pycom13g01590

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012430
Physical Location & Seq
Reverse (-)
1042965 .. 1044981
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g01590.2

Sequence Viewer

Length: 1209 bp
ATGGTGAAATCGGCGGAGAAGGGCGGAGGAGAAGGGTCGGGGCCGGTGGCTCCTTTTCTCAGAAAATGCTACGAGATGGTGGATGATAAGGACACCGACTCCATAATCACGTGGAGTGAAACCGGCGACAGCTTTGCTATATTGGACATGGCCCAGTTCTCGATTTCAATGCTGCCCAAGTACTTCAAGCACAGTAACTTTTCTAGCTTCATGAGACAGCTCAATATCTATGGCTTTAGAAAAATAGATTCAGATCGTTGGGTGTTTGCAAACGAAGGATTTATTCGAGGTCAGAAAATTTTGTTGAAGAATATTGCTAGGAGGAAACATCCACAGGGCACAGATCAGAAAAAAGCATCACAGCAAAGTCCGGATAGGCCATCTGAAAACATTAGTGAAAATGGTCTATGGAGGGAAATTGAGAACCTGAAGACTGATAAAGTTGCTCTGAAGCAAGAATTGGCAAAGCTTAGGCAGCACCAGGAAACTTCGCAAAATAACTTGCGCCTCTTGAGGGACCGCCTTTGTGGAATGGAGAAGAATCAGCAGCAGATGCTGTCGTTTCTAGTGATGGCCATGCAAAGTCCTGGGTTTCTGGTTCAGCTTCTTCAGCCAAAAGAAAATAATTGGCGAATTGCTGAAGCTGGTGATATGATAGAACAGTGTGTAGATGATGATAAACCAGTGGTTTCTAACGGTGCGATAGTGAGGTACCAACCACCGATGATTGAAGCCCCAAAGCATGTACTCCTACCGAATTCAGGCTCAGAGAAACAACCGGAATCTGATGCTTATCTAGATGGAATGGAAGATTCTGTTGTGAATGCTGATTTCATGAAAATGCTAATGGATGAAAGGTCAAGCCCTATAGAAAATCACGACCCATATACCCTACCGGATATATCTGACAATGGTGCATGGGAGCAGCTTCTTTTAGCTAGTCCTTTCTTGGAAAATATTGAAGGCACAAAGGAAAATGGTGAAGAGCATGCTGAGACTAGAATGGAGGTGGAACCAATAGTATCTGATCTGGACAATTCACGCGATTTTGATTATTTGGTCGAGCAAATGAAGAAATCTCAGAACTTTGCATTGGAATCAACAGTTTATGGAGCTAATATGGAGAGCTCTCAAAACTTGGAATTTATAACTGAACACATGGGACATTTGGCCTCTGACTCTAACATGGAAACGAAATCAGGAACGTAG

Protein Analysis

403

Amino Acids

45.65

Weight (kDa)

