Prupe.1G335100_v2.0.a1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012430
Physical Location & Seq
Forward (+)
31664746 .. 31667517
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G335100.1

Sequence Viewer

Length: 1227 bp
ATGTTGAAATCGGCGGTGAAGAGTGGAGATGGGTCTGGGTCTGGTGGATCGGTGGCTCCTTTTCTTAGGAAATGCTATGAGATGGTGGATGATAACGATGCAGACTCTATAATCTCGTGGAGTGAAACCGGTGACAGCTTTGTGATATGGGACATGACCCAGTTCTCGATTTTATTGTTGCCCAAGTATTTCAAGCACAGCAACTTTTCTAGCTTCATGAGGCAGCTCAATATCTATGGCTTCAGAAAAATAGATTCAGATCGTTGGGTGTTTGCAAATGAAGGGTTTATTCGAGGTCAAAAGCATTTGTTGAAGAATATTTCTAGAAGGAAACATCCTCAGGGCACAGATCAGAGAAAAACATTACAGCAGAAAGACAATCCTGATGGGCCTTGTGAAAACATTGTTGAAAATGGTCTATGGAGGGAAGTTGAGAACCTGAAGACGGATAAAGTTGCTCTGAAGCAAGAGTTGGTCAAGCTTAGGCAGCACCAGGAAATTTCAGAAAATAACTTGCTCCTCCTGAGGAACCGCCTTCATGGAATGGAGAAGAATCAGCAGCAGATGCTGTCATTTCTAGTTATGGCCATGCAAAGTCCTGGGTTTTTAGTTCAGCTTCTTCAGCCAAAAGAAAACAGTTGGCGCATTGCTGAACCTGGGAATATGCTAGAACAAGGTGTAGATGATGGTATACCAATAACTTCTGATGGTGCGATAGTGAGGTACCAACCTCCTGTGGATGAAGCCCCGAAGCCTGTCCTCGCAGCGAATTCAGGCTCAGACAAACAAACCGAATTTGATTCTTATATAGATGGAATGAACGATTTTGTTGTGAATCCTGATTTCATGAAAATGCTAATGGATGAAAAGTTGAGCTCTCTAGAAAATCAAGCCCCATATACCCTACCGGATGTATCTGATGATGGTGCATGGGAGCAGCTTCTTTTAGCTAGTCCTTTCTTAGAAGATATTGAAGCTGCAAAGGAAGACGGAAAAGAGACTGTTGACTCTAGAATGGAGGTGGAATCAACCGCATCGGAACTGCAAGAATCACAGAATTTTGATACTTTAATAGAGCAAATGAAGAAATCTCAGAACTTTGCATCAGAATCAACAGTTTATGGATCTAATGTGGAGAGCTCTCAAAGCTTGGAACATATAACTGAACAAATGGGATATTTAGCTTCTGACTCTAACAGCAAACGTGGGACACAATCAGGAAAGTGA

Protein Analysis

409

Amino Acids

46.02

Weight (kDa)

