Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001564

Physical Location & Seq

Genomic Coordinates Reverse (-)

3876653 .. 3880081

Gene Structure 3429 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1054900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1926 bp

ATGTCTCGTAAGCTGGACAGCCCTGTACAGACCCAGATGCCAGCGGCACTATTTAGCAATCCGCTCAGTGGGGAGTACCATGGAAGCAAGAGAACGGAAGGGAAGCAGCACACTGGTAGGAGGCGAGTTTTTGTGCAAACTGAAAGTGGATGTGTCTTGGGGATGGATTTGGATCGCAGCGATAGTGTCCATACTGTGAAGAGAAGATTGCAGATTGCTCTCAATGTTCCAACTGATGAAAGCTCGTTGACTTTTGGGGATGTGGTGTTGAAGAATGATCTGAGTGCGGTTAGGAATGATTCCCCTCTTCTTCTCACCAGGAACCTTATGCATAGAAGTTCATCAACTCCATGTCTCTCACCTACTGGAAGGGATCTGCAGCAGAGAGATCGGAGTGGTCCTATAGAGACATTAGGGAACTCAGATCGGTTTGCTAGGACAAAACAAGTGGTCAAGGATATGGTGAAGGCCATCAAGATGGGGGTTGATCCAATTCCTGTTCATAGCGGGCTTGGAGGTGCATACTATTTCAGGAACAGCAGAGGTGAGTGTGTTTCGATAGTGAAGCCAACTGATGAAGAACCTTTTGCGCCTAACAACCCAAAAGGTTTTGTCGGTAAAGCTCTTGGGCAACCAGGTTTGAAGCGCTCGGTGCGAGTTGGGGAGACTGGATTCAGAGAAGTCGCAGCTTTCCTGCTTGACAAAGATCACTTTGCCAATGTGCCTCCCACTGCCTTGGTCAAGATCACTCACTCTATCTTTAACATAAACGATGGGGTGAACGGGAATAAGACTCCTCAGAAGAAGCTGGTTAGCAAGATTGCATCTTGCCAGCAATTCATCCAACATGACTTTGATGCGAGTGATCACGGGACATCCAGCTTCCCCGTAACTTCTGTGCATCGTATAGGGATATTAGACATTAGGATTTTTAACACAGACAGGCATGCAGGGAACCTTTTAGTTAGGAAACTGGATGGTGTCGGGATGTTTGGTCAAGTGGAGCTCATTCCAATTGACCATGGCCTTTGTTTGCCAGAAACTTTGGAGGACCCCTACTTTGAGTGGATTCATTGGCCTCAGGCTTCCATTCCATTCACAGATGACGAGCTTGAGTACATAGAAAAACTTGATCCACTTGAGGATTGTGAGATGTTGCGCAGGGAGCTTCCCATGATGCGAGAGGCTTGTCTCAGGGTTTTGGTTCTTTGCACCATTTTCCTGAAGGAGTCTGCTGCATATGGTCTCTGTCTTGCTGAAATAGGTGAGATGATGACTAGGGAATTTCGCAGTGGCGAGGAGGAACCTAGTGAACTTGAGATAATTTGTATGGAGGCGAGGAGGATGTTGGCAGAGAGGGAGGAGGTGCTGTCTCCCAAGGCTGATTCGGGAGATCAGGAGTTCCTGTTTGACATAGACTGTGAGGACGCGGAGGAAGACTACACCTCCAAATTTGCAGCAGATGATTATCTGAACAGGTCATCATCCTTTCCATTTGCAAATGGGAGTGGGCATGGCCGCTCGCTTCTCTCTAAACTTGAAGAAAGCATCGAGGAGGAAGAGGACAGTGAAGTGGAAGAACAAGAGGCATTGGCATATATGCCAACTCCAGAAAAGGTCCCAAGCGTTTCAAAACTTTCTATGTCGCTGAAGAATACAGTTTTGGGAGAGAAGAGCCACCTGAAGTATAAGGGTTCCAAACCAGAAAATGGGTACATGGCGAATACATCATTGGGGCACAGGAGTGCAAACGAGCAGCTTCCTGCAAGCATGAGCTTTGTGAAGCTGGCTGACATGAGCGAGGATGAGTGGACCTTGTTTCTGGAGAAGTTTCAAGATCTGCTGTACCCGGCATTTGCCAAACGCAAATCTGTTACCCTAGGTCAGAGGCAGAGACAGAGGCTCGGCACTTCGTGCCAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0030258 GO:0036211 GO:0043170 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0046486 GO:0046488 GO:0046777 GO:0046834 GO:0046854 GO:0052742 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PI3_PI4_kinase

