Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001564

Physical Location & Seq

Genomic Coordinates Reverse (-)

3896309 .. 3899298

Gene Structure 2990 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1055800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGATCGTTTTGGAATCGAATTTTCTACTCATTCCATCATTGGCTGCTTTGGAACTTGGCAACGTTCGGGAAGGGGAGAAGATCTCTTCTACTGCCGCCTCTCTTGCCGGCCTCCTAACTGTCACTTCCATTTGCTTTATGTCTCGGAAGCTGGACAGCCCAGTACAGACCCAGATGGCAGTGGCACTATTTAGCAATCCGCTCAGTGGGGAGTACCATGGAAGCAACAGAATGGAAAGGAAGCAGCACACTGGTTGGAGGCGCGTTTTTGTGCAAACTGAAAGTGGATGTGTCTTGGGGATGGATTTGGATCGCAGTGATAATGTCCATACTGTGAAGAGAAGATTGCAGATTGCTCTCAATGTCCCAACTGATGGAAGCTCGTTGACATTTGGGGATGTGGTGTTGAAGAACGATCTGAGTGCAGTTAGGAATGACTCCCCTCTTCTTCTCACAAGGAACCTTATGCATAGAAGTTCATCAACTCCATGTCTCTCGCCTACTGGAAAGGATCTGCAGCAGAGAGATCGGAGTGGTCCTATAGAGATATTAGGGAACTCGGTTCGGTTTACTAGGACAAGACCAGTGGTCAAGGATATGGTGAAGGCCATCAAGATGGGGGTTGATCCAATTCCTGTTCATAGTGGGCTTGGAGGTGCATACTATTTCAGGAACAGCAGAGGTGAGTGTGTTTCAATAGTGAAGCCAACTGATGAAGAACCTTTTGCGCCTAATAACCCAAAAGGTTTTGTCGGTAAAGCTCTTGGGCAACCAGGTTTGAAGCGCTCGGTGCGAGTTGGGGAGACTGGATTCCGAGAAGTCGCAGCTTTCCTGCTTGACAAAGATCACTTTGCCAATGTGCCTCCCACTGCCTTGGTGAAGATCACTCACTCTATCTTTAACATAAACGATGGGGTGAACGGGAACAAGACTCCCCAGAAGAAGCTGGTTAGCAAGATTGCATCTTGCCAACAATTCATACAACATGATTTTGATGCCAGTGATCATGGGACATCCAGCTTCCCCGTAACTTCTGTGCATCGTATAGGGATATTAGACATTAGGATTCTTAACACTGACAGGCATGCAGGGAACCTTTTAGTTAGGAAACTGGACGGTGTCGGGATGTTTGGTCAAGTGGAGCTCATTCCAATTGACCATGGCCTTTGTTTGCCAGAAACTTTGGAGGACCCCTACTTTGAGTGGATTCATTGGCCTCAGGCTTCTATTCCATTCTCAGATGATGAGCTTGAGTACATAGAAAAGCTTGATCCGCTTGAGGATTGTGAGATGCTGCGAAGGGAGCTTCCCATGATTCGAGAGGCTTGTCTCAGGGTTTTGATTCTTTGCACCATTTTCCTGAAGGAGTCTGCTGCATATGGTCTCTGTCTTGCTGAAATAGGTGAGATGATGACTAGGGAATTTCGCAGTGGCGAGGAGGAACCTAGTGAACTGGAGGTAATTTGTATGGAGGCAAGGACGATGTTAGCAGAGAGGGTGGAGGTGCTGTCCCCCAAGGCTGATTCGGGAGATCAGGAGTTCCTATTTGACATAGATTGTGAGGAAGCAGAGGAAGACTGCACCTTGAAATCTGCAGCAGATGATTATCTGACCAGGGCATCATCTTTTCCATTTGCAAATGGGAGTGGGCATGGCCGCTCGCTTCTCTCTAAACTTGAAGAAAGCATCGAGGAGGAAGAGGACAGTGAAGTGGAAGAGCAAGGGGCATTAGCAACTCTGCCAACTCTGGAAAAGGTCCCAAGCGTTTCAAAGCTTTCTATGTCACTGAAGAACACAGTTTTGGTGGGAGAGAAGAACCACCTGAAGCATATGGGTTCCAAACCAGGAAACGGATACATGGTGAATACATCATCGGGGCACAGGAGTGCAAACGAGCAGCTTCCTGCAAGCATGTGCTTTGTGAAGCTGGCTGACATGAGCGAGGATGCCTGGACCTTGTTTCTGGAGAAGTTTCAAGATATGCTGTACCCGGCATTTGCCAAACGCAAATCTGCTACCCTAGGTCAGAGGCAGAGACAGAGGCTTGGCACGTCGTGCCAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0030258 GO:0036211 GO:0043170 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0046486 GO:0046488 GO:0046777 GO:0046834 GO:0046854 GO:0052742 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PI3_PI4_kinase