4.88

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67970
fragaria_vesca FvH4_4g33360 FvH4_4g33360
malus_domestica MD13G1018800.v1.1 MD16G1016700.v1.1
prunus_persica Prupe.1G335100_v2.0.a1
pyrus_communis pycom13g01590
rosa_chinensis RchiOBHm_Chr4g0442621
rosa_laevigata RLG00000005998
rosa_multiflora Rmu_co8503207.1_g000002 Rmu_sc0025219.1_g000001 Rmu_ssc0000204.1_g000036
rosa_roxburghii Rroxscaffold_5G00383420
rosa_rugosa Rorug04G0341400
rosa_samantha Rh4AG394100 Rh4BG407100 Rh4CG422500 Rh4DG401100
rosa_wichuraiana Rw4G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1148
Acc65I GGTACC 1 cut(s) 711
AccB1I GGYRCC 1 cut(s) 711
AccII CGCG 1 cut(s) 1044
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 3 cut(s) 14, 24, 520
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 3 cut(s) 297, 757, 1142
AcuI CTGAAG 4 cut(s) 449, 470, 593, 660
AcvI CACGTG 1 cut(s) 111
AfaI GTAC 3 cut(s) 182, 713, 747
AfiI CCNNNNNNNGG 2 cut(s) 514, 761
AgsI TTSAA 5 cut(s) 168, 187, 307, 731, 962
AjnI CCWGG 2 cut(s) 480, 586
AjuI GAANNNNNNNTTGG 4 cut(s) 443, 475, 1076, 1108
Alw21I GWGCWC 1 cut(s) 1130
Alw26I GTCTC 2 cut(s) 208, 989
AlwNI CAGNNNCTG 1 cut(s) 556
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 5 cut(s) 41, 150, 377, 573, 1170
ApeKI GCWGC 4 cut(s) 172, 475, 547, 925
ApoI RAATTY 3 cut(s) 297, 757, 1142
Asp718I GGTACC 1 cut(s) 711
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 3 cut(s) 41, 151, 517
AsuHPI GGTGA 3 cut(s) 16, 659, 992
AvaII GGWCC 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 341
BalI TGGCCA 1 cut(s) 575
BanI GGYRCC 1 cut(s) 711
BanII GRGCYC 1 cut(s) 1130
BbrPI CACGTG 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 1130
BbvI GCAGC 4 cut(s) 159, 487, 559, 937
BccI CCATC 4 cut(s) 70, 388, 565, 794
BcgI CGANNNNNNTGC 4 cut(s) 116, 150, 151, 185
BciT130I CCWGG 2 cut(s) 482, 588
BcoDI GTCTC 2 cut(s) 208, 989
BfaI CTAG 6 cut(s) 204, 318, 566, 797, 939, 999
BfmI CTRYAG 1 cut(s) 867
BisI GCNGC 4 cut(s) 173, 476, 548, 926
BlsI GCNGC 4 cut(s) 174, 477, 549, 927
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 2 cut(s) 482, 588
Bme18I GGWCC 1 cut(s) 517
BmgT120I GGNCC 3 cut(s) 41, 151, 517
BmiI GGNNCC 5 cut(s) 42, 51, 518, 713, 1014
BmrFI CCNGG 2 cut(s) 482, 588
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 3 cut(s) 365, 543, 778
BmuI ACTGGG 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 437
Bpu10I CCTNAGC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 532
BsaAI YACGTR 1 cut(s) 111
BsaBI GATNNNNATC 2 cut(s) 252, 792
BsaJI CCNNGG 1 cut(s) 587
BsaWI WCCGGW 3 cut(s) 370, 778, 895
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 2 cut(s) 514, 761
Bse118I RCCGGY 2 cut(s) 43, 122
Bse1I ACTGG 2 cut(s) 154, 683
Bse8I GATNNNNATC 2 cut(s) 252, 792
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 2 cut(s) 482, 588
BseDI CCNNGG 1 cut(s) 587
BseGI GGATG 3 cut(s) 88, 328, 856
BseJI GATNNNNATC 2 cut(s) 252, 792
BseLI CCNNNNNNNGG 2 cut(s) 514, 761
BseMII CTCAG 4 cut(s) 73, 780, 984, 1094
BseNI ACTGG 2 cut(s) 154, 683
BseRI GAGGAG 1 cut(s) 42
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 4 cut(s) 159, 487, 559, 937
Bsh1236I CGCG 1 cut(s) 1044
BshFI GGCC 5 cut(s) 43, 152, 379, 575, 1172
BshNI GGYRCC 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 1130
BsiSI CCGG 5 cut(s) 44, 123, 371, 779, 896
BslFI GGGAC 2 cut(s) 530, 1176
BslI CCNNNNNNNGG 2 cut(s) 514, 761
BsmAI GTCTC 2 cut(s) 208, 989
