MD17G1066800.v1.1

Phosphatidylinositol 4-kinase gamma 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001564
Physical Location & Seq
Forward (+)
5392596 .. 5395691
3096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1066800.v1.1.491

Sequence Viewer

Length: 1917 bp
ATGTCTCCTGAATTGCATAAACCTGTACATAACCAGATGGCAGTTGCAACCTTCAAAGGACCTCTTAATGGTGAGTACCATGGGGATAAGAGGTTTGAGAGAAAAAATTCTGGCAAGAGACGTGTATTTGTGCAGACTGAAGCCGGTTCTGTTTTGGGTATAGAGCTAGATAGTAGTGACAATGCTCACACGGTGAAGAGGAAATTGCGGCTTGCACTAAATGTCCCAACTGATGAGAGTTCATTGGTATGTGGTGACATGGTGTTGAAGAATGATCTCAGCATTGTTAGAAATGATTCTCCGCTTCTTCTCACCAAGAACTTTATGCACAGAAGCTCATCTACCCCTTGTCTCTCACCTACTGGGAAGGATCTTCAGCAGAGGGATCAGAGTGGTCCAATTGAGGTACTGGGATACTCAGATCAGTTTGCCAAAACAAAAAAACTGGTTCGTGATATTGTGAAGGCTATACGAAATGACATTGATCCCGTTCCTGTTCACAGTGGTCTTGGAGGTGGATATTTCTTTAGAAATTGCGATGGTGAAAATATTGCCATTGTGAAACCGACGGATGAGGAACCGTATGCTCCAAACAACCCAAAAGGTTTTGTTGGGAAAGCTCTTGGACAACCAGGTCTCAAACGTTCTGTGCGCGTTGGGGAGACAGGTTTTAGAGAAGTTGCAGCATTCCTTCTTGATTACAACAATTTTGCTAACGTCCCTGCCACTGCTCTTGTGAAAATTACGCATTCAGTTTTTAATGTTAATGATGGAGTGAAGGTTAATATGCATCAAAATGGAAAGCAAGCAAGTAAGATTGCATCATTGCAGCAATTCGTTCCTCATGATTTTGATGCCAGTGATCACGGGACTTCTAGCTTCCCTGTTGCTGCTGTGCACAGAATAGGTATCCTAGATATCCGGATTCTTAACACAGACAGGCATGCCGGGAACCTTCTTGTTAGGAAGCTTGATGGTCCTGGGAAGTTTGGTCAGGTGGAGCTTGTACCCATTGATCATGGCTTTTGTCTGCCCGAAAGCTTGGAGGATCCATACTTTGAGTGGATACACTGGCCTCAGGCATCAATTCCTTTCTCTGATGATGAGCTTGCATATATCGATAAACTTGACCCTTTCAAAGACTCTGATATGCTTAGGATGGAGCTGCCCATGATTCGAGAGGCATGCCTACGAGTCTTGGTTCTTTGCACGATTTTCCTCAAGGGAGCTGCAGCTTTTGGCCTCTGTCTTTCCGAGATTGGTGAGATGATGAGTAGGGAATTTTGTGGGCACGAGGAGGAGCCGAGTGAACTTGAGGTAATATGCATTGAAGCAAAGAAACTGTTAGAGGAGAATATATATTGTGTGCCAAAAGCAATCAAGGAGGTGGTTCAATTTGATCTAGACTGTGGGGAGGAGTCAGATTTGAGTCCAATCACGGAAGTGAAAATGGAAACTGAGAAGAGGAATTTCCATTGTGGATCAAAAGGTGGAAACGGAAGATATGCACTTTCCAAACTAGAGGAGAGTGTGGAGGAGGACATGGATGGGCTCAACGGTAATGAATTACCTTCGAGGTTGGATGAATGTGCGGGTGTGGTGCAGGATCGGATTCCAAATGTTTCCAAGCTGTCAGCATCATTGAGAAACATCAGCATTGGTGAGAAGAGTTGGCGACACCTAGGTTTGAAGCAAAAGAACGGTTGTTGGGCTGGCTCATCGTCTGGAAACAGGAGTGTGAATGAGCAGCTACCTGTGAGCAGCAGTTTCGTGAAGCTGGCGGATATGAACAAAGAGGAATGGATGCAGTTTCTTGAGAATTTCCAGAGATTGCTCCGTCCTGCTTTCGCTAATCGCAAAGCAGGGAATCGAGGTCAGAGGCAGAGACAGAGGCTAGGGACTTCATGCCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

639

Amino Acids

70.95

Weight (kDa)

6.23

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 184 - 418 2.4e-42 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 633
AccII CGCG 1 cut(s) 654