Protein Analysis

689

Amino Acids

76.19

Weight (kDa)

5.53

Isoelectric Point (pI)

49.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI3_PI4_kinase	PF00454	218 - 466	4.7e-46	Phosphatidylinositol 3- and 4-kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13640 AT1G13640 AT1G26270 AT2G03890 AT2G03890
fragaria_vesca	FvH4_4g29190 FvH4_4g29190 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200
malus_domestica	MD13G1055800.v1.1 MD16G1054900.v1.1 MD17G1066800.v1.1
prunus_persica	Prupe.1G295100_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1
pyrus_communis	pycom13g04940 pycom16g04780 pycom17g06720
rosa_chinensis	RchiOBHm_Chr2g0164641 RchiOBHm_Chr4g0437261
rosa_laevigata	RLG00000006453 RLG00000021473
rosa_multiflora	Rmu_sc0004339.1_g000020 Rmu_ssc0000076.1_g000009
rosa_roxburghii	Rroxscaffold_2G00086410 Rroxscaffold_5G00378380
rosa_rugosa	Rorug02G0509500 Rorug04G0298800
rosa_samantha	Rh2AG577400 Rh2BG588600 Rh2CG559400 Rh2DG599200 Rh4AG353700 Rh4BG363500 Rh4CG377700 Rh4DG357600
rosa_wichuraiana	Rw2G047950 Rw4G031030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	374
AccBSI	CCGCTC	2 cut(s)	202, 1659
AccII	CGCG	1 cut(s)	264
AciI	CCGC	4 cut(s)	96, 200, 1274, 1657
AclI	AACGTT	1 cut(s)	63
AclWI	GGATC	4 cut(s)	318, 519, 620, 1265
AcoI	YGGCCR	1 cut(s)	1654
AcsI	RAATTY	2 cut(s)	19, 1421
AcuI	CTGAAG	3 cut(s)	1382, 1808, 1844
AdeI	CACNNNGTG	1 cut(s)	2055
AfaI	GTAC	4 cut(s)	165, 215, 1256, 1988
AfeI	AGCGCT	1 cut(s)	785
AfiI	CCNNNNNNNGG	3 cut(s)	206, 374, 1850
AgsI	TTSAA	7 cut(s)	409, 696, 781, 1588, 1679, 1770, 1976
AhdI	GACNNNNNGTC	1 cut(s)	587
AjiI	CACGTC	1 cut(s)	2052
AjnI	CCWGG	4 cut(s)	772, 1613, 1843, 1949
AjuI	GAANNNNNNNTTGG	2 cut(s)	1784, 1816
AleI	CACNNNNGTG	1 cut(s)	1884
Alw21I	GWGCWC	1 cut(s)	1146
Alw26I	GTCTC	6 cut(s)	147, 497, 797, 1334, 1388, 2029
AlwI	GGATC	4 cut(s)	318, 519, 620, 1265
Aor51HI	AGCGCT	1 cut(s)	785
AoxI	GGCC	5 cut(s)	109, 606, 1162, 1214, 1654
ApeKI	GCWGC	8 cut(s)	44, 244, 517, 824, 1294, 1373, 1595, 1897
ApoI	RAATTY	2 cut(s)	19, 1421
ArsI	GACNNNNNNTTYG	2 cut(s)	1181, 1213
AspA2I	CCTAGG	1 cut(s)	2020
AspLEI	GCGC	3 cut(s)	264, 730, 786
