MD13G1067400.v1.1

Amino acid kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008655
Physical Location & Seq
Reverse (-)
4627434 .. 4631063
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1067400.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGAGGAGACCAACACAAATGATCTCCAAAGCGCTTCTGTCAGCCTCACGAAACCGATTCGCTGCATCGTCAAACTCGGTCTTCTAGGAGGTGCAGCAGTTACTTGCAAGAACGAATTAGAGACAATAAATGAAGCAAATCTCAAGATGGTTTCGTCGCAGCTGAGGCAATCAATGGCTACGGGGTCGCCTTCTGGGGAGGTGCTTGGGATGGATTGGAGCAAGCAACCTGGAGAATCAGAAATTTCATCTGCCATACTTGATTTTGAACATCGGCCAGTTGCGGACCCCTGCCCCTTTATAGTTGTTCATGGAGCAGGTTCTTTTGGGCATTTTCAAGCTAGTAAATCGGGCGTTCATAAAGGTGGGTTGTACCAACCCCTTGTCAAGGCTGGTTTTGTTGCCACACGCATCTCCGTTACAAAGCTCAATCTAGAAATTGTTAGAGCGTTAGCCAGAGAGGGTATCCCTTCCATTGGAATGTCTCCATTTTCATGTGGATGGTCGACCCGTGAAAGAAATATAGCTTCAGCTGATTTGTCTGCAGTGTCCAAGGCTATCGAATCTGGTTTAGTACCTGTTTTGCATGGAGATGCTGTGCTCGATGAGTTACTGGACTGTACCATATTGAGTGGAGATGTCATCATTAGTCACCTTGCAGCGCACTTGAAGCCTGACTTTGTTGTTTTTCTCACTGATGTTTTTGGGGTTTATGACCGTCCACCGTCGGAACCAAATGCAGTACTCTTGAGAGAAATCGCTGTGGCTAAAGATGGGAGCTGGTCGATCGTGAGCCCAACACTTGAGAACATGAATAAGCAAGTTGAGACAGAAGCAGCAGCCCATGATACAACAGGCGGAATGCTGACCAAGATATCGGAAGCTGCCGCGATTGCAAAACTCGGAATCGATGTGTACATTGTGAAGGCAGCAACAACCCATGCTTTGAGGGCATTGAATGGAGAGTTGAAAGGCAGCATTCCAGATGACTGGCTTGGAACGGCAATCCGGTTTTCGAATGAATGA

Protein Analysis

342

Amino Acids

36.17

Weight (kDa)

