MD13G1018800.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012430
Physical Location & Seq
Reverse (-)
1178594 .. 1181242
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1018800.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGTGAAATCGGCGGAGAAGGGCGGAGGAGATGGGTCGGGGCCGGCGGGGTCGGTGGCTCCTTTTCTCAGAAAATGCTACGAGATGGTGGATGATAAGGACACCGACTCCATAATCTCGTGGAGTGAAACCGGCGACAGCTTTGCTATATTGGACATGGCCCAGTTCTCGATTTCAATGCTGCCCAAGTACTTCAAGCACAGTAACTTTTCTAGCTTCATGAGACAGCTCAATATCTATGGCTTTAGAAAAATAGATTCAGATCGTTGGGTGTTTGCAAACGAAGGATTTATTCGAGGTCAGAAAATTTTGTTGAAGAATATTGCTAGGAGGAAACATCCACAGGGCACAGATCAGAAAAAAGCATCACAGCAAAGGCCGGATAGGCCATCTGAAAACATTAGTGAAAATGGTCTATGGAGGGAAGTTGAGAACCTGAAGACTGATAAAGTTGCTATGAAGCAAGAATTGGCAAAGCTTAGGCAGCACCAGGAAACTTCGCAAAATAACTTGCGCCTCTTGAGGAACCGCCTTTGTGGAATGGAGAAGAATCAGCAGCAGATGCTGTCGTTTCTAGTGACGGCCATGCAAAGTCCTGGTTTTCTGGTTCAGCTTCTTCATCCAAAAGAAAATAATTGGCGAATTGCTGAAGCTGGCGATGTGCTAGAACAGTGTGGAGATGATGATAGACCAGTGGTTTCTAGTGGTGCAATAGTGAGGTACCAACCACCGATGATTGATGCCCCAAAGCATGTACTCCCACCAAATTCAGGCTCAGAGAAACAACCGGAATCTGATGCTTATCTAGATGGAATGGAAGATTTTGTTGTGAATGCTGATTTCATGAAAATGCTAATGGATGAAAAGTCGAGCCCTGTAGAAAATCACGACCCATATACCCTACCAGATATATCTGACGATGGTGCATGGGAGCAGCTTCTTTTAGGTGGTCCTTTCTTGGAAAATATTGAAGGCACAATGGAAAATGGTGAAGAGCATGCTGACACTAGAATGGAGGTGGAATCAATAGTATCTGATCTGGACGATTCACACAATTTTGATTATTTGGTCGAGCAAATGAAGAAATCTCAGAACTTTGCATTAGAATCAGCAGTTTATGGATCTAATACGGAGAGCTCTCAAAACTTGGAATTTATAACTGAACACATGGGACATTTGGCTTCTGACTCTAACAACATGGAACAAAATCAGGAACGTAGCAGGATAACATCTGTGTAA

Protein Analysis

413

Amino Acids

46.61

Weight (kDa)