AspS9I	GGNCC	4 cut(s)	536, 1189, 1756, 1953
AsuC2I	CCSGG	1 cut(s)	1991
AsuHPI	GGTGA	6 cut(s)	613, 695, 889, 928, 1415, 1873
AvaII	GGWCC	4 cut(s)	536, 1189, 1756, 1953
AvrII	CCTAGG	1 cut(s)	2020
AxyI	CCTNAGG	1 cut(s)	1218
BaeGI	GKGCMC	1 cut(s)	1881
BanII	GRGCYC	1 cut(s)	1146
BbsI	GAAGAC	1 cut(s)	1581
Bbv12I	GWGCWC	1 cut(s)	1146
BbvI	GCAGC	8 cut(s)	31, 256, 529, 836, 1281, 1360, 1607, 1909
BccI	CCATC	7 cut(s)	43, 169, 295, 368, 610, 617, 905
BcgI	CGANNNNNNTGC	4 cut(s)	404, 438, 509, 543
BciT130I	CCWGG	4 cut(s)	774, 1615, 1845, 1951
BciVI	GTATCC	1 cut(s)	1847
BclI	TGATCA	1 cut(s)	1003
BcnI	CCSGG	1 cut(s)	1991
BcoDI	GTCTC	6 cut(s)	147, 497, 797, 1334, 1388, 2029
BfaI	CTAG	4 cut(s)	573, 1416, 1446, 2021
BfmI	CTRYAG	3 cut(s)	515, 540, 1593
BfoI	RGCGCY	1 cut(s)	787
BfuI	GTATCC	1 cut(s)	1847
BglII	AGATCT	1 cut(s)	81
BlnI	CCTAGG	1 cut(s)	2020
Bme1390I	CCNGG	5 cut(s)	774, 1615, 1845, 1951, 1991
Bme18I	GGWCC	4 cut(s)	536, 1189, 1756, 1953
BmeRI	GACNNNNNGTC	1 cut(s)	587
BmgBI	CACGTC	1 cut(s)	2052
BmgT120I	GGNCC	4 cut(s)	536, 1189, 1756, 1953
BmiI	GGNNCC	6 cut(s)	461, 1094, 1191, 1443, 1758, 1837
BmrFI	CCNGG	5 cut(s)	774, 1615, 1845, 1951, 1991
BmrI	ACTGGG	1 cut(s)	155
BmsI	GCATC	7 cut(s)	971, 985, 1048, 1281, 1628, 1695, 1936
BmuI	ACTGGG	1 cut(s)	155
BpiI	GAAGAC	1 cut(s)	1581
BplI	GAGNNNNNCTC	2 cut(s)	68, 100
BpmI	CTGGAG	2 cut(s)	1475, 1985
BpuEI	CTTGAG	2 cut(s)	1271, 1298
BpuMI	CCSGG	1 cut(s)	1991
BsaBI	GATNNNNATC	2 cut(s)	309, 1605
BsaI	GGTCTC	1 cut(s)	1388
BsaJI	CCNNGG	6 cut(s)	217, 873, 1159, 1515, 1614, 2020
BsaXI	ACNNNNNCTCC	2 cut(s)	1493, 1523
Bsc4I	CCNNNNNNNGG	3 cut(s)	206, 374, 1850
Bse118I	RCCGGY	1 cut(s)	107
Bse1I	ACTGG	9 cut(s)	161, 256, 508, 584, 811, 999, 1116, 1458, 2059
Bse21I	CCTNAGG	1 cut(s)	1218
Bse8I	GATNNNNATC	2 cut(s)	309, 1605
BseBI	CCWGG	4 cut(s)	774, 1615, 1845, 1951
BseDI	CCNNGG	6 cut(s)	217, 873, 1159, 1515, 1614, 2020
BseGI	GGATG	6 cut(s)	293, 306, 403, 1013, 1131, 1951
BseJI	GATNNNNATC	2 cut(s)	309, 1605
BseLI	CCNNNNNNNGG	3 cut(s)	206, 374, 1850
BseMII	CTCAG	5 cut(s)	217, 410, 1232, 1251, 1345
BseNI	ACTGG	9 cut(s)	161, 256, 508, 584, 811, 999, 1116, 1458, 2059