Protein Analysis

642

Amino Acids

71.73

Weight (kDa)

5.45

Isoelectric Point (pI)

57.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI3_PI4_kinase	PF00454	172 - 420	2.9e-46	Phosphatidylinositol 3- and 4-kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13640 AT1G13640 AT1G26270 AT2G03890 AT2G03890
fragaria_vesca	FvH4_4g29190 FvH4_4g29190 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200 FvH4_6g46200
malus_domestica	MD13G1055800.v1.1 MD16G1054900.v1.1 MD17G1066800.v1.1
prunus_persica	Prupe.1G295100_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1 Prupe.3G247900_v2.0.a1
pyrus_communis	pycom13g04940 pycom16g04780 pycom17g06720
rosa_chinensis	RchiOBHm_Chr2g0164641 RchiOBHm_Chr4g0437261
rosa_laevigata	RLG00000006453 RLG00000021473
rosa_multiflora	Rmu_sc0004339.1_g000020 Rmu_ssc0000076.1_g000009
rosa_roxburghii	Rroxscaffold_2G00086410 Rroxscaffold_5G00378380
rosa_rugosa	Rorug02G0509500 Rorug04G0298800
rosa_samantha	Rh2AG577400 Rh2BG588600 Rh2CG559400 Rh2DG599200 Rh4AG353700 Rh4BG363500 Rh4CG377700 Rh4DG357600
rosa_wichuraiana	Rw2G047950 Rw4G031030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1160
AccB7I	CCANNNNNTGG	1 cut(s)	1709
AccBSI	CCGCTC	2 cut(s)	64, 1521
AccII	CGCG	1 cut(s)	1430
AciI	CCGC	6 cut(s)	44, 62, 287, 507, 1430, 1519
AclWI	GGATC	4 cut(s)	180, 381, 482, 1127
AcoI	YGGCCR	1 cut(s)	1516
AcsI	RAATTY	2 cut(s)	1283, 1451
AcuI	CTGAAG	3 cut(s)	1244, 1670, 1703
AdeI	CACNNNGTG	1 cut(s)	1914
AfaI	GTAC	5 cut(s)	27, 77, 1118, 1715, 1847
AfeI	AGCGCT	1 cut(s)	647
AfiI	CCNNNNNNNGG	2 cut(s)	68, 1709
AgsI	TTSAA	5 cut(s)	271, 643, 1541, 1632, 1835
AjnI	CCWGG	2 cut(s)	317, 634
AjuI	GAANNNNNNNTTGG	4 cut(s)	1646, 1678, 1715, 1747
AleI	CACNNNNGTG	1 cut(s)	1743
Alw21I	GWGCWC	1 cut(s)	1008
Alw26I	GTCTC	8 cut(s)	9, 359, 401, 659, 1196, 1250, 1377, 1888
AlwI	GGATC	4 cut(s)	180, 381, 482, 1127
Aor51HI	AGCGCT	1 cut(s)	647
AoxI	GGCC	4 cut(s)	468, 1024, 1076, 1516
ApeKI	GCWGC	7 cut(s)	106, 177, 379, 686, 1235, 1457, 1756
ApoI	RAATTY	2 cut(s)	1283, 1451
