MD13G1029600.v1.1

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009865
Physical Location & Seq
Forward (+)
2136186 .. 2138839
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1029600.v1.1.491

Sequence Viewer

Length: 867 bp
ATGATGTCTTCTGTATTTGTTTTCCTACTTACTGTTTCCTTCCTCGCAACTCCATCATCCTCCGCAACCACCTCCTTCGTCTTCGGCGGCTGTTCTCAGCAAAAATACCTCCCGGGTTCACCCTACGAGTCGAAAGTCAACTCGGTCCTCACTTCCCTGGGCAACTCAGCCATGTTCTCCACCTACAACAACTTCACCTTCCCCGGCAGCAGCTCCGCCGGCTCCCAGGACACCCTTTACGGCCTCTTCCAATGCCGCGGCGACCTCAGCAGCAATGACTGCTCCCAGTGCGTCGCTCGCGCGGCGAGTCAACTCGGCACCCTATGCGTCAACTCCTGTGGCGGCGCGTTGCAGCTCGAGGGCTGCTTGGTGAAGTACGACAACTCGAAGTTCTTAGGGGTGGAGGACAAGACACTGGTGGTTAAGAAATGTGGGCCGTCGAATGGGTTTGACTCGGATGCGTTGACCGACCTGGAAGCCGTGCTGGCGAATCTGGGGACCGGAGATGGGACCTACAAGCCATACAGGGTTAGCGGCTCAGGGAATGTTCGGGGCGTGGCGCAGTGTGTCGGGGATTTGAGTCCGAGCGAATGCCAGGATTGTCTGTCGGGAGCCGTCGGACAGCTCAGGTCCGGGTGCGGGACCAGCTCGTGGGGCGACATATTCTTGGCTAAGTGCTACGCGCGCTACTTGGAAGGGGGATATAACTCGAACGACGGTCATGATCATGATGATGACGATAACAATGACGATCTTGACAAGACACTTGCAATACTCATCGGAATCGTAGCAGCCGCTGCACTGCTCACCGTATTTATTCACCACTTCTGGAAAAAATTGGACCGCGATGAAAAAGGTTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0030054 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

289

Amino Acids

30.43

Weight (kDa)

4.84

Isoelectric Point (pI)

