_multiflora Rmu_ssc0000107.1_g000001 Rmu_ssc0000253.1_g000009
rosa_roxburghii Rroxscaffold_5G00386070
rosa_rugosa Rorug04G0362800
rosa_samantha Rh4AG424200 Rh4BG436300 Rh4CG450900 Rh4DG432800
rosa_wichuraiana Rw4G036370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 143
AccII CGCG 1 cut(s) 22
AciI CCGC 3 cut(s) 20, 119, 447
AclWI GGATC 1 cut(s) 413
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 2 cut(s) 597, 690
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 2 cut(s) 215, 835
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 2 cut(s) 352, 811
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 2 cut(s) 251, 304
AjiI CACGTC 1 cut(s) 229
Alw21I GWGCWC 1 cut(s) 473
AlwI GGATC 1 cut(s) 413
AlwNI CAGNNNCTG 2 cut(s) 370, 749
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 3 cut(s) 120, 283, 490
ApoI RAATTY 1 cut(s) 12
AspLEI GCGC 2 cut(s) 146, 551
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 79, 553
AsuII TTCGAA 1 cut(s) 56
AvaII GGWCC 1 cut(s) 323
BanI GGYRCC 1 cut(s) 143
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BbsI GAAGAC 1 cut(s) 777
Bbv12I GWGCWC 1 cut(s) 473
BccI CCATC 2 cut(s) 536, 803
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 691, 725
BclI TGATCA 1 cut(s) 429
BfaI CTAG 1 cut(s) 765
BfmI CTRYAG 2 cut(s) 474, 634
BfoI RGCGCY 2 cut(s) 147, 552
Bme18I GGWCC 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 4 cut(s) 6, 145, 609, 727
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 462, 609
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 777
Bpu14I TTCGAA 1 cut(s) 56
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 4 cut(s) 234, 493, 571, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 811
Bse1I ACTGG 4 cut(s) 33, 94, 195, 543
BseDI CCNNGG 4 cut(s) 234, 493, 571, 729
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 2 cut(s) 352, 811
BseMII CTCAG 2 cut(s) 390, 789
BseNI ACTGG 4 cut(s) 33, 94, 195, 543
BsgI GTGCAG 3 cut(s) 171, 383, 445
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 3 cut(s) 122, 285, 492
BshNI GGYRCC 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 473
BslFI GGGAC 2 cut(s) 222, 513
BslI CCNNNNNNNGG 2 cut(s) 352, 811
BsmFI GGGAC 2 cut(s) 222, 513
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 3 cut(s) 122, 285, 492
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 2 cut(s) 405, 429
Bsp19I CCATGG 1 cut(s) 571
BspACI CCGC 3 cut(s) 20, 119, 447
BspANI GGCC 3 cut(s) 122, 285, 492
BspCNI CTCAG 2 cut(s) 389, 788
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 4 cut(s) 6, 145, 609, 727
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 1 cut(s) 413
BspT104I TTCGAA 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 143
BsrI ACTGG 4 cut(s) 33, 94, 195, 543
BssECI CCNNGG 4 cut(s) 234, 493, 571, 729
BssMI GATC 2 cut(s) 405, 429
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 3 cut(s) 234, 493, 571
Bst4CI ACNGT 4 cut(s) 213, 224, 590, 730
BstACI GRCGYC 1 cut(s) 144
BstAPI GCANNNNNTGC 3 cut(s) 370, 459, 749
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 5 cut(s) 43, 267, 287, 666, 703
BstDEI CTNAG 2 cut(s) 376, 775
BstDSI CCRYGG 2 cut(s) 571, 729
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 2 cut(s) 147, 552
BstHHI GCGC 2 cut(s) 146, 551
BstKTI GATC 2 cut(s) 408, 432
BstMBI GATC 2 cut(s) 405, 429
BstMWI GCNNNNNNNGC 7 cut(s) 119, 191, 370, 422, 459, 468, 749
BstNSI RCATGY 2 cut(s) 506, 558
BstSFI CTRYAG 2 cut(s) 474, 634
BstUI CGCG 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 777
BsuRI GGCC 3 cut(s) 122, 285, 492
BtgI CCRYGG 2 cut(s) 571, 729
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 37
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 188, 220, 471
Cac8I GCNNGC 5 cut(s) 43, 267, 287, 666, 703
CaiI CAGNNNCTG 2 cut(s) 370, 749
CfoI GCGC 2 cut(s) 146, 551
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 214, 834
CviAII CATG 9 cut(s) 149, 298, 352, 401, 484, 503, 555, 572, 838
CviQI GTAC 2 cut(s) 214, 834
DdeI CTNAG 2 cut(s) 376, 775
DinI GGCGCC 1 cut(s) 145