BsmFI GGGAC 2 cut(s) 530, 1176
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 5 cut(s) 43, 152, 379, 575, 1172
Bsp1286I GDGCHC 2 cut(s) 341, 1130
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 3 cut(s) 253, 343, 1027
BspACI CCGC 3 cut(s) 14, 24, 520
BspANI GGCC 5 cut(s) 43, 152, 379, 575, 1172
BspCNI CTCAG 4 cut(s) 72, 779, 985, 1093
BspEI TCCGGA 1 cut(s) 370
BspFNI CGCG 1 cut(s) 1044
BspHI TCATGA 2 cut(s) 210, 834
BspLI GGNNCC 5 cut(s) 42, 51, 518, 713, 1014
BspQI GCTCTTC 1 cut(s) 978
BspT107I GGYRCC 1 cut(s) 711
BsrFI RCCGGY 2 cut(s) 43, 122
BsrI ACTGG 2 cut(s) 154, 683
BssAI RCCGGY 2 cut(s) 43, 122
BssECI CCNNGG 1 cut(s) 587
BssMI GATC 3 cut(s) 253, 343, 1027
Bst2UI CCWGG 2 cut(s) 482, 588
Bst4CI ACNGT 4 cut(s) 194, 663, 698, 1105
Bst6I CTCTTC 1 cut(s) 978
BstAPI GCANNNNNTGC 1 cut(s) 553
BstBAI YACGTR 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 990
BstDEI CTNAG 5 cut(s) 59, 470, 766, 993, 1080
BstF5I GGATG 3 cut(s) 88, 328, 856
BstFNI CGCG 1 cut(s) 1044
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 3 cut(s) 256, 346, 1030
BstMAI GTCTC 2 cut(s) 208, 989
BstMBI GATC 3 cut(s) 253, 343, 1027
BstMWI GCNNNNNNNGC 3 cut(s) 475, 553, 610
BstNI CCWGG 2 cut(s) 482, 588
BstNSI RCATGY 2 cut(s) 746, 992
BstSCI CCNGG 2 cut(s) 480, 586
BstSFI CTRYAG 1 cut(s) 867
BstSLI GKGCMC 1 cut(s) 341
BstUI CGCG 1 cut(s) 1044
BstV1I GCAGC 4 cut(s) 159, 487, 559, 937
BstV2I GAAGAC 1 cut(s) 437
BsuRI GGCC 5 cut(s) 43, 152, 379, 575, 1172
BtsCI GGATG 3 cut(s) 88, 328, 856
BtsIMutI CAGTG 2 cut(s) 668, 690
Cac8I GCNNGC 1 cut(s) 990
CaiI CAGNNNCTG 1 cut(s) 556
CciI TCATGA 2 cut(s) 210, 834
CfoI GCGC 1 cut(s) 507
Cfr10I RCCGGY 2 cut(s) 43, 122
Cfr13I GGNCC 3 cut(s) 41, 151, 517
Csp6I GTAC 3 cut(s) 181, 712, 746
CviAII CATG 9 cut(s) 148, 211, 577, 743, 835, 918, 989, 1159, 1186
CviQI GTAC 3 cut(s) 181, 712, 746
DdeI CTNAG 5 cut(s) 59, 470, 766, 993, 1080
DpnI GATC 3 cut(s) 255, 345, 1029
DpnII GATC 3 cut(s) 253, 343, 1027
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
EciI GGCGGA 2 cut(s) 29, 39
Ecl136II GAGCTC 1 cut(s) 1128
Eco24I GRGCYC 1 cut(s) 1130
Eco47I GGWCC 1 cut(s) 517
Eco53kI GAGCTC 1 cut(s) 1128
Eco57I CTGAAG 4 cut(s) 449, 470, 593, 660
Eco72I CACGTG 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 1128
EcoRI GAATTC 1 cut(s) 757
EcoRII CCWGG 2 cut(s) 480, 586
EcoT38I GRGCYC 1 cut(s) 1130
FaeI CATG 9 cut(s) 151, 214, 580, 746, 838, 921, 992, 1162, 1189
FaqI GGGAC 2 cut(s) 530, 1176
FatI CATG 9 cut(s) 147, 210, 576, 742, 834, 917, 988, 1158, 1185
Fnu4HI GCNGC 4 cut(s) 173, 476, 548, 926
FokI GGATG 3 cut(s) 95, 315, 863
FriOI GRGCYC 1 cut(s) 1130
Fsp4HI GCNGC 4 cut(s) 173, 476, 548, 926
FspBI CTAG 6 cut(s) 204, 318, 566, 797, 939, 999
GlaI GCGC 1 cut(s) 506
GluI GCNGC 4 cut(s) 173, 476, 548, 926
HaeIII GGCC 5 cut(s) 43, 152, 379, 575, 1172
HapII CCGG 5 cut(s) 44, 123, 371, 779, 896
HhaI GCGC 1 cut(s) 507
Hin1II CATG 9 cut(s) 151, 214, 580, 746, 838, 921, 992, 1162, 1189
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 7 cut(s) 98, 248, 541, 782, 812, 1097, 1178
HpaII CCGG 5 cut(s) 44, 123, 371, 779, 896
HphI GGTGA 3 cut(s) 16, 659, 992
Hpy188III TCNNGA 9 cut(s) 160, 211, 371, 511, 797, 835, 878, 1031, 1200
HpyAV CCTTC 4 cut(s) 13, 26, 269, 956
HpyCH4III ACNGT 4 cut(s) 194, 663, 698, 1105
HpyCH4IV ACGT 2 cut(s) 110, 1205
HpyCH4V TGCA 4 cut(s) 269, 580, 917, 1091
HpyF10VI GCNNNNNNNGC 3 cut(s) 475, 553, 610
HpyF3I CTNAG 5 cut(s) 59, 470, 766, 993, 1080
