MD16G1062600.v1.1

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009683
Physical Location & Seq
Forward (+)
4451167 .. 4452234
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1062600.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGCATCCTCAGCTTCCCAATCTGCTGATGAAAACGAGGGGTTTGATCTGTTGAAGTGTTCGACCGTCCAAGAGGGCATGAAAGATGCGGAGATCAGGTTACCGTTAGGGTATCGATTTGATCCCACCGGAGATGAGATTCTTGTGTACTATCTGTTTAACAAGATTATGGACCGCGCGATGCCTACCTACGATCTTATAAAAGAGGTTGATGTGTACGAGTGTGATCCGAATCAGCTGCCGAATGGTGACTTCAGACATACTGCCGATTTCAATGCTGCCTATTACTTCGCCAATAGAGAACCCTTCGACGCTCGTGAAGCCAAGATAATTAAGACGGCTACAGATGGTGGTTACTGGAAGGTGATCGACGACGAAGAGGAGGTTTTGTTCAAGGACAGCGATGTAATCGTAGGGTTTGAAACCGTCATGAAGTTCTACAAGGGGCAGGCGCCGAACGGAACCAAAACCCCCTTTGTCATGAACGAATACAGGCTCAATCCTCGTGTAGTACCTGCTCATGTGCTGAATGAAAGCATTAAGACTAAGATTGAGAGGTACGTGGTATGCCGAATTATAAACAAGGAGGTTTCGAATCAACCAGCAATCGATTACGGACAGGGATTGCTCGAACTACTGCAAAAATCGTCTGCCGGCACCGTTGAAGATGGAGTACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

228

Amino Acids

25.71

Weight (kDa)

