Rh4BG412000

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009865
Physical Location & Seq
Forward (+)
56078346 .. 56080923
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG412000.1

Sequence Viewer

Length: 894 bp
ATGTCTCTAACCGGAAATATCACTGCAACTTTCTTCGTCTTCTTTCTCGTCAATCTCTCCTTCTTCACATCCCCGTCGTCCTCCGCAACCGACACGTTCATCTACGGCGGTTGCTCCCAGCTCAAGTTTTCCCCGGGCTCGCCGTACGAGATGAAGCTCAACTCCATATTGGCCTCCGTGGTCAACTCGGCCATGTTCACCACTTACAACAACTTCACGGTCCCCGGTTCGTCAGGCACTGCAGACACGCTCTACGGCGTGTTCCAGTGCCGCGGCGACTTGAGCGGCAATGACTGCGGGCAGTGCGTGACGCGCGCAGTCAGCCAGCTAGGCACGCTCTGCCTGGACGCGTGCGGCGGCGCGTTGCAGCTGGACGGGTGCTTCGTCAAGTACGACAACGCCACGTTCTTAGGGGTGGAGGACAAGACGGTGGTGATGAAGAAGTGTGGGCCGTCGATTGGGTATGACTCGGAGGGGTTGACCAGGCGGGATGCTGTGCTGGCTTTTCTCGGGACCAGTGATGGGGCCTACCGGCCGTTCCGGGTGAGTGGCGCCGGAAATGTCCAGGGTGTGGCGCAGTGCGTCGGGGACTTGAGTCCGAGCGAGTGCCAGGATTGTCTGTCCGACGCCGTAGGGCAGCTGAGGGCGGGGTGTGGGACCAGCACGTGGGGGGACTTGTTCTTGGCCAAGTGCTACGCGCGCTACTCGGAAGGTGGATATCACTCGCAGGGCCATGATCACAATGACGACGACGACGATGATGATGATCTTGATAGGACGCTTGCAATACTGATTGGAATCATAGCAGCAGTTGCACTGCTCGTCGTATTTCTTTCTTACTTCAGAAAATATTTGTGTGGAGATGAGTGTGGAGATGAAAAAGGTGGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0030054 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

297

Amino Acids

31.51

Weight (kDa)

