RLG00000005998

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012430
Physical Location & Seq
Forward (+)
3393760 .. 3396285
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005998

Sequence Viewer

Length: 1497 bp
ATGGTGAGAAAATCGAAGGAGAAGGAGAAGACCGGAGATGGGTCCGGCTCCGCCGAGGGATCGGTGGCTCCGTTTCTGAGAAAATGTTACGAGATGGTCGACGACGAAGAAACCGACTCTATAATCTCGTGGAGTGAGAGCAATGGCAGCTTTGTGATTAGGGACGTGACCCGGTTCTCGGTTTTGATGCTCCCCAAGTATTTCAAGCACAGCAACTTTTCTAGCTTCATGAGGCAGCTCAATATCTACGGCTTTAGAAAAATAGATCCGGATCATTATGTATTTGCAAATGAAGGATTTGTTCGAGGTCAAAAGCATTTGTTGAAGAATATTTTTAGAAGGAAATATCCGCAGGGCACAGATCAGAGAAAAACATTGCAGCAGAAAGACAATCAGAGAGACGATCCTGATGAACCTTCTGAAGACATTGAAAGTGGTCTGTGGAGGGAAGTTGAGAACCTTAAGATCGATAAGATCTCTCTAAAGCAAGAGTTGGTGAAGCTTAGGCAGCACCAGGAAATATCAGAAAATAACTTGCTCCTCCTGCGAGATCGCCTTCATGGAATGGAGAAGGATCAGCAACAGATGCTCTCATTTTTGGTGATGGCAATGCAAAATCCTGGGTTTTTAGTTCAGCTGGTTCAGTCAAAAGAACATAGCTGGCGCTTCGCTGAAGCTGGAAATATGCTAGAAGAAGTAGATGATGGTAGACCAATGGCTGCTGATGGTGCCATAGTAAGGTACCAACCTCCTATGGATGAAGCACCAGAGCCTGTCTTCACACCACATTCAGGTTCGGAGATTCAACCAGAAATAGATTCTTTTGTGAATTCTGATGGTGTAATAGTAAGGTACCAACCTGTGGATGAAGCGCCAGATCCTGTATTCACACCGAATTCTGTCTCAGAGAAACAACCAGAATTTGATTCTTATCCCGATGGAGTGGGAGATTCTTTCATGAATTCTGATGGTGCAATAGTAAGGTACCGATCAGCTATGGATGCAGCACCAAAGCCTATACTCACACAGAGTTCAGGCTCAGGAAAACAACCAGAGTTTGATTCTTACCCTGATGGGATGAAAGATTTTTTTGTGAATTCTGATTTAGTGAAAATGCTAATGGATGATAAGTTGAGCCCTCGAGAAAATCATGCCCCATTTATCCTGCCAGATGCTGATGTTGATGATAATGCATGGGAGAAGCTTCTTTTAACTAGTTATTTGTTAGAAAATACTGAAGGTGCAAAGGAAGAAAGAAAAGAGCCCGAGGATTCTAGAATGGAGGTCGAATCAACAGCAGCTGAGTTGGACGAATCCAAAAATTTTGATTCTTTAATAGAACAAATGAACAAATCAGTTCATGGATCTAATAAGGAGAACTCTCAGAATTTGGAATTCATTGCTTATAATAGGATAACTATAGCCCCAGTCCATCTTTCTTACACAAGTAACAAATATTTTGATGGCTACAATCTTTTGGGAGACCAACATGTTTAA

Protein Analysis

499

Amino Acids

56.84

Weight (kDa)