4.81

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67970
fragaria_vesca FvH4_4g33360 FvH4_4g33360
malus_domestica MD13G1018800.v1.1 MD16G1016700.v1.1
prunus_persica Prupe.1G335100_v2.0.a1
pyrus_communis pycom13g01590
rosa_chinensis RchiOBHm_Chr4g0442621
rosa_laevigata RLG00000005998
rosa_multiflora Rmu_co8503207.1_g000002 Rmu_sc0025219.1_g000001 Rmu_ssc0000204.1_g000036
rosa_roxburghii Rroxscaffold_5G00383420
rosa_rugosa Rorug04G0341400
rosa_samantha Rh4AG394100 Rh4BG407100 Rh4CG422500 Rh4DG401100
rosa_wichuraiana Rw4G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 723
AccB1I GGYRCC 1 cut(s) 723
AccI GTMKAC 1 cut(s) 691
AciI CCGC 3 cut(s) 14, 532, 1032
AclWI GGATC 2 cut(s) 55, 1132
AcoI YGGCCR 1 cut(s) 585
AcsI RAATTY 4 cut(s) 498, 769, 794, 1057
AcuI CTGAAG 4 cut(s) 226, 461, 482, 605
AfaI GTAC 1 cut(s) 725
AfiI CCNNNNNNNGG 1 cut(s) 445
AgeI ACCGGT 1 cut(s) 128
AgsI TTSAA 5 cut(s) 7, 193, 313, 410, 974
AjnI CCWGG 3 cut(s) 492, 598, 655
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
Alw21I GWGCWC 2 cut(s) 878, 1142
Alw26I GTCTC 1 cut(s) 992
AlwI GGATC 2 cut(s) 55, 1132
AlwNI CAGNNNCTG 1 cut(s) 568
AoxI GGCC 2 cut(s) 389, 585
ApeKI GCWGC 6 cut(s) 223, 487, 559, 764, 937, 977
ApoI RAATTY 4 cut(s) 498, 769, 794, 1057
AsiGI ACCGGT 1 cut(s) 128
Asp718I GGTACC 1 cut(s) 723
AspLEI GCGC 1 cut(s) 645
AspS9I GGNCC 1 cut(s) 389
AsuHPI GGTGA 2 cut(s) 28, 143
AxyI CCTNAGG 2 cut(s) 339, 524
BaeGI GKGCMC 1 cut(s) 347
BalI TGGCCA 1 cut(s) 587
BanI GGYRCC 1 cut(s) 723
BanII GRGCYC 2 cut(s) 878, 1142
BauI CACGAG 1 cut(s) 115
BbsI GAAGAC 2 cut(s) 449, 993
Bbv12I GWGCWC 2 cut(s) 878, 1142
BbvI GCAGC 6 cut(s) 235, 499, 571, 776, 949, 964
BccI CCATC 7 cut(s) 23, 76, 380, 680, 701, 806, 917
BciT130I CCWGG 3 cut(s) 494, 600, 657
BcoDI GTCTC 1 cut(s) 992
BfaI CTAG 7 cut(s) 210, 324, 578, 668, 881, 951, 1011
BisI GCNGC 6 cut(s) 224, 488, 560, 765, 938, 978
BlsI GCNGC 6 cut(s) 225, 489, 561, 766, 939, 979
Bme1390I CCNGG 3 cut(s) 494, 600, 657
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 3 cut(s) 57, 530, 725
BmrFI CCNGG 3 cut(s) 494, 600, 657
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 4 cut(s) 88, 555, 1043, 1112
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 2 cut(s) 449, 993
Bpu10I CCTNAGC 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 599, 656
BsaWI WCCGGW 2 cut(s) 128, 907
Bsc4I CCNNNNNNNGG 1 cut(s) 445
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 160
Bse21I CCTNAGG 2 cut(s) 339, 524
Bse3DI GCAATG 1 cut(s) 645
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 3 cut(s) 494, 600, 657
BseDI CCNNGG 2 cut(s) 599, 656
BseGI GGATG 5 cut(s) 94, 334, 745, 868, 916
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 445
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 4 cut(s) 353, 515, 792, 1106
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 1 cut(s) 509
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 6 cut(s) 235, 499, 571, 776, 949, 964
BshFI GGCC 2 cut(s) 391, 587
BshNI GGYRCC 1 cut(s) 723
BshTI ACCGGT 1 cut(s) 128
BsiHKAI GWGCWC 2 cut(s) 878, 1142
BsiSI CCGG 2 cut(s) 129, 908
BslFI GGGAC 2 cut(s) 164, 1222
BslI CCNNNNNNNGG 1 cut(s) 445
