MD13G1063900.v1.1

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009683
Physical Location & Seq
Forward (+)
4410638 .. 4412676
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1063900.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGCATCCTCAGCTTCCTCGCAGTCATCTTCAGCGTCCTCGCAGTCCGCTGACAAGTACGAGGGGTTTGATCTGTTGAAGTGTTCGACCGTCCAAGAGGGGAAGAAAGAAGCGGAGGTCAAGTTACCGTTAGGGTATCGATTTAATCCCACCGAAGATGAGATCGTTGTGTACTTTCTGTTTAACAAGATTATGGACCGCGCAATGCCTACCTACGATCTTATAAAAGAGGTTGATGTGTACGGGTATGATCCCAATCAGCTGCCGAATGGTGACTTCAAACACAATGCTGGTTGCAATGCGGCCTATTACTTTGCCAATAGAGAACCGTTTGATGCTAGTGAGGGGAAGATAATTAAGACGGCTGCAAACGGTGGTTACTGGAAGGTGATCGATGACGAATATGAGGTTTTGTTCAAGGATAGTGAAGTAATCGTAGGGTTTGAAGCCGTCATGAACTTCTACGAGGGGAAGGAGCCAAACGGGACCAAAACTCCCTTTGTCATGAACGAATACAGGCTCAATCCGCATGTGGTACCTGCTCATGTGCTGAATGACAGCATTAGGAATAAGATTGAGAGGTACGTGGTATGCCGAATTATAAACGAGGAGGTTTCGAATCAGCCCGTTATCGACTATAAAAAGGAATCTCTGGAGCTTCTTTTGAAATATGCGTCTGCCGGCACTGTTAAAGACGGAGAACCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

237

Amino Acids

26.62

Weight (kDa)

