MD16G1016700.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012430
Physical Location & Seq
Reverse (-)
1245839 .. 1248585
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1016700.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGGTGAAATCGGCGGAGAAGGGCGGAGGAGATGGGTCTGGGCCGTCGGGGTCGGTGCCTCCTTTTCTCAGAAAATGCTACGAGATGGTGGATGACAAGGACAGCGACAGTATAATCTCGTGGAGTGAAGCCGGCGACAGCTTTGCGATACTGGACATGGCCCAGTTCTCGATTTCAATGCTGCCCAAGTATTTCAAGCACAGCAACTTTTCTAGCTTCATGAGGCAGCTCAATATCTATGGATTTAGAAAAATAGATCCAGATCGTTGGGTGTTTGCAAATGAAGGATTTATTCGAGGTCAAAAGCATTTGTTGAAGAATATTGCTAGAAGGAAACATCCACAGGGCACAGATCAAAAAAAGATATTACAGCAAAAAGACAATCCTGATATACCGTCTGAAAATATTAGTGAAAATGGTCTGTGGAAGGAAGTTGAGAACTTGAAGACTGATAAAGTTGCTCTGAAGCAAGAGTTGGTGAAGCTTAGGCAGCACCAGGAAATTTCACAAAATAAATTGCTCCTCCTGAGGAACCGCCTTCGCGGAATGGAGAAAAATCAACAGCAGATGCTGTCATTTCTAGTGATGGCCATGCAAAGTCCTGGATTTCTAGTTCAGCTTCTTCATCCAAAGGAAAATAGTTGGCGAATTGCTGAAGCTGGCAATATAATAGAACAGTGTATGGATGATGATAGACCAGTGGCTTCTGATGGTGCGATAGTGAGGTACCAACCACCGATGATTGAAGCCCCAAAGCCTTTAGTCCCACCGAATTCAGGCTCAGAGAAACAACCTGAAGTTGATGCTTATATGGATGGAATGGAAGATTTTGTTGTAAATCCTGATTTCATGAAAATGCTAATGGATGAAAAGTTGAGCCCTGTAGAAACCCATGCCCCATATACTCTACCAGATATATCTGACGATGGTGCATGGGAGCAGTTTCTTTTAGCTAGTCCTTTCTTAGAAAATATTGAAGGTACAAAGGAAGATGGCGAAGAGCCTGCTGATGCTAGAATGGAGGTGGAACCAATAGTATCTGATCTGGATGAATCACAAAATTTTGATTATTTAGTCGAGCAAATGAAGAAATCTCAGAACTTTGCATCGGATTCAACAGTTGATGGATCTAATATGGAGAGCTCTCAAAACTTGGAGTTTATTACTGAACAAATGGGACATTTGGCTTCTGACTCTAACAACATGGAACAGAATCAGGGAAGCAGAAGGACAACTTCTGTGTAA

Protein Analysis

415

Amino Acids

46.88

Weight (kDa)

