Rorug04G0306100

6-phosphogluconolactonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006227
Physical Location & Seq
Forward (+)
48237675 .. 48239558
1884 bp
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UTR
Exon/CDS
Intron
Rorug04G0306100.1

Sequence Viewer

Length: 549 bp
ATGTCTTCTAGTTTGGAGAATCAGCACCAAGCCGGGTCTTCTTCGTCTTCATGGAGAAGGTTCAATGCCAACAACTCTTCTAGCTCCCCTCACTCTCTCCAAGATCATCAAAGTAACACGAAGCATAAACTAGATAGCGACGAAGATATGTTCACCGTTCCGGACGTCGAGGCTACACCGCCTGATGATAATCCCATTAGCACCTCTACAGTCACCAACAATAATGCCACTAGCAAGAACAACCCTGGGGAAATTGGCCTTTCGGGCAAACGCCGCAGAGGGCGAAACCCCGTAGATAAGGAGTATCGAAGACTCAAAAGCAGATTGCTAAGGAACAGAGTGTCTGCTCAACAAGCCCGTGAAAGGAAAAAGGTTTACGTCAATGACCTGGAATTAAGAGCCAAGGAGTTGGAGGATGCCAATTCTAACTTGGAAGAAAAGATCTCGACCCTCGTTAACGAAAACACCATGCTTCGAAAGGTTCTCATGAATACAAGGCCAAAAGTGGACGAAAGCATAGAGCAAAAGCAGGATCGATCGGTTAAGTAG

Protein Analysis

182

Amino Acids

20.68

Weight (kDa)