4.91

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 22 - 111 5.4e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67970
fragaria_vesca FvH4_4g33360 FvH4_4g33360
malus_domestica MD13G1018800.v1.1 MD16G1016700.v1.1
prunus_persica Prupe.1G335100_v2.0.a1
pyrus_communis pycom13g01590
rosa_chinensis RchiOBHm_Chr4g0442621
rosa_laevigata RLG00000005998
rosa_multiflora Rmu_co8503207.1_g000002 Rmu_sc0025219.1_g000001 Rmu_ssc0000204.1_g000036
rosa_roxburghii Rroxscaffold_5G00383420
rosa_rugosa Rorug04G0341400
rosa_samantha Rh4AG394100 Rh4BG407100 Rh4CG422500 Rh4DG401100
rosa_wichuraiana Rw4G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
Acc65I GGTACC 1 cut(s) 720
AccB1I GGYRCC 1 cut(s) 720
AciI CCGC 4 cut(s) 14, 24, 47, 529
AclWI GGATC 1 cut(s) 1129
AcoI YGGCCR 1 cut(s) 582
AcsI RAATTY 3 cut(s) 306, 766, 1151
AcuI CTGAAG 2 cut(s) 458, 669
AfaI GTAC 3 cut(s) 191, 722, 756
AfiI CCNNNNNNNGG 1 cut(s) 770
AgsI TTSAA 4 cut(s) 177, 196, 316, 971
AjnI CCWGG 2 cut(s) 489, 595
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AluBI AGCT 8 cut(s) 141, 216, 229, 478, 613, 653, 937, 1137
AluI AGCT 8 cut(s) 141, 216, 229, 478, 613, 653, 937, 1137
Alw21I GWGCWC 1 cut(s) 1139
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 1 cut(s) 1129
AlwNI CAGNNNCTG 1 cut(s) 565
AoxI GGCC 5 cut(s) 41, 159, 377, 386, 582
ApeKI GCWGC 4 cut(s) 181, 484, 556, 934
ApoI RAATTY 3 cut(s) 306, 766, 1151
Asp718I GGTACC 1 cut(s) 720
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 3 cut(s) 41, 160, 950
AsuHPI GGTGA 2 cut(s) 16, 1001
AvaII GGWCC 1 cut(s) 950
BaeGI GKGCMC 1 cut(s) 350
BanI GGYRCC 1 cut(s) 720
BanII GRGCYC 2 cut(s) 875, 1139
BauI CACGAG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 1139
BbvI GCAGC 4 cut(s) 168, 496, 568, 946
BccI CCATC 5 cut(s) 26, 79, 397, 803, 914
BceAI ACGGC 1 cut(s) 597
BcgI CGANNNNNNTGC 4 cut(s) 125, 159, 160, 194
BciT130I CCWGG 2 cut(s) 491, 597
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 7 cut(s) 213, 327, 575, 665, 702, 806, 1008
BfmI CTRYAG 1 cut(s) 876
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 4 cut(s) 182, 485, 557, 935
BlsI GCNGC 4 cut(s) 183, 486, 558, 936
BmcAI AGTACT 1 cut(s) 191
Bme1390I CCNGG 2 cut(s) 491, 597
Bme18I GGWCC 1 cut(s) 950
BmgT120I GGNCC 3 cut(s) 41, 160, 950
BmiI GGNNCC 4 cut(s) 42, 60, 527, 722
BmrFI CCNGG 2 cut(s) 491, 597
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 4 cut(s) 374, 552, 730, 787
BmuI ACTGGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 446
Bpu10I CCTNAGC 1 cut(s) 479
BpuEI CTTGAG 1 cut(s) 541
BsaBI GATNNNNATC 2 cut(s) 261, 801
BsaWI WCCGGW 1 cut(s) 787
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse118I RCCGGY 2 cut(s) 43, 131
Bse1I ACTGG 2 cut(s) 163, 692
Bse8I GATNNNNATC 2 cut(s) 261, 801
BseBI CCWGG 2 cut(s) 491, 597
BseGI GGATG 4 cut(s) 97, 337, 619, 865
BseJI GATNNNNATC 2 cut(s) 261, 801
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMII CTCAG 3 cut(s) 82, 789, 1103
BseNI ACTGG 2 cut(s) 163, 692
BseRI GAGGAG 1 cut(s) 42
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 4 cut(s) 168, 496, 568, 946
BshFI GGCC 5 cut(s) 43, 161, 379, 388, 584
BshNI GGYRCC 1 cut(s) 720
BsiHKAI GWGCWC 1 cut(s) 1139
BsiSI CCGG 4 cut(s) 44, 132, 380, 788
BslFI GGGAC 1 cut(s) 1185
BslI CCNNNNNNNGG 1 cut(s) 770
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 1185
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 5 cut(s) 43, 161, 379, 388, 584