BseRI	GAGGAG	2 cut(s)	1451, 1706
BseSI	GKGCMC	1 cut(s)	1881
BseXI	GCAGC	8 cut(s)	31, 256, 529, 836, 1281, 1360, 1607, 1909
BsgI	GTGCAG	2 cut(s)	444, 1564
Bsh1236I	CGCG	1 cut(s)	264
BshFI	GGCC	5 cut(s)	111, 608, 1164, 1216, 1656
BsiHKAI	GWGCWC	1 cut(s)	1146
BsiSI	CCGG	2 cut(s)	108, 1991
BslFI	GGGAC	4 cut(s)	350, 1024, 1495, 1742
BslI	CCNNNNNNNGG	3 cut(s)	206, 374, 1850
BsmAI	GTCTC	6 cut(s)	147, 497, 797, 1334, 1388, 2029
BsmFI	GGGAC	4 cut(s)	350, 1024, 1495, 1742
BsnI	GGCC	5 cut(s)	111, 608, 1164, 1216, 1656
Bso31I	GGTCTC	1 cut(s)	1388
Bsp1286I	GDGCHC	2 cut(s)	1146, 1881
Bsp19I	CCATGG	2 cut(s)	217, 1159
BspACI	CCGC	4 cut(s)	96, 200, 1274, 1657
BspANI	GGCC	5 cut(s)	111, 608, 1164, 1216, 1656
BspCNI	CTCAG	5 cut(s)	216, 411, 1231, 1250, 1344
BspFNI	CGCG	1 cut(s)	264
BspLI	GGNNCC	6 cut(s)	461, 1094, 1191, 1443, 1758, 1837
BspMAI	CTGCAG	2 cut(s)	519, 1597
BspPI	GGATC	4 cut(s)	318, 519, 620, 1265
BspQI	GCTCTTC	1 cut(s)	1710
BspTNI	GGTCTC	1 cut(s)	1388
BsrBI	CCGCTC	2 cut(s)	202, 1659
BsrFI	RCCGGY	1 cut(s)	107
BsrI	ACTGG	9 cut(s)	161, 256, 508, 584, 811, 999, 1116, 1458, 2059
BssAI	RCCGGY	1 cut(s)	107
BssECI	CCNNGG	6 cut(s)	217, 873, 1159, 1515, 1614, 2020
BssT1I	CCWWGG	5 cut(s)	217, 873, 1159, 1515, 2020
Bst2UI	CCWGG	4 cut(s)	774, 1615, 1845, 1951
Bst4CI	ACNGT	5 cut(s)	121, 334, 1118, 1706, 1798
Bst6I	CTCTTC	5 cut(s)	91, 332, 450, 1692, 1710
BstAPI	GCANNNNNTGC	1 cut(s)	2055
BstC8I	GCNNGC	5 cut(s)	109, 1086, 1661, 1909, 1929
BstDEI	CTNAG	5 cut(s)	203, 419, 1218, 1237, 1331
BstDSI	CCRYGG	2 cut(s)	217, 1159
BstF5I	GGATG	6 cut(s)	293, 306, 403, 1013, 1131, 1951
BstFNI	CGCG	1 cut(s)	264
BstH2I	RGCGCY	1 cut(s)	787
BstHHI	GCGC	3 cut(s)	264, 730, 786
BstMAI	GTCTC	6 cut(s)	147, 497, 797, 1334, 1388, 2029
BstMWI	GCNNNNNNNGC	5 cut(s)	104, 790, 1273, 1303, 2055
BstNI	CCWGG	4 cut(s)	774, 1615, 1845, 1951
BstNSI	RCATGY	2 cut(s)	1088, 1915
BstSCI	CCNGG	5 cut(s)	772, 1613, 1843, 1949, 1989
BstSFI	CTRYAG	3 cut(s)	515, 540, 1593
BstSLI	GKGCMC	1 cut(s)	1881
BstUI	CGCG	1 cut(s)	264
BstV1I	GCAGC	8 cut(s)	31, 256, 529, 836, 1281, 1360, 1607, 1909
BstV2I	GAAGAC	1 cut(s)	1581
BstX2I	RGATCY	2 cut(s)	81, 511
