MD16G1020400.v1.1

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009865
Physical Location & Seq
Forward (+)
1470130 .. 1472699
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1020400.v1.1.491

Sequence Viewer

Length: 867 bp
ATGATGTCTTCTGTATTTGTTTTCCTCCTCACTATTTCCTTCCTTACAACCCTGTCATCCTCCGCAACCACTTCCTTCGTCTTCGGCGGCTGTTCCCAGCAAAAATACCTCCCGGGTTCGCCCTACGAGTCGAAAGTAAACTCGCTCCTCACCTCCCTGGTCAACTCAGCCATGTTCTCTACCTACAACAACTTCACCTTCCCCGGTAGCAGCTCCGCCGGCTCCCAGGACACCCTATATGGCCTCTTCCAATGCCGTGGCGACCTCAGCAACATTGACTGCTCCCAGTGCGTCGCTCGCTCGGTGAGTCAACTCGGAACCCTCTGCCTCGACTCCTGCGGTGGCGCGTTGCAGCTCGAGGGCTGCTTTGTGAAGTACGACAACGCAACGTTCTTAGGGGTGGAGGACAAGACCGTGGTTGTTAAGAAATGTGGACCGTCGACTGGGTTTGACTCGGATTCGTTGACCAAGCGGGATGCCGTGCTGGCGTATCTTGGGACCAGCGATGGGACTTACAGGCCGTACAGAGTCAGCGGCTCCGGGAGTGTTCAGGGCGTGGCGCAGTGTATCGGGGATTTGAGTACGAGCGAGTGCCAGGATTGTCTGTCGGAAGCCGTCGGACAGCTCAGGTCCGGGTGCGGGACCAGCGCGTGGGGCGACATGTTCTTGGCTAAGTGCTACGTCCGCTACTCGGAAGGTGGATATCACCCGAACGGCGGTCATGATGATCATGATGACGACGACGACGATGAGCTTGAGAAGACACTTGCAATACTCATTGGAATTATAGCAGCAGTTGCACTACTCGTCGTATTTCTTTCCTACTTCCGAAAAAAAATTTATGGCGATGAAAAAGGTGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0030054 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

289

Amino Acids

30.7

Weight (kDa)