AccIII TCCGGA 1 cut(s) 921
AciI CCGC 4 cut(s) 208, 302, 1592, 1781
AclI AACGTT 1 cut(s) 643
AclWI GGATC 7 cut(s) 378, 393, 479, 1043, 1056, 1489, 1614
AcsI RAATTY 4 cut(s) 106, 1280, 1468, 1819
AcuI CTGAAG 2 cut(s) 159, 359
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 4 cut(s) 27, 77, 408, 1008
AfiI CCNNNNNNNGG 1 cut(s) 68
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 6 cut(s) 55, 268, 1138, 1331, 1394, 1690
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 2 cut(s) 631, 979
AleI CACNNNNGTG 1 cut(s) 1442
Alw21I GWGCWC 1 cut(s) 900
Alw26I GTCTC 6 cut(s) 9, 112, 356, 641, 656, 1879
Alw44I GTGCAC 1 cut(s) 896
AlwI GGATC 7 cut(s) 378, 393, 479, 1043, 1056, 1489, 1614
Aor13HI TCCGGA 1 cut(s) 921
AoxI GGCC 2 cut(s) 1073, 1240
ApaLI GTGCAC 1 cut(s) 896
ApeKI GCWGC 8 cut(s) 683, 829, 890, 1165, 1229, 1232, 1747, 1761
ApoI RAATTY 4 cut(s) 106, 1280, 1468, 1819
Asp700I GAANNNNTTC 2 cut(s) 106, 295
AspA2I CCTAGG 1 cut(s) 1681
AspLEI GCGC 1 cut(s) 654
AspS9I GGNCC 3 cut(s) 59, 395, 977
AsuC2I CCSGG 1 cut(s) 949
AsuHPI GGTGA 8 cut(s) 83, 205, 266, 304, 348, 554, 1274, 1673
AvaII GGWCC 3 cut(s) 59, 395, 977
AvrII CCTAGG 1 cut(s) 1681
AxyI CCTNAGG 1 cut(s) 1077
BaeGI GKGCMC 2 cut(s) 900, 1293
BamHI GGATCC 1 cut(s) 1048
BanII GRGCYC 1 cut(s) 1554
BarI GAAGNNNNNNTAC 2 cut(s) 325, 357
BauI CACGAG 1 cut(s) 1292
Bbv12I GWGCWC 1 cut(s) 900
BbvI GCAGC 8 cut(s) 695, 841, 877, 1152, 1216, 1244, 1759, 1773
BccI CCATC 6 cut(s) 31, 533, 764, 968, 1153, 1541
BcgI CGANNNNNNTGC 4 cut(s) 1167, 1201, 1750, 1784
BciT130I CCWGG 2 cut(s) 633, 981
BciVI GTATCC 3 cut(s) 407, 920, 1059
BclI TGATCA 2 cut(s) 862, 1015
BcnI CCSGG 1 cut(s) 949
BcoDI GTCTC 6 cut(s) 9, 112, 356, 641, 656, 1879
BfaI CTAG 7 cut(s) 167, 876, 914, 1403, 1520, 1682, 1895
BfmI CTRYAG 1 cut(s) 1230
BfuI GTATCC 3 cut(s) 407, 920, 1059
BisI GCNGC 9 cut(s) 209, 684, 830, 891, 1166, 1230, 1233, 1748, 1762
BlnI CCTAGG 1 cut(s) 1681
BlsI GCNGC 9 cut(s) 210, 685, 831, 892, 1167, 1231, 1234, 1749, 1763
Bme1390I CCNGG 3 cut(s) 633, 949, 981
Bme18I GGWCC 3 cut(s) 59, 395, 977
BmgBI CACGTC 1 cut(s) 122
BmgT120I GGNCC 3 cut(s) 59, 395, 977
BmiI GGNNCC 4 cut(s) 579, 953, 1050, 1302
BmrFI CCNGG 3 cut(s) 633, 949, 981
BmrI ACTGGG 2 cut(s) 372, 419
BmsI GCATC 6 cut(s) 799, 830, 844, 1091, 1646, 1794
BmuI ACTGGG 2 cut(s) 372, 419
Bpu10I CCTNAGC 1 cut(s) 1154
BpuEI CTTGAG 3 cut(s) 1205, 1334, 1835
BpuMI CCSGG 1 cut(s) 949
Bsa29I ATCGAT 1 cut(s) 1119