4.79

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 25 - 114 3.6e-27 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67970
fragaria_vesca FvH4_4g33360 FvH4_4g33360
malus_domestica MD13G1018800.v1.1 MD16G1016700.v1.1
prunus_persica Prupe.1G335100_v2.0.a1
pyrus_communis pycom13g01590
rosa_chinensis RchiOBHm_Chr4g0442621
rosa_laevigata RLG00000005998
rosa_multiflora Rmu_co8503207.1_g000002 Rmu_sc0025219.1_g000001 Rmu_ssc0000204.1_g000036
rosa_roxburghii Rroxscaffold_5G00383420
rosa_rugosa Rorug04G0341400
rosa_samantha Rh4AG394100 Rh4BG407100 Rh4CG422500 Rh4DG401100
rosa_wichuraiana Rw4G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1407
Acc65I GGTACC 3 cut(s) 741, 852, 984
AccB1I GGYRCC 4 cut(s) 728, 741, 852, 984
AccB7I CCANNNNNTGG 1 cut(s) 862
AccI GTMKAC 2 cut(s) 99, 709
AccIII TCCGGA 1 cut(s) 268
AciI CCGC 2 cut(s) 51, 350
AclWI GGATC 7 cut(s) 67, 260, 279, 398, 582, 872, 1372
AcsI RAATTY 8 cut(s) 829, 895, 920, 961, 1096, 1321, 1387, 1394
AcuI CTGAAG 3 cut(s) 441, 693, 1257
AfaI GTAC 3 cut(s) 743, 854, 986
AfiI CCNNNNNNNGG 5 cut(s) 39, 178, 738, 791, 862
AflII CTTAAG 1 cut(s) 461
AflIII ACRYGT 1 cut(s) 1489
AgsI TTSAA 4 cut(s) 205, 325, 431, 806
AhlI ACTAGT 1 cut(s) 1214
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 2 cut(s) 513, 619
Alw26I GTCTC 3 cut(s) 393, 907, 1476
AlwI GGATC 7 cut(s) 67, 260, 279, 398, 582, 872, 1372
AlwNI CAGNNNCTG 4 cut(s) 773, 881, 1175, 1301
Ama87I CYCGRG 2 cut(s) 1140, 1265
Aor13HI TCCGGA 1 cut(s) 268
ApeKI GCWGC 7 cut(s) 147, 235, 379, 508, 719, 1004, 1298
ApoI RAATTY 8 cut(s) 829, 895, 920, 961, 1096, 1321, 1387, 1394
Asp718I GGTACC 3 cut(s) 741, 852, 984
AspLEI GCGC 2 cut(s) 666, 874
AspS9I GGNCC 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 3 cut(s) 16, 508, 613
AvaI CYCGRG 2 cut(s) 1140, 1265
AvaII GGWCC 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 359
BanI GGYRCC 4 cut(s) 728, 741, 852, 984
BanII GRGCYC 2 cut(s) 1139, 1266
BauI CACGAG 1 cut(s) 127
BbsI GAAGAC 3 cut(s) 35, 429, 769
BbvI GCAGC 7 cut(s) 159, 247, 391, 520, 706, 1016, 1310
BceAI ACGGC 1 cut(s) 265
BciT130I CCWGG 2 cut(s) 515, 621
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 3 cut(s) 393, 907, 1476
BcuI ACTAGT 1 cut(s) 1214
BfaI CTAG 4 cut(s) 222, 689, 1215, 1275
BfmI CTRYAG 1 cut(s) 1419
BfoI RGCGCY 2 cut(s) 667, 875
BfrI CTTAAG 1 cut(s) 461
BglII AGATCT 1 cut(s) 474
BisI GCNGC 7 cut(s) 148, 236, 380, 509, 720, 1005, 1299
BlsI GCNGC 7 cut(s) 149, 237, 381, 510, 721, 1006, 1300
Bme1390I CCNGG 3 cut(s) 172, 515, 621
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 2 cut(s) 1140, 1265
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 7 cut(s) 43, 49, 69, 730, 743, 854, 986
BmrFI CCNGG 3 cut(s) 172, 515, 621
BmrI ACTGGG 1 cut(s) 1421
BmsI GCATC 4 cut(s) 177, 576, 991, 1162
BmuI ACTGGG 1 cut(s) 1421