BsmAI GTCTC 1 cut(s) 992
BsmFI GGGAC 2 cut(s) 164, 1222
BsnI GGCC 2 cut(s) 391, 587
Bsp1286I GDGCHC 3 cut(s) 347, 878, 1142
Bsp143I GATC 4 cut(s) 47, 259, 349, 1124
BspACI CCGC 3 cut(s) 14, 532, 1032
BspANI GGCC 2 cut(s) 391, 587
BspCNI CTCAG 4 cut(s) 352, 516, 791, 1105
BspHI TCATGA 2 cut(s) 216, 846
BspLI GGNNCC 3 cut(s) 57, 530, 725
BspPI GGATC 2 cut(s) 55, 1132
BspT107I GGYRCC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 1 cut(s) 160
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 2 cut(s) 599, 656
BssMI GATC 4 cut(s) 47, 259, 349, 1124
BssNAI GTATAC 1 cut(s) 692
BssSI CACGAG 1 cut(s) 115
Bst1107I GTATAC 1 cut(s) 692
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 3 cut(s) 494, 600, 657
Bst4CI ACNGT 3 cut(s) 638, 1003, 1117
Bst6I CTCTTC 1 cut(s) 14
BstAPI GCANNNNNTGC 1 cut(s) 565
BstDEI CTNAG 7 cut(s) 65, 339, 482, 524, 778, 961, 1092
BstF5I GGATG 5 cut(s) 94, 334, 745, 868, 916
BstHHI GCGC 1 cut(s) 645
BstKTI GATC 4 cut(s) 50, 262, 352, 1127
BstMAI GTCTC 1 cut(s) 992
BstMBI GATC 4 cut(s) 47, 259, 349, 1124
BstMWI GCNNNNNNNGC 4 cut(s) 487, 565, 622, 1146
BstNI CCWGG 3 cut(s) 494, 600, 657
BstSCI CCNGG 3 cut(s) 492, 598, 655
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 6 cut(s) 235, 499, 571, 776, 949, 964
BstV2I GAAGAC 2 cut(s) 449, 993
BstX2I RGATCY 1 cut(s) 1124
BstYI RGATCY 1 cut(s) 1124
BstZ17I GTATAC 1 cut(s) 692
Bsu36I CCTNAGG 2 cut(s) 339, 524
BsuRI GGCC 2 cut(s) 391, 587
BtsCI GGATG 5 cut(s) 94, 334, 745, 868, 916
CaiI CAGNNNCTG 1 cut(s) 568
CciI TCATGA 2 cut(s) 216, 846
CfoI GCGC 1 cut(s) 645
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 724
CspAI ACCGGT 1 cut(s) 128
CviAII CATG 6 cut(s) 154, 217, 539, 589, 847, 930
CviQI GTAC 1 cut(s) 724
DdeI CTNAG 7 cut(s) 65, 339, 482, 524, 778, 961, 1092
DpnI GATC 4 cut(s) 49, 261, 351, 1126
DpnII GATC 4 cut(s) 47, 259, 349, 1124
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Ecl136II GAGCTC 2 cut(s) 876, 1140
Eco24I GRGCYC 2 cut(s) 878, 1142
Eco53kI GAGCTC 2 cut(s) 876, 1140
Eco57I CTGAAG 4 cut(s) 226, 461, 482, 605
Eco81I CCTNAGG 2 cut(s) 339, 524
EcoICRI GAGCTC 2 cut(s) 876, 1140
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 3 cut(s) 492, 598, 655
EcoT38I GRGCYC 2 cut(s) 878, 1142
FaeI CATG 6 cut(s) 157, 220, 542, 592, 850, 933
FaqI GGGAC 2 cut(s) 164, 1222
FatI CATG 6 cut(s) 153, 216, 538, 588, 846, 929
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 6 cut(s) 224, 488, 560, 765, 938, 978
FokI GGATG 5 cut(s) 101, 321, 752, 875, 923
FriOI GRGCYC 2 cut(s) 878, 1142
Fsp4HI GCNGC 6 cut(s) 224, 488, 560, 765, 938, 978
FspBI CTAG 7 cut(s) 210, 324, 578, 668, 881, 951, 1011
GlaI GCGC 1 cut(s) 644
GluI GCNGC 6 cut(s) 224, 488, 560, 765, 938, 978
HaeIII GGCC 2 cut(s) 391, 587
HapII CCGG 2 cut(s) 129, 908
HhaI GCGC 1 cut(s) 645
Hin1II CATG 6 cut(s) 157, 220, 542, 592, 850, 933
Hin6I GCGC 1 cut(s) 643
HinP1I GCGC 1 cut(s) 643
HincII GTYRAC 1 cut(s) 1006
HindII GTYRAC 1 cut(s) 1006
HindIII AAGCTT 2 cut(s) 479, 1147
HpaII CCGG 2 cut(s) 129, 908
HphI GGTGA 2 cut(s) 28, 143
Hpy166II GTNNAC 2 cut(s) 692, 1006
Hpy8I GTNNAC 2 cut(s) 692, 1006
HpyAV CCTTC 3 cut(s) 275, 321, 545