4.93

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 43 - 174 2.8e-26 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 224, 602
Acc36I ACCTGC 1 cut(s) 547
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AccII CGCG 1 cut(s) 201
AciI CCGC 5 cut(s) 48, 113, 199, 302, 527
AclWI GGATC 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 15
AfaI GTAC 5 cut(s) 59, 173, 242, 537, 584
AgsI TTSAA 5 cut(s) 79, 280, 418, 446, 667
AluBI AGCT 3 cut(s) 14, 262, 658
AluI AGCT 3 cut(s) 14, 262, 658
AlwI GGATC 1 cut(s) 245
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 262, 365
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 2 cut(s) 196, 486
AsuHPI GGTGA 2 cut(s) 284, 400
AsuII TTCGAA 1 cut(s) 617
AvaII GGWCC 2 cut(s) 196, 486
BanI GGYRCC 1 cut(s) 535
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 2 cut(s) 249, 352
BceAI ACGGC 2 cut(s) 378, 434
BfaI CTAG 1 cut(s) 339
BfuAI ACCTGC 1 cut(s) 547
BisI GCNGC 3 cut(s) 263, 303, 366
BlsI GCNGC 3 cut(s) 264, 304, 367
Bme18I GGWCC 2 cut(s) 196, 486
BmgT120I GGNCC 2 cut(s) 196, 486
BmiI GGNNCC 3 cut(s) 477, 487, 537
BmsI GCATC 2 cut(s) 14, 325
BpmI CTGGAG 1 cut(s) 674
Bpu10I CCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 617
Bsa29I ATCGAT 2 cut(s) 139, 393
BsaAI YACGTR 1 cut(s) 586
BsaBI GATNNNNATC 1 cut(s) 255
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 478, 508
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 2 cut(s) 210, 304
Bse8I GATNNNNATC 1 cut(s) 255
BseCI ATCGAT 2 cut(s) 139, 393
BseGI GGATG 1 cut(s) 5
BseJI GATNNNNATC 1 cut(s) 255
BseMI GCAATG 2 cut(s) 210, 304
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 386
BseRI GAGGAG 1 cut(s) 623
BseXI GCAGC 2 cut(s) 249, 352
Bsh1236I CGCG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 535
BshVI ATCGAT 2 cut(s) 139, 393
BsiEI CGRYCG 1 cut(s) 90
BsiSI CCGG 1 cut(s) 681
BslFI GGGAC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 499
BsnI GGCC 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 617
Bsp143I GATC 5 cut(s) 70, 162, 217, 250, 390
BspACI CCGC 5 cut(s) 48, 113, 199, 302, 527
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 1 cut(s) 23
BspDI ATCGAT 2 cut(s) 139, 393
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 2 cut(s) 453, 504
BspLI GGNNCC 3 cut(s) 477, 487, 537
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 1 cut(s) 245
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 535
BsrDI GCAATG 2 cut(s) 210, 304
BsrFI RCCGGY 1 cut(s) 680
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 680
BssMI GATC 5 cut(s) 70, 162, 217, 250, 390
Bst4CI ACNGT 5 cut(s) 91, 129, 330, 374, 688
BstBAI YACGTR 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 682
BstDEI CTNAG 1 cut(s) 10
BstF5I GGATG 1 cut(s) 5
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 5 cut(s) 73, 165, 220, 253, 393
BstMBI GATC 5 cut(s) 70, 162, 217, 250, 390
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 2 cut(s) 11, 526
BstNSI RCATGY 1 cut(s) 533
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 249, 352
Bsu15I ATCGAT 2 cut(s) 139, 393
BsuRI GGCC 1 cut(s) 305
BsuTUI ATCGAT 2 cut(s) 139, 393
BtsCI GGATG 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 684
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 682
CciI TCATGA 2 cut(s) 453, 504
CfoI GCGC 1 cut(s) 203
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 2 cut(s) 196, 486
ClaI ATCGAT 2 cut(s) 139, 393
CseI GACGC 2 cut(s) 24, 663
Csp6I GTAC 5 cut(s) 58, 172, 241, 536, 583
CviAII CATG 4 cut(s) 454, 505, 530, 545
CviJI RGCY 9 cut(s) 14, 262, 305, 365, 449, 478, 520, 625, 658
CviKI_1 RGCY 9 cut(s) 14, 262, 305, 365, 449, 478, 520, 625, 658
CviQI GTAC 5 cut(s) 58, 172, 241, 536, 583
DdeI CTNAG 1 cut(s) 10
DpnI GATC 5 cut(s) 72, 164, 219, 252, 392
DpnII GATC 5 cut(s) 70, 162, 217, 250, 390
Eco47I GGWCC 2 cut(s) 196, 486
Eco57I CTGAAG 1 cut(s) 15
FaeI CATG 4 cut(s) 457, 508, 533, 548
FaqI GGGAC 1 cut(s) 499
FatI CATG 4 cut(s) 453, 504, 529, 544
Fnu4HI GCNGC 3 cut(s) 263, 303, 366
Fsp4HI GCNGC 3 cut(s) 263, 303, 366
FspBI CTAG 1 cut(s) 339
GlaI GCGC 1 cut(s) 202
GluI GCNGC 3 cut(s) 263, 303, 366
GsuI CTGGAG 1 cut(s) 674
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 681
HgaI GACGC 2 cut(s) 24, 663
HhaI GCGC 1 cut(s) 203
Hin1II CATG 4 cut(s) 457, 508, 533, 548
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 2 cut(s) 619, 647
HpaII CCGG 1 cut(s) 681
HphI GGTGA 2 cut(s) 284, 400
Hpy166II GTNNAC 2 cut(s) 172, 241
Hpy188III TCNNGA 3 cut(s) 454, 505, 653
Hpy8I GTNNAC 2 cut(s) 172, 241
HpyAV CCTTC 2 cut(s) 379, 466
HpyCH4III ACNGT 5 cut(s) 91, 129, 330, 374, 688
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 2 cut(s) 297, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 526
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 4 cut(s) 457, 508, 533, 548
HspAI GCGC 1 cut(s) 201
KpnI GGTACC 1 cut(s) 539
KroI GCCGGC 1 cut(s) 680
KroNI GCCGGC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 70, 162, 217, 250, 390
LmnI GCTCC 2 cut(s) 475, 655
LpnPI CCDG 6 cut(s) 276, 367, 502, 552, 638, 694
Lsp1109I GCAGC 2 cut(s) 249, 352
LweI GCATC 2 cut(s) 14, 325
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 3 cut(s) 123, 272, 377
MalI GATC 5 cut(s) 72, 164, 219, 252, 392
MboI GATC 5 cut(s) 70, 162, 217, 250, 390
MboII GAAGA 4 cut(s) 21, 115, 167, 361
MluCI AATT 2 cut(s) 354, 597
MroNI GCCGGC 1 cut(s) 680
MseI TTAA 4 cut(s) 144, 183, 357, 690
MspA1I CMGCKG 2 cut(s) 50, 262
MspI CCGG 1 cut(s) 681
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 2 cut(s) 11, 526
NaeI GCCGGC 1 cut(s) 682
NdeII GATC 5 cut(s) 70, 162, 217, 250, 390
NgoMIV GCCGGC 1 cut(s) 680
NlaIII CATG 4 cut(s) 457, 508, 533, 548
NlaIV GGNNCC 3 cut(s) 477, 487, 537
NmuCI GTSAC 1 cut(s) 272
NspI RCATGY 1 cut(s) 533
NspV TTCGAA 1 cut(s) 617
PagI TCATGA 2 cut(s) 453, 504
PdiI GCCGGC 1 cut(s) 682
PfeI GAWTC 2 cut(s) 619, 647
PkrI GCNGC 3 cut(s) 264, 304, 367
Ppu21I YACGTR 1 cut(s) 586
PsiI TTATAA 2 cut(s) 224, 602
PspN4I GGNNCC 3 cut(s) 477, 487, 537
PspPI GGNCC 2 cut(s) 196, 486
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 5 cut(s) 59, 173, 242, 537, 584
RsaNI GTAC 5 cut(s) 58, 172, 241, 536, 583
SaqAI TTAA 4 cut(s) 144, 183, 357, 690
SatI GCNGC 3 cut(s) 263, 303, 366
Sau3AI GATC 5 cut(s) 70, 162, 217, 250, 390
Sau96I GGNCC 2 cut(s) 196, 486
SfaNI GCATC 2 cut(s) 14, 325
SfuI TTCGAA 1 cut(s) 617
SinI GGWCC 2 cut(s) 196, 486
Sse9I AATT 2 cut(s) 354, 597
SsiI CCGC 5 cut(s) 48, 113, 199, 302, 527
SspMI CTAG 1 cut(s) 339
TaaI ACNGT 5 cut(s) 91, 129, 330, 374, 688
TaiI ACGT 1 cut(s) 588
TaqI TCGA 5 cut(s) 86, 139, 393, 617, 633
TasI AATT 2 cut(s) 354, 597
TatI WGTACW 1 cut(s) 171
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 2 cut(s) 619, 647
Tru1I TTAA 4 cut(s) 144, 183, 357, 690
Tru9I TTAA 4 cut(s) 144, 183, 357, 690
TscAI CASTG 1 cut(s) 691
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 2 cut(s) 262, 365
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 2 cut(s) 470, 521
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 1 cut(s) 691
VpaK11BI GGWCC 2 cut(s) 196, 486
XceI RCATGY 1 cut(s) 533
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.