5.42

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 98 - 308 5.9e-32 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 506
AccII CGCG 1 cut(s) 890
AciI CCGC 3 cut(s) 284, 858, 888
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 5 cut(s) 374, 576, 622, 744, 917
AfeI AGCGCT 1 cut(s) 34
AfiI CCNNNNNNNGG 2 cut(s) 388, 476
AgsI TTSAA 5 cut(s) 269, 338, 670, 958, 970
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 7 cut(s) 163, 341, 427, 527, 533, 780, 884
AluI AGCT 7 cut(s) 163, 341, 427, 527, 533, 780, 884
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 4 cut(s) 2, 116, 489, 821
Aor51HI AGCGCT 1 cut(s) 34
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 9 cut(s) 63, 95, 160, 659, 836, 839, 884, 929, 975
ApoI RAATTY 1 cut(s) 243
AspLEI GCGC 2 cut(s) 35, 664
AspS9I GGNCC 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 644
AsuII TTCGAA 1 cut(s) 1016
AvaII GGWCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 797
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 603
BbvCI CCTCAGC 1 cut(s) 164
BbvI GCAGC 9 cut(s) 50, 107, 172, 671, 848, 851, 871, 941, 987
BccI CCATC 4 cut(s) 142, 205, 495, 767
BceAI ACGGC 1 cut(s) 1017
BciT130I CCWGG 1 cut(s) 231
BciVI GTATCC 1 cut(s) 476
BcoDI GTCTC 4 cut(s) 2, 116, 489, 821
BfaI CTAG 3 cut(s) 86, 342, 434
BfmI CTRYAG 1 cut(s) 543
BfoI RGCGCY 1 cut(s) 36
BfuAI ACCTGC 1 cut(s) 308
BfuI GTATCC 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 744
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 2 cut(s) 288, 732
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 3 cut(s) 75, 420, 583
BpiI GAAGAC 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 1016
BpuEI CTTGAG 3 cut(s) 128, 769, 824
Bsa29I ATCGAT 1 cut(s) 909
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 1 cut(s) 552
BsaWI WCCGGW 1 cut(s) 1008
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 476
Bse1I ACTGG 3 cut(s) 278, 618, 995
BseBI CCWGG 1 cut(s) 231
BseCI ATCGAT 1 cut(s) 909
BseDI CCNNGG 1 cut(s) 552
BseGI GGATG 2 cut(s) 216, 506
BseLI CCNNNNNNNGG 2 cut(s) 388, 476
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 3 cut(s) 278, 618, 995
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 9 cut(s) 50, 107, 172, 671, 848, 851, 871, 941, 987
BsgI GTGCAG 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 890
Bsh1285I CGRYCG 1 cut(s) 789
BshFI GGCC 1 cut(s) 277
BshVI ATCGAT 1 cut(s) 909
BsiEI CGRYCG 1 cut(s) 789
BsiHKAI GWGCWC 1 cut(s) 603
BsiSI CCGG 1 cut(s) 1009
BslI CCNNNNNNNGG 2 cut(s) 388, 476
BsmAI GTCTC 4 cut(s) 2, 116, 489, 821
BsmI GAATGC 2 cut(s) 867, 978
BsnI GGCC 1 cut(s) 277
Bso31I GGTCTC 1 cut(s) 2
Bsp119I TTCGAA 1 cut(s) 1016
Bsp1286I GDGCHC 2 cut(s) 603, 797
Bsp1407I TGTACA 1 cut(s) 915
Bsp143I GATC 2 cut(s) 22, 786
BspACI CCGC 3 cut(s) 284, 858, 888
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 1 cut(s) 156
BspDI ATCGAT 1 cut(s) 909
BspFNI CGCG 1 cut(s) 890
BspLI GGNNCC 2 cut(s) 288, 732
BspMAI CTGCAG 1 cut(s) 547
BspMI ACCTGC 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 1016
BspTNI GGTCTC 1 cut(s) 2
BsrGI TGTACA 1 cut(s) 915
BsrI ACTGG 3 cut(s) 278, 618, 995
BssECI CCNNGG 1 cut(s) 552
BssMI GATC 2 cut(s) 22, 786
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 3 cut(s) 620, 719, 726
BstAUI TGTACA 1 cut(s) 915
BstBI TTCGAA 1 cut(s) 1016
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 386
BstF5I GGATG 2 cut(s) 216, 506
BstFNI CGCG 1 cut(s) 890
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 664
BstKTI GATC 2 cut(s) 25, 789
BstMAI GTCTC 4 cut(s) 2, 116, 489, 821
BstMBI GATC 2 cut(s) 22, 786
BstMCI CGRYCG 1 cut(s) 789
BstMWI GCNNNNNNNGC 4 cut(s) 166, 670, 893, 950
BstNI CCWGG 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 1 cut(s) 890
BstV1I GCAGC 9 cut(s) 50, 107, 172, 671, 848, 851, 871, 941, 987
BstV2I GAAGAC 1 cut(s) 74
BstXI CCANNNNNNTGG 1 cut(s) 990
Bsu15I ATCGAT 1 cut(s) 909
BsuI GTATCC 1 cut(s) 476
BsuRI GGCC 1 cut(s) 277
BsuTUI ATCGAT 1 cut(s) 909
BtsCI GGATG 2 cut(s) 216, 506
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 552, 693
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 224
CfoI GCGC 2 cut(s) 35, 664
Cfr13I GGNCC 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 909
Csp6I GTAC 5 cut(s) 373, 575, 621, 743, 916
CviAII CATG 6 cut(s) 311, 495, 587, 811, 845, 941
CviQI GTAC 5 cut(s) 373, 575, 621, 743, 916
DdeI CTNAG 1 cut(s) 164
