Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0001564

Physical Location & Seq

Genomic Coordinates Reverse (-)

58869198 .. 58871108

Gene Structure 1911 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00378380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGTCTCGTAAGCTGGATAGCCCCGTACAGACCCAAATGGCAGTGGCATTATTTAACAGCCCGCTTGCCAGTGAGTATGGGAGCAAGAGGGTGGAGGGGAAGCAGCCTGCTGGTCGGAGAAGAGTTTATGTGCAAACTGAAACTGGATGTGTCCTCGGGATGGAATTAGATCGCGGTGACAATGCCCATACTGTGAAGAGGAGATTGCAGCTTGCTCTCAATGTTCCAACTGAAGAGAGCTCGTTGACATTTGGGGATATGGTGTTGAAGAACGATCTCAGTGCAGTTCGAAATGATTCCCCTCTTCTTCTTACACGTAACCTTATGCACAGAAGCTCATCAACTCCATGTTTATCACCTACTGGGAGGGATATGCAGCAGAGAGATTGGAGTGGTCCTATAGTGATATTAGGGAACTCAGATCAGTTTGCTCGAACAAAGCATGTGGTCAAGGACATTAAGAAGGCCATCAAGATGGGGGTTGATCCAATTCCTGTGCATAGTGGGCTTGGAGGTGCATACTATTTTAGGAATAGTAGAGGTGAAAGTGTTGCAATAGTGAAGCCAACTGATGAAGAACCTTTTGCCCCGAATAACCCAAAAGGTTTTGTTGGTAAAGCTCTGGGCCAACCAGGTTTGAAGCGTTCAGTGCGGGTTGGGGAAACTGGTTTCAGAGAAGTTGCAGCTTATCTGCTCGACAAAGATCACTCTGCAAATGTGCCTCCCACTGCCTTGGTGAAGATCACTCACTCAATCTTCAACATAAATGATGGGGTGAATGGGAACAAGCTGCAAAAGAAGAGGCTGGTTAGCAAGATTGCATCTTGCCAACAATACATACAACATGATTTTGATGCCAGTGATCATGGGACATCCAGCTTTCCAGTAGCAGCTGTGCATCGCATAGGGATTTTAGACATTAGGATTTTAAACACAGACAGGCATGCAGGGAACCTCTTAGTTAGGAAACTTGATGGTGTTGGGAGGTTTGGTCAAGTGGACCTCATTCCGATTGACCATGGCCTTTGCTTGCCAGAATGTTTGGAGGACCCGTACTTTGAGTGGATACATTGGCCTCAGGCTTCTATTCCATTCTCAGATGACGAGCTTGATTACATAAAAAAGCTTGATCCGGATGAGGAATGTGAGATGCTGCGAAGGGAGCTTCCCATGATACGAGAGGCTTGTCTCAGGGTTTTGGTTCTCTGCACCATTTTTCTGAAGGAGTCTGCTGCTTATGGTCTCTGTCTTGCTGAAATAGGTGAGATGATGACTAGGGAATTTCGTAGTGGCGAGGAGGAGCCTAGTGAACTTGAGGTGATTTGCATGGAAGCAAGGAGGATGGTAGCGGAAAGGGAGGTGCCGTCCCCCAAGGCTGATGTGGGAGCTGAGGAGTTCCTATTTGATATAGATTGTGAGGACGCGGAATCAGACTTCAAATCTGCAGCAGAGGATTATGTGATCAGGACAGCTTTTCCATTTGGAAATGGGAGTGGACATGGTCGCTCTCCTCTCTCTAAACTGGAAGAAAGCATTGAGGAGGAAGAGGAAAGTGAAGGAGAAGAACAAGAGGTGTTAGCAAGTCTGCCACCTTCTGAACATATCCCATCAGTAGCAAAGCTTTCTATGTCGCTGAAGACTACAAGTTTGGGTGAAAAGAACCTCCTGAAATATGTGGGAGCAAAAACAGAAAATGGATACATGGCGAATACATCATCTGGACACAGGAGTGCGAATGAGCAACTTCCTGCAAGCATGAGCTTTGTGAAGCTGGCTGACATGAATGAGGATGATTGGACCTTGTTTCTAGAGAAGTTTCAAAATCTGCTTTACCCAGCATTTGCAAAACGAAAGTCTGTTACTCTAGGTCAGAGACAGAGACAGAGGCTTGGTACCTCTTGCCAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0030258 