MD16G1059500.v1.1

6-phosphogluconolactonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006227
Physical Location & Seq
Reverse (-)
4273324 .. 4276426
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1059500.v1.1.491

Sequence Viewer

Length: 954 bp
ATGAAATACTATAAATATATTATATATAAACAAATTCGTATTGATTCCCATCTTCCACCTTCGTCAGTTTCTTGCTTCTCTGTCACCTCTCCCTCCTCCTTCCTTCTTCTTCCTCCCCTCTGTAAGCATCTTGGTGTTGAACTTGTTGGAAGCATGGCACTTTCTGGGGTTCGAAAAGATAGAAAGTTGAGGATTCATGAAAGTTTGGATGAGCTTAGCACTGATTTGGCAGACTACATTGCTGAGCTATCAGAGGCATCCGTGAAGGAGCGCGGCGTCTTTGCAATTGCATTATCTGGTGGTTCTCTCATTGGCTTAATGGGAAAACTGTGTGAAGCTCCTTATAACAAGACTGTAGATTGGGCCAAATGGTATATATTTTGGGCAGACGAGCGTGTTGTGGCGAAAAGTCATGTTGATAGCAACTACAAGCTTGCGAAAGATCGCCTTTTGTCCAAGGTACCTATAATTCCCAGTCATGTGCATTCTATTAATGATTCAGTGTCAGCAGAGGAGGCTGCTGATGACTATGAGTTTGTCATTCGCCAGCTAGTGAAATCTCGGGTGATCAGCGTCTCTGATATAAGTGACTGCCCCAAGTTTGACCTAATCCTCCTGGGAATGGGTCCTGATGGGCATGTCGCCTCACTATTCCCTAACCACTCGGTGCTGGAGGAGAAAGATGAATGGGTGACTTTTATAACTGATTCCCCCAAACCACCACCCGAGAGAATCACATTCACCCTGCCAGTCATCAACTCGGCGTCCAATGTGGCTATAGTCGCAACAGGTGACAGCAAAGCAGAGGCTGCGCACATGGCAGTCGACGACATGGGATCTGACCTCCCTCCAGTGCCGGCTGGAATTGTCCAGCCACTGAAAGGGAAGTTGGTGTGGTTTTTGGACAAGGCAGCTGCCTCAAAACTTGATGATGGTACTCAATTCTCTGCATAG

Protein Analysis

318

Amino Acids

34.71

Weight (kDa)

5.61

Isoelectric Point (pI)