29.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 31 - 128 3.5e-19 Salt stress response/antifungal
Stress-antifung PF01657 156 - 230 1.9e-09 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01660 AT2G01660 AT2G01660
fragaria_vesca FvH4_4g33800 FvH4_4g33800 FvH4_4g33800
malus_domestica MD04G1025700.v1.1 MD13G1029600.v1.1 MD16G1020400.v1.1
prunus_persica Prupe.1G331900_v2.0.a1
pyrus_communis pycom16g01830
rosa_chinensis RchiOBHm_Chr4g0443311
rosa_laevigata RLG00000005939
rosa_multiflora Rmu_ssc0000122.1_g000006
rosa_roxburghii Rroxscaffold_5G00383890
rosa_rugosa Rorug04G0345500
rosa_samantha Rh4AG400400 Rh4BG412000 Rh4CG427400 Rh4DG407400
rosa_wichuraiana Rw4G034540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AccB7I CCANNNNNTGG 1 cut(s) 651
AccII CGCG 7 cut(s) 258, 300, 302, 347, 683, 685, 846
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 4 cut(s) 443, 507, 639, 651
AjnI CCWGG 4 cut(s) 156, 225, 471, 594
AluBI AGCT 4 cut(s) 213, 355, 625, 648
AluI AGCT 4 cut(s) 213, 355, 625, 648
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 2 cut(s) 112, 356
AoxI GGCC 2 cut(s) 241, 434
ApeKI GCWGC 7 cut(s) 207, 210, 270, 352, 363, 791, 797
AspLEI GCGC 5 cut(s) 302, 347, 562, 685, 687
AspS9I GGNCC 7 cut(s) 145, 434, 498, 510, 630, 642, 841
AsuC2I CCSGG 4 cut(s) 113, 114, 204, 634
AsuHPI GGTGA 5 cut(s) 111, 187, 382, 799, 812
AvaI CYCGRG 2 cut(s) 112, 356
AvaII GGWCC 6 cut(s) 145, 498, 510, 630, 642, 841
BanI GGYRCC 1 cut(s) 317
BauI CACGAG 1 cut(s) 649
BbsI GAAGAC 1 cut(s) 73
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 7 cut(s) 219, 222, 282, 350, 364, 784, 803
BccI CCATC 2 cut(s) 61, 500
BceAI ACGGC 4 cut(s) 256, 421, 464, 599
BciT130I CCWGG 4 cut(s) 158, 227, 473, 596
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 4 cut(s) 113, 114, 204, 634
BglI GCCNNNNNGGC 1 cut(s) 485
Bme1390I CCNGG 8 cut(s) 113, 114, 158, 204, 227, 473, 596, 634
Bme18I GGWCC 6 cut(s) 145, 498, 510, 630, 642, 841
BmeT110I CYCGRG 2 cut(s) 112, 356
BmgT120I GGNCC 7 cut(s) 145, 434, 498, 510, 630, 642, 841
BmiI GGNNCC 6 cut(s) 223, 319, 499, 511, 613, 643
BmrFI CCNGG 8 cut(s) 113, 114, 158, 204, 227, 473, 596, 634
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 448
BmuI ACTGGG 1 cut(s) 280
BpiI GAAGAC 1 cut(s) 73
Bpu10I CCTNAGC 3 cut(s) 266, 538, 626
BpuMI CCSGG 4 cut(s) 113, 114, 204, 634
BsaJI CCNNGG 6 cut(s) 112, 156, 157, 202, 225, 256
BsaWI WCCGGW 1 cut(s) 500
Bsc4I CCNNNNNNNGG 4 cut(s) 443, 507, 639, 651
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 286, 420
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 4 cut(s) 158, 227, 473, 596
BseDI CCNNGG 6 cut(s) 112, 156, 157, 202, 225, 256
BseGI GGATG 2 cut(s) 56, 463
BseLI CCNNNNNNNGG 4 cut(s) 443, 507, 639, 651
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 5 cut(s) 110, 180, 280, 552, 640
BseNI ACTGG 2 cut(s) 286, 420
BsePI GCGCGC 1 cut(s) 683
BseXI GCAGC 7 cut(s) 219, 222, 282, 350, 364, 784, 803
BsgI GTGCAG 1 cut(s) 783
Bsh1236I CGCG 7 cut(s) 258, 300, 302, 347, 683, 685, 846
BshFI GGCC 2 cut(s) 243, 436
BshNI GGYRCC 1 cut(s) 317
BsiHKCI CYCGRG 2 cut(s) 112, 356
BsiSI CCGG 5 cut(s) 113, 204, 219, 501, 633
BslFI GGGAC 3 cut(s) 511, 523, 655
BslI CCNNNNNNNGG 4 cut(s) 443, 507, 639, 651
BsmFI GGGAC 3 cut(s) 511, 523, 655
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 2 cut(s) 243, 436