DpnI GATC 2 cut(s) 407, 431
DpnII GATC 2 cut(s) 405, 429
EaeI YGGCCR 1 cut(s) 120
EciI GGCGGA 1 cut(s) 462
Eco130I CCWWGG 3 cut(s) 234, 493, 571
Eco147I AGGCCT 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 323
Eco47III AGCGCT 1 cut(s) 550
Eco57I CTGAAG 2 cut(s) 597, 690
EcoT14I CCWWGG 3 cut(s) 234, 493, 571
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 3 cut(s) 234, 493, 571
FaeI CATG 9 cut(s) 152, 301, 355, 404, 487, 506, 558, 575, 841
FaqI GGGAC 2 cut(s) 222, 513
FatI CATG 9 cut(s) 148, 297, 351, 400, 483, 502, 554, 571, 837
FbaI TGATCA 1 cut(s) 429
FokI GGATG 1 cut(s) 44
FspBI CTAG 1 cut(s) 765
GlaI GCGC 2 cut(s) 145, 550
HaeII RGCGCY 2 cut(s) 147, 552
HaeIII GGCC 3 cut(s) 122, 285, 492
HhaI GCGC 2 cut(s) 146, 551
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 9 cut(s) 152, 301, 355, 404, 487, 506, 558, 575, 841
Hin6I GCGC 2 cut(s) 144, 549
HinP1I GCGC 2 cut(s) 144, 549
HinfI GANTC 7 cut(s) 197, 239, 251, 524, 567, 643, 773
HphI GGTGA 2 cut(s) 79, 553
Hpy166II GTNNAC 6 cut(s) 26, 70, 382, 398, 586, 599
Hpy188I TCNGA 3 cut(s) 244, 275, 616
Hpy188III TCNNGA 1 cut(s) 438
Hpy8I GTNNAC 6 cut(s) 26, 70, 382, 398, 586, 599
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 3 cut(s) 63, 270, 621
HpyCH4III ACNGT 4 cut(s) 213, 224, 590, 730
HpyCH4IV ACGT 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 7 cut(s) 119, 191, 370, 422, 459, 468, 749
HpyF3I CTNAG 2 cut(s) 376, 775
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 9 cut(s) 152, 301, 355, 404, 487, 506, 558, 575, 841
HspAI GCGC 2 cut(s) 144, 549
KasI GGCGCC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 2 cut(s) 405, 429
LmnI GCTCC 3 cut(s) 4, 110, 182
LweI GCATC 2 cut(s) 462, 609
MaeI CTAG 1 cut(s) 765
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 407, 431
MboI GATC 2 cut(s) 405, 429
MboII GAAGA 5 cut(s) 47, 70, 683, 746, 782
MhlI GDGCHC 1 cut(s) 473
MluCI AATT 2 cut(s) 12, 854
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 4 cut(s) 191, 533, 576, 767
MnlI CCTC 3 cut(s) 95, 482, 649
MslI CAYNNNNRTG 1 cut(s) 581
Mva1269I GAATGC 1 cut(s) 576
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 7 cut(s) 119, 191, 370, 422, 459, 468, 749
NarI GGCGCC 1 cut(s) 144
NcoI CCATGG 1 cut(s) 571
NdeII GATC 2 cut(s) 405, 429
NlaIII CATG 9 cut(s) 152, 301, 355, 404, 487, 506, 558, 575, 841
NlaIV GGNNCC 4 cut(s) 6, 145, 609, 727
NmuCI GTSAC 1 cut(s) 224
NspI RCATGY 2 cut(s) 506, 558
NspV TTCGAA 1 cut(s) 56
PceI AGGCCT 1 cut(s) 492
PctI GAATGC 1 cut(s) 576
PfeI GAWTC 3 cut(s) 239, 251, 643
PleI GAGTC 4 cut(s) 191, 532, 575, 767
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 4 cut(s) 191, 532, 575, 767
PsiI TTATAA 1 cut(s) 86
PspN4I GGNNCC 4 cut(s) 6, 145, 609, 727
PspPI GGNCC 1 cut(s) 323
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 2 cut(s) 370, 749
RsaI GTAC 2 cut(s) 215, 835
RsaNI GTAC 2 cut(s) 214, 834
RseI CAYNNNNRTG 1 cut(s) 581
Sau3AI GATC 2 cut(s) 405, 429
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 4 cut(s) 191, 533, 576, 767
SduI GDGCHC 1 cut(s) 473
SfaNI GCATC 2 cut(s) 462, 609
SfcI CTRYAG 2 cut(s) 474, 634
SfoI GGCGCC 1 cut(s) 145
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 581
Sse9I AATT 2 cut(s) 12, 854
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 3 cut(s) 20, 119, 447
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 1 cut(s) 765
StuI AGGCCT 1 cut(s) 492
StyI CCWWGG 3 cut(s) 234, 493, 571
TaaI ACNGT 4 cut(s) 213, 224, 590, 730
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 56, 467
TasI AATT 2 cut(s) 12, 854
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 3 cut(s) 239, 251, 643
TscAI CASTG 3 cut(s) 195, 227, 478
TseFI GTSAC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 3 cut(s) 48, 772, 835
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 3 cut(s) 195, 227, 478
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 12
XceI RCATGY 2 cut(s) 506, 558
XcmI CCANNNNNNNNNTGG 2 cut(s) 349, 535
XspI CTAG 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.