4.71

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 31 - 128 1.2e-18 Salt stress response/antifungal
Stress-antifung PF01657 160 - 232 2.1e-10 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01660 AT2G01660 AT2G01660
fragaria_vesca FvH4_4g33800 FvH4_4g33800 FvH4_4g33800
malus_domestica MD04G1025700.v1.1 MD13G1029600.v1.1 MD16G1020400.v1.1
prunus_persica Prupe.1G331900_v2.0.a1
pyrus_communis pycom16g01830
rosa_chinensis RchiOBHm_Chr4g0443311
rosa_laevigata RLG00000005939
rosa_multiflora Rmu_ssc0000122.1_g000006
rosa_roxburghii Rroxscaffold_5G00383890
rosa_rugosa Rorug04G0345500
rosa_samantha Rh4AG400400 Rh4BG412000 Rh4CG427400 Rh4DG407400
rosa_wichuraiana Rw4G034540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 651
AccI GTMKAC 1 cut(s) 440
AccII CGCG 2 cut(s) 347, 650
AciI CCGC 9 cut(s) 63, 87, 216, 339, 472, 534, 639, 685, 717
AclI AACGTT 1 cut(s) 389
AcsI RAATTY 1 cut(s) 837
AfaI GTAC 3 cut(s) 377, 524, 583
AfiI CCNNNNNNNGG 6 cut(s) 443, 507, 639, 651, 691, 716
AflIII ACRYGT 1 cut(s) 660
AjnI CCWGG 3 cut(s) 156, 225, 594
AluBI AGCT 4 cut(s) 213, 355, 625, 754
AluI AGCT 4 cut(s) 213, 355, 625, 754
Ama87I CYCGRG 2 cut(s) 112, 356
AoxI GGCC 2 cut(s) 241, 518
ApeKI GCWGC 4 cut(s) 210, 352, 363, 791
ApoI RAATTY 1 cut(s) 837
AspLEI GCGC 3 cut(s) 347, 562, 650
AspS9I GGNCC 4 cut(s) 434, 498, 630, 642
AsuC2I CCSGG 5 cut(s) 113, 114, 204, 541, 634
AsuHPI GGTGA 4 cut(s) 142, 187, 316, 698
AvaI CYCGRG 2 cut(s) 112, 356
AvaII GGWCC 4 cut(s) 434, 498, 630, 642
BbsI GAAGAC 2 cut(s) 73, 767
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 4 cut(s) 222, 350, 364, 803
BccI CCATC 1 cut(s) 500
BceAI ACGGC 5 cut(s) 240, 464, 505, 599, 730
BciT130I CCWGG 3 cut(s) 158, 227, 596
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 5 cut(s) 113, 114, 204, 541, 634
BglI GCCNNNNNGGC 1 cut(s) 485
BisI GCNGC 6 cut(s) 88, 211, 353, 364, 535, 792
BlsI GCNGC 6 cut(s) 89, 212, 354, 365, 536, 793
Bme1390I CCNGG 8 cut(s) 113, 114, 158, 204, 227, 541, 596, 634
Bme18I GGWCC 4 cut(s) 434, 498, 630, 642
BmeT110I CYCGRG 2 cut(s) 112, 356
BmgT120I GGNCC 4 cut(s) 434, 498, 630, 642
BmiI GGNNCC 5 cut(s) 223, 319, 499, 538, 643
BmrFI CCNGG 8 cut(s) 113, 114, 158, 204, 227, 541, 596, 634
BmrI ACTGGG 2 cut(s) 280, 453
BmsI GCATC 1 cut(s) 466
BmuI ACTGGG 2 cut(s) 280, 453
BpiI GAAGAC 2 cut(s) 73, 767
Bpu10I CCTNAGC 2 cut(s) 266, 626
BpuEI CTTGAG 1 cut(s) 776
BpuMI CCSGG 5 cut(s) 113, 114, 204, 541, 634
BsaJI CCNNGG 6 cut(s) 112, 156, 202, 225, 256, 414
Bsc4I CCNNNNNNNGG 6 cut(s) 443, 507, 639, 651, 691, 716
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 286, 448
BseBI CCWGG 3 cut(s) 158, 227, 596
BseDI CCNNGG 6 cut(s) 112, 156, 202, 225, 256, 414
BseGI GGATG 2 cut(s) 56, 481
BseLI CCNNNNNNNGG 6 cut(s) 443, 507, 639, 651, 691, 716
BseMII CTCAG 3 cut(s) 180, 280, 640
BseNI ACTGG 2 cut(s) 286, 448
BseRI GAGGAG 2 cut(s) 17, 137
BseXI GCAGC 4 cut(s) 222, 350, 364, 803
BseYI CCCAGC 1 cut(s) 96
Bsh1236I CGCG 2 cut(s) 347, 650
BshFI GGCC 2 cut(s) 243, 520
BsiHKCI CYCGRG 2 cut(s) 112, 356
BsiSI CCGG 5 cut(s) 113, 204, 219, 540, 633
BslFI GGGAC 3 cut(s) 511, 523, 655
BslI CCNNNNNNNGG 6 cut(s) 443, 507, 639, 651, 691, 716
BsmFI GGGAC 3 cut(s) 511, 523, 655
BsnI GGCC 2 cut(s) 243, 520
BsoBI CYCGRG 2 cut(s) 112, 356