ArsI	GACNNNNNNTTYG	2 cut(s)	1043, 1075
AspA2I	CCTAGG	1 cut(s)	1879
AspLEI	GCGC	3 cut(s)	592, 648, 1161
AspS9I	GGNCC	4 cut(s)	398, 1051, 1618, 1812
AsuC2I	CCSGG	1 cut(s)	1850
AsuHPI	GGTGA	6 cut(s)	307, 351, 475, 557, 790, 1277
AvaII	GGWCC	4 cut(s)	398, 1051, 1618, 1812
AvrII	CCTAGG	1 cut(s)	1879
AxyI	CCTNAGG	1 cut(s)	1080
BaeGI	GKGCMC	1 cut(s)	1740
BanII	GRGCYC	1 cut(s)	1008
BbsI	GAAGAC	1 cut(s)	1443
Bbv12I	GWGCWC	1 cut(s)	1008
BbvI	GCAGC	7 cut(s)	118, 189, 391, 698, 1222, 1469, 1768
BccI	CCATC	5 cut(s)	157, 472, 479, 767, 971
BcgI	CGANNNNNNTGC	2 cut(s)	371, 405
BciT130I	CCWGG	2 cut(s)	319, 636
BclI	TGATCA	1 cut(s)	865
BcnI	CCSGG	1 cut(s)	1850
BcoDI	GTCTC	8 cut(s)	9, 359, 401, 659, 1196, 1250, 1377, 1888
BfaI	CTAG	4 cut(s)	435, 1278, 1308, 1880
BfmI	CTRYAG	2 cut(s)	377, 402
BfoI	RGCGCY	1 cut(s)	649
BglII	AGATCT	1 cut(s)	1837
BisI	GCNGC	9 cut(s)	45, 107, 178, 380, 687, 1236, 1458, 1519, 1757
BlnI	CCTAGG	1 cut(s)	1879
BlsI	GCNGC	9 cut(s)	46, 108, 179, 381, 688, 1237, 1459, 1520, 1758
Bme1390I	CCNGG	3 cut(s)	319, 636, 1850
Bme18I	GGWCC	4 cut(s)	398, 1051, 1618, 1812
BmgT120I	GGNCC	4 cut(s)	398, 1051, 1618, 1812
BmiI	GGNNCC	6 cut(s)	323, 956, 1053, 1305, 1620, 1696
BmrFI	CCNGG	3 cut(s)	319, 636, 1850
BmsI	GCATC	6 cut(s)	27, 833, 847, 910, 1167, 1557
BpiI	GAAGAC	1 cut(s)	1443
BpmI	CTGGAG	2 cut(s)	1593, 1844
BpuEI	CTTGAG	3 cut(s)	1133, 1160, 1337
BpuMI	CCSGG	1 cut(s)	1850
BsaBI	GATNNNNATC	2 cut(s)	171, 1467
BsaI	GGTCTC	1 cut(s)	1250
BsaJI	CCNNGG	5 cut(s)	79, 735, 1021, 1377, 1879
BsaXI	ACNNNNNCTCC	6 cut(s)	1355, 1385, 1391, 1421, 1430, 1460
Bsc4I	CCNNNNNNNGG	2 cut(s)	68, 1709
Bse1I	ACTGG	5 cut(s)	118, 370, 673, 978, 1918
Bse21I	CCTNAGG	1 cut(s)	1080
Bse8I	GATNNNNATC	2 cut(s)	171, 1467
BseBI	CCWGG	2 cut(s)	319, 636
BseDI	CCNNGG	5 cut(s)	79, 735, 1021, 1377, 1879
BseJI	GATNNNNATC	2 cut(s)	171, 1467
BseLI	CCNNNNNNNGG	2 cut(s)	68, 1709
BseMII	CTCAG	6 cut(s)	79, 272, 435, 812, 1094, 1207
BseNI	ACTGG	5 cut(s)	118, 370, 673, 978, 1918
