pycom16g05650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015378
Physical Location & Seq
Forward (+)
3625262 .. 3626705
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g05650.3

Sequence Viewer

Length: 576 bp
ATGGCTTCCTCTTCATCTTCAGCTCTCTCCCACTCTTCCTTTAATATTTCAAGCACCAATTTAAATGTCTGGGAAAAACCTGGTAACCTGCATAGCAGAGTCCAAGTAAAGAGCTGTAAAAGACCTACCACCGTCGTAGTTTGTTCTAGTTCTTCTTCTCCGGCTCCGACGTCTCGCCGCCGCGTTCTTCTCCAAGGCTCCGTTTCTTTGGCTGCTTCAGCTGCCATTCTCCTCTGCTCAAATCCAGCTGAAGCTGGATTTCTATCAGGATCGACCGGAATAGAATCAGTTCCGGGTCCTGAGTTGCCTCAAATCGACTTCCTCAAACGCTTCAATGAAGAAAACCAGAAAAAGTATGCAGAAAATGATGCAAGATTCAGATCAACTCAAATACTCAAGGAGCTACTAGAAAAATCCAAGCTCAACAAAGAAAAGAATAGTAAGGAAATTCAAGACAAGTACTGCATACGCGGAGCGGAGTGGGGAGTTGGAGATTGCTCAGCGGAGGGAATGACACCTGACGAGAAAGACAAGTTCATCGCAATGTTGAAGGAGAAGGCTGGTGTCAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

20.76

Weight (kDa)

