Rh4AG424200

Short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015203
Physical Location & Seq
Reverse (-)
72079524 .. 72080483
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG424200.1

Sequence Viewer

Length: 876 bp
ATGGAGCCGAAAATTCTACTGCGTGAACCGGATGAAGAAGCACGCATACTGCCTTCGAAGAAGAAGTTGGTGAACAAAGTCGCAGTTATAACTGGGGGTGCAAGAGGGATTGGAGCTGCTGCGGCCAAGCTATTTGCCCGAAACGGCGCCCACGTTGTGATTGCCGACATACTTGACGATTTGGGAGCTGCACTGGCCGACTCTATTGGGGGACGGTACATACACTGTGATGTGTCCAAGGAATCTGATGTTGAATCAGCTATTGAGCTTGCACTCGGAAGGACAGGCCAGCTTGACATCATGTTCAACAATGCTGGTATTTCTGGCCCGTCGGGGAGTATAACCAGCCTTGACATGGGAAAGGTGCAGCAACTGCTCTCGGTAAACACTTTTGGCTGTTTACATGGGATTAAGTATGCAGCAGCAGCTATGATCAAAACCAGAACAGGTGGGAGCATCATCTGCACATCGAGCACTGCAGCTAGCATGGGAGGCCTTGGTGGACATGCCTACACATTGTCAAAGGAATCCATCAATGGACTGGTGAGAAGCGCCGCATGTGAGTTGGGAGTCCATGGCATTCGTGTGAACAGTATTTCTCCACACGGGGTTCCTTCAGAGATGCTGGTCGCTGCCTACAAAGAGATTCTTGGGAAAGAGGATATGCAGGCCGAAGATGTAAGCAAGATTGTGGCAGAAAGGGCGAGCTTGTTGCGTGGCAGATGTGGAACCGTGGAAGATGTCGCAGAAGCTGCATTATTCCTTGCCAGTGAAGACTCAGGGTTCATAACAGCCCATAATCTTGTCCTTGATGGGGGTTATACTTCTGCTGTCAGTACCATGAGCTTCATTTATCAATTTACCAATAGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

291

Amino Acids

30.35

Weight (kDa)

