MD16G1064700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015378
Physical Location & Seq
Forward (+)
4552992 .. 4554670
1679 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1064700.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGTTTGCTCTTCACCTTCAGCTCTCTCCCACTCTTCCTTTACTATTTCAAGCACCAATTTAAATGTCTGGGAAAAACCTGGTAACCTGCATAGCAGAGTCCAAGTAAAGAGCTGTAAAAGACCTACCACAGTCGTAGTTTGTTCTGGTTCTTCTTCTCCGGCTCCGACGTCTCGCCGCCGCGTTCTTCTCCAAAGCTCCGTTTCTTTGGCTGCTTCAGCTGCCATTCTCCTCTGCTCAAATCCAGCTGAAGCAGGATTTCTATCAGGATCGACCGGAATAGAATCAGTTCCGGGTCCTGAGTTGCCTCAAATCGACTTCCTCAAACGCTTCAATGAAAATCAGAAGAAGTATGCAGAAAATGATGCAAGATTCAGATCAACTCAAATACTCAAGGAGCTACTAGAAAAGTCCAAGCTCAACAAAGAAAAGAATAGTAAGGAAATTCAAGACAAGTACTGCATACGCGGGGCGGAGTGGGGAGTAGGAGATTGCTCAGCGGAGGGAATGACACCTAACGAGAAAGACAAGTTCATCGCAATGTTGAAGGAGAAGGCTGGCGTCAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.67

Weight (kDa)