HpySE526I ACGT 2 cut(s) 110, 1205
Hsp92II CATG 9 cut(s) 151, 214, 580, 746, 838, 921, 992, 1162, 1189
HspAI GCGC 1 cut(s) 505
Kpn2I TCCGGA 1 cut(s) 370
KpnI GGTACC 1 cut(s) 715
Kzo9I GATC 3 cut(s) 253, 343, 1027
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 3 cut(s) 55, 922, 1112
Lsp1109I GCAGC 4 cut(s) 159, 487, 559, 937
LweI GCATC 3 cut(s) 365, 543, 778
MaeI CTAG 6 cut(s) 204, 318, 566, 797, 939, 999
MaeII ACGT 2 cut(s) 110, 1205
MaeIII GTNAC 1 cut(s) 194
MalI GATC 3 cut(s) 255, 345, 1029
MboI GATC 3 cut(s) 253, 343, 1027
MboII GAAGA 7 cut(s) 319, 442, 550, 599, 821, 995, 1084
MhlI GDGCHC 2 cut(s) 341, 1130
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 8 cut(s) 297, 417, 458, 625, 633, 757, 1036, 1142
MluNI TGGCCA 1 cut(s) 575
MlyI GAGTC 2 cut(s) 92, 1172
MnlI CCTC 9 cut(s) 20, 281, 315, 405, 507, 518, 702, 1000, 1183
Mox20I TGGCCA 1 cut(s) 575
MroI TCCGGA 1 cut(s) 370
MscI TGGCCA 1 cut(s) 575
MslI CAYNNNNRTG 1 cut(s) 839
Msp20I TGGCCA 1 cut(s) 575
MspI CCGG 5 cut(s) 44, 123, 371, 779, 896
MspR9I CCNGG 2 cut(s) 482, 588
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 2 cut(s) 482, 588
MvnI CGCG 1 cut(s) 1044
MwoI GCNNNNNNNGC 3 cut(s) 475, 553, 610
NdeII GATC 3 cut(s) 253, 343, 1027
NlaIII CATG 9 cut(s) 151, 214, 580, 746, 838, 921, 992, 1162, 1189
NlaIV GGNNCC 5 cut(s) 42, 51, 518, 713, 1014
NspI RCATGY 2 cut(s) 746, 992
PaeI GCATGC 1 cut(s) 992
PagI TCATGA 2 cut(s) 210, 834
PciSI GCTCTTC 1 cut(s) 978
PctI GAATGC 1 cut(s) 829
PfeI GAWTC 5 cut(s) 248, 541, 782, 812, 1097
PkrI GCNGC 4 cut(s) 174, 477, 549, 927
PleI GAGTC 2 cut(s) 92, 1172
PmaCI CACGTG 1 cut(s) 111
PmlI CACGTG 1 cut(s) 111
PpsI GAGTC 2 cut(s) 92, 1172
Ppu21I YACGTR 1 cut(s) 111
PsiI TTATAA 1 cut(s) 1148
Psp124BI GAGCTC 1 cut(s) 1130
Psp6I CCWGG 2 cut(s) 480, 586
PspCI CACGTG 1 cut(s) 111
PspGI CCWGG 2 cut(s) 480, 586
PspN4I GGNNCC 5 cut(s) 42, 51, 518, 713, 1014
PspPI GGNCC 3 cut(s) 41, 151, 517
PstNI CAGNNNCTG 1 cut(s) 556
RsaI GTAC 3 cut(s) 182, 713, 747
RsaNI GTAC 3 cut(s) 181, 712, 746
RseI CAYNNNNRTG 1 cut(s) 839
SacI GAGCTC 1 cut(s) 1130
SapI GCTCTTC 1 cut(s) 978
SatI GCNGC 4 cut(s) 173, 476, 548, 926
Sau3AI GATC 3 cut(s) 253, 343, 1027
Sau96I GGNCC 3 cut(s) 41, 151, 517
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 2 cut(s) 92, 1172
ScrFI CCNGG 2 cut(s) 482, 588
SduI GDGCHC 2 cut(s) 341, 1130
SfaNI GCATC 3 cut(s) 365, 543, 778
SfcI CTRYAG 1 cut(s) 867
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 839
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
SphI GCATGC 1 cut(s) 992
Sse9I AATT 8 cut(s) 297, 417, 458, 625, 633, 757, 1036, 1142
SsiI CCGC 3 cut(s) 14, 24, 520
SspI AATATT 2 cut(s) 313, 958
SspMI CTAG 6 cut(s) 204, 318, 566, 797, 939, 999
SstI GAGCTC 1 cut(s) 1130
StyD4I CCNGG 2 cut(s) 480, 586
TaaI ACNGT 4 cut(s) 194, 663, 698, 1105
TaiI ACGT 2 cut(s) 113, 1208
TaqI TCGA 3 cut(s) 161, 286, 1062
TasI AATT 8 cut(s) 297, 417, 458, 625, 633, 757, 1036, 1142
TatI WGTACW 2 cut(s) 180, 745
TfiI GAWTC 5 cut(s) 248, 541, 782, 812, 1097
TscAI CASTG 2 cut(s) 668, 690
TseI GCWGC 4 cut(s) 172, 475, 547, 925
TspDTI ATGAA 5 cut(s) 199, 823, 851, 867, 1085
TspRI CASTG 2 cut(s) 668, 690
VpaK11BI GGWCC 1 cut(s) 517
XapI RAATTY 3 cut(s) 297, 757, 1142
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 2 cut(s) 746, 992
XspI CTAG 6 cut(s) 204, 318, 566, 797, 939, 999
ZrmI AGTACT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.