RLG00000006350

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009683
Physical Location & Seq
Reverse (-)
6461554 .. 6463572
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006350

Sequence Viewer

Length: 744 bp
ATGAGGGGGACTACGGGAGGACATACACAAGAGTTTCAACCGCCATACGTCTCTTCATCTCAGCCCCGGCTTGCAACATCAAAGGCTCTCCACAAAGTCGGTCACAAAGGACGAAACAAAGGAGACTTCGATCTGTTGAAGTGTTCAAAAGTTGATGTTGGGAAGAAAGAAAAAGAGCTGAAGTTACCTACAGGCTATAGGTTTTCTCCGGACGATGATGAGTTAGTTCTGTTTTACTTGGTTAACAAAATTCTAGGTCGTACACTACCCATAACTGATGTTATCAAAGAGATCGATTTATATGAGAATGATCCGGATCAACTGCCTCTAGATGATTACAGACATGGTACCAAGTGCAACGAAGCCTATTATTTCACCAATGTTGAACAAATTTACAGTAGCGAAGGGAAGGTCACCAAGAGAACCACAAAGGGTGGTTACTGGAAGGTAACTAGTGAGGGAAAACAGGTTATATATGGAGACGACAAAGTAGTTGTTGGGTTTAAAACCTCTATGTTATTCAATAAAGGACATGTTCCAGCACCAAAAGGGAGCATAAGTTCCTTTGTCATGCATGAGTACAGAGTTAATCCCAGCATAGTTCCTGTTGATGTGCTTAATGACAGCATAAGGGCAAAGATTGATAGATTTGTGGTTTGCAAAATTGTAAGCAAAGAGGCTCGTCCTGCATCTAAACTTCCGCCAGAACTGCTTAGCCATGGAGAAGATGCAAAATCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

248

Amino Acids

27.69

Weight (kDa)

9.01

Isoelectric Point (pI)