Bsp1286I GDGCHC 3 cut(s) 350, 875, 1139
Bsp143I GATC 4 cut(s) 262, 352, 1036, 1121
BspACI CCGC 4 cut(s) 14, 24, 47, 529
BspANI GGCC 5 cut(s) 43, 161, 379, 388, 584
BspCNI CTCAG 3 cut(s) 81, 788, 1102
BspHI TCATGA 2 cut(s) 219, 843
BspLI GGNNCC 4 cut(s) 42, 60, 527, 722
BspPI GGATC 1 cut(s) 1129
BspQI GCTCTTC 1 cut(s) 987
BspT107I GGYRCC 1 cut(s) 720
BsrFI RCCGGY 2 cut(s) 43, 131
BsrI ACTGG 2 cut(s) 163, 692
BssAI RCCGGY 2 cut(s) 43, 131
BssMI GATC 4 cut(s) 262, 352, 1036, 1121
BssSI CACGAG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 491, 597
Bst4CI ACNGT 2 cut(s) 203, 672
Bst6I CTCTTC 1 cut(s) 987
BstAPI GCANNNNNTGC 1 cut(s) 562
BstC8I GCNNGC 3 cut(s) 45, 655, 999
BstDEI CTNAG 4 cut(s) 68, 479, 775, 1089
BstF5I GGATG 4 cut(s) 97, 337, 619, 865
BstHHI GCGC 1 cut(s) 516
BstKTI GATC 4 cut(s) 265, 355, 1039, 1124
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 4 cut(s) 262, 352, 1036, 1121
BstMWI GCNNNNNNNGC 3 cut(s) 385, 484, 562
BstNI CCWGG 2 cut(s) 491, 597
BstNSI RCATGY 2 cut(s) 755, 1001
BstSCI CCNGG 2 cut(s) 489, 595
BstSFI CTRYAG 1 cut(s) 876
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 4 cut(s) 168, 496, 568, 946
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 1121
BsuRI GGCC 5 cut(s) 43, 161, 379, 388, 584
BtgZI GCGATG 1 cut(s) 672
BtsCI GGATG 4 cut(s) 97, 337, 619, 865
BtsIMutI CAGTG 2 cut(s) 677, 699
Cac8I GCNNGC 3 cut(s) 45, 655, 999
CaiI CAGNNNCTG 1 cut(s) 565
CciI TCATGA 2 cut(s) 219, 843
CfoI GCGC 1 cut(s) 516
Cfr10I RCCGGY 2 cut(s) 43, 131
Cfr13I GGNCC 3 cut(s) 41, 160, 950
Csp6I GTAC 3 cut(s) 190, 721, 755
CviAII CATG 9 cut(s) 157, 220, 586, 752, 844, 927, 998, 1168, 1198
CviQI GTAC 3 cut(s) 190, 721, 755
DdeI CTNAG 4 cut(s) 68, 479, 775, 1089
DpnI GATC 4 cut(s) 264, 354, 1038, 1123
DpnII GATC 4 cut(s) 262, 352, 1036, 1121
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 1 cut(s) 987
EarI CTCTTC 1 cut(s) 987
EciI GGCGGA 2 cut(s) 29, 39
Ecl136II GAGCTC 1 cut(s) 1137
Eco24I GRGCYC 2 cut(s) 875, 1139
Eco47I GGWCC 1 cut(s) 950
Eco53kI GAGCTC 1 cut(s) 1137
Eco57I CTGAAG 2 cut(s) 458, 669
EcoICRI GAGCTC 1 cut(s) 1137
EcoRII CCWGG 2 cut(s) 489, 595
EcoT38I GRGCYC 2 cut(s) 875, 1139
FaeI CATG 9 cut(s) 160, 223, 589, 755, 847, 930, 1001, 1171, 1201
FaqI GGGAC 1 cut(s) 1185
FatI CATG 9 cut(s) 156, 219, 585, 751, 843, 926, 997, 1167, 1197
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 4 cut(s) 182, 485, 557, 935
FokI GGATG 4 cut(s) 104, 324, 606, 872
FriOI GRGCYC 2 cut(s) 875, 1139
Fsp4HI GCNGC 4 cut(s) 182, 485, 557, 935
FspBI CTAG 7 cut(s) 213, 327, 575, 665, 702, 806, 1008
GlaI GCGC 1 cut(s) 515
GluI GCNGC 4 cut(s) 182, 485, 557, 935
HaeIII GGCC 5 cut(s) 43, 161, 379, 388, 584
HapII CCGG 4 cut(s) 44, 132, 380, 788
HhaI GCGC 1 cut(s) 516
Hin1II CATG 9 cut(s) 160, 223, 589, 755, 847, 930, 1001, 1171, 1201
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 8 cut(s) 107, 257, 550, 791, 1022, 1046, 1106, 1187
HpaII CCGG 4 cut(s) 44, 132, 380, 788
HphI GGTGA 2 cut(s) 16, 1001
Hpy188III TCNNGA 8 cut(s) 169, 220, 520, 806, 844, 887, 1040, 1211
HpyAV CCTTC 3 cut(s) 13, 278, 965
HpyCH4III ACNGT 2 cut(s) 203, 672
HpyCH4IV ACGT 1 cut(s) 1216
HpyCH4V TGCA 5 cut(s) 278, 589, 710, 926, 1100
HpyF10VI GCNNNNNNNGC 3 cut(s) 385, 484, 562
HpyF3I CTNAG 4 cut(s) 68, 479, 775, 1089
HpySE526I ACGT 1 cut(s) 1216
Hsp92II CATG 9 cut(s) 160, 223, 589, 755, 847, 930, 1001, 1171, 1201