Bsp143I GATC 1 cut(s) 727
BspACI CCGC 9 cut(s) 63, 87, 216, 339, 472, 534, 639, 685, 717
BspANI GGCC 2 cut(s) 243, 520
BspCNI CTCAG 3 cut(s) 179, 279, 639
BspFNI CGCG 2 cut(s) 347, 650
BspHI TCATGA 2 cut(s) 721, 730
BspLI GGNNCC 5 cut(s) 223, 319, 499, 538, 643
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 2 cut(s) 286, 448
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 6 cut(s) 112, 156, 202, 225, 256, 414
BssMI GATC 1 cut(s) 727
Bst2UI CCWGG 3 cut(s) 158, 227, 596
Bst4CI ACNGT 2 cut(s) 415, 438
Bst6I CTCTTC 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 797
BstC8I GCNNGC 3 cut(s) 220, 298, 486
BstDEI CTNAG 5 cut(s) 166, 266, 394, 626, 672
BstDSI CCRYGG 2 cut(s) 256, 414
BstF5I GGATG 2 cut(s) 56, 481
BstFNI CGCG 2 cut(s) 347, 650
BstHHI GCGC 3 cut(s) 347, 562, 650
BstKTI GATC 1 cut(s) 730
BstMBI GATC 1 cut(s) 727
BstMWI GCNNNNNNNGC 9 cut(s) 219, 267, 288, 297, 485, 645, 654, 684, 797
BstNI CCWGG 3 cut(s) 158, 227, 596
BstNSI RCATGY 1 cut(s) 664
BstSCI CCNGG 8 cut(s) 111, 112, 156, 202, 225, 539, 594, 632
BstUI CGCG 2 cut(s) 347, 650
BstV1I GCAGC 4 cut(s) 222, 350, 364, 803
BstV2I GAAGAC 2 cut(s) 73, 767
BstXI CCANNNNNNTGG 1 cut(s) 257
BsuRI GGCC 2 cut(s) 243, 520
BtgI CCRYGG 2 cut(s) 256, 414
BtgZI GCGATG 2 cut(s) 519, 861
BtsCI GGATG 2 cut(s) 56, 481
BtsI GCAGTG 1 cut(s) 569
BtsIMutI CAGTG 2 cut(s) 293, 569
Cac8I GCNNGC 3 cut(s) 220, 298, 486
CciI TCATGA 2 cut(s) 721, 730
CfoI GCGC 3 cut(s) 347, 562, 650
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 4 cut(s) 434, 498, 630, 642
Cfr9I CCCGGG 1 cut(s) 112
CseI GACGC 1 cut(s) 280
Csp6I GTAC 3 cut(s) 376, 523, 582
CviAII CATG 4 cut(s) 172, 661, 722, 731
CviQI GTAC 3 cut(s) 376, 523, 582
DdeI CTNAG 5 cut(s) 166, 266, 394, 626, 672
DpnI GATC 1 cut(s) 729
DpnII GATC 1 cut(s) 727
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 205
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 4 cut(s) 434, 498, 630, 642
Eco88I CYCGRG 2 cut(s) 112, 356
EcoRII CCWGG 3 cut(s) 156, 225, 594
EcoRV GATATC 1 cut(s) 704
FaeI CATG 4 cut(s) 175, 664, 725, 734
FaiI YATR 8 cut(s) 173, 238, 240, 662, 723, 732, 788, 843
FaqI GGGAC 3 cut(s) 511, 523, 655
FatI CATG 4 cut(s) 171, 660, 721, 730
FauI CCCGC 2 cut(s) 465, 632
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 440
Fnu4HI GCNGC 6 cut(s) 88, 211, 353, 364, 535, 792
FokI GGATG 2 cut(s) 43, 488
Fsp4HI GCNGC 6 cut(s) 88, 211, 353, 364, 535, 792
GlaI GCGC 3 cut(s) 346, 561, 649
GluI GCNGC 6 cut(s) 88, 211, 353, 364, 535, 792
GsaI CCCAGC 1 cut(s) 100
HaeIII GGCC 2 cut(s) 243, 520
HapII CCGG 5 cut(s) 113, 204, 219, 540, 633
HgaI GACGC 1 cut(s) 280
HhaI GCGC 3 cut(s) 347, 562, 650
Hin1II CATG 4 cut(s) 175, 664, 725, 734
Hin6I GCGC 3 cut(s) 345, 560, 648
HinP1I GCGC 3 cut(s) 345, 560, 648
HincII GTYRAC 4 cut(s) 163, 311, 441, 465
HindII GTYRAC 4 cut(s) 163, 311, 441, 465
HinfI GANTC 6 cut(s) 128, 307, 332, 452, 458, 528
HpaII CCGG 5 cut(s) 113, 204, 219, 540, 633
HphI GGTGA 4 cut(s) 142, 187, 316, 698
Hpy166II GTNNAC 6 cut(s) 139, 163, 311, 434, 441, 465
Hpy188I TCNGA 6 cut(s) 317, 457, 610, 620, 694, 830
Hpy188III TCNNGA 2 cut(s) 722, 731
Hpy8I GTNNAC 6 cut(s) 139, 163, 311, 434, 441, 465
Hpy99I CGWCG 7 cut(s) 296, 442, 620, 743, 746, 749, 812
HpyAV CCTTC 4 cut(s) 49, 85, 208, 689