5.99

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 26 - 211 6.9e-44 short chain dehydrogenase
KR PF08659 29 - 185 3.7e-06 KR domain
adh_short_C2 PF13561 33 - 275 6.9e-56 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015203)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42960
fragaria_vesca FvH4_4g35960
malus_domestica MD13G1013400.v1.1 MD16G1010300.v1.1
prunus_persica Prupe.1G340300_v2.0.a1
pyrus_communis pycom16g01010
rosa_laevigata RLG00000005727
rosa_multiflora Rmu_ssc0000107.1_g000001 Rmu_ssc0000253.1_g000009
rosa_roxburghii Rroxscaffold_5G00386070
rosa_rugosa Rorug04G0362800
rosa_samantha Rh4AG424200 Rh4BG436300 Rh4CG450900 Rh4DG432800
rosa_wichuraiana Rw4G036370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 146
AciI CCGC 2 cut(s) 122, 555
AcoI YGGCCR 2 cut(s) 123, 195
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 2 cut(s) 218, 838
AfiI CCNNNNNNNGG 2 cut(s) 355, 814
AgsI TTSAA 2 cut(s) 254, 307
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
Alw21I GWGCWC 1 cut(s) 476
AlwNI CAGNNNCTG 2 cut(s) 373, 752
AoxI GGCC 6 cut(s) 123, 195, 286, 325, 493, 669
ApoI RAATTY 1 cut(s) 12
AspLEI GCGC 2 cut(s) 149, 554
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 82, 556
AsuII TTCGAA 1 cut(s) 56
AsuNHI GCTAGC 1 cut(s) 482
BanI GGYRCC 1 cut(s) 146
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 1 cut(s) 476
BccI CCATC 2 cut(s) 539, 806
BceAI ACGGC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 694, 728
BclI TGATCA 1 cut(s) 432
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 2 cut(s) 150, 555
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 4 cut(s) 6, 148, 612, 730
BmrI ACTGGG 1 cut(s) 102
BmsI GCATC 2 cut(s) 465, 612
BmtI GCTAGC 1 cut(s) 486
BmuI ACTGGG 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 780
Bpu14I TTCGAA 1 cut(s) 56
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 4 cut(s) 237, 496, 574, 732
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 814
Bse1I ACTGG 4 cut(s) 97, 198, 546, 768
BseDI CCNNGG 4 cut(s) 237, 496, 574, 732
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 2 cut(s) 355, 814
BseMII CTCAG 1 cut(s) 792
BseNI ACTGG 4 cut(s) 97, 198, 546, 768
BsgI GTGCAG 3 cut(s) 174, 386, 448
BshFI GGCC 6 cut(s) 125, 197, 288, 327, 495, 671
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 476
BsiSI CCGG 1 cut(s) 29
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 355, 814
BsmFI GGGAC 1 cut(s) 225
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 6 cut(s) 125, 197, 288, 327, 495, 671
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 1 cut(s) 432
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 2 cut(s) 122, 555
BspANI GGCC 6 cut(s) 125, 197, 288, 327, 495, 671
BspCNI CTCAG 1 cut(s) 791
BspLI GGNNCC 4 cut(s) 6, 148, 612, 730
BspMAI CTGCAG 1 cut(s) 481
BspOI GCTAGC 1 cut(s) 486
BspT104I TTCGAA 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 146
BsrI ACTGG 4 cut(s) 97, 198, 546, 768
BssECI CCNNGG 4 cut(s) 237, 496, 574, 732
BssMI GATC 1 cut(s) 432
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 3 cut(s) 237, 496, 574
Bst4CI ACNGT 4 cut(s) 216, 227, 593, 733
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 3 cut(s) 373, 462, 752
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 6 cut(s) 43, 270, 290, 484, 669, 706
BstDEI CTNAG 1 cut(s) 778
BstDSI CCRYGG 2 cut(s) 574, 732
BstF5I GGATG 1 cut(s) 37
BstH2I RGCGCY 2 cut(s) 150, 555
BstHHI GCGC 2 cut(s) 149, 554
BstKTI GATC 1 cut(s) 435
BstMBI GATC 1 cut(s) 432
BstMWI GCNNNNNNNGC 9 cut(s) 122, 194, 373, 425, 462, 471, 492, 701, 752
BstNSI RCATGY 2 cut(s) 509, 561
BstSFI CTRYAG 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 780
BsuRI GGCC 6 cut(s) 125, 197, 288, 327, 495, 671
BtgI CCRYGG 2 cut(s) 574, 732
BtsCI GGATG 1 cut(s) 37
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 4 cut(s) 191, 223, 474, 775
Cac8I GCNNGC 6 cut(s) 43, 270, 290, 484, 669, 706
CaiI CAGNNNCTG 2 cut(s) 373, 752
CfoI GCGC 2 cut(s) 149, 554
Cfr13I GGNCC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 217, 837
CviAII CATG 8 cut(s) 301, 355, 404, 487, 506, 558, 575, 841