4.8

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 22 - 111 2.6e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67970
fragaria_vesca FvH4_4g33360 FvH4_4g33360
malus_domestica MD13G1018800.v1.1 MD16G1016700.v1.1
prunus_persica Prupe.1G335100_v2.0.a1
pyrus_communis pycom13g01590
rosa_chinensis RchiOBHm_Chr4g0442621
rosa_laevigata RLG00000005998
rosa_multiflora Rmu_co8503207.1_g000002 Rmu_sc0025219.1_g000001 Rmu_ssc0000204.1_g000036
rosa_roxburghii Rroxscaffold_5G00383420
rosa_rugosa Rorug04G0341400
rosa_samantha Rh4AG394100 Rh4BG407100 Rh4CG422500 Rh4DG401100
rosa_wichuraiana Rw4G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 726
AccB1I GGYRCC 2 cut(s) 55, 726
AccII CGCG 1 cut(s) 543
AciI CCGC 4 cut(s) 14, 24, 535, 543
AclWI GGATC 2 cut(s) 251, 1135
AcoI YGGCCR 1 cut(s) 588
AcsI RAATTY 3 cut(s) 501, 772, 1060
AcuI CTGAAG 3 cut(s) 485, 675, 816
AfaI GTAC 2 cut(s) 728, 982
AfiI CCNNNNNNNGG 1 cut(s) 776
AgsI TTSAA 7 cut(s) 177, 196, 316, 445, 746, 977, 1116
AjnI CCWGG 2 cut(s) 495, 601
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AloI GAACNNNNNNTCC 2 cut(s) 597, 629
AluBI AGCT 8 cut(s) 141, 216, 229, 484, 619, 659, 953, 1143
AluI AGCT 8 cut(s) 141, 216, 229, 484, 619, 659, 953, 1143
Alw21I GWGCWC 1 cut(s) 1145
AlwI GGATC 2 cut(s) 251, 1135
AlwNI CAGNNNCTG 1 cut(s) 571
AoxI GGCC 3 cut(s) 41, 159, 588
ApeKI GCWGC 3 cut(s) 181, 226, 490
ApoI RAATTY 3 cut(s) 501, 772, 1060
Asp718I GGTACC 1 cut(s) 726
AspS9I GGNCC 2 cut(s) 41, 160
AsuHPI GGTGA 2 cut(s) 16, 490
AxyI CCTNAGG 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 350
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 2 cut(s) 55, 726
BanII GRGCYC 2 cut(s) 881, 1145
BauI CACGAG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 1145
BbvI GCAGC 3 cut(s) 168, 238, 502
BccI CCATC 8 cut(s) 26, 79, 580, 704, 809, 920, 986, 1118
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 6 cut(s) 125, 159, 160, 194, 986, 1020
BciT130I CCWGG 2 cut(s) 497, 603
BfaI CTAG 6 cut(s) 213, 327, 581, 611, 954, 1014
BfmI CTRYAG 1 cut(s) 882
BisI GCNGC 3 cut(s) 182, 227, 491
BlsI GCNGC 3 cut(s) 183, 228, 492
Bme1390I CCNGG 2 cut(s) 497, 603
BmgT120I GGNCC 2 cut(s) 41, 160
BmiI GGNNCC 4 cut(s) 57, 533, 728, 1029
BmrFI CCNGG 2 cut(s) 497, 603
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 4 cut(s) 558, 793, 1000, 1115
BmuI ACTGGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 485
BsaBI GATNNNNATC 1 cut(s) 261
Bsc4I CCNNNNNNNGG 1 cut(s) 776
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 3 cut(s) 156, 163, 698
Bse21I CCTNAGG 1 cut(s) 527
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 2 cut(s) 497, 603
BseGI GGATG 7 cut(s) 97, 337, 625, 691, 820, 871, 1054
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 776
BseMII CTCAG 4 cut(s) 82, 518, 795, 1109
BseNI ACTGG 3 cut(s) 156, 163, 698
BseRI GAGGAG 2 cut(s) 42, 512
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 3 cut(s) 168, 238, 502
Bsh1236I CGCG 1 cut(s) 543
BshFI GGCC 3 cut(s) 43, 161, 590
BshNI GGYRCC 2 cut(s) 55, 726
BsiHKAI GWGCWC 1 cut(s) 1145
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 2 cut(s) 749, 1191