30.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucosamine_iso PF01182 69 - 299 4.8e-73 Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 345, 701
Acc16I TGCGCA 1 cut(s) 813
Acc65I GGTACC 1 cut(s) 460
AccB1I GGYRCC 1 cut(s) 460
AccI GTMKAC 1 cut(s) 825
AccII CGCG 1 cut(s) 273
AciI CCGC 1 cut(s) 273
AclWI GGATC 1 cut(s) 844
AcsI RAATTY 1 cut(s) 33
AcyI GRCGYC 2 cut(s) 276, 764
AdeI CACNNNGTG 1 cut(s) 667
AfaI GTAC 2 cut(s) 462, 937
AfiI CCNNNNNNNGG 2 cut(s) 622, 881
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 615
AjuI GAANNNNNNNTTGG 2 cut(s) 872, 904
AluBI AGCT 6 cut(s) 214, 247, 338, 433, 550, 914
AluI AGCT 6 cut(s) 214, 247, 338, 433, 550, 914
Alw26I GTCTC 1 cut(s) 580
AlwI GGATC 1 cut(s) 844
AlwNI CAGNNNCTG 2 cut(s) 809, 877
Ama87I CYCGRG 2 cut(s) 561, 725
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 4 cut(s) 518, 809, 911, 914
ApoI RAATTY 1 cut(s) 33
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
AseI ATTAAT 1 cut(s) 492
Asp718I GGTACC 1 cut(s) 460
AspLEI GCGC 2 cut(s) 273, 814
AspS9I GGNCC 2 cut(s) 363, 626
AsuHPI GGTGA 5 cut(s) 76, 577, 703, 733, 803
AsuII TTCGAA 1 cut(s) 172
AvaI CYCGRG 2 cut(s) 561, 725
AvaII GGWCC 1 cut(s) 626
BanI GGYRCC 1 cut(s) 460
BbvI GCAGC 4 cut(s) 505, 796, 901, 923
BccI CCATC 3 cut(s) 57, 626, 926
BciT130I CCWGG 1 cut(s) 617
BclI TGATCA 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 580
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 2 cut(s) 354, 777
BisI GCNGC 5 cut(s) 274, 519, 810, 912, 915
BlpI GCTNAGC 2 cut(s) 215, 243
BlsI GCNGC 5 cut(s) 275, 520, 811, 913, 916
Bme1390I CCNGG 1 cut(s) 617
Bme18I GGWCC 1 cut(s) 626
BmeT110I CYCGRG 2 cut(s) 561, 725
BmgT120I GGNCC 2 cut(s) 363, 626
BmiI GGNNCC 2 cut(s) 462, 627
BmrFI CCNGG 1 cut(s) 617
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 2 cut(s) 136, 266
BmuI ACTGGG 1 cut(s) 468
BpmI CTGGAG 2 cut(s) 692, 834
Bpu1102I GCTNAGC 2 cut(s) 215, 243
Bpu14I TTCGAA 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 48
BsaHI GRCGYC 2 cut(s) 276, 764
BsaJI CCNNGG 2 cut(s) 456, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 881
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 3 cut(s) 474, 749, 851
Bse3DI GCAATG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 2 cut(s) 456, 616
BseGI GGATG 2 cut(s) 214, 257
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 622, 881
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 3 cut(s) 474, 749, 851
BseRI GAGGAG 3 cut(s) 85, 527, 689
BseXI GCAGC 4 cut(s) 505, 796, 901, 923
Bsh1236I CGCG 1 cut(s) 273
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 1 cut(s) 460
BsiHKCI CYCGRG 2 cut(s) 561, 725
BsiSI CCGG 1 cut(s) 857
BslI CCNNNNNNNGG 2 cut(s) 622, 881
BsmAI GTCTC 1 cut(s) 580
BsmBI CGTCTC 1 cut(s) 580
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 1 cut(s) 365
BsoBI CYCGRG 2 cut(s) 561, 725
Bsp119I TTCGAA 1 cut(s) 172
Bsp143I GATC 3 cut(s) 442, 567, 836
Bsp1720I GCTNAGC 2 cut(s) 215, 243
BspACI CCGC 1 cut(s) 273
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 235
BspFNI CGCG 1 cut(s) 273
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 462, 627