GO:0036211 GO:0043170 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0046486 GO:0046488 GO:0046777 GO:0046834 GO:0046854 GO:0052742 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PI3_PI4_kinase

Protein Analysis

636

Amino Acids

70.72

Weight (kDa)

5.61

Isoelectric Point (pI)

55.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI3_PI4_kinase	PF00454	170 - 419	3.7e-44	Phosphatidylinositol 3- and 4-kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13640 AT1G13640 AT1G26270 AT2G03890 AT2G03890
fragaria_vesca	FvH4_4g29190 FvH4_4g29190 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200
malus_domestica	MD13G1055800.v1.1 MD16G1054900.v1.1 MD17G1066800.v1.1
prunus_persica	Prupe.1G295100_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1
pyrus_communis	pycom13g04940 pycom16g04780 pycom17g06720
rosa_chinensis	RchiOBHm_Chr2g0164641 RchiOBHm_Chr4g0437261
rosa_laevigata	RLG00000006453 RLG00000021473
rosa_multiflora	Rmu_sc0004339.1_g000020 Rmu_ssc0000076.1_g000009
rosa_roxburghii	Rroxscaffold_2G00086410 Rroxscaffold_5G00378380
rosa_rugosa	Rorug02G0509500 Rorug04G0298800
rosa_samantha	Rh2AG577400 Rh2BG588600 Rh2CG559400 Rh2DG599200 Rh4AG353700 Rh4BG363500 Rh4CG377700 Rh4DG357600
rosa_wichuraiana	Rw2G047950 Rw4G031030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1892
AccB1I	GGYRCC	2 cut(s)	1360, 1892
AccII	CGCG	2 cut(s)	174, 1424
AccIII	TCCGGA	1 cut(s)	1132
AciI	CCGC	5 cut(s)	62, 174, 652, 1349, 1424
AclWI	GGATC	2 cut(s)	479, 1124
AcsI	RAATTY	1 cut(s)	1280
AcuI	CTGAAG	3 cut(s)	252, 1241, 1655
AfaI	GTAC	3 cut(s)	27, 1055, 1894
AfiI	CCNNNNNNNGG	1 cut(s)	160
AflIII	ACRYGT	1 cut(s)	314
AgsI	TTSAA	5 cut(s)	268, 640, 760, 1438, 1820
AjnI	CCWGG	1 cut(s)	631
AleI	CACNNNNGTG	1 cut(s)	1728
Alw21I	GWGCWC	1 cut(s)	242
Alw26I	GTCTC	5 cut(s)	9, 1193, 1247, 1867, 1873
AlwI	GGATC	2 cut(s)	479, 1124
Ama87I	CYCGRG	1 cut(s)	155
Aor13HI	TCCGGA	1 cut(s)	1132
AoxI	GGCC	4 cut(s)	465, 625, 1021, 1073
ApeKI	GCWGC	9 cut(s)	103, 208, 376, 683, 790, 890, 1153, 1232, 1445