BslI CCNNNNNNNGG 1 cut(s) 776
BsmFI GGGAC 2 cut(s) 749, 1191
BsnI GGCC 3 cut(s) 43, 161, 590
Bsp1286I GDGCHC 3 cut(s) 350, 881, 1145
Bsp143I GATC 5 cut(s) 256, 262, 352, 1042, 1127
BspACI CCGC 4 cut(s) 14, 24, 535, 543
BspANI GGCC 3 cut(s) 43, 161, 590
BspCNI CTCAG 4 cut(s) 81, 519, 794, 1108
BspFNI CGCG 1 cut(s) 543
BspHI TCATGA 2 cut(s) 219, 849
BspLI GGNNCC 4 cut(s) 57, 533, 728, 1029
BspPI GGATC 2 cut(s) 251, 1135
BspQI GCTCTTC 1 cut(s) 993
BspT107I GGYRCC 2 cut(s) 55, 726
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 3 cut(s) 156, 163, 698
BssAI RCCGGY 1 cut(s) 131
BssMI GATC 5 cut(s) 256, 262, 352, 1042, 1127
BssSI CACGAG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 497, 603
Bst4CI ACNGT 4 cut(s) 110, 396, 678, 1120
Bst6I CTCTTC 1 cut(s) 993
BstC8I GCNNGC 3 cut(s) 133, 661, 1005
BstDEI CTNAG 6 cut(s) 68, 485, 527, 781, 964, 1095
BstF5I GGATG 7 cut(s) 97, 337, 625, 691, 820, 871, 1054
BstFNI CGCG 1 cut(s) 543
BstKTI GATC 5 cut(s) 259, 265, 355, 1045, 1130
BstMBI GATC 5 cut(s) 256, 262, 352, 1042, 1127
BstMWI GCNNNNNNNGC 1 cut(s) 490
BstNI CCWGG 2 cut(s) 497, 603
BstSCI CCNGG 2 cut(s) 495, 601
BstSFI CTRYAG 1 cut(s) 882
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 543
BstV1I GCAGC 3 cut(s) 168, 238, 502
BstV2I GAAGAC 1 cut(s) 452
BstX2I RGATCY 2 cut(s) 256, 1127
BstXI CCANNNNNNTGG 1 cut(s) 267
BstYI RGATCY 2 cut(s) 256, 1127
Bsu36I CCTNAGG 1 cut(s) 527
BsuRI GGCC 3 cut(s) 43, 161, 590
BtsCI GGATG 7 cut(s) 97, 337, 625, 691, 820, 871, 1054
BtsIMutI CAGTG 2 cut(s) 683, 705
Cac8I GCNNGC 3 cut(s) 133, 661, 1005
CaiI CAGNNNCTG 1 cut(s) 571
CciI TCATGA 2 cut(s) 219, 849
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 41, 160
Csp6I GTAC 2 cut(s) 727, 981
CviAII CATG 7 cut(s) 157, 220, 592, 850, 893, 933, 1204
CviQI GTAC 2 cut(s) 727, 981
DdeI CTNAG 6 cut(s) 68, 485, 527, 781, 964, 1095
DpnI GATC 5 cut(s) 258, 264, 354, 1044, 1129
DpnII GATC 5 cut(s) 256, 262, 352, 1042, 1127
EaeI YGGCCR 1 cut(s) 588
Eam1104I CTCTTC 1 cut(s) 993
EarI CTCTTC 1 cut(s) 993
EciI GGCGGA 2 cut(s) 29, 39
Ecl136II GAGCTC 1 cut(s) 1143
Eco24I GRGCYC 2 cut(s) 881, 1145
Eco53kI GAGCTC 1 cut(s) 1143
Eco57I CTGAAG 3 cut(s) 485, 675, 816
Eco81I CCTNAGG 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 1143
EcoRI GAATTC 1 cut(s) 772
EcoRII CCWGG 2 cut(s) 495, 601
EcoT38I GRGCYC 2 cut(s) 881, 1145
FaeI CATG 7 cut(s) 160, 223, 595, 853, 896, 936, 1207
FalI AAGNNNNNCTT 1 cut(s) 1219
FaqI GGGAC 2 cut(s) 749, 1191
FatI CATG 7 cut(s) 156, 219, 591, 849, 892, 932, 1203
Fnu4HI GCNGC 3 cut(s) 182, 227, 491
FokI GGATG 7 cut(s) 104, 324, 612, 698, 827, 878, 1061
FriOI GRGCYC 2 cut(s) 881, 1145
Fsp4HI GCNGC 3 cut(s) 182, 227, 491
FspBI CTAG 6 cut(s) 213, 327, 581, 611, 954, 1014
GluI GCNGC 3 cut(s) 182, 227, 491
HaeIII GGCC 3 cut(s) 43, 161, 590
HapII CCGG 1 cut(s) 132
Hin1II CATG 7 cut(s) 160, 223, 595, 853, 896, 936, 1207
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 4 cut(s) 1052, 1112, 1193, 1213
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 16, 490
Hpy188III TCNNGA 8 cut(s) 169, 220, 260, 386, 526, 842, 850, 1046