HpyCH4III ACNGT 2 cut(s) 415, 438
HpyCH4IV ACGT 2 cut(s) 389, 681
HpyCH4V TGCA 3 cut(s) 352, 770, 800
HpyF10VI GCNNNNNNNGC 9 cut(s) 219, 267, 288, 297, 485, 645, 654, 684, 797
HpyF3I CTNAG 5 cut(s) 166, 266, 394, 626, 672
HpySE526I ACGT 2 cut(s) 389, 681
Hsp92II CATG 4 cut(s) 175, 664, 725, 734
HspAI GCGC 3 cut(s) 345, 560, 648
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 1 cut(s) 727
LmnI GCTCC 5 cut(s) 150, 218, 227, 287, 542
Lsp1109I GCAGC 4 cut(s) 222, 350, 364, 803
LweI GCATC 1 cut(s) 466
MaeII ACGT 2 cut(s) 389, 681
MalI GATC 1 cut(s) 729
MboI GATC 1 cut(s) 727
MboII GAAGA 3 cut(s) 73, 238, 772
MluCI AATT 2 cut(s) 783, 837
MlyI GAGTC 5 cut(s) 137, 316, 326, 446, 537
MmeI TCCRAC 2 cut(s) 588, 598
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 534
MspI CCGG 5 cut(s) 113, 204, 219, 540, 633
MspR9I CCNGG 8 cut(s) 113, 114, 158, 204, 227, 541, 596, 634
MvaI CCWGG 3 cut(s) 158, 227, 596
MvnI CGCG 2 cut(s) 347, 650
MwoI GCNNNNNNNGC 9 cut(s) 219, 267, 288, 297, 485, 645, 654, 684, 797
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 5 cut(s) 113, 114, 204, 541, 634
NdeII GATC 1 cut(s) 727
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 4 cut(s) 175, 664, 725, 734
NlaIV GGNNCC 5 cut(s) 223, 319, 499, 538, 643
NspI RCATGY 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 356
PagI TCATGA 2 cut(s) 721, 730
PciI ACATGT 1 cut(s) 660
PcsI WCGNNNNNNNCGW 1 cut(s) 744
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 458
PflMI CCANNNNNTGG 1 cut(s) 651
PfoI TCCNGGA 1 cut(s) 539
PkrI GCNGC 6 cut(s) 89, 212, 354, 365, 536, 793
PleI GAGTC 5 cut(s) 136, 315, 326, 446, 536
PpsI GAGTC 5 cut(s) 136, 315, 326, 446, 536
PscI ACATGT 1 cut(s) 660
Psp1406I AACGTT 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 156, 225, 594
PspFI CCCAGC 1 cut(s) 96
PspGI CCWGG 3 cut(s) 156, 225, 594
PspN4I GGNNCC 5 cut(s) 223, 319, 499, 538, 643
PspPI GGNCC 4 cut(s) 434, 498, 630, 642
PspXI VCTCGAGB 1 cut(s) 356
RsaI GTAC 3 cut(s) 377, 524, 583
RsaNI GTAC 3 cut(s) 376, 523, 582
SalI GTCGAC 1 cut(s) 439
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 6 cut(s) 88, 211, 353, 364, 535, 792
Sau3AI GATC 1 cut(s) 727
Sau96I GGNCC 4 cut(s) 434, 498, 630, 642
SchI GAGTC 5 cut(s) 137, 316, 326, 446, 537
ScrFI CCNGG 8 cut(s) 113, 114, 158, 204, 227, 541, 596, 634
SfaNI GCATC 1 cut(s) 466
Sfr274I CTCGAG 1 cut(s) 356
SinI GGWCC 4 cut(s) 434, 498, 630, 642
SlaI CTCGAG 1 cut(s) 356
SmaI CCCGGG 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 356, 755
SmoI CTYRAG 2 cut(s) 356, 755
Sse9I AATT 2 cut(s) 783, 837
SsiI CCGC 9 cut(s) 63, 87, 216, 339, 472, 534, 639, 685, 717
StyD4I CCNGG 8 cut(s) 111, 112, 156, 202, 225, 539, 594, 632
TaaI ACNGT 2 cut(s) 415, 438
TaiI ACGT 2 cut(s) 392, 684
TaqI TCGA 4 cut(s) 131, 330, 357, 440
TasI AATT 2 cut(s) 783, 837
TauI GCSGC 2 cut(s) 90, 537
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 2 cut(s) 293, 569
TseI GCWGC 4 cut(s) 210, 352, 363, 791
TspDTI ATGAA 1 cut(s) 864
TspMI CCCGGG 1 cut(s) 112
TspRI CASTG 2 cut(s) 293, 569
Van91I CCANNNNNTGG 1 cut(s) 651
VpaK11BI GGWCC 4 cut(s) 434, 498, 630, 642
XapI RAATTY 1 cut(s) 837
XceI RCATGY 1 cut(s) 664
XhoI CTCGAG 1 cut(s) 356
XmaI CCCGGG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.