BseRI	GAGGAG	5 cut(s)	786, 1313, 1354, 1376, 1568
BseSI	GKGCMC	1 cut(s)	1740
BseXI	GCAGC	7 cut(s)	118, 189, 391, 698, 1222, 1469, 1768
Bsh1236I	CGCG	1 cut(s)	1430
BshFI	GGCC	4 cut(s)	470, 1026, 1078, 1518
BsiHKAI	GWGCWC	1 cut(s)	1008
BsiSI	CCGG	1 cut(s)	1850
BslFI	GGGAC	2 cut(s)	886, 1604
BslI	CCNNNNNNNGG	2 cut(s)	68, 1709
BsmAI	GTCTC	8 cut(s)	9, 359, 401, 659, 1196, 1250, 1377, 1888
BsmFI	GGGAC	2 cut(s)	886, 1604
BsnI	GGCC	4 cut(s)	470, 1026, 1078, 1518
Bso31I	GGTCTC	1 cut(s)	1250
Bsp1286I	GDGCHC	2 cut(s)	1008, 1740
Bsp1407I	TGTACA	1 cut(s)	25
Bsp19I	CCATGG	2 cut(s)	79, 1021
BspACI	CCGC	6 cut(s)	44, 62, 287, 507, 1430, 1519
BspANI	GGCC	4 cut(s)	470, 1026, 1078, 1518
BspCNI	CTCAG	6 cut(s)	78, 273, 434, 811, 1093, 1206
BspFNI	CGCG	1 cut(s)	1430
BspLI	GGNNCC	6 cut(s)	323, 956, 1053, 1305, 1620, 1696
BspMAI	CTGCAG	1 cut(s)	381
BspPI	GGATC	4 cut(s)	180, 381, 482, 1127
BspQI	GCTCTTC	1 cut(s)	1667
BspTNI	GGTCTC	1 cut(s)	1250
BsrBI	CCGCTC	2 cut(s)	64, 1521
BsrGI	TGTACA	1 cut(s)	25
BsrI	ACTGG	5 cut(s)	118, 370, 673, 978, 1918
BssECI	CCNNGG	5 cut(s)	79, 735, 1021, 1377, 1879
BssT1I	CCWWGG	5 cut(s)	79, 735, 1021, 1377, 1879
Bst2UI	CCWGG	2 cut(s)	319, 636
Bst4CI	ACNGT	4 cut(s)	196, 1421, 1568, 1660
Bst6I	CTCTTC	4 cut(s)	194, 312, 1554, 1667
BstAPI	GCANNNNNTGC	1 cut(s)	1914
BstAUI	TGTACA	1 cut(s)	25
BstC8I	GCNNGC	7 cut(s)	42, 509, 833, 948, 1523, 1768, 1788
BstDEI	CTNAG	6 cut(s)	65, 281, 421, 798, 1080, 1193
BstDSI	CCRYGG	2 cut(s)	79, 1021
BstFNI	CGCG	1 cut(s)	1430
BstH2I	RGCGCY	1 cut(s)	649
BstHHI	GCGC	3 cut(s)	592, 648, 1161
BstMAI	GTCTC	8 cut(s)	9, 359, 401, 659, 1196, 1250, 1377, 1888
BstMWI	GCNNNNNNNGC	3 cut(s)	652, 1165, 1914
BstNI	CCWGG	2 cut(s)	319, 636
BstNSI	RCATGY	1 cut(s)	950
BstSCI	CCNGG	3 cut(s)	317, 634, 1848
BstSFI	CTRYAG	2 cut(s)	377, 402
BstSLI	GKGCMC	1 cut(s)	1740
BstUI	CGCG	1 cut(s)	1430
BstV1I	GCAGC	7 cut(s)	118, 189, 391, 698, 1222, 1469, 1768
BstV2I	GAAGAC	1 cut(s)	1443
BstX2I	RGATCY	2 cut(s)	373, 1837
BstXI	CCANNNNNNTGG	2 cut(s)	478, 736
BstYI	RGATCY	2 cut(s)	373, 1837
