MD13G1060200.v1.1

6-phosphogluconolactonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006227
Physical Location & Seq
Reverse (-)
4220152 .. 4223324
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1060200.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCACTTTCCGGTGTTCGGAAAGATAGAGAATTGAGGATTCATGAAAGTTTGGATGATCTTAGCACTGATTTGGCAGACTACATTGCTGAGATATCAGAGGCATCCGTGAAGGAGCGCGGCGTCTTTACAATTGCATTATCTGGTGGTTCTCTCATTGGCTTAATGGGAAAACTCTGTGAAGCTCCTTATAACAAGACTGTAGATTGGGCCAAATGGTATATATTTTGGGCAGACGAGCGTGTTGTGGCGAAAAGTCATGTTGATAGCAATTACAAGCTTGCGAAAGATCGCTTTTTGTCTAAGGTACCTATAATTCCCAGTCATGTGCATTCTATCAATGATTCAGTGTCAGCAGAGGAGGCTGCTGATGACTACGGGTTTGTCATTCGCCAGCTAGTAAAATCTCGTGTGATCAGCGTGTCTGATATAAGTGACTGTCCCAAGTTTGACCTAATCCTCCTGGGAATGGGTCCTGATGGGCACGTCGCCTCGCTATTCCCCAACCACTCGGTGCTGGAGGAGAAAGATGAATGGGTAAGTTTTATAACTGATTCCCCCAAGCCTCCACCTGAGAGAATCACATTCACCCTGCCGGTCATCAACTCGGCATCCAATGTAGCTATAGTCGCAACAGGTGAAAGCAAAGCAGAGGCTGCGCACCTGGCAGTCGATGTCATGGGATCTGACCTCCCTCCAGTGCCTGCTGGAGTTGTCCAGCCTCTGAAAGGGAAGCTGGTGTGGTTTTTGGACAAGGCAGCTGCCTCAAAACTTGATGCCAGTACTCAATTTTCCGCATAG

Protein Analysis

267

Amino Acids

28.9

Weight (kDa)

5.17

Isoelectric Point (pI)