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 7 cut(s) 13, 278, 324, 421, 548, 971, 1221
HpyCH4III ACNGT 4 cut(s) 110, 396, 678, 1120
HpyCH4V TGCA 4 cut(s) 278, 595, 932, 1106
HpyF10VI GCNNNNNNNGC 1 cut(s) 490
HpyF3I CTNAG 6 cut(s) 68, 485, 527, 781, 964, 1095
Hsp92II CATG 7 cut(s) 160, 223, 595, 853, 896, 936, 1207
KpnI GGTACC 1 cut(s) 730
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Kzo9I GATC 5 cut(s) 256, 262, 352, 1042, 1127
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 2 cut(s) 525, 937
Lsp1109I GCAGC 3 cut(s) 168, 238, 502
LweI GCATC 4 cut(s) 558, 793, 1000, 1115
MaeI CTAG 6 cut(s) 213, 327, 581, 611, 954, 1014
MalI GATC 5 cut(s) 258, 264, 354, 1044, 1129
MboI GATC 5 cut(s) 256, 262, 352, 1042, 1127
MboII GAAGA 7 cut(s) 328, 457, 614, 836, 1001, 1010, 1099
MflI RGATCY 2 cut(s) 256, 1127
MhlI GDGCHC 3 cut(s) 350, 881, 1145
MlsI TGGCCA 1 cut(s) 590
MluCI AATT 5 cut(s) 501, 515, 648, 772, 1060
MluNI TGGCCA 1 cut(s) 590
MlyI GAGTC 1 cut(s) 1187
MnlI CCTC 8 cut(s) 20, 69, 216, 290, 522, 533, 717, 1015
Mox20I TGGCCA 1 cut(s) 590
MroNI GCCGGC 1 cut(s) 131
MscI TGGCCA 1 cut(s) 590
MslI CAYNNNNRTG 1 cut(s) 854
Msp20I TGGCCA 1 cut(s) 590
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 497, 603
MvaI CCWGG 2 cut(s) 497, 603
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 1 cut(s) 490
NaeI GCCGGC 1 cut(s) 133
NdeII GATC 5 cut(s) 256, 262, 352, 1042, 1127
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 7 cut(s) 160, 223, 595, 853, 896, 936, 1207
NlaIV GGNNCC 4 cut(s) 57, 533, 728, 1029
PagI TCATGA 2 cut(s) 219, 849
PciSI GCTCTTC 1 cut(s) 993
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 3 cut(s) 1052, 1112, 1213
PfoI TCCNGGA 1 cut(s) 601
PkrI GCNGC 3 cut(s) 183, 228, 492
PleI GAGTC 1 cut(s) 1187
PpsI GAGTC 1 cut(s) 1187
Psp124BI GAGCTC 1 cut(s) 1145
Psp6I CCWGG 2 cut(s) 495, 601
PspGI CCWGG 2 cut(s) 495, 601
PspN4I GGNNCC 4 cut(s) 57, 533, 728, 1029
PspPI GGNCC 2 cut(s) 41, 160
PstNI CAGNNNCTG 1 cut(s) 571
PsuI RGATCY 2 cut(s) 256, 1127
RsaI GTAC 2 cut(s) 728, 982
RsaNI GTAC 2 cut(s) 727, 981
RseI CAYNNNNRTG 1 cut(s) 854
SacI GAGCTC 1 cut(s) 1145
SapI GCTCTTC 1 cut(s) 993
SatI GCNGC 3 cut(s) 182, 227, 491
Sau3AI GATC 5 cut(s) 256, 262, 352, 1042, 1127
Sau96I GGNCC 2 cut(s) 41, 160
SchI GAGTC 1 cut(s) 1187
ScrFI CCNGG 2 cut(s) 497, 603
SduI GDGCHC 3 cut(s) 350, 881, 1145
SfaNI GCATC 4 cut(s) 558, 793, 1000, 1115
SfcI CTRYAG 1 cut(s) 882
SmiMI CAYNNNNRTG 1 cut(s) 854
Sse9I AATT 5 cut(s) 501, 515, 648, 772, 1060
SsiI CCGC 4 cut(s) 14, 24, 535, 543
SspI AATATT 3 cut(s) 322, 406, 973
SspMI CTAG 6 cut(s) 213, 327, 581, 611, 954, 1014
SstI GAGCTC 1 cut(s) 1145
StyD4I CCNGG 2 cut(s) 495, 601
TaaI ACNGT 4 cut(s) 110, 396, 678, 1120
TaqI TCGA 3 cut(s) 170, 295, 1077
TasI AATT 5 cut(s) 501, 515, 648, 772, 1060
TfiI GAWTC 3 cut(s) 1052, 1112, 1213
TscAI CASTG 2 cut(s) 683, 705
TseI GCWGC 3 cut(s) 181, 226, 490
TspDTI ATGAA 8 cut(s) 208, 297, 614, 838, 866, 882, 1065, 1100
TspRI CASTG 2 cut(s) 683, 705
XapI RAATTY 3 cut(s) 501, 772, 1060
XspI CTAG 6 cut(s) 213, 327, 581, 611, 954, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.