BspPI GGATC 1 cut(s) 844
BspT104I TTCGAA 1 cut(s) 172
BspT107I GGYRCC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 856
BsrI ACTGG 3 cut(s) 474, 749, 851
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 2 cut(s) 456, 616
BssMI GATC 3 cut(s) 442, 567, 836
BssNI GRCGYC 2 cut(s) 276, 764
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 2 cut(s) 330, 355
BstACI GRCGYC 2 cut(s) 276, 764
BstAPI GCANNNNNTGC 1 cut(s) 809
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 3 cut(s) 435, 548, 858
BstDEI CTNAG 2 cut(s) 215, 243
BstF5I GGATG 2 cut(s) 214, 257
BstFNI CGCG 1 cut(s) 273
BstHHI GCGC 2 cut(s) 273, 814
BstKTI GATC 3 cut(s) 445, 570, 839
BstMAI GTCTC 1 cut(s) 580
BstMBI GATC 3 cut(s) 442, 567, 836
BstMWI GCNNNNNNNGC 4 cut(s) 515, 782, 809, 818
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 1 cut(s) 615
BstSFI CTRYAG 2 cut(s) 354, 777
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 4 cut(s) 505, 796, 901, 923
BstX2I RGATCY 1 cut(s) 836
BstYI RGATCY 1 cut(s) 836
BsuRI GGCC 1 cut(s) 365
BtsCI GGATG 2 cut(s) 214, 257
BtsIMutI CAGTG 4 cut(s) 219, 507, 858, 875
Cac8I GCNNGC 3 cut(s) 435, 548, 858
CaiI CAGNNNCTG 2 cut(s) 809, 877
CciI TCATGA 1 cut(s) 196
CfoI GCGC 2 cut(s) 273, 814
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 2 cut(s) 363, 626
CseI GACGC 3 cut(s) 265, 562, 753
Csp6I GTAC 2 cut(s) 461, 936
CviAII CATG 7 cut(s) 154, 197, 413, 479, 638, 817, 832
CviQI GTAC 2 cut(s) 461, 936
DdeI CTNAG 2 cut(s) 215, 243
DpnI GATC 3 cut(s) 444, 569, 838
DpnII GATC 3 cut(s) 442, 567, 836
DraIII CACNNNGTG 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 456
Eco47I GGWCC 1 cut(s) 626
Eco88I CYCGRG 2 cut(s) 561, 725
EcoO109I RGGNCCY 1 cut(s) 626
EcoRII CCWGG 1 cut(s) 615
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
Esp3I CGTCTC 1 cut(s) 580
FaeI CATG 7 cut(s) 157, 200, 416, 482, 641, 820, 835
FalI AAGNNNNNCTT 2 cut(s) 432, 464
FatI CATG 7 cut(s) 153, 196, 412, 478, 637, 816, 831
FbaI TGATCA 1 cut(s) 567
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 5 cut(s) 274, 519, 810, 912, 915
FokI GGATG 2 cut(s) 221, 244
Fsp4HI GCNGC 5 cut(s) 274, 519, 810, 912, 915
FspBI CTAG 1 cut(s) 551
FspI TGCGCA 1 cut(s) 813
GlaI GCGC 2 cut(s) 272, 813
GluI GCNGC 5 cut(s) 274, 519, 810, 912, 915
GsuI CTGGAG 2 cut(s) 692, 834
HaeIII GGCC 1 cut(s) 365
HapII CCGG 1 cut(s) 857
HgaI GACGC 3 cut(s) 265, 562, 753
HhaI GCGC 2 cut(s) 273, 814
Hin1I GRCGYC 2 cut(s) 276, 764
Hin1II CATG 7 cut(s) 157, 200, 416, 482, 641, 820, 835
Hin6I GCGC 2 cut(s) 271, 812
HinP1I GCGC 2 cut(s) 271, 812
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 5 cut(s) 44, 193, 497, 707, 732
HpaII CCGG 1 cut(s) 857
HphI GGTGA 5 cut(s) 76, 577, 703, 733, 803
Hpy166II GTNNAC 1 cut(s) 826
Hpy188I TCNGA 3 cut(s) 253, 580, 841
Hpy188III TCNNGA 2 cut(s) 197, 629
Hpy8I GTNNAC 1 cut(s) 826
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 4 cut(s) 69, 109, 113, 259
HpyCH4III ACNGT 2 cut(s) 330, 355
HpyCH4V TGCA 4 cut(s) 284, 290, 484, 950
HpyF10VI GCNNNNNNNGC 4 cut(s) 515, 782, 809, 818
HpyF3I CTNAG 2 cut(s) 215, 243
Hsp92I GRCGYC 2 cut(s) 276, 764