24.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucosamine_iso PF01182 18 - 248 1.9e-72 Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 192, 548
Acc16I TGCGCA 1 cut(s) 660
Acc65I GGTACC 1 cut(s) 307
AccB1I GGYRCC 1 cut(s) 307
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 120, 795
AclWI GGATC 1 cut(s) 691
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 514
AfaI GTAC 2 cut(s) 309, 784
AfiI CCNNNNNNNGG 3 cut(s) 18, 469, 728
AjiI CACGTC 1 cut(s) 487
AjnI CCWGG 2 cut(s) 462, 663
AluBI AGCT 6 cut(s) 185, 280, 397, 623, 736, 761
AluI AGCT 6 cut(s) 185, 280, 397, 623, 736, 761
AlwI GGATC 1 cut(s) 691
AlwNI CAGNNNCTG 3 cut(s) 656, 704, 724
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 4 cut(s) 365, 656, 758, 761
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
Asp718I GGTACC 1 cut(s) 307
AspLEI GCGC 2 cut(s) 120, 661
AspS9I GGNCC 2 cut(s) 210, 473
AsuHPI GGTGA 2 cut(s) 580, 650
AvaII GGWCC 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 486
BanI GGYRCC 1 cut(s) 307
BauI CACGAG 1 cut(s) 408
BbvI GCAGC 4 cut(s) 352, 643, 748, 770
BccI CCATC 1 cut(s) 473
BciT130I CCWGG 2 cut(s) 464, 665
BclI TGATCA 1 cut(s) 414
BfaI CTAG 1 cut(s) 398
BfmI CTRYAG 2 cut(s) 201, 624
BisI GCNGC 5 cut(s) 121, 366, 657, 759, 762
BlsI GCNGC 5 cut(s) 122, 367, 658, 760, 763
BmcAI AGTACT 1 cut(s) 784
Bme1390I CCNGG 2 cut(s) 464, 665
Bme18I GGWCC 1 cut(s) 473
BmgBI CACGTC 1 cut(s) 487
BmgT120I GGNCC 2 cut(s) 210, 473
BmiI GGNNCC 2 cut(s) 309, 474
BmrFI CCNGG 2 cut(s) 464, 665
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 3 cut(s) 113, 620, 766
BmuI ACTGGG 1 cut(s) 315
BpmI CTGGAG 3 cut(s) 539, 681, 729
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 463
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 469, 728
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 3 cut(s) 321, 698, 780
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 2 cut(s) 464, 665
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 3 cut(s) 61, 104, 611
BseLI CCNNNNNNNGG 3 cut(s) 18, 469, 728
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 2 cut(s) 81, 564
BseNI ACTGG 3 cut(s) 321, 698, 780
BseRI GAGGAG 2 cut(s) 374, 536
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 4 cut(s) 352, 643, 748, 770
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 212
BshNI GGYRCC 1 cut(s) 307
BsiSI CCGG 2 cut(s) 12, 596
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 3 cut(s) 18, 469, 728
BsmFI GGGAC 1 cut(s) 426
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 4 cut(s) 58, 289, 414, 683
BspACI CCGC 2 cut(s) 120, 795
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 2 cut(s) 82, 565
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 309, 474
BspPI GGATC 1 cut(s) 691
BspT107I GGYRCC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 595
BsrI ACTGG 3 cut(s) 321, 698, 780
BssAI RCCGGY 1 cut(s) 595
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 4 cut(s) 58, 289, 414, 683
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 464, 665
Bst4CI ACNGT 2 cut(s) 202, 440
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 656
BstC8I GCNNGC 3 cut(s) 282, 395, 705
BstDEI CTNAG 4 cut(s) 62, 90, 303, 573
BstENI CCTNNNNNAGG 1 cut(s) 726
BstF5I GGATG 3 cut(s) 61, 104, 611
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 2 cut(s) 120, 661
BstKTI GATC 4 cut(s) 61, 292, 417, 686
BstMBI GATC 4 cut(s) 58, 289, 414, 683
BstMWI GCNNNNNNNGC 4 cut(s) 362, 629, 656, 665
BstNI CCWGG 2 cut(s) 464, 665
BstSCI CCNGG 2 cut(s) 462, 663
BstSFI CTRYAG 2 cut(s) 201, 624
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 352, 643, 748, 770
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
BsuRI GGCC 1 cut(s) 212
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 3 cut(s) 61, 104, 611
BtsIMutI CAGTG 3 cut(s) 66, 354, 705
Cac8I GCNNGC 3 cut(s) 282, 395, 705
CaiI CAGNNNCTG 3 cut(s) 656, 704, 724
CciI TCATGA 1 cut(s) 43
CfoI GCGC 2 cut(s) 120, 661
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 2 cut(s) 210, 473
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 308, 783
CviAII CATG 4 cut(s) 44, 260, 326, 679
CviQI GTAC 2 cut(s) 308, 783
DdeI CTNAG 4 cut(s) 62, 90, 303, 573
