MD13G1065900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015378
Physical Location & Seq
Forward (+)
4561300 .. 4562871
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1065900.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGTTTCCTCTTCAGTCTCAGCTCTCTTCCGCTCCCATCCTTCCCTTCATATTCCACACACCAATTTAGATGTCCATAAAAGACCTACCCCAGTAGTAGTTTGTTCTAGTTCTTCTCCGGCGCCGACGTCTCCCCGCCATGTTCTTCTCCAAACCTCTCTTTCTTTGGCTGCTTCAGCTGCCATTCTTCTCGGCTCAAATCCAGCTAGTGCTGGATTACTATCAGGATCGACTGGAATTGAATCAGTTCCAGGTCCTGAGCTGCCTCAAATCGACTTCCTCAAACGCTTCAACGAAGAAAACCAGAAAAAGTACGCAGAAAATGATGCAAGATTCAGATCAACTCCAATACTCAAGGAGCTACTAGAAAAATCCAAGCTCAACAAAGAAAAGAATAGTAAAGAAATTCAAGACAAGTACTGCATACGGGGGGCAGAGTGGGGAGTTGGAGATTGCTCGGCAGAGGGAATGACACCTGATGAGAAAGACAAAGTTCATCGCAATGTTGAAGGAGGAGGCTGGCGTCAAGGATTGATATCCACTGGCTTATGCTTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.12

Weight (kDa)