4.46

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 38 - 133 4.2e-18 Salt stress response/antifungal
Stress-antifung PF01657 182 - 237 1.2e-09 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01660 AT2G01660 AT2G01660
fragaria_vesca FvH4_4g33800 FvH4_4g33800 FvH4_4g33800
malus_domestica MD04G1025700.v1.1 MD13G1029600.v1.1 MD16G1020400.v1.1
prunus_persica Prupe.1G331900_v2.0.a1
pyrus_communis pycom16g01830
rosa_chinensis RchiOBHm_Chr4g0443311
rosa_laevigata RLG00000005939
rosa_multiflora Rmu_ssc0000122.1_g000006
rosa_roxburghii Rroxscaffold_5G00383890
rosa_rugosa Rorug04G0345500
rosa_samantha Rh4AG400400 Rh4BG412000 Rh4CG427400 Rh4DG407400
rosa_wichuraiana Rw4G034540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 551
AccB7I CCANNNNNTGG 2 cut(s) 571, 666
AccBSI CCGCTC 1 cut(s) 285
AccII CGCG 7 cut(s) 273, 313, 315, 350, 362, 698, 700
AcoI YGGCCR 3 cut(s) 189, 533, 684
AcuI CTGAAG 1 cut(s) 826
AcvI CACGTG 1 cut(s) 666
AcyI GRCGYC 2 cut(s) 552, 627
AfaI GTAC 2 cut(s) 146, 392
AfiI CCNNNNNNNGG 4 cut(s) 458, 522, 571, 666
AflIII ACRYGT 2 cut(s) 93, 348
AjnI CCWGG 4 cut(s) 342, 482, 564, 609
AluBI AGCT 5 cut(s) 121, 157, 328, 370, 640
AluI AGCT 5 cut(s) 121, 157, 328, 370, 640
Alw26I GTCTC 1 cut(s) 9
AlwNI CAGNNNCTG 1 cut(s) 239
Ama87I CYCGRG 2 cut(s) 133, 509
AoxI GGCC 7 cut(s) 171, 189, 449, 525, 533, 684, 730
ApeKI GCWGC 3 cut(s) 367, 637, 806
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AspLEI GCGC 7 cut(s) 315, 317, 362, 554, 577, 700, 702
AspS9I GGNCC 6 cut(s) 220, 449, 513, 525, 657, 730
AsuC2I CCSGG 4 cut(s) 134, 135, 225, 542
AsuHPI GGTGA 3 cut(s) 190, 445, 556
AvaI CYCGRG 2 cut(s) 133, 509
AvaII GGWCC 3 cut(s) 220, 513, 657
BalI TGGCCA 1 cut(s) 686
BanI GGYRCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 140
BbrPI CACGTG 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 31
BbvCI CCTCAGC 1 cut(s) 641
BbvI GCAGC 3 cut(s) 379, 649, 818
BccI CCATC 1 cut(s) 515
BceAI ACGGC 6 cut(s) 121, 127, 271, 436, 520, 614
BciT130I CCWGG 4 cut(s) 344, 484, 566, 611
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 4 cut(s) 134, 135, 225, 542
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 1 cut(s) 555
BglI GCCNNNNNGGC 1 cut(s) 330
BisI GCNGC 8 cut(s) 271, 274, 286, 355, 358, 368, 638, 807
BlsI GCNGC 8 cut(s) 272, 275, 287, 356, 359, 369, 639, 808
Bme1390I CCNGG 8 cut(s) 134, 135, 225, 344, 484, 542, 566, 611
Bme18I GGWCC 3 cut(s) 220, 513, 657
BmeT110I CYCGRG 2 cut(s) 133, 509
BmgT120I GGNCC 6 cut(s) 220, 449, 513, 525, 657, 730
BmiI GGNNCC 5 cut(s) 222, 514, 526, 553, 658
BmrFI CCNGG 8 cut(s) 134, 135, 225, 344, 484, 542, 566, 611
BmsI GCATC 1 cut(s) 481
BoxI GACNNNNGTC 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 641
BpuEI CTTGAG 3 cut(s) 107, 301, 613
BpuMI CCSGG 4 cut(s) 134, 135, 225, 542
BsaAI YACGTR 1 cut(s) 666
BsaBI GATNNNNATC 2 cut(s) 765, 797
BsaHI GRCGYC 2 cut(s) 552, 627
BsaJI CCNNGG 6 cut(s) 132, 133, 177, 223, 271, 565
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 458, 522, 571, 666