27.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 63 - 196 2.6e-29 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 347
AccIII TCCGGA 2 cut(s) 208, 313
AciI CCGC 2 cut(s) 41, 701
AclWI GGATC 2 cut(s) 305, 324
AcsI RAATTY 2 cut(s) 249, 390
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 3 cut(s) 262, 349, 581
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 5 cut(s) 38, 139, 147, 386, 523
AhlI ACTAGT 1 cut(s) 452
AloI GAACNNNNNNTCC 2 cut(s) 544, 576
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw26I GTCTC 3 cut(s) 55, 117, 474
AlwI GGATC 2 cut(s) 305, 324
Aor13HI TCCGGA 2 cut(s) 208, 313
ApoI RAATTY 2 cut(s) 249, 390
ArsI GACNNNNNNTTYG 2 cut(s) 396, 428
Asp718I GGTACC 1 cut(s) 347
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 2 cut(s) 367, 406
BanI GGYRCC 1 cut(s) 347
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 3 cut(s) 55, 117, 474
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 3 cut(s) 254, 329, 453
BfmI CTRYAG 2 cut(s) 189, 196
BlpI GCTNAGC 1 cut(s) 713
Bme1390I CCNGG 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 1 cut(s) 67
BmsI GCATC 2 cut(s) 698, 718
Bpu1102I GCTNAGC 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 67
Bsa29I ATCGAT 1 cut(s) 294
BsaBI GATNNNNATC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 65, 718
BsaWI WCCGGW 2 cut(s) 208, 313
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bse1I ACTGG 1 cut(s) 446
Bse8I GATNNNNATC 1 cut(s) 315
BseAI TCCGGA 2 cut(s) 208, 313
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 65, 718
BseJI GATNNNNATC 1 cut(s) 315
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 446
BseYI CCCAGC 1 cut(s) 593
BshNI GGYRCC 1 cut(s) 347
BshVI ATCGAT 1 cut(s) 294
BsiSI CCGG 3 cut(s) 67, 209, 314
BslFI GGGAC 1 cut(s) 22
BsmAI GTCTC 3 cut(s) 55, 117, 474
BsmBI CGTCTC 2 cut(s) 55, 474
BsmFI GGGAC 1 cut(s) 22
Bsp13I TCCGGA 2 cut(s) 208, 313
Bsp143I GATC 4 cut(s) 130, 291, 310, 316
Bsp1720I GCTNAGC 1 cut(s) 713
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 2 cut(s) 41, 701
BspCNI CTCAG 1 cut(s) 73
BspDI ATCGAT 1 cut(s) 294
BspEI TCCGGA 2 cut(s) 208, 313
BspLI GGNNCC 1 cut(s) 349
BspPI GGATC 2 cut(s) 305, 324
BspT107I GGYRCC 1 cut(s) 347
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 2 cut(s) 65, 718
BssMI GATC 4 cut(s) 130, 291, 310, 316
BssT1I CCWWGG 1 cut(s) 718
Bst4CI ACNGT 1 cut(s) 398
Bst6I CTCTTC 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 2 cut(s) 60, 713
BstDSI CCRYGG 1 cut(s) 718
BstEII GGTNACC 1 cut(s) 412
BstKTI GATC 4 cut(s) 133, 294, 313, 319
BstMAI GTCTC 3 cut(s) 55, 117, 474
BstMBI GATC 4 cut(s) 130, 291, 310, 316
BstMWI GCNNNNNNNGC 2 cut(s) 686, 709
BstNSI RCATGY 1 cut(s) 536
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 1 cut(s) 65
BstSFI CTRYAG 2 cut(s) 189, 196
Bsu15I ATCGAT 1 cut(s) 294
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 718
Cac8I GCNNGC 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 294
Csp6I GTAC 3 cut(s) 261, 348, 580
CviAII CATG 5 cut(s) 344, 533, 571, 575, 719
CviJI RGCY 8 cut(s) 64, 70, 86, 178, 195, 365, 680, 717
CviKI_1 RGCY 8 cut(s) 64, 70, 86, 178, 195, 365, 680, 717
CviQI GTAC 3 cut(s) 261, 348, 580
DdeI CTNAG 2 cut(s) 60, 713
DpnI GATC 4 cut(s) 132, 293, 312, 318
DpnII GATC 4 cut(s) 130, 291, 310, 316
DraI TTTAAA 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 690
Eco130I CCWWGG 1 cut(s) 718
Eco57I CTGAAG 1 cut(s) 200
Eco91I GGTNACC 1 cut(s) 412
EcoO65I GGTNACC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 718
EcoT22I ATGCAT 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 718
Esp3I CGTCTC 2 cut(s) 55, 474
FaeI CATG 5 cut(s) 347, 536, 574, 578, 722
FaqI GGGAC 1 cut(s) 22
FatI CATG 5 cut(s) 343, 532, 570, 574, 718
FspBI CTAG 3 cut(s) 254, 329, 453
GsaI CCCAGC 1 cut(s) 597
HapII CCGG 3 cut(s) 67, 209, 314
Hin1II CATG 5 cut(s) 347, 536, 574, 578, 722
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HpaI GTTAAC 1 cut(s) 244
HpaII CCGG 3 cut(s) 67, 209, 314
HphI GGTGA 2 cut(s) 367, 406
Hpy166II GTNNAC 2 cut(s) 244, 263
Hpy188III TCNNGA 3 cut(s) 209, 314, 329
Hpy8I GTNNAC 2 cut(s) 244, 263
HpyAV CCTTC 3 cut(s) 398, 403, 439
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 6 cut(s) 74, 357, 574, 660, 689, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 709
HpyF3I CTNAG 2 cut(s) 60, 713
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 5 cut(s) 347, 536, 574, 578, 722
Kpn2I TCCGGA 2 cut(s) 208, 313
KpnI GGTACC 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 244
Kzo9I GATC 4 cut(s) 130, 291, 310, 316
LmnI GCTCC 1 cut(s) 552
LweI GCATC 2 cut(s) 698, 718
MaeI CTAG 3 cut(s) 254, 329, 453
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 5 cut(s) 101, 183, 412, 437, 448
MalI GATC 4 cut(s) 132, 293, 312, 318
MboI GATC 4 cut(s) 130, 291, 310, 316
MboII GAAGA 3 cut(s) 45, 175, 737
MluCI AATT 4 cut(s) 249, 390, 663, 739
MnlI CCTC 5 cut(s) 11, 336, 451, 520, 670
Mph1103I ATGCAT 1 cut(s) 576
MroI TCCGGA 2 cut(s) 208, 313
MseI TTAA 4 cut(s) 243, 504, 588, 618
MspI CCGG 3 cut(s) 67, 209, 314
MspR9I CCNGG 1 cut(s) 67
MwoI GCNNNNNNNGC 2 cut(s) 686, 709
NciI CCSGG 1 cut(s) 67
NcoI CCATGG 1 cut(s) 718
NdeII GATC 4 cut(s) 130, 291, 310, 316
NlaIII CATG 5 cut(s) 347, 536, 574, 578, 722
NlaIV GGNNCC 1 cut(s) 349
NmuCI GTSAC 2 cut(s) 101, 412
NsiI ATGCAT 1 cut(s) 576
NspI RCATGY 1 cut(s) 536
PciI ACATGT 1 cut(s) 532
PscI ACATGT 1 cut(s) 532
PspEI GGTNACC 1 cut(s) 412
PspFI CCCAGC 1 cut(s) 593
PspN4I GGNNCC 1 cut(s) 349
RsaI GTAC 3 cut(s) 262, 349, 581
RsaNI GTAC 3 cut(s) 261, 348, 580
SaqAI TTAA 4 cut(s) 243, 504, 588, 618
Sau3AI GATC 4 cut(s) 130, 291, 310, 316
ScrFI CCNGG 1 cut(s) 67
SetI ASST 9 cut(s) 51, 180, 190, 203, 259, 414, 450, 471, 512
SfaNI GCATC 2 cut(s) 698, 718
SfcI CTRYAG 2 cut(s) 189, 196
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 4 cut(s) 249, 390, 663, 739
SsiI CCGC 2 cut(s) 41, 701
SspMI CTAG 3 cut(s) 254, 329, 453
StyD4I CCNGG 1 cut(s) 65
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 1 cut(s) 398
TaiI ACGT 1 cut(s) 51
TaqI TCGA 2 cut(s) 129, 294
TaqII GACCGA 1 cut(s) 89
TasI AATT 4 cut(s) 249, 390, 663, 739
TatI WGTACW 1 cut(s) 579
Tru1I TTAA 4 cut(s) 243, 504, 588, 618
Tru9I TTAA 4 cut(s) 243, 504, 588, 618
TseFI GTSAC 2 cut(s) 101, 412
Tsp45I GTSAC 2 cut(s) 101, 412
TspDTI ATGAA 1 cut(s) 45
XapI RAATTY 2 cut(s) 249, 390
XbaI TCTAGA 1 cut(s) 328
XceI RCATGY 1 cut(s) 536
XspI CTAG 3 cut(s) 254, 329, 453
Zsp2I ATGCAT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.