8.35

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03420
fragaria_vesca FvH4_4g30480
malus_domestica MD13G1065900.v1.1 MD16G1064700.v1.1
prunus_persica Prupe.1G282200_v2.0.a1
pyrus_communis pycom13g05810 pycom16g05650
rosa_chinensis RchiOBHm_Chr4g0438851
rosa_laevigata RLG00000006325
rosa_rugosa Rorug04G0313400
rosa_samantha Rh4AG364500 Rh4BG375600 Rh4CG389700 Rh4DG370300
rosa_wichuraiana Rw0G007260 Rw0G016810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 121
AccBSI CCGCTC 1 cut(s) 33
AciI CCGC 2 cut(s) 31, 136
AclWI GGATC 1 cut(s) 235
AcsI RAATTY 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 159
AcyI GRCGYC 3 cut(s) 122, 128, 523
AfaI GTAC 2 cut(s) 314, 419
AgsI TTSAA 4 cut(s) 242, 292, 410, 509
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 6 cut(s) 23, 179, 206, 262, 361, 379
AluI AGCT 6 cut(s) 23, 179, 206, 262, 361, 379
Alw26I GTCTC 2 cut(s) 22, 135
AlwI GGATC 1 cut(s) 235
AlwNI CAGNNNCTG 1 cut(s) 257
ApeKI GCWGC 3 cut(s) 170, 179, 262
ApoI RAATTY 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 246
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 254
AvaII GGWCC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 121
BbvI GCAGC 3 cut(s) 157, 166, 249
BccI CCATC 1 cut(s) 45
BciT130I CCWGG 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 22, 135
BfaI CTAG 3 cut(s) 108, 207, 365
BfoI RGCGCY 1 cut(s) 125
BisI GCNGC 3 cut(s) 171, 180, 263
BlsI GCNGC 3 cut(s) 172, 181, 264
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 252
BmrI ACTGGG 1 cut(s) 86
BmsI GCATC 1 cut(s) 316
BmuI ACTGGG 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 338
BsaBI GATNNNNATC 3 cut(s) 220, 337, 535
BsaHI GRCGYC 3 cut(s) 122, 128, 523
Bse1I ACTGG 3 cut(s) 92, 238, 547
Bse3DI GCAATG 1 cut(s) 508
Bse8I GATNNNNATC 3 cut(s) 220, 337, 535
BseBI CCWGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 37
BseJI GATNNNNATC 3 cut(s) 220, 337, 535
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 2 cut(s) 33, 249
BseNI ACTGG 3 cut(s) 92, 238, 547
BseRI GAGGAG 1 cut(s) 528
BseXI GCAGC 3 cut(s) 157, 166, 249
BshNI GGYRCC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 119
BsmAI GTCTC 2 cut(s) 22, 135
BsmBI CGTCTC 1 cut(s) 135
Bsp143I GATC 2 cut(s) 227, 338
BspACI CCGC 2 cut(s) 31, 136
BspCNI CTCAG 2 cut(s) 32, 250
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 1 cut(s) 235
BspT107I GGYRCC 1 cut(s) 121
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 3 cut(s) 92, 238, 547
BssMI GATC 2 cut(s) 227, 338
BssNI GRCGYC 3 cut(s) 122, 128, 523
Bst2UI CCWGG 1 cut(s) 252
Bst6I CTCTTC 2 cut(s) 16, 32
BstACI GRCGYC 3 cut(s) 122, 128, 523
BstC8I GCNNGC 1 cut(s) 521
BstDEI CTNAG 2 cut(s) 19, 258
BstF5I GGATG 1 cut(s) 37
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 2 cut(s) 230, 341
BstMAI GTCTC 2 cut(s) 22, 135
BstMBI GATC 2 cut(s) 227, 338
BstMWI GCNNNNNNNGC 2 cut(s) 176, 179
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 157, 166, 249
BtgZI GCGATG 1 cut(s) 482
BtsCI GGATG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 540
Cac8I GCNNGC 1 cut(s) 521
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 1 cut(s) 512
Csp6I GTAC 2 cut(s) 313, 418
CviAII CATG 1 cut(s) 140
CviQI GTAC 2 cut(s) 313, 418
DdeI CTNAG 2 cut(s) 19, 258
DinI GGCGCC 1 cut(s) 123
DpnI GATC 2 cut(s) 229, 340
DpnII GATC 2 cut(s) 227, 338
Eam1104I CTCTTC 2 cut(s) 16, 32
EarI CTCTTC 2 cut(s) 16, 32
Eco32I GATATC 1 cut(s) 537
Eco47I GGWCC 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 250
EcoRV GATATC 1 cut(s) 537
EgeI GGCGCC 1 cut(s) 123
EheI GGCGCC 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 1 cut(s) 143
FaiI YATR 5 cut(s) 51, 78, 141, 425, 550
FatI CATG 1 cut(s) 139
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 3 cut(s) 171, 180, 263
FokI GGATG 1 cut(s) 24
Fsp4HI GCNGC 3 cut(s) 171, 180, 263
FspBI CTAG 3 cut(s) 108, 207, 365
GlaI GCGC 1 cut(s) 123
GluI GCNGC 3 cut(s) 171, 180, 263
HaeII RGCGCY 1 cut(s) 125
HapII CCGG 1 cut(s) 119
HgaI GACGC 1 cut(s) 512
HhaI GCGC 1 cut(s) 124
Hin1I GRCGYC 3 cut(s) 122, 128, 523
Hin1II CATG 1 cut(s) 143
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HinfI GANTC 2 cut(s) 242, 333
HpaII CCGG 1 cut(s) 119
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 3 cut(s) 225, 257, 410
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 3 cut(s) 51, 56, 503
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 2 cut(s) 329, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 179
HpyF3I CTNAG 2 cut(s) 19, 258
HpySE526I ACGT 1 cut(s) 128
Hsp92I GRCGYC 3 cut(s) 122, 128, 523
Hsp92II CATG 1 cut(s) 143
HspAI GCGC 1 cut(s) 122
KasI GGCGCC 1 cut(s) 121
Kzo9I GATC 2 cut(s) 227, 338
LmnI GCTCC 2 cut(s) 38, 358
Lsp1109I GCAGC 3 cut(s) 157, 166, 249
LweI GCATC 1 cut(s) 316
MaeI CTAG 3 cut(s) 108, 207, 365
MaeII ACGT 1 cut(s) 128
MalI GATC 2 cut(s) 229, 340
MbiI CCGCTC 1 cut(s) 33
MboI GATC 2 cut(s) 227, 338
MboII GAAGA 6 cut(s) 3, 19, 105, 137, 179, 308
MluCI AATT 3 cut(s) 64, 237, 405
Mly113I GGCGCC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 7 cut(s) 19, 166, 276, 290, 457, 506, 509
MroXI GAANNNNTTC 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 176, 179
NarI GGCGCC 1 cut(s) 122
NdeII GATC 2 cut(s) 227, 338
NlaIII CATG 1 cut(s) 143
NlaIV GGNNCC 1 cut(s) 123
NmeAIII GCCGAG 2 cut(s) 171, 437
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 2 cut(s) 242, 333
PkrI GCNGC 3 cut(s) 172, 181, 264
PluTI GGCGCC 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 254
PspPPI RGGWCCY 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 179
RsaI GTAC 2 cut(s) 314, 419
RsaNI GTAC 2 cut(s) 313, 418
RseI CAYNNNNRTG 1 cut(s) 501
SatI GCNGC 3 cut(s) 171, 180, 263
Sau3AI GATC 2 cut(s) 227, 338
Sau96I GGNCC 1 cut(s) 254
ScaI AGTACT 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 252
SfaNI GCATC 1 cut(s) 316
SfoI GGCGCC 1 cut(s) 123
SinI GGWCC 1 cut(s) 254
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 3 cut(s) 64, 237, 405
SsiI CCGC 2 cut(s) 31, 136
SspDI GGCGCC 1 cut(s) 121
SspMI CTAG 3 cut(s) 108, 207, 365
StyD4I CCNGG 1 cut(s) 250
TaiI ACGT 1 cut(s) 131
TaqI TCGA 2 cut(s) 230, 273
TasI AATT 3 cut(s) 64, 237, 405
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 2 cut(s) 242, 333
TscAI CASTG 1 cut(s) 547
TseI GCWGC 3 cut(s) 170, 179, 262
TspDTI ATGAA 2 cut(s) 38, 485
TspRI CASTG 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 3 cut(s) 108, 207, 365
ZraI GACGTC 1 cut(s) 129
ZrmI AGTACT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.