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 3 cut(s) 79, 980, 1681
BsaWI WCCGGW 1 cut(s) 921
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 6 cut(s) 367, 414, 450, 858, 1076, 1909
Bse21I CCTNAGG 1 cut(s) 1077
Bse3DI GCAATG 1 cut(s) 824
BseAI TCCGGA 1 cut(s) 921
BseBI CCWGG 2 cut(s) 633, 981
BseCI ATCGAT 1 cut(s) 1119
BseDI CCNNGG 3 cut(s) 79, 980, 1681
BseGI GGATG 5 cut(s) 577, 1164, 1552, 1588, 1809
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMI GCAATG 1 cut(s) 824
BseMII CTCAG 4 cut(s) 292, 432, 1091, 1449
BseNI ACTGG 6 cut(s) 367, 414, 450, 858, 1076, 1909
BseRI GAGGAG 6 cut(s) 1310, 1313, 1364, 1430, 1538, 1550
BseSI GKGCMC 2 cut(s) 900, 1293
BseXI GCAGC 8 cut(s) 695, 841, 877, 1152, 1216, 1244, 1759, 1773
BsgI GTGCAG 2 cut(s) 152, 1622
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 2 cut(s) 1075, 1242
BshVI ATCGAT 1 cut(s) 1119
BsiHKAI GWGCWC 1 cut(s) 900
BsiSI CCGG 3 cut(s) 144, 922, 948
BslFI GGGAC 4 cut(s) 209, 704, 883, 1912
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 6 cut(s) 9, 112, 356, 641, 656, 1879
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 4 cut(s) 209, 704, 883, 1912
BsmI GAATGC 2 cut(s) 686, 748
BsnI GGCC 2 cut(s) 1075, 1242
Bso31I GGTCTC 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 900, 1293, 1554
Bsp13I TCCGGA 1 cut(s) 921
Bsp1407I TGTACA 1 cut(s) 25
Bsp19I CCATGG 1 cut(s) 79
BspACI CCGC 4 cut(s) 208, 302, 1592, 1781
BspANI GGCC 2 cut(s) 1075, 1242
BspCNI CTCAG 4 cut(s) 291, 431, 1090, 1450
BspDI ATCGAT 1 cut(s) 1119
BspEI TCCGGA 1 cut(s) 921
BspFNI CGCG 1 cut(s) 654
BspHI TCATGA 1 cut(s) 844
BspLI GGNNCC 4 cut(s) 579, 953, 1050, 1302
BspMAI CTGCAG 1 cut(s) 1234
BspPI GGATC 7 cut(s) 378, 393, 479, 1043, 1056, 1489, 1614
BspTNI GGTCTC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 824
BsrFI RCCGGY 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 25
BsrI ACTGG 6 cut(s) 367, 414, 450, 858, 1076, 1909
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 79, 980, 1681
BssSI CACGAG 1 cut(s) 1292
BssT1I CCWWGG 2 cut(s) 79, 1681
Bst2BI CACGAG 1 cut(s) 1292
Bst2UI CCWGG 2 cut(s) 633, 981
Bst4CI ACNGT 7 cut(s) 193, 503, 582, 1344, 1409, 1559, 1703
Bst6I CTCTTC 3 cut(s) 191, 1457, 1661
BstAUI TGTACA 1 cut(s) 25
BstC8I GCNNGC 7 cut(s) 213, 807, 945, 1110, 1186, 1714, 1779
BstDEI CTNAG 5 cut(s) 278, 418, 1077, 1154, 1458
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 5 cut(s) 577, 1164, 1552, 1588, 1809
BstFNI CGCG 1 cut(s) 654