ApoI	RAATTY	1 cut(s)	1280
ArsI	GACNNNNNNTTYG	6 cut(s)	1040, 1072, 1630, 1662, 1838, 1870
Asp700I	GAANNNNTTC	1 cut(s)	295
Asp718I	GGTACC	1 cut(s)	1892
AspS9I	GGNCC	5 cut(s)	395, 625, 1000, 1048, 1797
AsuHPI	GGTGA	8 cut(s)	188, 348, 554, 748, 787, 1274, 1330, 1664
AsuII	TTCGAA	1 cut(s)	289
AvaI	CYCGRG	1 cut(s)	155
AvaII	GGWCC	4 cut(s)	395, 1000, 1048, 1797
AxyI	CCTNAGG	1 cut(s)	1077
BanI	GGYRCC	2 cut(s)	1360, 1892
BanII	GRGCYC	1 cut(s)	242
BbsI	GAAGAC	1 cut(s)	1643
Bbv12I	GWGCWC	1 cut(s)	242
BbvCI	CCTCAGC	1 cut(s)	1389
BbvI	GCAGC	9 cut(s)	115, 220, 388, 695, 777, 902, 1140, 1219, 1457
BccI	CCATC	7 cut(s)	154, 469, 476, 764, 968, 1336, 1615
BceAI	ACGGC	1 cut(s)	1348
BcgI	CGANNNNNNTGC	2 cut(s)	263, 297
BciT130I	CCWGG	1 cut(s)	633
BciVI	GTATCC	2 cut(s)	1059, 1691
BclI	TGATCA	2 cut(s)	862, 1461
BcoDI	GTCTC	5 cut(s)	9, 1193, 1247, 1867, 1873
BfaI	CTAG	4 cut(s)	1275, 1305, 1808, 1865
BfmI	CTRYAG	2 cut(s)	399, 1443
BfuI	GTATCC	2 cut(s)	1059, 1691
BisI	GCNGC	9 cut(s)	104, 209, 377, 684, 791, 891, 1154, 1233, 1446
BlsI	GCNGC	9 cut(s)	105, 210, 378, 685, 792, 892, 1155, 1234, 1447
Bme1390I	CCNGG	1 cut(s)	633
Bme18I	GGWCC	4 cut(s)	395, 1000, 1048, 1797
BmeT110I	CYCGRG	1 cut(s)	155
BmgT120I	GGNCC	5 cut(s)	395, 625, 1000, 1048, 1797
BmiI	GGNNCC	5 cut(s)	953, 1050, 1302, 1362, 1894
BmrFI	CCNGG	1 cut(s)	633
BmrI	ACTGGG	1 cut(s)	372
BmsI	GCATC	4 cut(s)	830, 844, 907, 1140
BmuI	ACTGGG	1 cut(s)	372
BpiI	GAAGAC	1 cut(s)	1643
Bpu10I	CCTNAGC	1 cut(s)	1389
Bpu14I	TTCGAA	1 cut(s)	289
BpuEI	CTTGAG	1 cut(s)	1334
BsaAI	YACGTR	1 cut(s)	317
BsaI	GGTCTC	1 cut(s)	1247
BsaJI	CCNNGG	4 cut(s)	154, 732, 1018, 1371
BsaWI	WCCGGW	1 cut(s)	1132
BsaXI	ACNNNNNCTCC	6 cut(s)	976, 1006, 1037, 1067, 1349, 1379
Bsc4I	CCNNNNNNNGG	1 cut(s)	160
Bse1I	ACTGG	8 cut(s)	69, 148, 367, 670, 858, 884, 1527, 1903
Bse21I	CCTNAGG	1 cut(s)	1077
BseAI	TCCGGA	1 cut(s)	1132
BseBI	CCWGG	1 cut(s)	633
BseDI	CCNNGG	4 cut(s)	154, 732, 1018, 1371
BseGI	GGATG	6 cut(s)	152, 165, 872, 1141, 1347, 1795
BseLI	CCNNNNNNNGG	1 cut(s)	160
BseMII	CTCAG	6 cut(s)	292, 432, 1091, 1110, 1204, 1380