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 2 cut(s) 265, 516
Bse3DI GCAATG 1 cut(s) 295
Bse8I GATNNNNATC 2 cut(s) 765, 797
BseBI CCWGG 4 cut(s) 344, 484, 566, 611
BseDI CCNNGG 6 cut(s) 132, 133, 177, 223, 271, 565
BseGI GGATG 2 cut(s) 68, 496
BseJI GATNNNNATC 2 cut(s) 765, 797
BseLI CCNNNNNNNGG 4 cut(s) 458, 522, 571, 666
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 632
BseNI ACTGG 2 cut(s) 265, 516
BsePI GCGCGC 2 cut(s) 313, 698
BseX3I CGGCCG 1 cut(s) 533
BseXI GCAGC 3 cut(s) 379, 649, 818
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 7 cut(s) 273, 313, 315, 350, 362, 698, 700
Bsh1285I CGRYCG 1 cut(s) 536
BshFI GGCC 7 cut(s) 173, 191, 451, 527, 535, 686, 732
BshNI GGYRCC 1 cut(s) 551
BsiEI CGRYCG 1 cut(s) 536
BsiHKCI CYCGRG 2 cut(s) 133, 509
BsiSI CCGG 6 cut(s) 12, 134, 225, 532, 541, 555
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 5 cut(s) 206, 526, 602, 670, 686
BslI CCNNNNNNNGG 4 cut(s) 458, 522, 571, 666
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 5 cut(s) 206, 526, 602, 670, 686
BsnI GGCC 7 cut(s) 173, 191, 451, 527, 535, 686, 732
BsoBI CYCGRG 2 cut(s) 133, 509
Bsp1286I GDGCHC 1 cut(s) 140
Bsp143I GATC 2 cut(s) 736, 766
BspANI GGCC 7 cut(s) 173, 191, 451, 527, 535, 686, 732
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 7 cut(s) 273, 313, 315, 350, 362, 698, 700
BspLI GGNNCC 5 cut(s) 222, 514, 526, 553, 658
BspMAI CTGCAG 1 cut(s) 244
BspT107I GGYRCC 1 cut(s) 551
BsrBI CCGCTC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 2 cut(s) 265, 516
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 6 cut(s) 132, 133, 177, 223, 271, 565
BssHII GCGCGC 2 cut(s) 313, 698
BssMI GATC 2 cut(s) 736, 766
BssNI GRCGYC 2 cut(s) 552, 627
Bst2UI CCWGG 4 cut(s) 344, 484, 566, 611
Bst4CI ACNGT 2 cut(s) 220, 430
BstACI GRCGYC 2 cut(s) 552, 627
BstAPI GCANNNNNTGC 3 cut(s) 294, 339, 812
BstBAI YACGTR 1 cut(s) 666
BstC8I GCNNGC 9 cut(s) 140, 299, 315, 326, 335, 352, 501, 700, 783
BstDEI CTNAG 2 cut(s) 409, 641
BstDSI CCRYGG 2 cut(s) 177, 271
BstF5I GGATG 2 cut(s) 68, 496
BstFNI CGCG 7 cut(s) 273, 313, 315, 350, 362, 698, 700
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 7 cut(s) 315, 317, 362, 554, 577, 700, 702
BstKTI GATC 2 cut(s) 739, 769
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 736, 766
BstMCI CGRYCG 1 cut(s) 536
BstNI CCWGG 4 cut(s) 344, 484, 566, 611
BstPAI GACNNNNGTC 1 cut(s) 594
BstSCI CCNGG 8 cut(s) 132, 133, 223, 342, 482, 540, 564, 609
BstSFI CTRYAG 1 cut(s) 240
BstUI CGCG 7 cut(s) 273, 313, 315, 350, 362, 698, 700
BstV1I GCAGC 3 cut(s) 379, 649, 818
BstV2I GAAGAC 1 cut(s) 31
BstZI CGGCCG 1 cut(s) 533
BsuRI GGCC 7 cut(s) 173, 191, 451, 527, 535, 686, 732
BtgI CCRYGG 2 cut(s) 177, 271
BtsCI GGATG 2 cut(s) 68, 496
BtsI GCAGTG 5 cut(s) 21, 237, 308, 584, 815
BtsIMutI CAGTG 7 cut(s) 21, 237, 272, 308, 523, 584, 815
Cac8I GCNNGC 9 cut(s) 140, 299, 315, 326, 335, 352, 501, 700, 783