Bsu36I	CCTNAGG	1 cut(s)	1080
BsuRI	GGCC	4 cut(s)	470, 1026, 1078, 1518
BtgI	CCRYGG	2 cut(s)	79, 1021
BtsI	GCAGTG	2 cut(s)	729, 1297
BtsIMutI	CAGTG	5 cut(s)	73, 111, 729, 1297, 1573
Cac8I	GCNNGC	7 cut(s)	42, 509, 833, 948, 1523, 1768, 1788
CfoI	GCGC	3 cut(s)	592, 648, 1161
Cfr13I	GGNCC	4 cut(s)	398, 1051, 1618, 1812
CseI	GACGC	1 cut(s)	1436
CsiI	ACCWGGT	1 cut(s)	634
Csp6I	GTAC	5 cut(s)	26, 76, 1117, 1714, 1846
CspCI	CAANNNNNGTGG	2 cut(s)	429, 464
CviQI	GTAC	5 cut(s)	26, 76, 1117, 1714, 1846
DdeI	CTNAG	6 cut(s)	65, 281, 421, 798, 1080, 1193
DraIII	CACNNNGTG	1 cut(s)	1914
EaeI	YGGCCR	1 cut(s)	1516
Eam1104I	CTCTTC	4 cut(s)	194, 312, 1554, 1667
EarI	CTCTTC	4 cut(s)	194, 312, 1554, 1667
Ecl136II	GAGCTC	1 cut(s)	1006
Eco130I	CCWWGG	5 cut(s)	79, 735, 1021, 1377, 1879
Eco24I	GRGCYC	1 cut(s)	1008
Eco31I	GGTCTC	1 cut(s)	1250
Eco47I	GGWCC	4 cut(s)	398, 1051, 1618, 1812
Eco47III	AGCGCT	1 cut(s)	647
Eco53kI	GAGCTC	1 cut(s)	1006
Eco57I	CTGAAG	3 cut(s)	1244, 1670, 1703
Eco81I	CCTNAGG	1 cut(s)	1080
EcoICRI	GAGCTC	1 cut(s)	1006
EcoO109I	RGGNCCY	2 cut(s)	1051, 1618
EcoRII	CCWGG	2 cut(s)	317, 634
EcoT14I	CCWWGG	5 cut(s)	79, 735, 1021, 1377, 1879
EcoT22I	ATGCAT	1 cut(s)	333
EcoT38I	GRGCYC	1 cut(s)	1008
ErhI	CCWWGG	5 cut(s)	79, 735, 1021, 1377, 1879
FaqI	GGGAC	2 cut(s)	886, 1604
FauI	CCCGC	1 cut(s)	500
FauNDI	CATATG	1 cut(s)	1240
FbaI	TGATCA	1 cut(s)	865
Fnu4HI	GCNGC	9 cut(s)	45, 107, 178, 380, 687, 1236, 1458, 1519, 1757
FriOI	GRGCYC	1 cut(s)	1008
Fsp4HI	GCNGC	9 cut(s)	45, 107, 178, 380, 687, 1236, 1458, 1519, 1757
FspBI	CTAG	4 cut(s)	435, 1278, 1308, 1880
FspI	TGCGCA	1 cut(s)	1160
GlaI	GCGC	3 cut(s)	591, 647, 1160
GluI	GCNGC	9 cut(s)	45, 107, 178, 380, 687, 1236, 1458, 1519, 1757
GsuI	CTGGAG	2 cut(s)	1593, 1844
HaeII	RGCGCY	1 cut(s)	649
HaeIII	GGCC	4 cut(s)	470, 1026, 1078, 1518
HapII	CCGG	1 cut(s)	1850
HgaI	GACGC	1 cut(s)	1436
HhaI	GCGC	3 cut(s)	592, 648, 1161
Hin6I	GCGC	3 cut(s)	590, 646, 1159
HinP1I	GCGC	3 cut(s)	590, 646, 1159
HincII	GTYRAC	1 cut(s)	249