BpiI GAAGAC 3 cut(s) 35, 429, 769
Bpu10I CCTNAGC 2 cut(s) 503, 1039
BpuMI CCSGG 1 cut(s) 172
Bsa29I ATCGAT 1 cut(s) 468
BsaBI GATNNNNATC 2 cut(s) 270, 930
BsaI GGTCTC 1 cut(s) 1476
BsaJI CCNNGG 3 cut(s) 54, 620, 1266
BsaWI WCCGGW 2 cut(s) 32, 268
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 178, 738, 791, 862
Bse1I ACTGG 1 cut(s) 1427
Bse3DI GCAATG 4 cut(s) 148, 374, 615, 1398
Bse8I GATNNNNATC 2 cut(s) 270, 930
BseAI TCCGGA 1 cut(s) 268
BseBI CCWGG 2 cut(s) 515, 621
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 54, 620, 1266
BseGI GGATG 5 cut(s) 763, 871, 1006, 1083, 1129
BseJI GATNNNNATC 2 cut(s) 270, 930
BseLI CCNNNNNNNGG 5 cut(s) 39, 178, 738, 791, 862
BseMI GCAATG 4 cut(s) 148, 374, 615, 1398
BseMII CTCAG 5 cut(s) 68, 918, 1053, 1293, 1397
BseNI ACTGG 1 cut(s) 1427
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 1 cut(s) 359
BseXI GCAGC 7 cut(s) 159, 247, 391, 520, 706, 1016, 1310
BshNI GGYRCC 4 cut(s) 728, 741, 852, 984
BshVI ATCGAT 1 cut(s) 468
BsiHKCI CYCGRG 2 cut(s) 1140, 1265
BsiSI CCGG 4 cut(s) 33, 45, 172, 269
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 5 cut(s) 39, 178, 738, 791, 862
BsmAI GTCTC 3 cut(s) 393, 907, 1476
BsmBI CGTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 176
Bso31I GGTCTC 1 cut(s) 1476
BsoBI CYCGRG 2 cut(s) 1140, 1265
Bsp1286I GDGCHC 3 cut(s) 359, 1139, 1266
Bsp13I TCCGGA 1 cut(s) 268
BspACI CCGC 2 cut(s) 51, 350
BspCNI CTCAG 5 cut(s) 69, 917, 1052, 1294, 1396
BspDI ATCGAT 1 cut(s) 468
BspEI TCCGGA 1 cut(s) 268
BspHI TCATGA 2 cut(s) 228, 957
BspLI GGNNCC 7 cut(s) 43, 49, 69, 730, 743, 854, 986
BspPI GGATC 7 cut(s) 67, 260, 279, 398, 582, 872, 1372
BspT107I GGYRCC 4 cut(s) 728, 741, 852, 984
BspTI CTTAAG 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 1476
BsrDI GCAATG 4 cut(s) 148, 374, 615, 1398
BsrI ACTGG 1 cut(s) 1427
BssECI CCNNGG 3 cut(s) 54, 620, 1266
BssSI CACGAG 1 cut(s) 127
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 2 cut(s) 515, 621
BstAFI CTTAAG 1 cut(s) 461
BstAPI GCANNNNNTGC 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 662
BstDEI CTNAG 6 cut(s) 77, 503, 904, 1039, 1302, 1383
BstF5I GGATG 5 cut(s) 763, 871, 1006, 1083, 1129
BstH2I RGCGCY 2 cut(s) 667, 875
BstHHI GCGC 2 cut(s) 666, 874
BstMAI GTCTC 3 cut(s) 393, 907, 1476
BstMWI GCNNNNNNNGC 6 cut(s) 147, 508, 544, 586, 728, 1001
BstNI CCWGG 2 cut(s) 515, 621
BstNSI RCATGY 1 cut(s) 1493
BstSCI CCNGG 3 cut(s) 170, 513, 619
BstSFI CTRYAG 1 cut(s) 1419
BstSLI GKGCMC 1 cut(s) 359
BstV1I GCAGC 7 cut(s) 159, 247, 391, 520, 706, 1016, 1310
BstV2I GAAGAC 3 cut(s) 35, 429, 769
BstX2I RGATCY 4 cut(s) 265, 474, 877, 1364
BstYI RGATCY 4 cut(s) 265, 474, 877, 1364
Bsu15I ATCGAT 1 cut(s) 468
BsuTUI ATCGAT 1 cut(s) 468
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 5 cut(s) 763, 871, 1006, 1083, 1129