BsoBI CYCGRG 2 cut(s) 112, 356
Bsp143I GATC 2 cut(s) 724, 751
BspANI GGCC 2 cut(s) 243, 436
BspCNI CTCAG 5 cut(s) 109, 179, 279, 551, 639
BspFNI CGCG 7 cut(s) 258, 300, 302, 347, 683, 685, 846
BspHI TCATGA 2 cut(s) 721, 727
BspLI GGNNCC 6 cut(s) 223, 319, 499, 511, 613, 643
BspT107I GGYRCC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 2 cut(s) 286, 420
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 6 cut(s) 112, 156, 157, 202, 225, 256
BssHII GCGCGC 1 cut(s) 683
BssMI GATC 2 cut(s) 724, 751
BssSI CACGAG 1 cut(s) 649
Bst2BI CACGAG 1 cut(s) 649
Bst2UI CCWGG 4 cut(s) 158, 227, 473, 596
Bst4CI ACNGT 3 cut(s) 34, 719, 811
Bst6I CTCTTC 1 cut(s) 251
BstAPI GCANNNNNTGC 3 cut(s) 279, 324, 797
BstC8I GCNNGC 4 cut(s) 220, 298, 486, 685
BstDEI CTNAG 7 cut(s) 96, 166, 266, 394, 538, 626, 672
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 2 cut(s) 56, 463
BstFNI CGCG 7 cut(s) 258, 300, 302, 347, 683, 685, 846
BstHHI GCGC 5 cut(s) 302, 347, 562, 685, 687
BstKTI GATC 2 cut(s) 727, 754
BstMBI GATC 2 cut(s) 724, 751
BstNI CCWGG 4 cut(s) 158, 227, 473, 596
BstSCI CCNGG 8 cut(s) 111, 112, 156, 202, 225, 471, 594, 632
BstUI CGCG 7 cut(s) 258, 300, 302, 347, 683, 685, 846
BstV1I GCAGC 7 cut(s) 219, 222, 282, 350, 364, 784, 803
BstV2I GAAGAC 1 cut(s) 73
BsuRI GGCC 2 cut(s) 243, 436
BtgI CCRYGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 861
BtsCI GGATG 2 cut(s) 56, 463
BtsI GCAGTG 2 cut(s) 569, 800
BtsIMutI CAGTG 4 cut(s) 293, 413, 569, 800
Cac8I GCNNGC 4 cut(s) 220, 298, 486, 685
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 2 cut(s) 721, 727
CfoI GCGC 5 cut(s) 302, 347, 562, 685, 687
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 7 cut(s) 145, 434, 498, 510, 630, 642, 841
Cfr42I CCGCGG 1 cut(s) 259
Cfr9I CCCGGG 1 cut(s) 112
CseI GACGC 2 cut(s) 280, 316
Csp6I GTAC 1 cut(s) 376
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 3 cut(s) 172, 722, 728
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 7 cut(s) 96, 166, 266, 394, 538, 626, 672
DpnI GATC 2 cut(s) 726, 753
DpnII GATC 2 cut(s) 724, 751
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 205
Eco47I GGWCC 6 cut(s) 145, 498, 510, 630, 642, 841
Eco88I CYCGRG 2 cut(s) 112, 356
EcoO109I RGGNCCY 1 cut(s) 510
EcoRII CCWGG 4 cut(s) 156, 225, 471, 594
FaeI CATG 3 cut(s) 175, 725, 731
FaiI YATR 7 cut(s) 173, 325, 523, 662, 705, 723, 729
FaqI GGGAC 3 cut(s) 511, 523, 655
FatI CATG 3 cut(s) 171, 721, 727
FauI CCCGC 1 cut(s) 632
FbaI TGATCA 1 cut(s) 724
FokI GGATG 2 cut(s) 43, 470
GlaI GCGC 5 cut(s) 301, 346, 561, 684, 686
HaeIII GGCC 2 cut(s) 243, 436
HapII CCGG 5 cut(s) 113, 204, 219, 501, 633
HgaI GACGC 2 cut(s) 280, 316
HhaI GCGC 5 cut(s) 302, 347, 562, 685, 687
Hin1II CATG 3 cut(s) 175, 725, 731
Hin6I GCGC 5 cut(s) 300, 345, 560, 683, 685
HinP1I GCGC 5 cut(s) 300, 345, 560, 683, 685
HincII GTYRAC 4 cut(s) 139, 311, 331, 465
HindII GTYRAC 4 cut(s) 139, 311, 331, 465
HinfI GANTC 6 cut(s) 128, 307, 452, 490, 580, 783
HpaII CCGG 5 cut(s) 113, 204, 219, 501, 633
HphI GGTGA 5 cut(s) 111, 187, 382, 799, 812
Hpy166II GTNNAC 5 cut(s) 119, 139, 311, 331, 465
Hpy188I TCNGA 4 cut(s) 457, 585, 620, 782
Hpy188III TCNNGA 5 cut(s) 609, 722, 728, 755, 829
Hpy8I GTNNAC 5 cut(s) 119, 139, 311, 331, 465
Hpy99I CGWCG 4 cut(s) 296, 442, 620, 719