HindII	GTYRAC	1 cut(s)	249
HinfI	GANTC	6 cut(s)	299, 672, 793, 1069, 1229, 1385
HpaII	CCGG	1 cut(s)	1850
HphI	GGTGA	6 cut(s)	307, 351, 475, 557, 790, 1277
Hpy166II	GTNNAC	4 cut(s)	249, 781, 1313, 1812
Hpy188I	TCNGA	7 cut(s)	282, 393, 424, 677, 801, 1473, 1887
Hpy8I	GTNNAC	4 cut(s)	249, 781, 1313, 1812
HpyAV	CCTTC	4 cut(s)	92, 363, 460, 1219
HpyCH4III	ACNGT	4 cut(s)	196, 1421, 1568, 1660
HpyF10VI	GCNNNNNNNGC	3 cut(s)	652, 1165, 1914
HpyF3I	CTNAG	6 cut(s)	65, 281, 421, 798, 1080, 1193
HspAI	GCGC	3 cut(s)	590, 646, 1159
Ksp22I	TGATCA	1 cut(s)	865
LguI	GCTCTTC	1 cut(s)	1667
LmnI	GCTCC	2 cut(s)	1003, 1165
Lsp1109I	GCAGC	7 cut(s)	118, 189, 391, 698, 1222, 1469, 1768
LweI	GCATC	6 cut(s)	27, 833, 847, 910, 1167, 1557
MabI	ACCWGGT	1 cut(s)	634
MaeI	CTAG	4 cut(s)	435, 1278, 1308, 1880
MaeIII	GTNAC	2 cut(s)	889, 1873
MbiI	CCGCTC	2 cut(s)	64, 1521
MfeI	CAATTG	1 cut(s)	1014
MflI	RGATCY	2 cut(s)	373, 1837
MhlI	GDGCHC	2 cut(s)	1008, 1740
MluCI	AATT	6 cut(s)	492, 836, 1014, 1283, 1323, 1451
MlyI	GAGTC	2 cut(s)	787, 1238
MmeI	TCCRAC	2 cut(s)	254, 868
Mph1103I	ATGCAT	1 cut(s)	333
MseI	TTAA	2 cut(s)	762, 933
MslI	CAYNNNNRTG	2 cut(s)	476, 1743
MspA1I	CMGCKG	1 cut(s)	44
MspI	CCGG	1 cut(s)	1850
MspR9I	CCNGG	3 cut(s)	319, 636, 1850
MunI	CAATTG	1 cut(s)	1014
MvaI	CCWGG	2 cut(s)	319, 636
MvnI	CGCG	1 cut(s)	1430
MwoI	GCNNNNNNNGC	3 cut(s)	652, 1165, 1914
NciI	CCSGG	1 cut(s)	1850
NcoI	CCATGG	2 cut(s)	79, 1021
NdeI	CATATG	1 cut(s)	1240
NlaIV	GGNNCC	6 cut(s)	323, 956, 1053, 1305, 1620, 1696
NmeAIII	GCCGAG	1 cut(s)	1884
NsbI	TGCGCA	1 cut(s)	1160
NsiI	ATGCAT	1 cut(s)	333
NspI	RCATGY	1 cut(s)	950
OliI	CACNNNNGTG	1 cut(s)	1743
PaeI	GCATGC	1 cut(s)	950
PciSI	GCTCTTC	1 cut(s)	1667
PfeI	GAWTC	4 cut(s)	299, 672, 1069, 1385
PflMI	CCANNNNNTGG	1 cut(s)	1709
PkrI	GCNGC	9 cut(s)	46, 108, 179, 381, 688, 1237, 1459, 1520, 1758
PleI	GAGTC	2 cut(s)	787, 1237
PpsI	GAGTC	2 cut(s)	787, 1237
PpuMI	RGGWCCY	2 cut(s)	1051, 1618
Psp124BI	GAGCTC	1 cut(s)	1008
Psp5II	RGGWCCY	2 cut(s)	1051, 1618
