Rw0G016960

Amino acid kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0008655
Physical Location & Seq
Reverse (-)
628453 .. 630964
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016960.1

Sequence Viewer

Length: 1020 bp
ATGGAGGAGAAGAGTGACAAAACTCTGCAACAAAGCGGTTCTGTTCATCCCACAAAATCGATTCGTTGCATCGTCAAACTCGGAGGTGCAGCAATTACTTGCAAGAAAGAATTAGAGACCATAAATGAAGAGAATCTCAAGATGGTTTCCTCGCAGCTGAGGCAATCGATGATTGCTGGTTCGTCTTCTGGAAAGGTTCTTGGGATGGATTGGAGCAAGAAATCTGGAGAACCAGAAATTTCATTCACTGTGGATGATTTCGAAGATCAACCAGTTGCAGAGTCGAACCCTTTTGTAGTTGTTCATGGAGCAGGCTCTTTTGGGCACTTTCAAGCTAGTAAATCTGGGGTGCATAAAGGAGGATGGAATCGACCCCTGGTCAAGGCTGGTTTTGTTGCCACCCGCATATCTGTTACGAATCTCAATCTTGAAATTGTTAGAGCCCTAGCCAGAGAGGGGATTCCTTCCATAGGAATGTCTCCATTTGCATGTGGATGGTCAACTAGCGAAAGGAATGTAGCCTCAGCTGATTTGTCTGTAGTGACCAAGGCTATTGAATCTGGTTTAGTACCTGTTCTGCATGGAGATGCTGTGCTCGATGACTTACTGGACTGCACCATATTGAGTGGGGATGTTATCATCAGTCATCTAGCAGCACACTTGAAGCCTGACTACGTTGTTTTTCTTACGGATGTTCTCGGGGTATATGATCGTCCACCATCAGAACCCAATGCAATACTCTTGAGAGAAATTGCTGTGGCTGAAGATGGGAGCTGGTCCGTTGTCAGGCCAACACTTCAGGACATGAATACTCGAGTTAAAACTAGTGTAGCAGCCCATGATACAACTGGTGGAATGATGACCAAGATAGGAGAAGCTGCAATGATTGCAAAGCTAGGGATCAATGTCTATATTGTGAAGGCAGCCACAAGCCATGCGTTGAGGGCCTTGAATGGTGAATTGCAAGGCGAAATTCCTGATGATTGGCTTGGAACATTAATCCGGTTTTCAAGTAAATGA

Protein Analysis

339

Amino Acids

36.25

Weight (kDa)