HspAI GCGC 1 cut(s) 514
KpnI GGTACC 1 cut(s) 724
KroI GCCGGC 1 cut(s) 43
KroNI GCCGGC 1 cut(s) 45
Kzo9I GATC 4 cut(s) 262, 352, 1036, 1121
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 2 cut(s) 64, 931
Lsp1109I GCAGC 4 cut(s) 168, 496, 568, 946
LweI GCATC 4 cut(s) 374, 552, 730, 787
MaeI CTAG 7 cut(s) 213, 327, 575, 665, 702, 806, 1008
MaeII ACGT 1 cut(s) 1216
MaeIII GTNAC 2 cut(s) 203, 577
MalI GATC 4 cut(s) 264, 354, 1038, 1123
MboI GATC 4 cut(s) 262, 352, 1036, 1121
MboII GAAGA 7 cut(s) 328, 451, 559, 608, 830, 1004, 1093
MflI RGATCY 1 cut(s) 1121
MhlI GDGCHC 3 cut(s) 350, 875, 1139
MluCI AATT 7 cut(s) 306, 467, 634, 642, 766, 1054, 1151
MlyI GAGTC 2 cut(s) 101, 1181
MnlI CCTC 8 cut(s) 20, 290, 324, 414, 516, 527, 711, 1009
MroNI GCCGGC 1 cut(s) 43
MslI CAYNNNNRTG 2 cut(s) 848, 1010
MspI CCGG 4 cut(s) 44, 132, 380, 788
MspR9I CCNGG 2 cut(s) 491, 597
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 2 cut(s) 491, 597
MwoI GCNNNNNNNGC 3 cut(s) 385, 484, 562
NaeI GCCGGC 1 cut(s) 45
NdeII GATC 4 cut(s) 262, 352, 1036, 1121
NgoMIV GCCGGC 1 cut(s) 43
NlaIII CATG 9 cut(s) 160, 223, 589, 755, 847, 930, 1001, 1171, 1201
NlaIV GGNNCC 4 cut(s) 42, 60, 527, 722
NmuCI GTSAC 1 cut(s) 577
NspI RCATGY 2 cut(s) 755, 1001
PaeI GCATGC 1 cut(s) 1001
PagI TCATGA 2 cut(s) 219, 843
PciSI GCTCTTC 1 cut(s) 987
PctI GAATGC 1 cut(s) 838
PdiI GCCGGC 1 cut(s) 45
PfeI GAWTC 6 cut(s) 257, 550, 791, 1022, 1046, 1106
PkrI GCNGC 4 cut(s) 183, 486, 558, 936
PleI GAGTC 2 cut(s) 101, 1181
PpsI GAGTC 2 cut(s) 101, 1181
PsiI TTATAA 1 cut(s) 1157
Psp124BI GAGCTC 1 cut(s) 1139
Psp6I CCWGG 2 cut(s) 489, 595
PspGI CCWGG 2 cut(s) 489, 595
PspN4I GGNNCC 4 cut(s) 42, 60, 527, 722
PspPI GGNCC 3 cut(s) 41, 160, 950
PstNI CAGNNNCTG 1 cut(s) 565
PsuI RGATCY 1 cut(s) 1121
RsaI GTAC 3 cut(s) 191, 722, 756
RsaNI GTAC 3 cut(s) 190, 721, 755
RseI CAYNNNNRTG 2 cut(s) 848, 1010
SacI GAGCTC 1 cut(s) 1139
SapI GCTCTTC 1 cut(s) 987
SatI GCNGC 4 cut(s) 182, 485, 557, 935
Sau3AI GATC 4 cut(s) 262, 352, 1036, 1121
Sau96I GGNCC 3 cut(s) 41, 160, 950
ScaI AGTACT 1 cut(s) 191
SchI GAGTC 2 cut(s) 101, 1181
ScrFI CCNGG 2 cut(s) 491, 597
SduI GDGCHC 3 cut(s) 350, 875, 1139
SfaNI GCATC 4 cut(s) 374, 552, 730, 787
SfcI CTRYAG 1 cut(s) 876
SfiI GGCCNNNNNGGCC 1 cut(s) 385
SinI GGWCC 1 cut(s) 950
SmiMI CAYNNNNRTG 2 cut(s) 848, 1010
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
SphI GCATGC 1 cut(s) 1001
Sse9I AATT 7 cut(s) 306, 467, 634, 642, 766, 1054, 1151
SsiI CCGC 4 cut(s) 14, 24, 47, 529
SspI AATATT 2 cut(s) 322, 967
SspMI CTAG 7 cut(s) 213, 327, 575, 665, 702, 806, 1008
SstI GAGCTC 1 cut(s) 1139
StyD4I CCNGG 2 cut(s) 489, 595
TaaI ACNGT 2 cut(s) 203, 672
TaiI ACGT 1 cut(s) 1219
TaqI TCGA 4 cut(s) 170, 295, 869, 1071
TasI AATT 7 cut(s) 306, 467, 634, 642, 766, 1054, 1151
TatI WGTACW 2 cut(s) 189, 754
TfiI GAWTC 6 cut(s) 257, 550, 791, 1022, 1046, 1106
TscAI CASTG 2 cut(s) 677, 699
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 4 cut(s) 181, 484, 556, 934
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 7 cut(s) 208, 473, 608, 832, 860, 876, 1094
TspGWI ACGGA 1 cut(s) 1145
TspRI CASTG 2 cut(s) 677, 699
VpaK11BI GGWCC 1 cut(s) 950
XapI RAATTY 3 cut(s) 306, 766, 1151
XbaI TCTAGA 1 cut(s) 805
XceI RCATGY 2 cut(s) 755, 1001
XspI CTAG 7 cut(s) 213, 327, 575, 665, 702, 806, 1008
ZrmI AGTACT 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.