8.95

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03420
fragaria_vesca FvH4_4g30480
malus_domestica MD13G1065900.v1.1 MD16G1064700.v1.1
prunus_persica Prupe.1G282200_v2.0.a1
pyrus_communis pycom13g05810 pycom16g05650
rosa_chinensis RchiOBHm_Chr4g0438851
rosa_laevigata RLG00000006325
rosa_rugosa Rorug04G0313400
rosa_samantha Rh4AG364500 Rh4BG375600 Rh4CG389700 Rh4DG370300
rosa_wichuraiana Rw0G007260 Rw0G016810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 96
AccBSI CCGCTC 1 cut(s) 476
AccII CGCG 2 cut(s) 183, 471
AciI CCGC 5 cut(s) 178, 181, 471, 476, 503
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 2 cut(s) 201, 270
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 1 cut(s) 461
AgsI TTSAA 4 cut(s) 51, 334, 452, 550
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 7 cut(s) 23, 114, 221, 248, 254, 403, 421
AluI AGCT 7 cut(s) 23, 114, 221, 248, 254, 403, 421
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 212, 221
ApoI RAATTY 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 294
AvaII GGWCC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 199, 208
BciT130I CCWGG 1 cut(s) 81
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 2 cut(s) 147, 407
BfuAI ACCTGC 1 cut(s) 96
BisI GCNGC 4 cut(s) 178, 181, 213, 222
BlpI GCTNAGC 1 cut(s) 499
BlsI GCNGC 4 cut(s) 179, 182, 214, 223
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 2 cut(s) 81, 294
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 3 cut(s) 165, 199, 297
BmrFI CCNGG 2 cut(s) 81, 294
BmsI GCATC 1 cut(s) 358
Bpu1102I GCTNAGC 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 294
BsaBI GATNNNNATC 2 cut(s) 262, 379
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 193
BsaWI WCCGGW 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 549
Bse8I GATNNNNATC 2 cut(s) 262, 379
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 193
BseJI GATNNNNATC 2 cut(s) 262, 379
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 2 cut(s) 291, 513
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 199, 208
Bsh1236I CGCG 2 cut(s) 183, 471
Bsh1285I CGRYCG 1 cut(s) 276
BsiEI CGRYCG 1 cut(s) 276
BsiSI CCGG 3 cut(s) 161, 276, 293
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
Bsp143I GATC 2 cut(s) 269, 380
Bsp1720I GCTNAGC 1 cut(s) 499
BspACI CCGC 5 cut(s) 178, 181, 471, 476, 503
BspCNI CTCAG 2 cut(s) 292, 512
BspFNI CGCG 2 cut(s) 183, 471
BspLI GGNNCC 3 cut(s) 165, 199, 297
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 476
BsrDI GCAATG 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 2 cut(s) 269, 380
BssNI GRCGYC 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 2 cut(s) 16, 40
BstACI GRCGYC 1 cut(s) 170
BstDEI CTNAG 2 cut(s) 300, 499
BstEII GGTNACC 1 cut(s) 83
BstFNI CGCG 2 cut(s) 183, 471
BstKTI GATC 2 cut(s) 272, 383
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 269, 380
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 2 cut(s) 218, 221
BstNI CCWGG 1 cut(s) 81
BstPI GGTNACC 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 79, 292
BstUI CGCG 2 cut(s) 183, 471
BstV1I GCAGC 2 cut(s) 199, 208
BtgZI GCGATG 1 cut(s) 523
BveI ACCTGC 1 cut(s) 96
Cfr13I GGNCC 1 cut(s) 296
CsiI ACCWGGT 1 cut(s) 79
Csp6I GTAC 1 cut(s) 460
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 2 cut(s) 300, 499
DpnI GATC 2 cut(s) 271, 382
DpnII GATC 2 cut(s) 269, 380
DraI TTTAAA 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 16, 40
EarI CTCTTC 2 cut(s) 16, 40
Eco130I CCWWGG 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 2 cut(s) 201, 270
Eco91I GGTNACC 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 193
Esp3I CGTCTC 1 cut(s) 177
FaiI YATR 3 cut(s) 93, 357, 467
Fnu4HI GCNGC 4 cut(s) 178, 181, 213, 222
Fsp4HI GCNGC 4 cut(s) 178, 181, 213, 222
FspBI CTAG 2 cut(s) 147, 407
GluI GCNGC 4 cut(s) 178, 181, 213, 222
HapII CCGG 3 cut(s) 161, 276, 293
Hin1I GRCGYC 1 cut(s) 170
HinfI GANTC 3 cut(s) 99, 284, 375
HpaII CCGG 3 cut(s) 161, 276, 293
Hpy188I TCNGA 2 cut(s) 168, 380
Hpy188III TCNNGA 3 cut(s) 267, 299, 452
Hpy99I CGWCG 2 cut(s) 137, 172
HpyAV CCTTC 2 cut(s) 544, 550
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 4 cut(s) 91, 359, 371, 465
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 221
HpyF3I CTNAG 2 cut(s) 300, 499
HpySE526I ACGT 1 cut(s) 170
Hsp92I GRCGYC 1 cut(s) 170
Kzo9I GATC 2 cut(s) 269, 380
LmnI GCTCC 4 cut(s) 169, 203, 400, 473
Lsp1109I GCAGC 2 cut(s) 199, 208
LweI GCATC 1 cut(s) 358
MabI ACCWGGT 1 cut(s) 79
MaeI CTAG 2 cut(s) 147, 407
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 83
MalI GATC 2 cut(s) 271, 382
MbiI CCGCTC 1 cut(s) 476
MboI GATC 2 cut(s) 269, 380
MboII GAAGA 7 cut(s) 3, 9, 27, 144, 147, 179, 350
MluCI AATT 2 cut(s) 58, 447
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 2 cut(s) 191, 469
MnlI CCTC 5 cut(s) 19, 242, 318, 332, 499
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 2 cut(s) 42, 62
MslI CAYNNNNRTG 1 cut(s) 542
MspA1I CMGCKG 3 cut(s) 221, 248, 503
MspI CCGG 3 cut(s) 161, 276, 293
MspR9I CCNGG 2 cut(s) 81, 294
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 2 cut(s) 183, 471
MwoI GCNNNNNNNGC 2 cut(s) 218, 221
NciI CCSGG 1 cut(s) 294
NdeII GATC 2 cut(s) 269, 380
NlaIV GGNNCC 3 cut(s) 165, 199, 297
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 284, 375
PkrI GCNGC 4 cut(s) 179, 182, 214, 223
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 79
PspEI GGTNACC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 3 cut(s) 165, 199, 297
PspPI GGNCC 1 cut(s) 296
PspPPI RGGWCCY 1 cut(s) 296
PvuII CAGCTG 2 cut(s) 221, 248
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 2 cut(s) 42, 62
SatI GCNGC 4 cut(s) 178, 181, 213, 222
Sau3AI GATC 2 cut(s) 269, 380
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 2 cut(s) 81, 294
SexAI ACCWGGT 1 cut(s) 79
SfaNI GCATC 1 cut(s) 358
SinI GGWCC 1 cut(s) 296
SmiI ATTTAAAT 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 2 cut(s) 58, 447
SsiI CCGC 5 cut(s) 178, 181, 471, 476, 503
SspI AATATT 1 cut(s) 46
SspMI CTAG 2 cut(s) 147, 407
StyD4I CCNGG 2 cut(s) 79, 292
StyI CCWWGG 1 cut(s) 193
SwaI ATTTAAAT 1 cut(s) 63
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 272, 315
TasI AATT 2 cut(s) 58, 447
TatI WGTACW 1 cut(s) 459
TauI GCSGC 2 cut(s) 180, 183
TfiI GAWTC 2 cut(s) 284, 375
Tru1I TTAA 2 cut(s) 42, 62
Tru9I TTAA 2 cut(s) 42, 62
TseI GCWGC 2 cut(s) 212, 221
TspDTI ATGAA 2 cut(s) 351, 526
TspGWI ACGGA 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 2 cut(s) 147, 407
ZraI GACGTC 1 cut(s) 171
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.