BstHHI GCGC 1 cut(s) 654
BstMAI GTCTC 6 cut(s) 9, 112, 356, 641, 656, 1879
BstNI CCWGG 2 cut(s) 633, 981
BstNSI RCATGY 2 cut(s) 947, 1188
BstSCI CCNGG 3 cut(s) 631, 947, 979
BstSFI CTRYAG 1 cut(s) 1230
BstSLI GKGCMC 2 cut(s) 900, 1293
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 8 cut(s) 695, 841, 877, 1152, 1216, 1244, 1759, 1773
BstX2I RGATCY 2 cut(s) 370, 1048
BstYI RGATCY 2 cut(s) 370, 1048
Bsu15I ATCGAT 1 cut(s) 1119
Bsu36I CCTNAGG 1 cut(s) 1077
BsuI GTATCC 3 cut(s) 407, 920, 1059
BsuRI GGCC 2 cut(s) 1075, 1242
BsuTUI ATCGAT 1 cut(s) 1119
BtgI CCRYGG 1 cut(s) 79
BtgZI GCGATG 1 cut(s) 552
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 5 cut(s) 577, 1164, 1552, 1588, 1809
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 4 cut(s) 508, 726, 865, 1069
Cac8I GCNNGC 7 cut(s) 213, 807, 945, 1110, 1186, 1714, 1779
CciI TCATGA 1 cut(s) 844
CfoI GCGC 1 cut(s) 654
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 3 cut(s) 59, 395, 977
ClaI ATCGAT 1 cut(s) 1119
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 4 cut(s) 26, 76, 407, 1007
CspCI CAANNNNNGTGG 2 cut(s) 715, 750
CviAII CATG 9 cut(s) 80, 259, 845, 944, 1019, 1171, 1185, 1543, 1905
CviQI GTAC 4 cut(s) 26, 76, 407, 1007
DdeI CTNAG 5 cut(s) 278, 418, 1077, 1154, 1458
DraIII CACNNNGTG 1 cut(s) 193
DrdI GACNNNNNNGTC 1 cut(s) 633
DseDI GACNNNNNNGTC 1 cut(s) 633
Eam1104I CTCTTC 3 cut(s) 191, 1457, 1661
EarI CTCTTC 3 cut(s) 191, 1457, 1661
EciI GGCGGA 1 cut(s) 1796
Eco130I CCWWGG 2 cut(s) 79, 1681
Eco24I GRGCYC 1 cut(s) 1554
Eco31I GGTCTC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 919
Eco47I GGWCC 3 cut(s) 59, 395, 977
Eco57I CTGAAG 2 cut(s) 159, 359
Eco81I CCTNAGG 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 631, 979
EcoRV GATATC 1 cut(s) 919
EcoT14I CCWWGG 2 cut(s) 79, 1681
EcoT22I ATGCAT 2 cut(s) 792, 1328
EcoT38I GRGCYC 1 cut(s) 1554
ErhI CCWWGG 2 cut(s) 79, 1681
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 9 cut(s) 83, 262, 848, 947, 1022, 1174, 1188, 1546, 1908
FalI AAGNNNNNCTT 2 cut(s) 48, 80
FaqI GGGAC 4 cut(s) 209, 704, 883, 1912
FatI CATG 9 cut(s) 79, 258, 844, 943, 1018, 1170, 1184, 1542, 1904
FauI CCCGC 1 cut(s) 1585
FbaI TGATCA 2 cut(s) 862, 1015
Fnu4HI GCNGC 9 cut(s) 209, 684, 830, 891, 1166, 1230, 1233, 1748, 1762
FokI GGATG 5 cut(s) 584, 1171, 1559, 1595, 1816
FriOI GRGCYC 1 cut(s) 1554
Fsp4HI GCNGC 9 cut(s) 209, 684, 830, 891, 1166, 1230, 1233, 1748, 1762
FspBI CTAG 7 cut(s) 167, 876, 914, 1403, 1520, 1682, 1895