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 7 cut(s) 315, 317, 362, 554, 577, 700, 702
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 6 cut(s) 220, 449, 513, 525, 657, 730
Cfr42I CCGCGG 1 cut(s) 274
Cfr9I CCCGGG 1 cut(s) 133
CseI GACGC 5 cut(s) 319, 356, 571, 635, 787
Csp6I GTAC 2 cut(s) 145, 391
CviAII CATG 2 cut(s) 193, 734
CviQI GTAC 2 cut(s) 145, 391
DdeI CTNAG 2 cut(s) 409, 641
DinI GGCGCC 1 cut(s) 553
DpnI GATC 2 cut(s) 738, 768
DpnII GATC 2 cut(s) 736, 766
EaeI YGGCCR 3 cut(s) 189, 533, 684
EagI CGGCCG 1 cut(s) 533
EclXI CGGCCG 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 719
Eco47I GGWCC 3 cut(s) 220, 513, 657
Eco52I CGGCCG 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 826
Eco72I CACGTG 1 cut(s) 666
Eco88I CYCGRG 2 cut(s) 133, 509
EcoO109I RGGNCCY 1 cut(s) 525
EcoRII CCWGG 4 cut(s) 342, 482, 564, 609
EcoRV GATATC 1 cut(s) 719
EcoT38I GRGCYC 1 cut(s) 140
EgeI GGCGCC 1 cut(s) 553
EheI GGCGCC 1 cut(s) 553
FaeI CATG 2 cut(s) 196, 737
FaiI YATR 5 cut(s) 167, 194, 465, 735, 803
FaqI GGGAC 5 cut(s) 206, 526, 602, 670, 686
FatI CATG 2 cut(s) 192, 733
FauI CCCGC 3 cut(s) 290, 480, 640
FbaI TGATCA 1 cut(s) 736
Fnu4HI GCNGC 8 cut(s) 271, 274, 286, 355, 358, 368, 638, 807
FokI GGATG 2 cut(s) 55, 503
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 8 cut(s) 271, 274, 286, 355, 358, 368, 638, 807
FspBI CTAG 1 cut(s) 329
GlaI GCGC 7 cut(s) 314, 316, 361, 553, 576, 699, 701
GluI GCNGC 8 cut(s) 271, 274, 286, 355, 358, 368, 638, 807
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 555
HaeIII GGCC 7 cut(s) 173, 191, 451, 527, 535, 686, 732
HapII CCGG 6 cut(s) 12, 134, 225, 532, 541, 555
HgaI GACGC 5 cut(s) 319, 356, 571, 635, 787
HhaI GCGC 7 cut(s) 315, 317, 362, 554, 577, 700, 702
Hin1I GRCGYC 2 cut(s) 552, 627
Hin1II CATG 2 cut(s) 196, 737
Hin6I GCGC 7 cut(s) 313, 315, 360, 552, 575, 698, 700
HinP1I GCGC 7 cut(s) 313, 315, 360, 552, 575, 698, 700
HincII GTYRAC 2 cut(s) 184, 480
HindII GTYRAC 2 cut(s) 184, 480
HinfI GANTC 3 cut(s) 467, 595, 798
HpaII CCGG 6 cut(s) 12, 134, 225, 532, 541, 555
HphI GGTGA 3 cut(s) 190, 445, 556
Hpy166II GTNNAC 3 cut(s) 184, 198, 480
Hpy188I TCNGA 5 cut(s) 472, 600, 625, 709, 845
Hpy188III TCNNGA 2 cut(s) 511, 770
Hpy8I GTNNAC 3 cut(s) 184, 198, 480
Hpy99I CGWCG 8 cut(s) 79, 457, 587, 629, 752, 755, 758, 827
HpyAV CCTTC 2 cut(s) 70, 704
HpyCH4III ACNGT 2 cut(s) 220, 430
HpyCH4IV ACGT 3 cut(s) 95, 404, 665
HpyCH4V TGCA 5 cut(s) 26, 242, 367, 785, 815
HpyF3I CTNAG 2 cut(s) 409, 641
HpySE526I ACGT 3 cut(s) 95, 404, 665
Hsp92I GRCGYC 2 cut(s) 552, 627
Hsp92II CATG 2 cut(s) 196, 737
HspAI GCGC 7 cut(s) 313, 315, 360, 552, 575, 698, 700
KasI GGCGCC 1 cut(s) 551
Ksp22I TGATCA 1 cut(s) 736
KspI CCGCGG 1 cut(s) 274
Kzo9I GATC 2 cut(s) 736, 766
LmnI GCTCC 1 cut(s) 119
Lsp1109I GCAGC 3 cut(s) 379, 649, 818
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 329
MaeII ACGT 3 cut(s) 95, 404, 665
MaeIII GTNAC 1 cut(s) 307
MalI GATC 2 cut(s) 738, 768
MbiI CCGCTC 1 cut(s) 285
MboI GATC 2 cut(s) 736, 766