BseNI	ACTGG	8 cut(s)	69, 148, 367, 670, 858, 884, 1527, 1903
BseRI	GAGGAG	6 cut(s)	214, 1310, 1313, 1406, 1500, 1553
BseXI	GCAGC	9 cut(s)	115, 220, 388, 695, 777, 902, 1140, 1219, 1457
BseYI	CCCAGC	1 cut(s)	1834
BsgI	GTGCAG	2 cut(s)	303, 1192
Bsh1236I	CGCG	2 cut(s)	174, 1424
BshFI	GGCC	4 cut(s)	467, 627, 1023, 1075
BshNI	GGYRCC	2 cut(s)	1360, 1892
BsiHKAI	GWGCWC	1 cut(s)	242
BsiHKCI	CYCGRG	1 cut(s)	155
BsiSI	CCGG	1 cut(s)	1133
BslFI	GGGAC	2 cut(s)	883, 1351
BslI	CCNNNNNNNGG	1 cut(s)	160
BsmAI	GTCTC	5 cut(s)	9, 1193, 1247, 1867, 1873
BsmFI	GGGAC	2 cut(s)	883, 1351
BsnI	GGCC	4 cut(s)	467, 627, 1023, 1075
Bso31I	GGTCTC	1 cut(s)	1247
BsoBI	CYCGRG	1 cut(s)	155
Bsp119I	TTCGAA	1 cut(s)	289
Bsp1286I	GDGCHC	1 cut(s)	242
Bsp13I	TCCGGA	1 cut(s)	1132
Bsp143I	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
Bsp19I	CCATGG	1 cut(s)	1018
BspACI	CCGC	5 cut(s)	62, 174, 652, 1349, 1424
BspANI	GGCC	4 cut(s)	467, 627, 1023, 1075
BspCNI	CTCAG	6 cut(s)	291, 431, 1090, 1109, 1203, 1381
BspEI	TCCGGA	1 cut(s)	1132
BspFNI	CGCG	2 cut(s)	174, 1424
BspLI	GGNNCC	5 cut(s)	953, 1050, 1302, 1362, 1894
BspMAI	CTGCAG	1 cut(s)	1447
BspPI	GGATC	2 cut(s)	479, 1124
BspT104I	TTCGAA	1 cut(s)	289
BspT107I	GGYRCC	2 cut(s)	1360, 1892
BspTNI	GGTCTC	1 cut(s)	1247
BsrI	ACTGG	8 cut(s)	69, 148, 367, 670, 858, 884, 1527, 1903
BssECI	CCNNGG	4 cut(s)	154, 732, 1018, 1371
BssMI	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
BssT1I	CCWWGG	3 cut(s)	732, 1018, 1371
Bst2UI	CCWGG	1 cut(s)	633
Bst4CI	ACNGT	1 cut(s)	193
Bst6I	CTCTTC	6 cut(s)	115, 191, 228, 309, 794, 1539
BstBAI	YACGTR	1 cut(s)	317
BstBI	TTCGAA	1 cut(s)	289
BstC8I	GCNNGC	8 cut(s)	62, 66, 108, 213, 945, 1031, 1753, 1773
BstDEI	CTNAG	7 cut(s)	278, 418, 958, 1077, 1096, 1190, 1389
BstDSI	CCRYGG	1 cut(s)	1018
BstF5I	GGATG	6 cut(s)	152, 165, 872, 1141, 1347, 1795
BstFNI	CGCG	2 cut(s)	174, 1424
BstKTI	GATC	9 cut(s)	172, 277, 424, 487, 706, 744, 865, 1132, 1464
BstMAI	GTCTC	5 cut(s)	9, 1193, 1247, 1867, 1873
BstMBI	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
BstMWI	GCNNNNNNNGC	3 cut(s)	505, 649, 1162
BstNI	CCWGG	1 cut(s)	633
BstNSI	RCATGY	2 cut(s)	446, 947
BstSCI	CCNGG	1 cut(s)	631