GlaI GCGC 1 cut(s) 653
GluI GCNGC 9 cut(s) 209, 684, 830, 891, 1166, 1230, 1233, 1748, 1762
HaeIII GGCC 2 cut(s) 1075, 1242
HapII CCGG 3 cut(s) 144, 922, 948
HhaI GCGC 1 cut(s) 654
Hin1II CATG 9 cut(s) 83, 262, 848, 947, 1022, 1174, 1188, 1546, 1908
Hin6I GCGC 1 cut(s) 652
HinP1I GCGC 1 cut(s) 652
HindIII AAGCTT 2 cut(s) 968, 1039
HinfI GANTC 9 cut(s) 296, 925, 1142, 1174, 1194, 1418, 1429, 1612, 1867
HpaII CCGG 3 cut(s) 144, 922, 948
HphI GGTGA 8 cut(s) 83, 205, 266, 304, 348, 554, 1274, 1673
Hpy166II GTNNAC 3 cut(s) 499, 898, 1310
Hpy188I TCNGA 8 cut(s) 390, 421, 1099, 1147, 1255, 1423, 1611, 1878
Hpy8I GTNNAC 3 cut(s) 499, 898, 1310
Hpy99I CGWCG 1 cut(s) 571
HpyAV CCTTC 7 cut(s) 61, 361, 457, 701, 772, 965, 1581
HpyCH4III ACNGT 7 cut(s) 193, 503, 582, 1344, 1409, 1559, 1703
HpyCH4IV ACGT 3 cut(s) 121, 643, 717
HpyF3I CTNAG 5 cut(s) 278, 418, 1077, 1154, 1458
HpySE526I ACGT 3 cut(s) 121, 643, 717
Hsp92II CATG 9 cut(s) 83, 262, 848, 947, 1022, 1174, 1188, 1546, 1908
HspAI GCGC 1 cut(s) 652
Kpn2I TCCGGA 1 cut(s) 921
Ksp22I TGATCA 2 cut(s) 862, 1015
LmnI GCTCC 6 cut(s) 592, 1000, 1162, 1226, 1300, 1839
Lsp1109I GCAGC 8 cut(s) 695, 841, 877, 1152, 1216, 1244, 1759, 1773
LweI GCATC 6 cut(s) 799, 830, 844, 1091, 1646, 1794
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 7 cut(s) 167, 876, 914, 1403, 1520, 1682, 1895
MaeII ACGT 3 cut(s) 121, 643, 717
MaeIII GTNAC 2 cut(s) 176, 254
MboII GAAGA 7 cut(s) 208, 280, 299, 365, 1474, 1512, 1678
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 2 cut(s) 370, 1048
MhlI GDGCHC 3 cut(s) 900, 1293, 1554
MlyI GAGTC 4 cut(s) 1136, 1203, 1427, 1438
MmeI TCCRAC 1 cut(s) 1560
Mph1103I ATGCAT 2 cut(s) 792, 1328
MroI TCCGGA 1 cut(s) 921
MroXI GAANNNNTTC 2 cut(s) 106, 295
MseI TTAA 5 cut(s) 66, 759, 765, 783, 930
MslI CAYNNNNRTG 3 cut(s) 247, 795, 1442
MspI CCGG 3 cut(s) 144, 922, 948
MspR9I CCNGG 3 cut(s) 633, 949, 981
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 2 cut(s) 686, 748
MvaI CCWGG 2 cut(s) 633, 981
MvnI CGCG 1 cut(s) 654
NciI CCSGG 1 cut(s) 949
NcoI CCATGG 1 cut(s) 79
NlaIII CATG 9 cut(s) 83, 262, 848, 947, 1022, 1174, 1188, 1546, 1908
NlaIV GGNNCC 4 cut(s) 579, 953, 1050, 1302
NmeAIII GCCGAG 1 cut(s) 1329
NmuCI GTSAC 2 cut(s) 176, 254
NsiI ATGCAT 2 cut(s) 792, 1328
NspI RCATGY 2 cut(s) 947, 1188
OliI CACNNNNGTG 1 cut(s) 1442
PaeI GCATGC 2 cut(s) 947, 1188
PagI TCATGA 1 cut(s) 844
PctI GAATGC 2 cut(s) 686, 748