BstXI	CCANNNNNNTGG	2 cut(s)	616, 874
BstYI	RGATCY	2 cut(s)	81, 511
Bsu36I	CCTNAGG	1 cut(s)	1218
BsuI	GTATCC	1 cut(s)	1847
BsuRI	GGCC	5 cut(s)	111, 608, 1164, 1216, 1656
BtgI	CCRYGG	2 cut(s)	217, 1159
BtrI	CACGTC	1 cut(s)	2052
BtsCI	GGATG	6 cut(s)	293, 306, 403, 1013, 1131, 1951
BtsI	GCAGTG	4 cut(s)	186, 322, 867, 1435
Cac8I	GCNNGC	5 cut(s)	109, 1086, 1661, 1909, 1929
CfoI	GCGC	3 cut(s)	264, 730, 786
Cfr10I	RCCGGY	1 cut(s)	107
Cfr13I	GGNCC	4 cut(s)	536, 1189, 1756, 1953
CsiI	ACCWGGT	1 cut(s)	772
Csp6I	GTAC	4 cut(s)	164, 214, 1255, 1987
CspCI	CAANNNNNGTGG	2 cut(s)	567, 602
CviQI	GTAC	4 cut(s)	164, 214, 1255, 1987
DdeI	CTNAG	5 cut(s)	203, 419, 1218, 1237, 1331
DraIII	CACNNNGTG	1 cut(s)	2055
DriI	GACNNNNNGTC	1 cut(s)	587
EaeI	YGGCCR	1 cut(s)	1654
Eam1104I	CTCTTC	5 cut(s)	91, 332, 450, 1692, 1710
Eam1105I	GACNNNNNGTC	1 cut(s)	587
EarI	CTCTTC	5 cut(s)	91, 332, 450, 1692, 1710
Ecl136II	GAGCTC	1 cut(s)	1144
Eco130I	CCWWGG	5 cut(s)	217, 873, 1159, 1515, 2020
Eco24I	GRGCYC	1 cut(s)	1146
Eco31I	GGTCTC	1 cut(s)	1388
Eco47I	GGWCC	4 cut(s)	536, 1189, 1756, 1953
Eco47III	AGCGCT	1 cut(s)	785
Eco53kI	GAGCTC	1 cut(s)	1144
Eco57I	CTGAAG	3 cut(s)	1382, 1808, 1844
Eco81I	CCTNAGG	1 cut(s)	1218
EcoICRI	GAGCTC	1 cut(s)	1144
EcoO109I	RGGNCCY	2 cut(s)	1189, 1756
EcoRII	CCWGG	4 cut(s)	772, 1613, 1843, 1949
EcoT14I	CCWWGG	5 cut(s)	217, 873, 1159, 1515, 2020
EcoT22I	ATGCAT	1 cut(s)	471
EcoT38I	GRGCYC	1 cut(s)	1146
ErhI	CCWWGG	5 cut(s)	217, 873, 1159, 1515, 2020
FaqI	GGGAC	4 cut(s)	350, 1024, 1495, 1742
FauNDI	CATATG	2 cut(s)	1378, 1830
FbaI	TGATCA	1 cut(s)	1003
FokI	GGATG	6 cut(s)	300, 313, 410, 1000, 1138, 1958
FriOI	GRGCYC	1 cut(s)	1146
FspBI	CTAG	4 cut(s)	573, 1416, 1446, 2021
GlaI	GCGC	3 cut(s)	263, 729, 785
GsuI	CTGGAG	2 cut(s)	1475, 1985
HaeII	RGCGCY	1 cut(s)	787
HaeIII	GGCC	5 cut(s)	111, 608, 1164, 1216, 1656
HapII	CCGG	2 cut(s)	108, 1991
HhaI	GCGC	3 cut(s)	264, 730, 786
Hin6I	GCGC	3 cut(s)	262, 728, 784
HinP1I	GCGC	3 cut(s)	262, 728, 784
HincII	GTYRAC	1 cut(s)	387
HindII	GTYRAC	1 cut(s)	387
HindIII	AAGCTT	2 cut(s)	1265, 1772
HpaII	CCGG	2 cut(s)	108, 1991
HphI	GGTGA	6 cut(s)	613, 695, 889, 928, 1415, 1873