Cac8I GCNNGC 1 cut(s) 662
CaiI CAGNNNCTG 4 cut(s) 773, 881, 1175, 1301
CciI TCATGA 2 cut(s) 228, 957
CfoI GCGC 2 cut(s) 666, 874
Cfr13I GGNCC 1 cut(s) 42
ClaI ATCGAT 1 cut(s) 468
Csp6I GTAC 3 cut(s) 742, 853, 985
CviAII CATG 7 cut(s) 229, 560, 958, 1151, 1194, 1361, 1490
CviQI GTAC 3 cut(s) 742, 853, 985
DdeI CTNAG 6 cut(s) 77, 503, 904, 1039, 1302, 1383
EciI GGCGGA 1 cut(s) 40
Eco24I GRGCYC 2 cut(s) 1139, 1266
Eco31I GGTCTC 1 cut(s) 1476
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 3 cut(s) 441, 693, 1257
Eco88I CYCGRG 2 cut(s) 1140, 1265
EcoRI GAATTC 5 cut(s) 829, 895, 961, 1096, 1394
EcoRII CCWGG 2 cut(s) 513, 619
EcoT22I ATGCAT 1 cut(s) 1195
EcoT38I GRGCYC 2 cut(s) 1139, 1266
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 7 cut(s) 232, 563, 961, 1154, 1197, 1364, 1493
FaqI GGGAC 1 cut(s) 176
FatI CATG 7 cut(s) 228, 559, 957, 1150, 1193, 1360, 1489
FblI GTMKAC 2 cut(s) 99, 709
Fnu4HI GCNGC 7 cut(s) 148, 236, 380, 509, 720, 1005, 1299
FokI GGATG 5 cut(s) 770, 878, 1013, 1090, 1136
FriOI GRGCYC 2 cut(s) 1139, 1266
Fsp4HI GCNGC 7 cut(s) 148, 236, 380, 509, 720, 1005, 1299
FspBI CTAG 4 cut(s) 222, 689, 1215, 1275
GlaI GCGC 2 cut(s) 665, 873
GluI GCNGC 7 cut(s) 148, 236, 380, 509, 720, 1005, 1299
HaeII RGCGCY 2 cut(s) 667, 875
HapII CCGG 4 cut(s) 33, 45, 172, 269
HhaI GCGC 2 cut(s) 666, 874
Hin1II CATG 7 cut(s) 232, 563, 961, 1154, 1197, 1364, 1493
Hin6I GCGC 2 cut(s) 664, 872
HinP1I GCGC 2 cut(s) 664, 872
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 2 cut(s) 500, 1202
HpaII CCGG 4 cut(s) 33, 45, 172, 269
HphI GGTGA 3 cut(s) 16, 508, 613
Hpy166II GTNNAC 2 cut(s) 100, 710
Hpy188III TCNNGA 8 cut(s) 229, 269, 407, 935, 958, 1041, 1142, 1275
Hpy8I GTNNAC 2 cut(s) 100, 710
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 8 cut(s) 10, 16, 287, 333, 426, 565, 566, 1232
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 7 cut(s) 287, 379, 613, 974, 1004, 1193, 1244
HpyF10VI GCNNNNNNNGC 6 cut(s) 147, 508, 544, 586, 728, 1001
HpyF3I CTNAG 6 cut(s) 77, 503, 904, 1039, 1302, 1383
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 7 cut(s) 232, 563, 961, 1154, 1197, 1364, 1493
HspAI GCGC 2 cut(s) 664, 872
Kpn2I TCCGGA 1 cut(s) 268
KpnI GGTACC 3 cut(s) 745, 856, 988
LmnI GCTCC 4 cut(s) 53, 73, 195, 543
Lsp1109I GCAGC 7 cut(s) 159, 247, 391, 520, 706, 1016, 1310
LweI GCATC 4 cut(s) 177, 576, 991, 1162
MaeI CTAG 4 cut(s) 222, 689, 1215, 1275
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 3 cut(s) 86, 166, 1448
MboII GAAGA 7 cut(s) 40, 119, 337, 434, 704, 769, 1262
MflI RGATCY 4 cut(s) 265, 474, 877, 1364
MhlI GDGCHC 3 cut(s) 359, 1139, 1266
MluCI AATT 8 cut(s) 829, 895, 920, 961, 1096, 1321, 1387, 1394
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 1287
MnlI CCTC 9 cut(s) 49, 225, 299, 438, 551, 759, 1149, 1261, 1276