5.87

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 22 - 306 9.7e-34 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 36, 403
AclWI GGATC 1 cut(s) 908
AcsI RAATTY 2 cut(s) 237, 972
AcuI CTGAAG 2 cut(s) 782, 783
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 4 cut(s) 382, 456, 470, 786
AgsI TTSAA 6 cut(s) 332, 431, 557, 664, 952, 1011
AhdI GACNNNNNGTC 1 cut(s) 377
AhlI ACTAGT 1 cut(s) 824
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 6 cut(s) 157, 335, 527, 774, 878, 895
AluI AGCT 6 cut(s) 157, 335, 527, 774, 878, 895
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 2 cut(s) 110, 483
AlwI GGATC 1 cut(s) 908
Ama87I CYCGRG 2 cut(s) 698, 813
AoxI GGCC 2 cut(s) 788, 945
ApeKI GCWGC 6 cut(s) 89, 154, 653, 833, 878, 923
ApoI RAATTY 2 cut(s) 237, 972
AseI ATTAAT 1 cut(s) 998
AspS9I GGNCC 2 cut(s) 777, 945
AsuHPI GGTGA 1 cut(s) 968
AsuII TTCGAA 1 cut(s) 261
AvaI CYCGRG 2 cut(s) 698, 813
AvaII GGWCC 1 cut(s) 777
BaeGI GKGCMC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 445
BarI GAAGNNNNNNTAC 2 cut(s) 656, 688
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 597
BbvCI CCTCAGC 2 cut(s) 158, 523
BbvI GCAGC 6 cut(s) 101, 166, 665, 845, 865, 935
BccI CCATC 6 cut(s) 136, 199, 357, 489, 727, 761
BciT130I CCWGG 1 cut(s) 377
BcoDI GTCTC 2 cut(s) 110, 483
BcuI ACTAGT 1 cut(s) 824
BfaI CTAG 6 cut(s) 336, 446, 504, 650, 825, 896
BfmI CTRYAG 1 cut(s) 537
BisI GCNGC 6 cut(s) 90, 155, 654, 834, 879, 924
BlsI GCNGC 6 cut(s) 91, 156, 655, 835, 880, 925
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 1 cut(s) 777
BmeRI GACNNNNNGTC 1 cut(s) 377
BmeT110I CYCGRG 2 cut(s) 698, 813
BmgT120I GGNCC 2 cut(s) 777, 945
BmrFI CCNGG 1 cut(s) 377
BmsI GCATC 2 cut(s) 78, 577
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 2 cut(s) 158, 523
Bpu14I TTCGAA 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 122, 763
Bsa29I ATCGAT 2 cut(s) 59, 167
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 2 cut(s) 375, 546
BsaWI WCCGGW 1 cut(s) 1002
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 456, 470, 786
Bse1I ACTGG 3 cut(s) 272, 612, 853
Bse3DI GCAATG 1 cut(s) 888
BseBI CCWGG 1 cut(s) 377
BseCI ATCGAT 2 cut(s) 59, 167
BseDI CCNNGG 2 cut(s) 375, 546
BseGI GGATG 7 cut(s) 46, 210, 259, 368, 500, 637, 697
BseLI CCNNNNNNNGG 4 cut(s) 382, 456, 470, 786
BseMI GCAATG 1 cut(s) 888
BseMII CTCAG 2 cut(s) 149, 537
BseNI ACTGG 3 cut(s) 272, 612, 853
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 6 cut(s) 101, 166, 665, 845, 865, 935
BsgI GTGCAG 2 cut(s) 108, 598
BshFI GGCC 2 cut(s) 790, 947
BshVI ATCGAT 2 cut(s) 59, 167
BsiHKAI GWGCWC 1 cut(s) 597
BsiHKCI CYCGRG 2 cut(s) 698, 813
BsiSI CCGG 1 cut(s) 1003
BslI CCNNNNNNNGG 4 cut(s) 382, 456, 470, 786
BsmAI GTCTC 2 cut(s) 110, 483
BsnI GGCC 2 cut(s) 790, 947
Bso31I GGTCTC 1 cut(s) 110
BsoBI CYCGRG 2 cut(s) 698, 813
Bsp119I TTCGAA 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 327, 445, 597
Bsp143I GATC 3 cut(s) 265, 709, 900
BspACI CCGC 2 cut(s) 36, 403
BspANI GGCC 2 cut(s) 790, 947
BspCNI CTCAG 2 cut(s) 150, 536
BspDI ATCGAT 2 cut(s) 59, 167
BspPI GGATC 1 cut(s) 908
BspT104I TTCGAA 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 888
BsrI ACTGG 3 cut(s) 272, 612, 853
BssECI CCNNGG 2 cut(s) 375, 546
BssMI GATC 3 cut(s) 265, 709, 900
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 5, 123
BstAPI GCANNNNNTGC 1 cut(s) 887
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 2 cut(s) 158, 523
BstENI CCTNNNNNAGG 2 cut(s) 380, 468
BstF5I GGATG 7 cut(s) 46, 210, 259, 368, 500, 637, 697
BstKTI GATC 3 cut(s) 268, 712, 903
BstMAI GTCTC 2 cut(s) 110, 483
BstMBI GATC 3 cut(s) 265, 709, 900
BstMWI GCNNNNNNNGC 3 cut(s) 160, 887, 944
BstNI CCWGG 1 cut(s) 377
BstNSI RCATGY 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 375
BstSFI CTRYAG 1 cut(s) 537
BstSLI GKGCMC 1 cut(s) 327
BstV1I GCAGC 6 cut(s) 101, 166, 665, 845, 865, 935
BstV2I GAAGAC 1 cut(s) 177
Bsu15I ATCGAT 2 cut(s) 59, 167
BsuRI GGCC 2 cut(s) 790, 947
BsuTUI ATCGAT 2 cut(s) 59, 167
BtsCI GGATG 7 cut(s) 46, 210, 259, 368, 500, 637, 697
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 777, 945
ClaI ATCGAT 2 cut(s) 59, 167
Csp6I GTAC 1 cut(s) 569
CviAII CATG 6 cut(s) 305, 489, 581, 805, 839, 935
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 2 cut(s) 158, 523
DpnI GATC 3 cut(s) 267, 711, 902
DpnII GATC 3 cut(s) 265, 709, 900
DriI GACNNNNNGTC 1 cut(s) 377
Eam1104I CTCTTC 2 cut(s) 5, 123