Psp6I	CCWGG	2 cut(s)	317, 634
PspGI	CCWGG	2 cut(s)	317, 634
PspN4I	GGNNCC	6 cut(s)	323, 956, 1053, 1305, 1620, 1696
PspPI	GGNCC	4 cut(s)	398, 1051, 1618, 1812
PspPPI	RGGWCCY	2 cut(s)	1051, 1618
PstI	CTGCAG	1 cut(s)	381
PsuI	RGATCY	2 cut(s)	373, 1837
RsaI	GTAC	5 cut(s)	27, 77, 1118, 1715, 1847
RsaNI	GTAC	5 cut(s)	26, 76, 1117, 1714, 1846
RseI	CAYNNNNRTG	2 cut(s)	476, 1743
SacI	GAGCTC	1 cut(s)	1008
SapI	GCTCTTC	1 cut(s)	1667
SaqAI	TTAA	2 cut(s)	762, 933
SatI	GCNGC	9 cut(s)	45, 107, 178, 380, 687, 1236, 1458, 1519, 1757
Sau96I	GGNCC	4 cut(s)	398, 1051, 1618, 1812
SchI	GAGTC	2 cut(s)	787, 1238
ScrFI	CCNGG	3 cut(s)	319, 636, 1850
SduI	GDGCHC	2 cut(s)	1008, 1740
SexAI	ACCWGGT	1 cut(s)	634
SfaNI	GCATC	6 cut(s)	27, 833, 847, 910, 1167, 1557
SfcI	CTRYAG	2 cut(s)	377, 402
SinI	GGWCC	4 cut(s)	398, 1051, 1618, 1812
SmiMI	CAYNNNNRTG	2 cut(s)	476, 1743
SmlI	CTYRAG	3 cut(s)	1112, 1139, 1316
SmoI	CTYRAG	3 cut(s)	1112, 1139, 1316
SphI	GCATGC	1 cut(s)	950
Sse9I	AATT	6 cut(s)	492, 836, 1014, 1283, 1323, 1451
SsiI	CCGC	6 cut(s)	44, 62, 287, 507, 1430, 1519
SspMI	CTAG	4 cut(s)	435, 1278, 1308, 1880
SstI	GAGCTC	1 cut(s)	1008
StyD4I	CCNGG	3 cut(s)	317, 634, 1848
StyI	CCWWGG	5 cut(s)	79, 735, 1021, 1377, 1879
TaaI	ACNGT	4 cut(s)	196, 1421, 1568, 1660
TaqI	TCGA	2 cut(s)	557, 1551
TasI	AATT	6 cut(s)	492, 836, 1014, 1283, 1323, 1451
TatI	WGTACW	2 cut(s)	25, 1116
TauI	GCSGC	2 cut(s)	47, 1521
TfiI	GAWTC	4 cut(s)	299, 672, 1069, 1385
Tru1I	TTAA	2 cut(s)	762, 933
Tru9I	TTAA	2 cut(s)	762, 933
TscAI	CASTG	5 cut(s)	73, 118, 736, 1297, 1573
TseI	GCWGC	7 cut(s)	106, 177, 379, 686, 1235, 1457, 1756
TspDTI	ATGAA	6 cut(s)	252, 330, 491, 591, 829, 1061
TspGWI	ACGGA	1 cut(s)	110
TspRI	CASTG	5 cut(s)	73, 118, 736, 1297, 1573
Van91I	CCANNNNNTGG	1 cut(s)	1709
VpaK11BI	GGWCC	4 cut(s)	398, 1051, 1618, 1812
XapI	RAATTY	2 cut(s)	1283, 1451
XceI	RCATGY	1 cut(s)	950
XmaJI	CCTAGG	1 cut(s)	1879
XspI	CTAG	4 cut(s)	435, 1278, 1308, 1880
Zsp2I	ATGCAT	1 cut(s)	333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.