9.15

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015378)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03420
fragaria_vesca FvH4_4g30480
malus_domestica MD13G1065900.v1.1 MD16G1064700.v1.1
prunus_persica Prupe.1G282200_v2.0.a1
pyrus_communis pycom13g05810 pycom16g05650
rosa_chinensis RchiOBHm_Chr4g0438851
rosa_laevigata RLG00000006325
rosa_rugosa Rorug04G0313400
rosa_samantha Rh4AG364500 Rh4BG375600 Rh4CG389700 Rh4DG370300
rosa_wichuraiana Rw0G007260 Rw0G016810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 96
AccII CGCG 2 cut(s) 183, 468
AciI CCGC 5 cut(s) 178, 181, 468, 473, 500
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 2 cut(s) 201, 270
AcyI GRCGYC 2 cut(s) 170, 561
AfaI GTAC 1 cut(s) 458
AgsI TTSAA 4 cut(s) 51, 334, 449, 547
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 7 cut(s) 23, 114, 198, 221, 248, 400, 418
AluI AGCT 7 cut(s) 23, 114, 198, 221, 248, 400, 418
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 212, 221
ApoI RAATTY 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 6
AvaII GGWCC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 199, 208
BciT130I CCWGG 1 cut(s) 81
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 96
BisI GCNGC 4 cut(s) 178, 181, 213, 222
BlpI GCTNAGC 1 cut(s) 496
BlsI GCNGC 4 cut(s) 179, 182, 214, 223
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 81, 294
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 165, 297
BmrFI CCNGG 2 cut(s) 81, 294
BmsI GCATC 1 cut(s) 355
Bpu1102I GCTNAGC 1 cut(s) 496
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 294
BsaBI GATNNNNATC 2 cut(s) 262, 376
BsaHI GRCGYC 2 cut(s) 170, 561
BsaWI WCCGGW 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 2 cut(s) 262, 376
BseBI CCWGG 1 cut(s) 81
BseJI GATNNNNATC 2 cut(s) 262, 376
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 2 cut(s) 291, 510
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 199, 208
Bsh1236I CGCG 2 cut(s) 183, 468
Bsh1285I CGRYCG 1 cut(s) 276
BsiEI CGRYCG 1 cut(s) 276
BsiSI CCGG 3 cut(s) 161, 276, 293
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
Bsp143I GATC 2 cut(s) 269, 377
Bsp1720I GCTNAGC 1 cut(s) 496
BspACI CCGC 5 cut(s) 178, 181, 468, 473, 500
BspCNI CTCAG 2 cut(s) 292, 509
BspFNI CGCG 2 cut(s) 183, 468
BspLI GGNNCC 2 cut(s) 165, 297
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 1 cut(s) 277
BspQI GCTCTTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 546
BssMI GATC 2 cut(s) 269, 377
BssNI GRCGYC 2 cut(s) 170, 561
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 2 cut(s) 16, 40
BstACI GRCGYC 2 cut(s) 170, 561
BstC8I GCNNGC 1 cut(s) 559
BstDEI CTNAG 2 cut(s) 300, 496
BstEII GGTNACC 1 cut(s) 83
BstFNI CGCG 2 cut(s) 183, 468
BstKTI GATC 2 cut(s) 272, 380
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 269, 377
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 2 cut(s) 218, 221
BstNI CCWGG 1 cut(s) 81
BstPI GGTNACC 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 79, 292
BstUI CGCG 2 cut(s) 183, 468
BstV1I GCAGC 2 cut(s) 199, 208
BtgZI GCGATG 1 cut(s) 520
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 550
CsiI ACCWGGT 1 cut(s) 79
Csp6I GTAC 1 cut(s) 457
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 2 cut(s) 300, 496
DpnI GATC 2 cut(s) 271, 379
DpnII GATC 2 cut(s) 269, 377
DraI TTTAAA 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 16, 40
EarI CTCTTC 2 cut(s) 16, 40
EciI GGCGGA 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 2 cut(s) 201, 270
Eco91I GGTNACC 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 79
Esp3I CGTCTC 1 cut(s) 177
FaiI YATR 3 cut(s) 93, 354, 464
FauI CCCGC 1 cut(s) 461
Fnu4HI GCNGC 4 cut(s) 178, 181, 213, 222
Fsp4HI GCNGC 4 cut(s) 178, 181, 213, 222
FspBI CTAG 1 cut(s) 404
GluI GCNGC 4 cut(s) 178, 181, 213, 222
HapII CCGG 3 cut(s) 161, 276, 293
HgaI GACGC 1 cut(s) 550
Hin1I GRCGYC 2 cut(s) 170, 561
HinfI GANTC 3 cut(s) 99, 284, 372
HpaII CCGG 3 cut(s) 161, 276, 293
HphI GGTGA 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 168, 345, 377
Hpy188III TCNNGA 3 cut(s) 267, 299, 449
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 3 cut(s) 27, 541, 547
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 4 cut(s) 91, 356, 368, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 221
HpyF3I CTNAG 2 cut(s) 300, 496
HpySE526I ACGT 1 cut(s) 170
Hsp92I GRCGYC 2 cut(s) 170, 561
Kzo9I GATC 2 cut(s) 269, 377
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 169, 203, 397
Lsp1109I GCAGC 2 cut(s) 199, 208
LweI GCATC 1 cut(s) 355
MabI ACCWGGT 1 cut(s) 79
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 83
MalI GATC 2 cut(s) 271, 379
MboI GATC 2 cut(s) 269, 377
MboII GAAGA 6 cut(s) 3, 27, 144, 147, 179, 358
MluCI AATT 2 cut(s) 58, 444
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 4 cut(s) 242, 318, 332, 496
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 539
MspA1I CMGCKG 3 cut(s) 221, 248, 500
MspI CCGG 3 cut(s) 161, 276, 293
MspR9I CCNGG 2 cut(s) 81, 294
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 2 cut(s) 183, 468
MwoI GCNNNNNNNGC 2 cut(s) 218, 221
NciI CCSGG 1 cut(s) 294
NdeII GATC 2 cut(s) 269, 377
NlaIV GGNNCC 2 cut(s) 165, 297
PciSI GCTCTTC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 284, 372
PkrI GCNGC 4 cut(s) 179, 182, 214, 223
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 79
PspEI GGTNACC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 165, 297
PspPI GGNCC 1 cut(s) 296
PspPPI RGGWCCY 1 cut(s) 296
PvuII CAGCTG 2 cut(s) 221, 248
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 539
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 4 cut(s) 178, 181, 213, 222
Sau3AI GATC 2 cut(s) 269, 377
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 458
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 2 cut(s) 81, 294
SexAI ACCWGGT 1 cut(s) 79
SfaNI GCATC 1 cut(s) 355
SinI GGWCC 1 cut(s) 296
SmiI ATTTAAAT 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 2 cut(s) 58, 444
SsiI CCGC 5 cut(s) 178, 181, 468, 473, 500
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 79, 292
SwaI ATTTAAAT 1 cut(s) 63
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 272, 315
TasI AATT 2 cut(s) 58, 444
TatI WGTACW 1 cut(s) 456
TauI GCSGC 2 cut(s) 180, 183
TfiI GAWTC 2 cut(s) 284, 372
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseI GCWGC 2 cut(s) 212, 221
TspDTI ATGAA 2 cut(s) 351, 523
TspGWI ACGGA 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 404
ZraI GACGTC 1 cut(s) 171
ZrmI AGTACT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.