BstSFI	CTRYAG	2 cut(s)	399, 1443
BstUI	CGCG	2 cut(s)	174, 1424
BstV1I	GCAGC	9 cut(s)	115, 220, 388, 695, 777, 902, 1140, 1219, 1457
BstV2I	GAAGAC	1 cut(s)	1643
BstXI	CCANNNNNNTGG	2 cut(s)	475, 733
Bsu36I	CCTNAGG	1 cut(s)	1077
BsuI	GTATCC	2 cut(s)	1059, 1691
BsuRI	GGCC	4 cut(s)	467, 627, 1023, 1075
BtgI	CCRYGG	1 cut(s)	1018
BtgZI	GCGATG	1 cut(s)	884
BtsCI	GGATG	6 cut(s)	152, 165, 872, 1141, 1347, 1795
BtsI	GCAGTG	2 cut(s)	48, 726
BtsIMutI	CAGTG	6 cut(s)	48, 76, 286, 654, 726, 865
Cac8I	GCNNGC	8 cut(s)	62, 66, 108, 213, 945, 1031, 1753, 1773
Cfr13I	GGNCC	5 cut(s)	395, 625, 1000, 1048, 1797
CseI	GACGC	1 cut(s)	1430
CsiI	ACCWGGT	1 cut(s)	631
Csp6I	GTAC	3 cut(s)	26, 1054, 1893
CspCI	CAANNNNNGTGG	2 cut(s)	426, 461
CviQI	GTAC	3 cut(s)	26, 1054, 1893
DdeI	CTNAG	7 cut(s)	278, 418, 958, 1077, 1096, 1190, 1389
DpnI	GATC	9 cut(s)	171, 276, 423, 486, 705, 743, 864, 1131, 1463
DpnII	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
DraI	TTTAAA	1 cut(s)	930
Eam1104I	CTCTTC	6 cut(s)	115, 191, 228, 309, 794, 1539
EarI	CTCTTC	6 cut(s)	115, 191, 228, 309, 794, 1539
Ecl136II	GAGCTC	1 cut(s)	240
Eco130I	CCWWGG	3 cut(s)	732, 1018, 1371
Eco24I	GRGCYC	1 cut(s)	242
Eco31I	GGTCTC	1 cut(s)	1247
Eco47I	GGWCC	4 cut(s)	395, 1000, 1048, 1797
Eco53kI	GAGCTC	1 cut(s)	240
Eco57I	CTGAAG	3 cut(s)	252, 1241, 1655
Eco81I	CCTNAGG	1 cut(s)	1077
Eco88I	CYCGRG	1 cut(s)	155
EcoICRI	GAGCTC	1 cut(s)	240
EcoO109I	RGGNCCY	1 cut(s)	1048
EcoRII	CCWGG	1 cut(s)	631
EcoT14I	CCWWGG	3 cut(s)	732, 1018, 1371
EcoT38I	GRGCYC	1 cut(s)	242
ErhI	CCWWGG	3 cut(s)	732, 1018, 1371
FaqI	GGGAC	2 cut(s)	883, 1351
FauI	CCCGC	2 cut(s)	69, 645
FbaI	TGATCA	2 cut(s)	862, 1461
Fnu4HI	GCNGC	9 cut(s)	104, 209, 377, 684, 791, 891, 1154, 1233, 1446
FokI	GGATG	6 cut(s)	159, 172, 859, 1148, 1354, 1802
FriOI	GRGCYC	1 cut(s)	242
Fsp4HI	GCNGC	9 cut(s)	104, 209, 377, 684, 791, 891, 1154, 1233, 1446
FspBI	CTAG	4 cut(s)	1275, 1305, 1808, 1865
GluI	GCNGC	9 cut(s)	104, 209, 377, 684, 791, 891, 1154, 1233, 1446
GsaI	CCCAGC	1 cut(s)	1838
HaeIII	GGCC	4 cut(s)	467, 627, 1023, 1075
HapII	CCGG	1 cut(s)	1133
HgaI	GACGC	1 cut(s)	1430
HincII	GTYRAC	1 cut(s)	246