Hsp92II CATG 7 cut(s) 157, 200, 416, 482, 641, 820, 835
HspAI GCGC 2 cut(s) 271, 812
KpnI GGTACC 1 cut(s) 464
KroI GCCGGC 1 cut(s) 856
KroNI GCCGGC 1 cut(s) 858
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 3 cut(s) 442, 567, 836
LmnI GCTCC 2 cut(s) 268, 343
Lsp1109I GCAGC 4 cut(s) 505, 796, 901, 923
LweI GCATC 2 cut(s) 136, 266
MaeI CTAG 1 cut(s) 551
MaeIII GTNAC 4 cut(s) 82, 587, 691, 791
MalI GATC 3 cut(s) 444, 569, 838
MboI GATC 3 cut(s) 442, 567, 836
MboII GAAGA 3 cut(s) 44, 98, 101
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 1 cut(s) 836
MluCI AATT 5 cut(s) 33, 285, 468, 864, 941
MmeI TCCRAC 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 856
MseI TTAA 2 cut(s) 317, 492
MslI CAYNNNNRTG 1 cut(s) 132
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 1 cut(s) 857
MspR9I CCNGG 1 cut(s) 617
MunI CAATTG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 1 cut(s) 273
MwoI GCNNNNNNNGC 4 cut(s) 515, 782, 809, 818
NaeI GCCGGC 1 cut(s) 858
NdeII GATC 3 cut(s) 442, 567, 836
NgoMIV GCCGGC 1 cut(s) 856
NlaIII CATG 7 cut(s) 157, 200, 416, 482, 641, 820, 835
NlaIV GGNNCC 2 cut(s) 462, 627
NmeAIII GCCGAG 1 cut(s) 740
NmuCI GTSAC 4 cut(s) 82, 587, 691, 791
NsbI TGCGCA 1 cut(s) 813
NspI RCATGY 1 cut(s) 641
NspV TTCGAA 1 cut(s) 172
PagI TCATGA 1 cut(s) 196
PctI GAATGC 1 cut(s) 484
PdiI GCCGGC 1 cut(s) 858
PfeI GAWTC 5 cut(s) 44, 193, 497, 707, 732
PkrI GCNGC 5 cut(s) 275, 520, 811, 913, 916
PpuMI RGGWCCY 1 cut(s) 626
PshBI ATTAAT 1 cut(s) 492
PsiI TTATAA 2 cut(s) 345, 701
Psp5II RGGWCCY 1 cut(s) 626
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 2 cut(s) 462, 627
PspPI GGNCC 2 cut(s) 363, 626
PspPPI RGGWCCY 1 cut(s) 626
PstNI CAGNNNCTG 2 cut(s) 809, 877
PsuI RGATCY 1 cut(s) 836
PvuII CAGCTG 1 cut(s) 914
RsaI GTAC 2 cut(s) 462, 937
RsaNI GTAC 2 cut(s) 461, 936
RseI CAYNNNNRTG 1 cut(s) 132
SalI GTCGAC 1 cut(s) 824
SaqAI TTAA 2 cut(s) 317, 492
SatI GCNGC 5 cut(s) 274, 519, 810, 912, 915
Sau3AI GATC 3 cut(s) 442, 567, 836
Sau96I GGNCC 2 cut(s) 363, 626
ScrFI CCNGG 1 cut(s) 617
SfaNI GCATC 2 cut(s) 136, 266
SfcI CTRYAG 2 cut(s) 354, 777
SfuI TTCGAA 1 cut(s) 172
SinI GGWCC 1 cut(s) 626
SmiMI CAYNNNNRTG 1 cut(s) 132
Sse9I AATT 5 cut(s) 33, 285, 468, 864, 941
SsiI CCGC 1 cut(s) 273
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 615
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 2 cut(s) 330, 355
TaqI TCGA 2 cut(s) 172, 825
TasI AATT 5 cut(s) 33, 285, 468, 864, 941
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 5 cut(s) 44, 193, 497, 707, 732
Tru1I TTAA 2 cut(s) 317, 492
Tru9I TTAA 2 cut(s) 317, 492
TscAI CASTG 4 cut(s) 226, 507, 858, 882
TseFI GTSAC 4 cut(s) 82, 587, 691, 791
TseI GCWGC 4 cut(s) 518, 809, 911, 914
Tsp45I GTSAC 4 cut(s) 82, 587, 691, 791
TspDTI ATGAA 4 cut(s) 17, 185, 213, 699
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 4 cut(s) 226, 507, 858, 882
VpaK11BI GGWCC 1 cut(s) 626
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 1 cut(s) 33
XceI RCATGY 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 825
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.