PdmI GAANNNNTTC 2 cut(s) 106, 295
PfeI GAWTC 5 cut(s) 296, 925, 1174, 1612, 1867
PkrI GCNGC 9 cut(s) 210, 685, 831, 892, 1167, 1231, 1234, 1749, 1763
PleI GAGTC 4 cut(s) 1136, 1202, 1426, 1437
PpsI GAGTC 4 cut(s) 1136, 1202, 1426, 1437
PpuMI RGGWCCY 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 643
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 631, 979
PspGI CCWGG 2 cut(s) 631, 979
PspN4I GGNNCC 4 cut(s) 579, 953, 1050, 1302
PspPI GGNCC 3 cut(s) 59, 395, 977
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 1 cut(s) 1234
PsuI RGATCY 2 cut(s) 370, 1048
RsaI GTAC 4 cut(s) 27, 77, 408, 1008
RsaNI GTAC 4 cut(s) 26, 76, 407, 1007
RseI CAYNNNNRTG 3 cut(s) 247, 795, 1442
SaqAI TTAA 5 cut(s) 66, 759, 765, 783, 930
SatI GCNGC 9 cut(s) 209, 684, 830, 891, 1166, 1230, 1233, 1748, 1762
Sau96I GGNCC 3 cut(s) 59, 395, 977
SchI GAGTC 4 cut(s) 1136, 1203, 1427, 1438
ScrFI CCNGG 3 cut(s) 633, 949, 981
SduI GDGCHC 3 cut(s) 900, 1293, 1554
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 6 cut(s) 799, 830, 844, 1091, 1646, 1794
SfcI CTRYAG 1 cut(s) 1230
SinI GGWCC 3 cut(s) 59, 395, 977
SmiMI CAYNNNNRTG 3 cut(s) 247, 795, 1442
SmlI CTYRAG 3 cut(s) 1220, 1313, 1814
SmoI CTYRAG 3 cut(s) 1220, 1313, 1814
SphI GCATGC 2 cut(s) 947, 1188
SsiI CCGC 4 cut(s) 208, 302, 1592, 1781
SspI AATATT 1 cut(s) 550
SspMI CTAG 7 cut(s) 167, 876, 914, 1403, 1520, 1682, 1895
StyD4I CCNGG 3 cut(s) 631, 947, 979
StyI CCWWGG 2 cut(s) 79, 1681
TaaI ACNGT 7 cut(s) 193, 503, 582, 1344, 1409, 1559, 1703
TaiI ACGT 3 cut(s) 124, 646, 720
TaqI TCGA 4 cut(s) 1119, 1177, 1574, 1870
TatI WGTACW 1 cut(s) 25
TauI GCSGC 1 cut(s) 211
TfiI GAWTC 5 cut(s) 296, 925, 1174, 1612, 1867
Tru1I TTAA 5 cut(s) 66, 759, 765, 783, 930
Tru9I TTAA 5 cut(s) 66, 759, 765, 783, 930
TscAI CASTG 4 cut(s) 508, 733, 865, 1076
TseFI GTSAC 2 cut(s) 176, 254
TseI GCWGC 8 cut(s) 683, 829, 890, 1165, 1229, 1232, 1747, 1761
Tsp45I GTSAC 2 cut(s) 176, 254
TspDTI ATGAA 5 cut(s) 231, 1578, 1599, 1802, 1893
TspGWI ACGGA 4 cut(s) 584, 1454, 1512, 1826
TspRI CASTG 4 cut(s) 508, 733, 865, 1076
VneI GTGCAC 1 cut(s) 896
VpaK11BI GGWCC 3 cut(s) 59, 395, 977
XapI RAATTY 4 cut(s) 106, 1280, 1468, 1819
XbaI TCTAGA 1 cut(s) 1402
XceI RCATGY 2 cut(s) 947, 1188
XcmI CCANNNNNNNNNTGG 1 cut(s) 1059
XmaJI CCTAGG 1 cut(s) 1681
XmnI GAANNNNTTC 2 cut(s) 106, 295
XspI CTAG 7 cut(s) 167, 876, 914, 1403, 1520, 1682, 1895
Zsp2I ATGCAT 2 cut(s) 792, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.