4.72

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 35 - 166 2.6e-26 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 200, 578
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 2 cut(s) 451, 656
AccII CGCG 2 cut(s) 177, 179
AciI CCGC 2 cut(s) 89, 175
AclWI GGATC 2 cut(s) 116, 221
AcuI CTGAAG 1 cut(s) 238
AcyI GRCGYC 1 cut(s) 452
AfaI GTAC 5 cut(s) 149, 218, 513, 560, 675
AgsI TTSAA 5 cut(s) 55, 274, 394, 422, 665
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 2 cut(s) 14, 238
AluI AGCT 2 cut(s) 14, 238
AlwI GGATC 2 cut(s) 116, 221
ApeKI GCWGC 2 cut(s) 238, 278
AspLEI GCGC 2 cut(s) 179, 454
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 260, 376
AsuII TTCGAA 1 cut(s) 593
AvaII GGWCC 1 cut(s) 172
BanI GGYRCC 2 cut(s) 451, 656
BarI GAAGNNNNNNTAC 1 cut(s) 657
BauI CACGAG 2 cut(s) 315, 504
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 2 cut(s) 225, 265
BccI CCATC 2 cut(s) 341, 662
BceAI ACGGC 1 cut(s) 354
BcgI CGANNNNNNTGC 4 cut(s) 220, 254, 257, 291
BfmI CTRYAG 1 cut(s) 342
BfoI RGCGCY 1 cut(s) 455
BfuAI ACCTGC 1 cut(s) 523
BisI GCNGC 2 cut(s) 239, 279
BlsI GCNGC 2 cut(s) 240, 280
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 3 cut(s) 453, 463, 658
BmsI GCATC 3 cut(s) 14, 76, 171
Bpu10I CCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 593
Bsa29I ATCGAT 2 cut(s) 115, 609
BsaAI YACGTR 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 452
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 374, 404
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 1 cut(s) 362
Bse8I GATNNNNATC 1 cut(s) 231
BseCI ATCGAT 2 cut(s) 115, 609
BseGI GGATG 1 cut(s) 5
BseJI GATNNNNATC 1 cut(s) 231
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 2 cut(s) 225, 265
Bsh1236I CGCG 2 cut(s) 177, 179
Bsh1285I CGRYCG 1 cut(s) 66
BshNI GGYRCC 2 cut(s) 451, 656
BshVI ATCGAT 2 cut(s) 115, 609
BsiEI CGRYCG 1 cut(s) 66
BsiSI CCGG 2 cut(s) 129, 654
Bsp119I TTCGAA 1 cut(s) 593
Bsp143I GATC 6 cut(s) 46, 93, 121, 193, 226, 366
BspACI CCGC 2 cut(s) 89, 175
BspCNI CTCAG 1 cut(s) 23
BspDI ATCGAT 2 cut(s) 115, 609
BspFNI CGCG 2 cut(s) 177, 179
BspHI TCATGA 2 cut(s) 429, 480
BspLI GGNNCC 3 cut(s) 453, 463, 658
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 2 cut(s) 116, 221
BspT104I TTCGAA 1 cut(s) 593
BspT107I GGYRCC 2 cut(s) 451, 656
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 1 cut(s) 362
BssAI RCCGGY 1 cut(s) 653
BssMI GATC 6 cut(s) 46, 93, 121, 193, 226, 366
BssNI GRCGYC 1 cut(s) 452
BssSI CACGAG 2 cut(s) 315, 504
Bst2BI CACGAG 2 cut(s) 315, 504
Bst4CI ACNGT 4 cut(s) 67, 105, 427, 661
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 562
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 450, 655
BstDEI CTNAG 2 cut(s) 10, 546
BstEII GGTNACC 1 cut(s) 99
BstF5I GGATG 1 cut(s) 5
BstFNI CGCG 2 cut(s) 177, 179
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 179, 454
BstKTI GATC 6 cut(s) 49, 96, 124, 196, 229, 369
BstMBI GATC 6 cut(s) 46, 93, 121, 193, 226, 366
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 2 cut(s) 11, 320
BstPI GGTNACC 1 cut(s) 99
BstSFI CTRYAG 1 cut(s) 342
BstUI CGCG 2 cut(s) 177, 179
BstV1I GCAGC 2 cut(s) 225, 265
Bsu15I ATCGAT 2 cut(s) 115, 609
BsuTUI ATCGAT 2 cut(s) 115, 609
BtgZI GCGATG 2 cut(s) 194, 417
BtsCI GGATG 1 cut(s) 5
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 2 cut(s) 450, 655
CciI TCATGA 2 cut(s) 429, 480
CfoI GCGC 2 cut(s) 179, 454
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 172
ClaI ATCGAT 2 cut(s) 115, 609
CseI GACGC 1 cut(s) 320
Csp6I GTAC 5 cut(s) 148, 217, 512, 559, 674
CviAII CATG 4 cut(s) 79, 430, 481, 521
CviJI RGCY 5 cut(s) 14, 238, 323, 341, 496
CviKI_1 RGCY 5 cut(s) 14, 238, 323, 341, 496
CviQI GTAC 5 cut(s) 148, 217, 512, 559, 674