Hpy166II	GTNNAC	4 cut(s)	387, 570, 919, 1451
Hpy188I	TCNGA	7 cut(s)	147, 420, 531, 815, 1240, 1611, 2028
Hpy8I	GTNNAC	4 cut(s)	387, 570, 919, 1451
Hpy99I	CGWCG	1 cut(s)	2056
HpyAV	CCTTC	4 cut(s)	65, 598, 1293, 1357
HpyCH4III	ACNGT	5 cut(s)	121, 334, 1118, 1706, 1798
HpyCH4IV	ACGT	2 cut(s)	63, 2051
HpyF10VI	GCNNNNNNNGC	5 cut(s)	104, 790, 1273, 1303, 2055
HpyF3I	CTNAG	5 cut(s)	203, 419, 1218, 1237, 1331
HpySE526I	ACGT	2 cut(s)	63, 2051
HspAI	GCGC	3 cut(s)	262, 728, 784
KroI	GCCGGC	1 cut(s)	107
KroNI	GCCGGC	1 cut(s)	109
Ksp22I	TGATCA	1 cut(s)	1003
LguI	GCTCTTC	1 cut(s)	1710
LmnI	GCTCC	2 cut(s)	1141, 1303
Lsp1109I	GCAGC	8 cut(s)	31, 256, 529, 836, 1281, 1360, 1607, 1909
LweI	GCATC	7 cut(s)	971, 985, 1048, 1281, 1628, 1695, 1936
MabI	ACCWGGT	1 cut(s)	772
MaeI	CTAG	4 cut(s)	573, 1416, 1446, 2021
MaeII	ACGT	2 cut(s)	63, 2051
MaeIII	GTNAC	3 cut(s)	121, 1027, 1782
MbiI	CCGCTC	2 cut(s)	202, 1659
MfeI	CAATTG	1 cut(s)	1152
MflI	RGATCY	2 cut(s)	81, 511
MhlI	GDGCHC	2 cut(s)	1146, 1881
MluCI	AATT	6 cut(s)	19, 630, 974, 1152, 1421, 1461
MlyI	GAGTC	3 cut(s)	431, 925, 1376
MmeI	TCCRAC	1 cut(s)	236
Mph1103I	ATGCAT	1 cut(s)	471
MroNI	GCCGGC	1 cut(s)	107
MseI	TTAA	2 cut(s)	900, 1071
MslI	CAYNNNNRTG	2 cut(s)	614, 1884
MspI	CCGG	2 cut(s)	108, 1991
MspR9I	CCNGG	5 cut(s)	774, 1615, 1845, 1951, 1991
MunI	CAATTG	1 cut(s)	1152
MvaI	CCWGG	4 cut(s)	774, 1615, 1845, 1951
MvnI	CGCG	1 cut(s)	264
MwoI	GCNNNNNNNGC	5 cut(s)	104, 790, 1273, 1303, 2055
NaeI	GCCGGC	1 cut(s)	109
NciI	CCSGG	1 cut(s)	1991
NcoI	CCATGG	2 cut(s)	217, 1159
NdeI	CATATG	2 cut(s)	1378, 1830
NgoMIV	GCCGGC	1 cut(s)	107
NlaIV	GGNNCC	6 cut(s)	461, 1094, 1191, 1443, 1758, 1837
NmuCI	GTSAC	2 cut(s)	121, 1782
NsiI	ATGCAT	1 cut(s)	471
NspI	RCATGY	2 cut(s)	1088, 1915
OliI	CACNNNNGTG	1 cut(s)	1884
PaeI	GCATGC	1 cut(s)	1088
PciSI	GCTCTTC	1 cut(s)	1710
PdiI	GCCGGC	1 cut(s)	109
PfeI	GAWTC	7 cut(s)	14, 810, 1066, 1207, 1315, 1342, 1523
PflFI	GACNNNGTC	1 cut(s)	1118
PflMI	CCANNNNNTGG	1 cut(s)	374
PleI	GAGTC	3 cut(s)	431, 925, 1375
PpsI	GAGTC	3 cut(s)	431, 925, 1375
PpuMI	RGGWCCY	2 cut(s)	1189, 1756
Psp124BI	GAGCTC	1 cut(s)	1146
Psp1406I	AACGTT	1 cut(s)	63