CviQI GTAC 2 cut(s) 217, 837
DdeI CTNAG 1 cut(s) 778
DinI GGCGCC 1 cut(s) 148
DpnI GATC 1 cut(s) 434
DpnII GATC 1 cut(s) 432
DraIII CACNNNGTG 1 cut(s) 157
EaeI YGGCCR 2 cut(s) 123, 195
Eco130I CCWWGG 3 cut(s) 237, 496, 574
Eco147I AGGCCT 1 cut(s) 495
Eco57I CTGAAG 1 cut(s) 600
EcoT14I CCWWGG 3 cut(s) 237, 496, 574
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 3 cut(s) 237, 496, 574
FaeI CATG 8 cut(s) 304, 358, 407, 490, 509, 561, 578, 844
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FaqI GGGAC 1 cut(s) 225
FatI CATG 8 cut(s) 300, 354, 403, 486, 505, 557, 574, 840
FbaI TGATCA 1 cut(s) 432
FokI GGATG 1 cut(s) 44
FspBI CTAG 1 cut(s) 483
GlaI GCGC 2 cut(s) 148, 553
HaeII RGCGCY 2 cut(s) 150, 555
HaeIII GGCC 6 cut(s) 125, 197, 288, 327, 495, 671
HapII CCGG 1 cut(s) 29
HhaI GCGC 2 cut(s) 149, 554
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 8 cut(s) 304, 358, 407, 490, 509, 561, 578, 844
Hin6I GCGC 2 cut(s) 147, 552
HinP1I GCGC 2 cut(s) 147, 552
HinfI GANTC 7 cut(s) 200, 242, 254, 527, 570, 646, 776
HpaII CCGG 1 cut(s) 29
HphI GGTGA 2 cut(s) 82, 556
Hpy166II GTNNAC 6 cut(s) 26, 73, 385, 401, 503, 589
Hpy188I TCNGA 3 cut(s) 247, 278, 619
Hpy8I GTNNAC 6 cut(s) 26, 73, 385, 401, 503, 589
Hpy99I CGWCG 1 cut(s) 334
HpyAV CCTTC 3 cut(s) 63, 273, 624
HpyCH4III ACNGT 4 cut(s) 216, 227, 593, 733
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 9 cut(s) 101, 191, 272, 367, 419, 465, 479, 667, 755
HpyF10VI GCNNNNNNNGC 9 cut(s) 122, 194, 373, 425, 462, 471, 492, 701, 752
HpyF3I CTNAG 1 cut(s) 778
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 8 cut(s) 304, 358, 407, 490, 509, 561, 578, 844
HspAI GCGC 2 cut(s) 147, 552
KasI GGCGCC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 1 cut(s) 432
LmnI GCTCC 4 cut(s) 4, 113, 185, 453
LweI GCATC 2 cut(s) 465, 612
MaeI CTAG 1 cut(s) 483
MaeII ACGT 1 cut(s) 153
MalI GATC 1 cut(s) 434
MboI GATC 1 cut(s) 432
MboII GAAGA 6 cut(s) 47, 70, 73, 686, 749, 785
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 2 cut(s) 12, 857
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 194, 579, 770
MnlI CCTC 3 cut(s) 98, 485, 652
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 2 cut(s) 228, 584
MspI CCGG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 579
MwoI GCNNNNNNNGC 9 cut(s) 122, 194, 373, 425, 462, 471, 492, 701, 752
NarI GGCGCC 1 cut(s) 147
NcoI CCATGG 1 cut(s) 574
NdeII GATC 1 cut(s) 432
NheI GCTAGC 1 cut(s) 482
NlaIII CATG 8 cut(s) 304, 358, 407, 490, 509, 561, 578, 844
NlaIV GGNNCC 4 cut(s) 6, 148, 612, 730
NspI RCATGY 2 cut(s) 509, 561
NspV TTCGAA 1 cut(s) 56
PceI AGGCCT 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 1 cut(s) 579
PfeI GAWTC 4 cut(s) 242, 254, 527, 646
PleI GAGTC 3 cut(s) 194, 578, 770
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 3 cut(s) 194, 578, 770
PsiI TTATAA 1 cut(s) 89
PspN4I GGNNCC 4 cut(s) 6, 148, 612, 730
PspPI GGNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 2 cut(s) 373, 752
RsaI GTAC 2 cut(s) 218, 838
RsaNI GTAC 2 cut(s) 217, 837
RseI CAYNNNNRTG 2 cut(s) 228, 584
SaqAI TTAA 1 cut(s) 411
Sau3AI GATC 1 cut(s) 432
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 3 cut(s) 194, 579, 770
SduI GDGCHC 1 cut(s) 476
SfaNI GCATC 2 cut(s) 465, 612
SfcI CTRYAG 1 cut(s) 477
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 56
SmiMI CAYNNNNRTG 2 cut(s) 228, 584
Sse9I AATT 2 cut(s) 12, 857
SseBI AGGCCT 1 cut(s) 495
SsiI CCGC 2 cut(s) 122, 555
SspDI GGCGCC 1 cut(s) 146
SspMI CTAG 1 cut(s) 483
StuI AGGCCT 1 cut(s) 495
StyI CCWWGG 3 cut(s) 237, 496, 574
TaaI ACNGT 4 cut(s) 216, 227, 593, 733
TaiI ACGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 56, 470
TasI AATT 2 cut(s) 12, 857
TauI GCSGC 2 cut(s) 125, 557
TfiI GAWTC 4 cut(s) 242, 254, 527, 646
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 4 cut(s) 198, 230, 481, 775
TspDTI ATGAA 3 cut(s) 48, 775, 838
TspRI CASTG 4 cut(s) 198, 230, 481, 775
XapI RAATTY 1 cut(s) 12
XceI RCATGY 2 cut(s) 509, 561
XcmI CCANNNNNNNNNTGG 2 cut(s) 352, 538
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.