DdeI CTNAG 2 cut(s) 10, 546
DinI GGCGCC 1 cut(s) 453
DpnI GATC 6 cut(s) 48, 95, 123, 195, 228, 368
DpnII GATC 6 cut(s) 46, 93, 121, 193, 226, 366
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 238
Eco91I GGTNACC 1 cut(s) 99
EcoO65I GGTNACC 1 cut(s) 99
EgeI GGCGCC 1 cut(s) 453
EheI GGCGCC 1 cut(s) 453
FaeI CATG 4 cut(s) 82, 433, 484, 524
FaiI YATR 9 cut(s) 80, 170, 200, 261, 431, 482, 522, 568, 578
FatI CATG 4 cut(s) 78, 429, 480, 520
Fnu4HI GCNGC 2 cut(s) 239, 279
Fsp4HI GCNGC 2 cut(s) 239, 279
GlaI GCGC 2 cut(s) 178, 453
GluI GCNGC 2 cut(s) 239, 279
HaeII RGCGCY 1 cut(s) 455
HapII CCGG 2 cut(s) 129, 654
HgaI GACGC 1 cut(s) 320
HhaI GCGC 2 cut(s) 179, 454
Hin1I GRCGYC 1 cut(s) 452
Hin1II CATG 4 cut(s) 82, 433, 484, 524
Hin6I GCGC 2 cut(s) 177, 452
HinP1I GCGC 2 cut(s) 177, 452
HinfI GANTC 3 cut(s) 139, 232, 595
HpaII CCGG 2 cut(s) 129, 654
HphI GGTGA 2 cut(s) 260, 376
Hpy166II GTNNAC 2 cut(s) 148, 217
Hpy188I TCNGA 2 cut(s) 231, 257
Hpy188III TCNNGA 3 cut(s) 317, 430, 481
Hpy8I GTNNAC 2 cut(s) 148, 217
Hpy99I CGWCG 3 cut(s) 314, 374, 377
HpyAV CCTTC 2 cut(s) 316, 355
HpyCH4III ACNGT 4 cut(s) 67, 105, 427, 661
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 1 cut(s) 640
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 320
HpyF3I CTNAG 2 cut(s) 10, 546
HpySE526I ACGT 1 cut(s) 561
Hsp92I GRCGYC 1 cut(s) 452
Hsp92II CATG 4 cut(s) 82, 433, 484, 524
HspAI GCGC 2 cut(s) 177, 452
KasI GGCGCC 1 cut(s) 451
KroI GCCGGC 1 cut(s) 653
KroNI GCCGGC 1 cut(s) 655
Kzo9I GATC 6 cut(s) 46, 93, 121, 193, 226, 366
LpnPI CCDG 9 cut(s) 82, 142, 343, 434, 478, 528, 605, 615, 667
Lsp1109I GCAGC 2 cut(s) 225, 265
LweI GCATC 3 cut(s) 14, 76, 171
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 3 cut(s) 99, 248, 353
MalI GATC 6 cut(s) 48, 95, 123, 195, 228, 368
MboI GATC 6 cut(s) 46, 93, 121, 193, 226, 366
MboII GAAGA 2 cut(s) 389, 677
MluCI AATT 2 cut(s) 330, 573
Mly113I GGCGCC 1 cut(s) 452
MnlI CCTC 9 cut(s) 19, 31, 67, 199, 373, 376, 513, 549, 580
MroNI GCCGGC 1 cut(s) 653
MseI TTAA 3 cut(s) 159, 333, 540
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 2 cut(s) 129, 654
MvnI CGCG 2 cut(s) 177, 179
MwoI GCNNNNNNNGC 2 cut(s) 11, 320
NaeI GCCGGC 1 cut(s) 655
NarI GGCGCC 1 cut(s) 452
NdeII GATC 6 cut(s) 46, 93, 121, 193, 226, 366
NgoMIV GCCGGC 1 cut(s) 653
NlaIII CATG 4 cut(s) 82, 433, 484, 524
NlaIV GGNNCC 3 cut(s) 453, 463, 658
NmuCI GTSAC 1 cut(s) 248
NspV TTCGAA 1 cut(s) 593
PagI TCATGA 2 cut(s) 429, 480
PdiI GCCGGC 1 cut(s) 655
PfeI GAWTC 3 cut(s) 139, 232, 595
PkrI GCNGC 2 cut(s) 240, 280
PluTI GGCGCC 1 cut(s) 455
Ppu21I YACGTR 1 cut(s) 562
PsiI TTATAA 2 cut(s) 200, 578
PspEI GGTNACC 1 cut(s) 99
PspN4I GGNNCC 3 cut(s) 453, 463, 658
PspPI GGNCC 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 5 cut(s) 149, 218, 513, 560, 675
RsaNI GTAC 5 cut(s) 148, 217, 512, 559, 674
SaqAI TTAA 3 cut(s) 159, 333, 540
SatI GCNGC 2 cut(s) 239, 279
Sau3AI GATC 6 cut(s) 46, 93, 121, 193, 226, 366
Sau96I GGNCC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 14, 76, 171
SfcI CTRYAG 1 cut(s) 342
SfoI GGCGCC 1 cut(s) 453
SfuI TTCGAA 1 cut(s) 593
SinI GGWCC 1 cut(s) 172
Sse9I AATT 2 cut(s) 330, 573
SsiI CCGC 2 cut(s) 89, 175
SspDI GGCGCC 1 cut(s) 451
TaaI ACNGT 4 cut(s) 67, 105, 427, 661
TaiI ACGT 1 cut(s) 564
TaqI TCGA 7 cut(s) 62, 115, 309, 369, 593, 609, 630
TasI AATT 2 cut(s) 330, 573
TatI WGTACW 1 cut(s) 147
TfiI GAWTC 3 cut(s) 139, 232, 595
Tru1I TTAA 3 cut(s) 159, 333, 540
Tru9I TTAA 3 cut(s) 159, 333, 540
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 2 cut(s) 238, 278
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 5 cut(s) 45, 95, 446, 497, 546
TspGWI ACGGA 2 cut(s) 474, 630
VpaK11BI GGWCC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.