Eam1105I GACNNNNNGTC 1 cut(s) 377
EarI CTCTTC 2 cut(s) 5, 123
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 445
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 777
Eco57I CTGAAG 2 cut(s) 782, 783
Eco88I CYCGRG 2 cut(s) 698, 813
EcoNI CCTNNNNNAGG 2 cut(s) 380, 468
EcoO109I RGGNCCY 1 cut(s) 945
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 445
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 6 cut(s) 308, 492, 584, 808, 842, 938
FatI CATG 6 cut(s) 304, 488, 580, 804, 838, 934
FauI CCCGC 1 cut(s) 410
Fnu4HI GCNGC 6 cut(s) 90, 155, 654, 834, 879, 924
FokI GGATG 7 cut(s) 33, 217, 266, 375, 507, 644, 704
FriOI GRGCYC 1 cut(s) 445
Fsp4HI GCNGC 6 cut(s) 90, 155, 654, 834, 879, 924
FspBI CTAG 6 cut(s) 336, 446, 504, 650, 825, 896
GluI GCNGC 6 cut(s) 90, 155, 654, 834, 879, 924
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 2 cut(s) 790, 947
HapII CCGG 1 cut(s) 1003
Hin1II CATG 6 cut(s) 308, 492, 584, 808, 842, 938
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HinfI GANTC 7 cut(s) 61, 133, 281, 367, 418, 460, 557
HpaII CCGG 1 cut(s) 1003
HphI GGTGA 1 cut(s) 968
Hpy166II GTNNAC 2 cut(s) 501, 716
Hpy188I TCNGA 2 cut(s) 83, 724
Hpy188III TCNNGA 7 cut(s) 139, 189, 225, 428, 742, 800, 977
Hpy8I GTNNAC 2 cut(s) 501, 716
HpyAV CCTTC 2 cut(s) 474, 913
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 675
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 887, 944
HpyF3I CTNAG 2 cut(s) 158, 523
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 6 cut(s) 308, 492, 584, 808, 842, 938
Kzo9I GATC 3 cut(s) 265, 709, 900
LmnI GCTCC 3 cut(s) 213, 308, 771
Lsp1109I GCAGC 6 cut(s) 101, 166, 665, 845, 865, 935
LweI GCATC 2 cut(s) 78, 577
MaeI CTAG 6 cut(s) 336, 446, 504, 650, 825, 896
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 3 cut(s) 14, 412, 541
MalI GATC 3 cut(s) 267, 711, 902
MboI GATC 3 cut(s) 265, 709, 900
MboII GAAGA 5 cut(s) 22, 140, 177, 275, 776
MhlI GDGCHC 3 cut(s) 327, 445, 597
MluCI AATT 7 cut(s) 93, 110, 237, 432, 750, 959, 972
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 7 cut(s) 77, 153, 160, 353, 448, 532, 936
MseI TTAA 2 cut(s) 819, 998
MslI CAYNNNNRTG 4 cut(s) 473, 487, 493, 585
MspA1I CMGCKG 2 cut(s) 157, 527
MspI CCGG 1 cut(s) 1003
MspR9I CCNGG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 160, 887, 944
NdeII GATC 3 cut(s) 265, 709, 900
NlaIII CATG 6 cut(s) 308, 492, 584, 808, 842, 938
NmuCI GTSAC 2 cut(s) 14, 541
NspI RCATGY 1 cut(s) 492
NspV TTCGAA 1 cut(s) 261
PaeR7I CTCGAG 1 cut(s) 813
PfeI GAWTC 6 cut(s) 61, 133, 367, 418, 460, 557
PkrI GCNGC 6 cut(s) 91, 156, 655, 835, 880, 925
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 998
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspPI GGNCC 2 cut(s) 777, 945
PspXI VCTCGAGB 1 cut(s) 813
PvuII CAGCTG 2 cut(s) 157, 527
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 4 cut(s) 473, 487, 493, 585
SaqAI TTAA 2 cut(s) 819, 998
SatI GCNGC 6 cut(s) 90, 155, 654, 834, 879, 924
Sau3AI GATC 3 cut(s) 265, 709, 900
Sau96I GGNCC 2 cut(s) 777, 945
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 3 cut(s) 327, 445, 597
SfaNI GCATC 2 cut(s) 78, 577
SfcI CTRYAG 1 cut(s) 537
Sfr274I CTCGAG 1 cut(s) 813
SfuI TTCGAA 1 cut(s) 261
SinI GGWCC 1 cut(s) 777
SlaI CTCGAG 1 cut(s) 813
SmiMI CAYNNNNRTG 4 cut(s) 473, 487, 493, 585
SmlI CTYRAG 3 cut(s) 137, 742, 813
SmoI CTYRAG 3 cut(s) 137, 742, 813
SpeI ACTAGT 1 cut(s) 824
Sse9I AATT 7 cut(s) 93, 110, 237, 432, 750, 959, 972
SsiI CCGC 2 cut(s) 36, 403
SspMI CTAG 6 cut(s) 336, 446, 504, 650, 825, 896
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 678
TaqI TCGA 7 cut(s) 59, 167, 261, 284, 370, 597, 814
TasI AATT 7 cut(s) 93, 110, 237, 432, 750, 959, 972
TfiI GAWTC 6 cut(s) 61, 133, 367, 418, 460, 557
Tru1I TTAA 2 cut(s) 819, 998
Tru9I TTAA 2 cut(s) 819, 998
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 2 cut(s) 14, 541
TseI GCWGC 6 cut(s) 89, 154, 653, 833, 878, 923
Tsp45I GTSAC 2 cut(s) 14, 541
TspDTI ATGAA 5 cut(s) 35, 141, 231, 293, 821
TspGWI ACGGA 2 cut(s) 704, 769
TspRI CASTG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 777
VspI ATTAAT 1 cut(s) 998
XagI CCTNNNNNAGG 2 cut(s) 380, 468
XapI RAATTY 2 cut(s) 237, 972
XceI RCATGY 1 cut(s) 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 845
XhoI CTCGAG 1 cut(s) 813
XspI CTAG 6 cut(s) 336, 446, 504, 650, 825, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.