Mph1103I ATGCAT 1 cut(s) 1195
MroI TCCGGA 1 cut(s) 268
MseI TTAA 4 cut(s) 462, 1211, 1334, 1495
MspA1I CMGCKG 2 cut(s) 637, 1301
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 4 cut(s) 33, 45, 172, 269
MspR9I CCNGG 3 cut(s) 172, 515, 621
MvaI CCWGG 2 cut(s) 515, 621
MwoI GCNNNNNNNGC 6 cut(s) 147, 508, 544, 586, 728, 1001
NciI CCSGG 1 cut(s) 172
NlaIII CATG 7 cut(s) 232, 563, 961, 1154, 1197, 1364, 1493
NlaIV GGNNCC 7 cut(s) 43, 49, 69, 730, 743, 854, 986
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 1195
NspI RCATGY 1 cut(s) 1493
PaeR7I CTCGAG 1 cut(s) 1140
PagI TCATGA 2 cut(s) 228, 957
PciI ACATGT 1 cut(s) 1489
PcsI WCGNNNNNNNCGW 3 cut(s) 68, 96, 111
PfeI GAWTC 9 cut(s) 802, 818, 926, 950, 1061, 1271, 1289, 1313, 1328
PflMI CCANNNNNTGG 1 cut(s) 862
PkrI GCNGC 7 cut(s) 149, 237, 381, 510, 721, 1006, 1300
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PscI ACATGT 1 cut(s) 1489
PsiI TTATAA 1 cut(s) 1407
Psp6I CCWGG 2 cut(s) 513, 619
PspGI CCWGG 2 cut(s) 513, 619
PspN4I GGNNCC 7 cut(s) 43, 49, 69, 730, 743, 854, 986
PspPI GGNCC 1 cut(s) 42
PstNI CAGNNNCTG 4 cut(s) 773, 881, 1175, 1301
PsuI RGATCY 4 cut(s) 265, 474, 877, 1364
PvuII CAGCTG 2 cut(s) 637, 1301
RsaI GTAC 3 cut(s) 743, 854, 986
RsaNI GTAC 3 cut(s) 742, 853, 985
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 4 cut(s) 462, 1211, 1334, 1495
SatI GCNGC 7 cut(s) 148, 236, 380, 509, 720, 1005, 1299
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 3 cut(s) 172, 515, 621
SduI GDGCHC 3 cut(s) 359, 1139, 1266
SfaNI GCATC 4 cut(s) 177, 576, 991, 1162
SfcI CTRYAG 1 cut(s) 1419
Sfr274I CTCGAG 1 cut(s) 1140
SinI GGWCC 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 1140
SmlI CTYRAG 2 cut(s) 461, 1140
SmoI CTYRAG 2 cut(s) 461, 1140
SpeI ACTAGT 1 cut(s) 1214
Sse9I AATT 8 cut(s) 829, 895, 920, 961, 1096, 1321, 1387, 1394
SsiI CCGC 2 cut(s) 51, 350
SspI AATATT 2 cut(s) 331, 1457
SspMI CTAG 4 cut(s) 222, 689, 1215, 1275
StyD4I CCNGG 3 cut(s) 170, 513, 619
TaiI ACGT 1 cut(s) 168
TaqI TCGA 6 cut(s) 14, 99, 304, 468, 1141, 1287
TasI AATT 8 cut(s) 829, 895, 920, 961, 1096, 1321, 1387, 1394
TfiI GAWTC 9 cut(s) 802, 818, 926, 950, 1061, 1271, 1289, 1313, 1328
Tru1I TTAA 4 cut(s) 462, 1211, 1334, 1495
Tru9I TTAA 4 cut(s) 462, 1211, 1334, 1495
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 7 cut(s) 147, 235, 379, 508, 719, 1004, 1298
Tsp45I GTSAC 1 cut(s) 166
TspGWI ACGGA 1 cut(s) 60
Van91I CCANNNNNTGG 1 cut(s) 862
Vha464I CTTAAG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 8 cut(s) 829, 895, 920, 961, 1096, 1321, 1387, 1394
XbaI TCTAGA 1 cut(s) 1274
XceI RCATGY 1 cut(s) 1493
XhoI CTCGAG 1 cut(s) 1140
XmiI GTMKAC 2 cut(s) 99, 709
XspI CTAG 4 cut(s) 222, 689, 1215, 1275
Zsp2I ATGCAT 1 cut(s) 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.