HindII	GTYRAC	1 cut(s)	246
HindIII	AAGCTT	2 cut(s)	1124, 1619
HinfI	GANTC	3 cut(s)	296, 1226, 1427
HpaII	CCGG	1 cut(s)	1133
HphI	GGTGA	8 cut(s)	188, 348, 554, 748, 787, 1274, 1330, 1664
Hpy166II	GTNNAC	4 cut(s)	246, 1000, 1310, 1496
Hpy188I	TCNGA	9 cut(s)	117, 421, 674, 1011, 1099, 1221, 1432, 1597, 1872
Hpy188III	TCNNGA	7 cut(s)	157, 472, 1133, 1465, 1666, 1719, 1808
Hpy8I	GTNNAC	4 cut(s)	246, 1000, 1310, 1496
HpyAV	CCTTC	5 cut(s)	457, 1152, 1216, 1550, 1602
HpyCH4III	ACNGT	1 cut(s)	193
HpyCH4IV	ACGT	1 cut(s)	316
HpyF10VI	GCNNNNNNNGC	3 cut(s)	505, 649, 1162
HpyF3I	CTNAG	7 cut(s)	278, 418, 958, 1077, 1096, 1190, 1389
HpySE526I	ACGT	1 cut(s)	316
Kpn2I	TCCGGA	1 cut(s)	1132
KpnI	GGTACC	1 cut(s)	1896
Ksp22I	TGATCA	2 cut(s)	862, 1461
Kzo9I	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
LmnI	GCTCC	5 cut(s)	81, 1162, 1300, 1385, 1679
Lsp1109I	GCAGC	9 cut(s)	115, 220, 388, 695, 777, 902, 1140, 1219, 1457
LweI	GCATC	4 cut(s)	830, 844, 907, 1140
MabI	ACCWGGT	1 cut(s)	631
MaeI	CTAG	4 cut(s)	1275, 1305, 1808, 1865
MaeII	ACGT	1 cut(s)	316
MaeIII	GTNAC	3 cut(s)	176, 317, 1858
MalI	GATC	9 cut(s)	171, 276, 423, 486, 705, 743, 864, 1131, 1463
MboI	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
MhlI	GDGCHC	1 cut(s)	242
MluCI	AATT	3 cut(s)	164, 489, 1280
MlyI	GAGTC	1 cut(s)	1235
MmeI	TCCRAC	2 cut(s)	95, 251
MroI	TCCGGA	1 cut(s)	1132
MroXI	GAANNNNTTC	1 cut(s)	295
MseI	TTAA	3 cut(s)	54, 459, 929
MslI	CAYNNNNRTG	2 cut(s)	473, 1728
MspA1I	CMGCKG	1 cut(s)	893
MspI	CCGG	1 cut(s)	1133
MspR9I	CCNGG	1 cut(s)	633
MvaI	CCWGG	1 cut(s)	633
MvnI	CGCG	2 cut(s)	174, 1424
MwoI	GCNNNNNNNGC	3 cut(s)	505, 649, 1162
NcoI	CCATGG	1 cut(s)	1018
NdeII	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
NlaIV	GGNNCC	5 cut(s)	953, 1050, 1302, 1362, 1894
NmuCI	GTSAC	1 cut(s)	176
NspI	RCATGY	2 cut(s)	446, 947
NspV	TTCGAA	1 cut(s)	289
OliI	CACNNNNGTG	1 cut(s)	1728
PaeI	GCATGC	1 cut(s)	947
PdmI	GAANNNNTTC	1 cut(s)	295
PfeI	GAWTC	2 cut(s)	296, 1427
PflFI	GACNNNGTC	1 cut(s)	1500
PkrI	GCNGC	9 cut(s)	105, 210, 378, 685, 792, 892, 1155, 1234, 1447
PleI	GAGTC	1 cut(s)	1234
PpsI	GAGTC	1 cut(s)	1234