HpyAV CCTTC 4 cut(s) 49, 85, 208, 689
HpyCH4III ACNGT 3 cut(s) 34, 719, 811
HpyCH4V TGCA 3 cut(s) 352, 770, 800
HpyF3I CTNAG 7 cut(s) 96, 166, 266, 394, 538, 626, 672
Hsp92II CATG 3 cut(s) 175, 725, 731
HspAI GCGC 5 cut(s) 300, 345, 560, 683, 685
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 724
KspI CCGCGG 1 cut(s) 259
Kzo9I GATC 2 cut(s) 724, 751
LmnI GCTCC 4 cut(s) 218, 227, 287, 611
Lsp1109I GCAGC 7 cut(s) 219, 222, 282, 350, 364, 784, 803
LweI GCATC 1 cut(s) 448
MalI GATC 2 cut(s) 726, 753
MboI GATC 2 cut(s) 724, 751
MboII GAAGA 2 cut(s) 73, 238
MluCI AATT 1 cut(s) 836
MlyI GAGTC 4 cut(s) 137, 316, 446, 589
MmeI TCCRAC 1 cut(s) 598
MnlI CCTC 9 cut(s) 53, 70, 82, 119, 158, 254, 275, 352, 397
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 2 cut(s) 726, 732
MspA1I CMGCKG 2 cut(s) 258, 797
MspI CCGG 5 cut(s) 113, 204, 219, 501, 633
MspR9I CCNGG 8 cut(s) 113, 114, 158, 204, 227, 473, 596, 634
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 4 cut(s) 158, 227, 473, 596
MvnI CGCG 7 cut(s) 258, 300, 302, 347, 683, 685, 846
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 4 cut(s) 113, 114, 204, 634
NdeII GATC 2 cut(s) 724, 751
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 3 cut(s) 175, 725, 731
NlaIV GGNNCC 6 cut(s) 223, 319, 499, 511, 613, 643
NmeAIII GCCGAG 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 356
PagI TCATGA 2 cut(s) 721, 727
PasI CCCWGGG 1 cut(s) 157
PauI GCGCGC 1 cut(s) 683
PctI GAATGC 1 cut(s) 596
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 2 cut(s) 490, 783
PflMI CCANNNNNTGG 1 cut(s) 651
PleI GAGTC 4 cut(s) 136, 315, 446, 588
PpsI GAGTC 4 cut(s) 136, 315, 446, 588
PpuMI RGGWCCY 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 510
Psp6I CCWGG 4 cut(s) 156, 225, 471, 594
PspGI CCWGG 4 cut(s) 156, 225, 471, 594
PspN4I GGNNCC 6 cut(s) 223, 319, 499, 511, 613, 643
PspPI GGNCC 7 cut(s) 145, 434, 498, 510, 630, 642, 841
PspPPI RGGWCCY 1 cut(s) 510
PspXI VCTCGAGB 1 cut(s) 356
PstNI CAGNNNCTG 1 cut(s) 797
PteI GCGCGC 1 cut(s) 683
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 2 cut(s) 726, 732
SacII CCGCGG 1 cut(s) 259
SaqAI TTAA 1 cut(s) 423
Sau3AI GATC 2 cut(s) 724, 751
Sau96I GGNCC 7 cut(s) 145, 434, 498, 510, 630, 642, 841
SchI GAGTC 4 cut(s) 137, 316, 446, 589
ScrFI CCNGG 8 cut(s) 113, 114, 158, 204, 227, 473, 596, 634
SfaNI GCATC 1 cut(s) 448
Sfr274I CTCGAG 1 cut(s) 356
Sfr303I CCGCGG 1 cut(s) 259
SgrBI CCGCGG 1 cut(s) 259
SinI GGWCC 6 cut(s) 145, 498, 510, 630, 642, 841
SlaI CTCGAG 1 cut(s) 356
SmaI CCCGGG 1 cut(s) 114
SmiMI CAYNNNNRTG 2 cut(s) 726, 732
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 1 cut(s) 836
StyD4I CCNGG 8 cut(s) 111, 112, 156, 202, 225, 471, 594, 632
TaaI ACNGT 3 cut(s) 34, 719, 811
TaqI TCGA 5 cut(s) 131, 357, 386, 440, 710
TaqII GACCGA 2 cut(s) 133, 482
TasI AATT 1 cut(s) 836
TauI GCSGC 7 cut(s) 90, 258, 261, 305, 345, 537, 797
TfiI GAWTC 2 cut(s) 490, 783
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 4 cut(s) 293, 420, 569, 807
TseI GCWGC 7 cut(s) 207, 210, 270, 352, 363, 791, 797
TspDTI ATGAA 1 cut(s) 864
TspMI CCCGGG 1 cut(s) 112
TspRI CASTG 4 cut(s) 293, 420, 569, 807
Van91I CCANNNNNTGG 1 cut(s) 651
VpaK11BI GGWCC 6 cut(s) 145, 498, 510, 630, 642, 841
XhoI CTCGAG 1 cut(s) 356
XmaI CCCGGG 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.