DpnI GATC 2 cut(s) 24, 788
DpnII GATC 2 cut(s) 22, 786
EaeI YGGCCR 1 cut(s) 275
EciI GGCGGA 1 cut(s) 873
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 797
Eco31I GGTCTC 1 cut(s) 2
Eco32I GATATC 1 cut(s) 876
Eco47I GGWCC 1 cut(s) 286
Eco47III AGCGCT 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 513
EcoNI CCTNNNNNAGG 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 229
EcoRV GATATC 1 cut(s) 876
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 1 cut(s) 797
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 6 cut(s) 314, 498, 590, 814, 848, 944
FalI AAGNNNNNCTT 2 cut(s) 662, 694
FatI CATG 6 cut(s) 310, 494, 586, 810, 844, 940
FblI GTMKAC 1 cut(s) 506
FokI GGATG 2 cut(s) 223, 513
FriOI GRGCYC 1 cut(s) 797
FspBI CTAG 3 cut(s) 86, 342, 434
GlaI GCGC 2 cut(s) 34, 663
GsuI CTGGAG 1 cut(s) 252
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 1009
HhaI GCGC 2 cut(s) 35, 664
Hin1II CATG 6 cut(s) 314, 498, 590, 814, 848, 944
Hin6I GCGC 2 cut(s) 33, 662
HinP1I GCGC 2 cut(s) 33, 662
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 4 cut(s) 58, 236, 563, 906
HpaII CCGG 1 cut(s) 1009
HphI GGTGA 1 cut(s) 644
Hpy166II GTNNAC 3 cut(s) 507, 722, 916
Hpy188I TCNGA 4 cut(s) 241, 730, 880, 905
Hpy188III TCNNGA 6 cut(s) 49, 145, 434, 748, 790, 983
Hpy8I GTNNAC 3 cut(s) 507, 722, 916
Hpy99I CGWCG 2 cut(s) 160, 730
HpyAV CCTTC 3 cut(s) 201, 480, 919
HpyCH4III ACNGT 3 cut(s) 620, 719, 726
HpyCH4V TGCA 8 cut(s) 66, 95, 108, 545, 586, 659, 740, 896
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 670, 893, 950
HpyF3I CTNAG 1 cut(s) 164
Hsp92II CATG 6 cut(s) 314, 498, 590, 814, 848, 944
HspAI GCGC 2 cut(s) 33, 662
Kzo9I GATC 2 cut(s) 22, 786
LmnI GCTCC 3 cut(s) 219, 314, 777
Lsp1109I GCAGC 9 cut(s) 50, 107, 172, 671, 848, 851, 871, 941, 987
LweI GCATC 3 cut(s) 75, 420, 583
MaeI CTAG 3 cut(s) 86, 342, 434
MaeIII GTNAC 4 cut(s) 100, 418, 609, 650
MalI GATC 2 cut(s) 24, 788
MboI GATC 2 cut(s) 22, 786
MboII GAAGA 1 cut(s) 74
MhlI GDGCHC 2 cut(s) 603, 797
MluCI AATT 3 cut(s) 116, 243, 438
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 6 cut(s) 56, 83, 159, 193, 454, 942
MslI CAYNNNNRTG 5 cut(s) 363, 479, 493, 499, 591
MspA1I CMGCKG 2 cut(s) 163, 533
MspI CCGG 1 cut(s) 1009
MspR9I CCNGG 1 cut(s) 231
Mva1269I GAATGC 2 cut(s) 867, 978
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 890
MwoI GCNNNNNNNGC 4 cut(s) 166, 670, 893, 950
NdeII GATC 2 cut(s) 22, 786
NlaIII CATG 6 cut(s) 314, 498, 590, 814, 848, 944
NlaIV GGNNCC 2 cut(s) 288, 732
NmuCI GTSAC 1 cut(s) 650
NspV TTCGAA 1 cut(s) 1016
PctI GAATGC 2 cut(s) 867, 978
PfeI GAWTC 4 cut(s) 58, 236, 563, 906
Ple19I CGATCG 1 cut(s) 789
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 288, 732
PspPI GGNCC 1 cut(s) 286
PstI CTGCAG 1 cut(s) 547
PvuI CGATCG 1 cut(s) 789
PvuII CAGCTG 2 cut(s) 163, 533
RsaI GTAC 5 cut(s) 374, 576, 622, 744, 917
RsaNI GTAC 5 cut(s) 373, 575, 621, 743, 916
RseI CAYNNNNRTG 5 cut(s) 363, 479, 493, 499, 591
SalI GTCGAC 1 cut(s) 505
Sau3AI GATC 2 cut(s) 22, 786
Sau96I GGNCC 1 cut(s) 286
ScaI AGTACT 1 cut(s) 744
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 2 cut(s) 603, 797
SfaNI GCATC 3 cut(s) 75, 420, 583
SfcI CTRYAG 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 1016
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 5 cut(s) 363, 479, 493, 499, 591
SmlI CTYRAG 3 cut(s) 143, 748, 803
SmoI CTYRAG 3 cut(s) 143, 748, 803
Sse9I AATT 3 cut(s) 116, 243, 438
SsiI CCGC 3 cut(s) 284, 858, 888
SspMI CTAG 3 cut(s) 86, 342, 434
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 3 cut(s) 620, 719, 726
TaqI TCGA 6 cut(s) 506, 561, 603, 785, 909, 1016
TaqII GACCGA 1 cut(s) 68
TasI AATT 3 cut(s) 116, 243, 438
TatI WGTACW 2 cut(s) 742, 915
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 4 cut(s) 58, 236, 563, 906
TscAI CASTG 2 cut(s) 552, 700
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 9 cut(s) 63, 95, 160, 659, 836, 839, 884, 929, 975
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 6 cut(s) 147, 237, 299, 347, 483, 827
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 2 cut(s) 552, 700
VpaK11BI GGWCC 1 cut(s) 286
XagI CCTNNNNNAGG 1 cut(s) 386
XapI RAATTY 1 cut(s) 243
XbaI TCTAGA 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 506
XspI CTAG 3 cut(s) 86, 342, 434
ZrmI AGTACT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.