HpyCH4III ACNGT 3 cut(s) 638, 1003, 1117
HpyCH4IV ACGT 1 cut(s) 1204
HpyCH4V TGCA 7 cut(s) 101, 275, 592, 929, 980, 1045, 1103
HpyF10VI GCNNNNNNNGC 4 cut(s) 487, 565, 622, 1146
HpyF3I CTNAG 7 cut(s) 65, 339, 482, 524, 778, 961, 1092
HpySE526I ACGT 1 cut(s) 1204
Hsp92II CATG 6 cut(s) 157, 220, 542, 592, 850, 933
HspAI GCGC 1 cut(s) 643
KpnI GGTACC 1 cut(s) 727
Kzo9I GATC 4 cut(s) 47, 259, 349, 1124
LmnI GCTCC 3 cut(s) 61, 522, 934
Lsp1109I GCAGC 6 cut(s) 235, 499, 571, 776, 949, 964
LweI GCATC 4 cut(s) 88, 555, 1043, 1112
MaeI CTAG 7 cut(s) 210, 324, 578, 668, 881, 951, 1011
MaeII ACGT 1 cut(s) 1204
MaeIII GTNAC 1 cut(s) 131
MalI GATC 4 cut(s) 49, 261, 351, 1126
MboI GATC 4 cut(s) 47, 259, 349, 1124
MboII GAAGA 8 cut(s) 31, 325, 454, 562, 611, 977, 998, 1096
MflI RGATCY 1 cut(s) 1124
MhlI GDGCHC 3 cut(s) 347, 878, 1142
MlsI TGGCCA 1 cut(s) 587
MluCI AATT 4 cut(s) 498, 769, 794, 1057
MluNI TGGCCA 1 cut(s) 587
MlyI GAGTC 3 cut(s) 98, 1001, 1184
Mox20I TGGCCA 1 cut(s) 587
MscI TGGCCA 1 cut(s) 587
MseI TTAA 1 cut(s) 1070
MslI CAYNNNNRTG 1 cut(s) 851
Msp20I TGGCCA 1 cut(s) 587
MspI CCGG 2 cut(s) 129, 908
MspR9I CCNGG 3 cut(s) 494, 600, 657
MvaI CCWGG 3 cut(s) 494, 600, 657
MwoI GCNNNNNNNGC 4 cut(s) 487, 565, 622, 1146
NdeII GATC 4 cut(s) 47, 259, 349, 1124
NlaIII CATG 6 cut(s) 157, 220, 542, 592, 850, 933
NlaIV GGNNCC 3 cut(s) 57, 530, 725
NmuCI GTSAC 1 cut(s) 131
PagI TCATGA 2 cut(s) 216, 846
PfeI GAWTC 7 cut(s) 254, 553, 800, 835, 1025, 1049, 1109
PinAI ACCGGT 1 cut(s) 128
PkrI GCNGC 6 cut(s) 225, 489, 561, 766, 939, 979
PleI GAGTC 3 cut(s) 98, 1001, 1184
PpsI GAGTC 3 cut(s) 98, 1001, 1184
Psp124BI GAGCTC 2 cut(s) 878, 1142
Psp6I CCWGG 3 cut(s) 492, 598, 655
PspGI CCWGG 3 cut(s) 492, 598, 655
PspN4I GGNNCC 3 cut(s) 57, 530, 725
PspPI GGNCC 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 568
PsuI RGATCY 1 cut(s) 1124
RsaI GTAC 1 cut(s) 725
RsaNI GTAC 1 cut(s) 724
RseI CAYNNNNRTG 1 cut(s) 851
SacI GAGCTC 2 cut(s) 878, 1142
SaqAI TTAA 1 cut(s) 1070
SatI GCNGC 6 cut(s) 224, 488, 560, 765, 938, 978
Sau3AI GATC 4 cut(s) 47, 259, 349, 1124
Sau96I GGNCC 1 cut(s) 389
SchI GAGTC 3 cut(s) 98, 1001, 1184
ScrFI CCNGG 3 cut(s) 494, 600, 657
SduI GDGCHC 3 cut(s) 347, 878, 1142
SfaNI GCATC 4 cut(s) 88, 555, 1043, 1112
SmiMI CAYNNNNRTG 1 cut(s) 851
Sse9I AATT 4 cut(s) 498, 769, 794, 1057
SsiI CCGC 3 cut(s) 14, 532, 1032
SspI AATATT 1 cut(s) 319
SspMI CTAG 7 cut(s) 210, 324, 578, 668, 881, 951, 1011
SstI GAGCTC 2 cut(s) 878, 1142
StyD4I CCNGG 3 cut(s) 492, 598, 655
TaaI ACNGT 3 cut(s) 638, 1003, 1117
TaiI ACGT 1 cut(s) 1207
TaqI TCGA 2 cut(s) 167, 292
TasI AATT 4 cut(s) 498, 769, 794, 1057
TfiI GAWTC 7 cut(s) 254, 553, 800, 835, 1025, 1049, 1109
Tru1I TTAA 1 cut(s) 1070
Tru9I TTAA 1 cut(s) 1070
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 6 cut(s) 223, 487, 559, 764, 937, 977
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 9 cut(s) 205, 294, 527, 756, 833, 835, 863, 879, 1097
TspGWI ACGGA 2 cut(s) 461, 1005
XapI RAATTY 4 cut(s) 498, 769, 794, 1057
XbaI TCTAGA 3 cut(s) 323, 880, 1010
XmiI GTMKAC 1 cut(s) 691
XspI CTAG 7 cut(s) 210, 324, 578, 668, 881, 951, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.