9.52

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bZIP_1 PF00170 101 - 160 1.6e-09 bZIP transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 160
AciI CCGC 2 cut(s) 179, 274
AclWI GGATC 1 cut(s) 540
AcyI GRCGYC 1 cut(s) 165
AfiI CCNNNNNNNGG 1 cut(s) 363
AgsI TTSAA 1 cut(s) 64
AjnI CCWGG 2 cut(s) 244, 387
AluBI AGCT 1 cut(s) 84
AluI AGCT 1 cut(s) 84
AlwI GGATC 1 cut(s) 540
Aor13HI TCCGGA 1 cut(s) 160
AoxI GGCC 2 cut(s) 256, 497
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 145, 205
AsuII TTCGAA 1 cut(s) 475
BbsI GAAGAC 3 cut(s) 30, 39, 316
BciT130I CCWGG 2 cut(s) 246, 389
BcnI CCSGG 1 cut(s) 34
BfaI CTAG 4 cut(s) 9, 81, 131, 231
BfmI CTRYAG 1 cut(s) 207
BglI GCCNNNNNGGC 1 cut(s) 264
BglII AGATCT 1 cut(s) 441
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
Bme1390I CCNGG 3 cut(s) 34, 246, 389
BmrFI CCNGG 3 cut(s) 34, 246, 389
BmsI GCATC 1 cut(s) 406
BpiI GAAGAC 3 cut(s) 30, 39, 316
Bpu10I CCTNAGC 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 475
BpuMI CCSGG 1 cut(s) 34
Bsa29I ATCGAT 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 189
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 3 cut(s) 244, 245, 402
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 363
Bse8I GATNNNNATC 1 cut(s) 189
BseAI TCCGGA 1 cut(s) 160
BseBI CCWGG 2 cut(s) 246, 389
BseCI ATCGAT 1 cut(s) 535
BseDI CCNNGG 3 cut(s) 244, 245, 402
BseGI GGATG 1 cut(s) 421
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 363
Bsh1285I CGRYCG 1 cut(s) 539
BshFI GGCC 2 cut(s) 258, 499
BshVI ATCGAT 1 cut(s) 535
BsiEI CGRYCG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 33, 161
BslI CCNNNNNNNGG 1 cut(s) 363
BsnI GGCC 2 cut(s) 258, 499
Bsp119I TTCGAA 1 cut(s) 475
Bsp13I TCCGGA 1 cut(s) 160
Bsp143I GATC 4 cut(s) 103, 441, 532, 536
BspACI CCGC 2 cut(s) 179, 274
BspANI GGCC 2 cut(s) 258, 499
BspDI ATCGAT 1 cut(s) 535
BspEI TCCGGA 1 cut(s) 160
BspHI TCATGA 1 cut(s) 486
BspPI GGATC 1 cut(s) 540
BspT104I TTCGAA 1 cut(s) 475
BssECI CCNNGG 3 cut(s) 244, 245, 402
BssMI GATC 4 cut(s) 103, 441, 532, 536
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 2 cut(s) 246, 389
Bst4CI ACNGT 2 cut(s) 157, 211
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 165
BstBI TTCGAA 1 cut(s) 475
BstDEI CTNAG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 421
BstKTI GATC 4 cut(s) 106, 444, 535, 539
BstMBI GATC 4 cut(s) 103, 441, 532, 536
BstMCI CGRYCG 1 cut(s) 539
BstMWI GCNNNNNNNGC 3 cut(s) 264, 273, 353
BstNI CCWGG 2 cut(s) 246, 389
BstSCI CCNGG 3 cut(s) 32, 244, 387
BstSFI CTRYAG 1 cut(s) 207
BstV2I GAAGAC 3 cut(s) 30, 39, 316
BstX2I RGATCY 1 cut(s) 441
BstXI CCANNNNNNTGG 1 cut(s) 409
BstYI RGATCY 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 535
BsuRI GGCC 2 cut(s) 258, 499
BsuTUI ATCGAT 1 cut(s) 535
BtsCI GGATG 1 cut(s) 421
CciI TCATGA 1 cut(s) 486
ClaI ATCGAT 1 cut(s) 535
CviAII CATG 3 cut(s) 51, 469, 487
CviJI RGCY 7 cut(s) 32, 84, 173, 258, 356, 401, 499
CviKI_1 RGCY 7 cut(s) 32, 84, 173, 258, 356, 401, 499
DdeI CTNAG 1 cut(s) 329
DpnI GATC 4 cut(s) 105, 443, 534, 538
DpnII GATC 4 cut(s) 103, 441, 532, 536
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 402
EcoRII CCWGG 2 cut(s) 244, 387
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 3 cut(s) 54, 472, 490
FaiI YATR 6 cut(s) 52, 126, 149, 470, 488, 518
FatI CATG 3 cut(s) 50, 468, 486
Fnu4HI GCNGC 1 cut(s) 274
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 1 cut(s) 274
FspBI CTAG 4 cut(s) 9, 81, 131, 231
GluI GCNGC 1 cut(s) 274
HaeIII GGCC 2 cut(s) 258, 499
HapII CCGG 2 cut(s) 33, 161
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 3 cut(s) 54, 472, 490
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 2 cut(s) 19, 312
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 2 cut(s) 33, 161
HphI GGTGA 2 cut(s) 145, 205
Hpy166II GTNNAC 4 cut(s) 153, 376, 457, 508
Hpy188III TCNNGA 3 cut(s) 161, 445, 487
Hpy8I GTNNAC 4 cut(s) 153, 376, 457, 508
Hpy99I CGWCG 2 cut(s) 143, 170
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 2 cut(s) 157, 211
HpyCH4IV ACGT 2 cut(s) 165, 378
HpyF10VI GCNNNNNNNGC 3 cut(s) 264, 273, 353
HpyF3I CTNAG 1 cut(s) 329
HpySE526I ACGT 2 cut(s) 165, 378
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 3 cut(s) 54, 472, 490
Kpn2I TCCGGA 1 cut(s) 160
KspAI GTTAAC 1 cut(s) 457
Kzo9I GATC 4 cut(s) 103, 441, 532, 536
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 8 cut(s) 46, 174, 195, 231, 258, 374, 401, 515
LweI GCATC 1 cut(s) 406
MaeI CTAG 4 cut(s) 9, 81, 131, 231
MaeII ACGT 2 cut(s) 165, 378
MaeIII GTNAC 2 cut(s) 113, 211
MalI GATC 4 cut(s) 105, 443, 534, 538
MboI GATC 4 cut(s) 103, 441, 532, 536
MboII GAAGA 7 cut(s) 30, 33, 39, 69, 155, 321, 446
MflI RGATCY 1 cut(s) 441
MluCI AATT 3 cut(s) 252, 392, 421
MlyI GAGTC 1 cut(s) 306
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 6 cut(s) 99, 163, 214, 272, 406, 461
MroI TCCGGA 1 cut(s) 160
MseI TTAA 3 cut(s) 395, 456, 543
MspI CCGG 2 cut(s) 33, 161
MspR9I CCNGG 3 cut(s) 34, 246, 389
MvaI CCWGG 2 cut(s) 246, 389
MwoI GCNNNNNNNGC 3 cut(s) 264, 273, 353
NciI CCSGG 1 cut(s) 34
NdeII GATC 4 cut(s) 103, 441, 532, 536
NlaIII CATG 3 cut(s) 54, 472, 490
NmuCI GTSAC 1 cut(s) 211
NspV TTCGAA 1 cut(s) 475
PagI TCATGA 1 cut(s) 486
PasI CCCWGGG 1 cut(s) 245
PfeI GAWTC 1 cut(s) 19
PkrI GCNGC 1 cut(s) 275
Ple19I CGATCG 1 cut(s) 539
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 244, 387
PspGI CCWGG 2 cut(s) 244, 387
PsuI RGATCY 1 cut(s) 441
PvuI CGATCG 1 cut(s) 539
SaqAI TTAA 3 cut(s) 395, 456, 543
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 4 cut(s) 103, 441, 532, 536
SchI GAGTC 1 cut(s) 306
ScrFI CCNGG 3 cut(s) 34, 246, 389
SetI ASST 8 cut(s) 62, 86, 168, 206, 375, 381, 390, 483
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 1 cut(s) 207
SfuI TTCGAA 1 cut(s) 475
Sse9I AATT 3 cut(s) 252, 392, 421
SsiI CCGC 2 cut(s) 179, 274
SspMI CTAG 4 cut(s) 9, 81, 131, 231
StyD4I CCNGG 3 cut(s) 32, 244, 387
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 157, 211
TaiI ACGT 2 cut(s) 168, 381
TaqI TCGA 5 cut(s) 168, 307, 446, 475, 535
TasI AATT 3 cut(s) 252, 392, 421
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 3 cut(s) 395, 456, 543
Tru9I TTAA 3 cut(s) 395, 456, 543
TseFI GTSAC 1 cut(s) 211
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 2 cut(s) 39, 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XspI CTAG 4 cut(s) 9, 81, 131, 231
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.