DpnI GATC 4 cut(s) 60, 291, 416, 685
DpnII GATC 4 cut(s) 58, 289, 414, 683
DraIII CACNNNGTG 1 cut(s) 514
Eco32I GATATC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 726
EcoO109I RGGNCCY 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 462, 663
EcoRV GATATC 1 cut(s) 96
FaeI CATG 4 cut(s) 47, 263, 329, 682
FaqI GGGAC 1 cut(s) 426
FatI CATG 4 cut(s) 43, 259, 325, 678
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 5 cut(s) 121, 366, 657, 759, 762
FokI GGATG 3 cut(s) 68, 91, 598
Fsp4HI GCNGC 5 cut(s) 121, 366, 657, 759, 762
FspBI CTAG 1 cut(s) 398
FspI TGCGCA 1 cut(s) 660
GlaI GCGC 2 cut(s) 119, 660
GluI GCNGC 5 cut(s) 121, 366, 657, 759, 762
GsuI CTGGAG 3 cut(s) 539, 681, 729
HaeIII GGCC 1 cut(s) 212
HapII CCGG 2 cut(s) 12, 596
HgaI GACGC 1 cut(s) 112
HhaI GCGC 2 cut(s) 120, 661
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 4 cut(s) 47, 263, 329, 682
Hin6I GCGC 2 cut(s) 118, 659
HinP1I GCGC 2 cut(s) 118, 659
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 4 cut(s) 40, 344, 554, 579
HpaII CCGG 2 cut(s) 12, 596
HphI GGTGA 2 cut(s) 580, 650
Hpy188I TCNGA 5 cut(s) 21, 100, 427, 688, 726
Hpy188III TCNNGA 2 cut(s) 44, 476
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 2 cut(s) 202, 440
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 2 cut(s) 137, 331
HpyF10VI GCNNNNNNNGC 4 cut(s) 362, 629, 656, 665
HpyF3I CTNAG 4 cut(s) 62, 90, 303, 573
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 4 cut(s) 47, 263, 329, 682
HspAI GCGC 2 cut(s) 118, 659
KpnI GGTACC 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 414
Kzo9I GATC 4 cut(s) 58, 289, 414, 683
LmnI GCTCC 2 cut(s) 115, 190
Lsp1109I GCAGC 4 cut(s) 352, 643, 748, 770
LweI GCATC 3 cut(s) 113, 620, 766
MaeI CTAG 1 cut(s) 398
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 434
MalI GATC 4 cut(s) 60, 291, 416, 685
MboI GATC 4 cut(s) 58, 289, 414, 683
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 5 cut(s) 32, 132, 271, 315, 788
MseI TTAA 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 2 cut(s) 12, 596
MspR9I CCNGG 2 cut(s) 464, 665
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 331
MvaI CCWGG 2 cut(s) 464, 665
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 4 cut(s) 362, 629, 656, 665
NdeII GATC 4 cut(s) 58, 289, 414, 683
NlaIII CATG 4 cut(s) 47, 263, 329, 682
NlaIV GGNNCC 2 cut(s) 309, 474
NmeAIII GCCGAG 1 cut(s) 587
NmuCI GTSAC 1 cut(s) 434
NsbI TGCGCA 1 cut(s) 660
PagI TCATGA 1 cut(s) 43
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 4 cut(s) 40, 344, 554, 579
PkrI GCNGC 5 cut(s) 122, 367, 658, 760, 763
PpuMI RGGWCCY 1 cut(s) 473
PsiI TTATAA 2 cut(s) 192, 548
Psp5II RGGWCCY 1 cut(s) 473
Psp6I CCWGG 2 cut(s) 462, 663
PspGI CCWGG 2 cut(s) 462, 663
PspN4I GGNNCC 2 cut(s) 309, 474
PspPI GGNCC 2 cut(s) 210, 473
PspPPI RGGWCCY 1 cut(s) 473
PstNI CAGNNNCTG 3 cut(s) 656, 704, 724
PsuI RGATCY 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 309, 784
RsaNI GTAC 2 cut(s) 308, 783
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 5 cut(s) 121, 366, 657, 759, 762
Sau3AI GATC 4 cut(s) 58, 289, 414, 683
Sau96I GGNCC 2 cut(s) 210, 473
ScaI AGTACT 1 cut(s) 784
ScrFI CCNGG 2 cut(s) 464, 665
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 3 cut(s) 113, 620, 766
SfcI CTRYAG 2 cut(s) 201, 624
SinI GGWCC 1 cut(s) 473
Sse9I AATT 5 cut(s) 32, 132, 271, 315, 788
SsiI CCGC 2 cut(s) 120, 795
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 2 cut(s) 462, 663
TaaI ACNGT 2 cut(s) 202, 440
TaiI ACGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 672
TasI AATT 5 cut(s) 32, 132, 271, 315, 788
TatI WGTACW 1 cut(s) 782
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 4 cut(s) 40, 344, 554, 579
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 3 cut(s) 73, 354, 705
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 4 cut(s) 365, 656, 758, 761
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 3 cut(s) 32, 60, 546
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 3 cut(s) 73, 354, 705
VpaK11BI GGWCC 1 cut(s) 473
XagI CCTNNNNNAGG 1 cut(s) 726
XspI CTAG 1 cut(s) 398
ZrmI AGTACT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.