MboII GAAGA 4 cut(s) 25, 31, 55, 451
MhlI GDGCHC 1 cut(s) 140
MlsI TGGCCA 1 cut(s) 686
MluI ACGCGT 1 cut(s) 348
MluNI TGGCCA 1 cut(s) 686
Mly113I GGCGCC 1 cut(s) 552
MlyI GAGTC 2 cut(s) 461, 604
MmeI TCCRAC 1 cut(s) 648
MnlI CCTC 5 cut(s) 91, 184, 412, 466, 636
Mox20I TGGCCA 1 cut(s) 686
MscI TGGCCA 1 cut(s) 686
Msp20I TGGCCA 1 cut(s) 686
MspA1I CMGCKG 3 cut(s) 273, 370, 640
MspI CCGG 6 cut(s) 12, 134, 225, 532, 541, 555
MspR9I CCNGG 8 cut(s) 134, 135, 225, 344, 484, 542, 566, 611
MvaI CCWGG 4 cut(s) 344, 484, 566, 611
MvnI CGCG 7 cut(s) 273, 313, 315, 350, 362, 698, 700
NarI GGCGCC 1 cut(s) 552
NciI CCSGG 4 cut(s) 134, 135, 225, 542
NdeII GATC 2 cut(s) 736, 766
NlaIII CATG 2 cut(s) 196, 737
NlaIV GGNNCC 5 cut(s) 222, 514, 526, 553, 658
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 1 cut(s) 307
PauI GCGCGC 2 cut(s) 313, 698
PcsI WCGNNNNNNNCGW 3 cut(s) 381, 390, 753
PfeI GAWTC 1 cut(s) 798
Pfl23II CGTACG 1 cut(s) 144
PflMI CCANNNNNTGG 2 cut(s) 571, 666
PkrI GCNGC 8 cut(s) 272, 275, 287, 356, 359, 369, 639, 808
PleI GAGTC 2 cut(s) 461, 603
PluTI GGCGCC 1 cut(s) 555
PmaCI CACGTG 1 cut(s) 666
PmlI CACGTG 1 cut(s) 666
PpsI GAGTC 2 cut(s) 461, 603
Ppu21I YACGTR 1 cut(s) 666
PshAI GACNNNNGTC 1 cut(s) 594
Psp6I CCWGG 4 cut(s) 342, 482, 564, 609
PspCI CACGTG 1 cut(s) 666
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 4 cut(s) 342, 482, 564, 609
PspLI CGTACG 1 cut(s) 144
PspN4I GGNNCC 5 cut(s) 222, 514, 526, 553, 658
PspPI GGNCC 6 cut(s) 220, 449, 513, 525, 657, 730
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 239
PteI GCGCGC 2 cut(s) 313, 698
PvuII CAGCTG 2 cut(s) 370, 640
RsaI GTAC 2 cut(s) 146, 392
RsaNI GTAC 2 cut(s) 145, 391
SacII CCGCGG 1 cut(s) 274
SatI GCNGC 8 cut(s) 271, 274, 286, 355, 358, 368, 638, 807
Sau3AI GATC 2 cut(s) 736, 766
Sau96I GGNCC 6 cut(s) 220, 449, 513, 525, 657, 730
SchI GAGTC 2 cut(s) 461, 604
ScrFI CCNGG 8 cut(s) 134, 135, 225, 344, 484, 542, 566, 611
SduI GDGCHC 1 cut(s) 140
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 240
SfoI GGCGCC 1 cut(s) 553
Sfr303I CCGCGG 1 cut(s) 274
SgrBI CCGCGG 1 cut(s) 274
SinI GGWCC 3 cut(s) 220, 513, 657
SmaI CCCGGG 1 cut(s) 135
SmlI CTYRAG 3 cut(s) 122, 280, 592
SmoI CTYRAG 3 cut(s) 122, 280, 592
SspDI GGCGCC 1 cut(s) 551
SspI AATATT 1 cut(s) 851
SspMI CTAG 1 cut(s) 329
StyD4I CCNGG 8 cut(s) 132, 133, 223, 342, 482, 540, 564, 609
TaaI ACNGT 2 cut(s) 220, 430
TaiI ACGT 3 cut(s) 98, 407, 668
TaqI TCGA 1 cut(s) 455
TauI GCSGC 5 cut(s) 273, 276, 288, 357, 360
TfiI GAWTC 1 cut(s) 798
TscAI CASTG 7 cut(s) 28, 244, 272, 308, 523, 584, 822
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 3 cut(s) 367, 637, 806
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 4 cut(s) 88, 167, 452, 891
TspGWI ACGGA 1 cut(s) 166
TspMI CCCGGG 1 cut(s) 133
TspRI CASTG 7 cut(s) 28, 244, 272, 308, 523, 584, 822
Van91I CCANNNNNTGG 2 cut(s) 571, 666
VpaK11BI GGWCC 3 cut(s) 220, 513, 657
XmaI CCCGGG 1 cut(s) 133
XspI CTAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.