Ppu21I	YACGTR	1 cut(s)	317
PpuMI	RGGWCCY	1 cut(s)	1048
Psp124BI	GAGCTC	1 cut(s)	242
Psp5II	RGGWCCY	1 cut(s)	1048
Psp6I	CCWGG	1 cut(s)	631
PspFI	CCCAGC	1 cut(s)	1834
PspGI	CCWGG	1 cut(s)	631
PspN4I	GGNNCC	5 cut(s)	953, 1050, 1302, 1362, 1894
PspPI	GGNCC	5 cut(s)	395, 625, 1000, 1048, 1797
PspPPI	RGGWCCY	1 cut(s)	1048
PstI	CTGCAG	1 cut(s)	1447
PsyI	GACNNNGTC	1 cut(s)	1500
PvuII	CAGCTG	1 cut(s)	893
RsaI	GTAC	3 cut(s)	27, 1055, 1894
RsaNI	GTAC	3 cut(s)	26, 1054, 1893
RseI	CAYNNNNRTG	2 cut(s)	473, 1728
SacI	GAGCTC	1 cut(s)	242
SaqAI	TTAA	3 cut(s)	54, 459, 929
SatI	GCNGC	9 cut(s)	104, 209, 377, 684, 791, 891, 1154, 1233, 1446
Sau3AI	GATC	9 cut(s)	169, 274, 421, 484, 703, 741, 862, 1129, 1461
Sau96I	GGNCC	5 cut(s)	395, 625, 1000, 1048, 1797
SchI	GAGTC	1 cut(s)	1235
ScrFI	CCNGG	1 cut(s)	633
SduI	GDGCHC	1 cut(s)	242
SexAI	ACCWGGT	1 cut(s)	631
SfaNI	GCATC	4 cut(s)	830, 844, 907, 1140
SfcI	CTRYAG	2 cut(s)	399, 1443
SfuI	TTCGAA	1 cut(s)	289
SinI	GGWCC	4 cut(s)	395, 1000, 1048, 1797
SmiMI	CAYNNNNRTG	2 cut(s)	473, 1728
SmlI	CTYRAG	1 cut(s)	1313
SmoI	CTYRAG	1 cut(s)	1313
SphI	GCATGC	1 cut(s)	947
Sse9I	AATT	3 cut(s)	164, 489, 1280
SsiI	CCGC	5 cut(s)	62, 174, 652, 1349, 1424
SspMI	CTAG	4 cut(s)	1275, 1305, 1808, 1865
SstI	GAGCTC	1 cut(s)	242
StyD4I	CCNGG	1 cut(s)	631
StyI	CCWWGG	3 cut(s)	732, 1018, 1371
TaaI	ACNGT	1 cut(s)	193
TaiI	ACGT	1 cut(s)	319
TaqI	TCGA	3 cut(s)	289, 433, 696
TasI	AATT	3 cut(s)	164, 489, 1280
TfiI	GAWTC	2 cut(s)	296, 1427
Tru1I	TTAA	3 cut(s)	54, 459, 929
Tru9I	TTAA	3 cut(s)	54, 459, 929
TscAI	CASTG	6 cut(s)	48, 76, 286, 654, 733, 865
TseFI	GTSAC	1 cut(s)	176
TseI	GCWGC	9 cut(s)	103, 208, 376, 683, 790, 890, 1153, 1232, 1445
Tsp45I	GTSAC	1 cut(s)	176
TspDTI	ATGAA	2 cut(s)	588, 1796
TspRI	CASTG	6 cut(s)	48, 76, 286, 654, 733, 865
Tth111I	GACNNNGTC	1 cut(s)	1500
VpaK11BI	GGWCC	4 cut(s)	395, 1000, 1048, 1797
XapI	RAATTY	1 cut(s)	1280
XbaI	TCTAGA	1 cut(s)	1807
XceI	RCATGY	2 cut(s)	446, 947
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1378
XmnI	GAANNNNTTC	1 cut(s)	295
XspI	CTAG	4 cut(s)	1275, 1305, 1808, 1865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.