Psp5II	RGGWCCY	2 cut(s)	1189, 1756
Psp6I	CCWGG	4 cut(s)	772, 1613, 1843, 1949
PspGI	CCWGG	4 cut(s)	772, 1613, 1843, 1949
PspN4I	GGNNCC	6 cut(s)	461, 1094, 1191, 1443, 1758, 1837
PspPI	GGNCC	4 cut(s)	536, 1189, 1756, 1953
PspPPI	RGGWCCY	2 cut(s)	1189, 1756
PstI	CTGCAG	2 cut(s)	519, 1597
PsuI	RGATCY	2 cut(s)	81, 511
PsyI	GACNNNGTC	1 cut(s)	1118
RsaI	GTAC	4 cut(s)	165, 215, 1256, 1988
RsaNI	GTAC	4 cut(s)	164, 214, 1255, 1987
RseI	CAYNNNNRTG	2 cut(s)	614, 1884
SacI	GAGCTC	1 cut(s)	1146
SapI	GCTCTTC	1 cut(s)	1710
SaqAI	TTAA	2 cut(s)	900, 1071
Sau96I	GGNCC	4 cut(s)	536, 1189, 1756, 1953
SchI	GAGTC	3 cut(s)	431, 925, 1376
ScrFI	CCNGG	5 cut(s)	774, 1615, 1845, 1951, 1991
SduI	GDGCHC	2 cut(s)	1146, 1881
SexAI	ACCWGGT	1 cut(s)	772
SfaNI	GCATC	7 cut(s)	971, 985, 1048, 1281, 1628, 1695, 1936
SfcI	CTRYAG	3 cut(s)	515, 540, 1593
SinI	GGWCC	4 cut(s)	536, 1189, 1756, 1953
SmiMI	CAYNNNNRTG	2 cut(s)	614, 1884
SmlI	CTYRAG	2 cut(s)	1250, 1277
SmoI	CTYRAG	2 cut(s)	1250, 1277
SphI	GCATGC	1 cut(s)	1088
Sse9I	AATT	6 cut(s)	19, 630, 974, 1152, 1421, 1461
SsiI	CCGC	4 cut(s)	96, 200, 1274, 1657
SspMI	CTAG	4 cut(s)	573, 1416, 1446, 2021
SstI	GAGCTC	1 cut(s)	1146
StyD4I	CCNGG	5 cut(s)	772, 1613, 1843, 1949, 1989
StyI	CCWWGG	5 cut(s)	217, 873, 1159, 1515, 2020
TaaI	ACNGT	5 cut(s)	121, 334, 1118, 1706, 1798
TaiI	ACGT	2 cut(s)	66, 2054
TaqI	TCGA	3 cut(s)	17, 1318, 1689
TasI	AATT	6 cut(s)	19, 630, 974, 1152, 1421, 1461
TatI	WGTACW	2 cut(s)	163, 1254
TauI	GCSGC	2 cut(s)	98, 1659
TfiI	GAWTC	7 cut(s)	14, 810, 1066, 1207, 1315, 1342, 1523
Tru1I	TTAA	2 cut(s)	900, 1071
Tru9I	TTAA	2 cut(s)	900, 1071
TseFI	GTSAC	2 cut(s)	121, 1782
TseI	GCWGC	8 cut(s)	44, 244, 517, 824, 1294, 1373, 1595, 1897
Tsp45I	GTSAC	2 cut(s)	121, 1782
TspDTI	ATGAA	5 cut(s)	468, 629, 729, 967, 1199
TspGWI	ACGGA	1 cut(s)	1866
Tth111I	GACNNNGTC	1 cut(s)	1118
Van91I	CCANNNNNTGG	1 cut(s)	374
VpaK11BI	GGWCC	4 cut(s)	536, 1189, 1756, 1953
XapI	RAATTY	2 cut(s)	19, 1421
XceI	RCATGY	2 cut(s)	1088, 1915
XmaJI	CCTAGG	1 cut(s)	2020
XspI	CTAG	4 cut(s)	573, 1416, 1446, 2021
Zsp2I	ATGCAT	1 cut(s)	471

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.