Rh4DG371800

Amino acid kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008655
Physical Location & Seq
Reverse (-)
59013620 .. 59017043
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG371800.1

Sequence Viewer

Length: 765 bp
ATGGAGGAGAAGAGTGACAAAACCGAACAAAGCGGTTCTGTTCATCTCACAAAATCGATTCGTTGCATCGTCAAACTCGGAGGTGCAGCAATTACTTGCAAGAATGAATTAGAGACCATAAATGAAGAGAATCTCAAGATGGTTTCCTCGCAGCTGAGGCAATCGATGATTGCTGGTTCGTCTTCTGGAAAGGTTCTTGGGATGGATTGGAGCAAGAAATCTGGAGAACCAGAAATTTCATTCACTGTGGATGATTTCGAAGATCAACCAGTTGCGGAGTCGAGCCCTTTTGTAGTTGTTCATGGAGCAGGCTCTTTTGGGCACTTTCAAGCTAGTAAATCTGGGGTGCATAAAGGAGGATGGAATCGACCCCTGGTCAAGGCTGGTTTTGTTGCCACCCGCATATCTGTTACGAATCTCAATCTCGAAATTGTTAGAGCCCTAGCCAGAGAGGGGATTCCTTCCATAGGAATGTCTCCATTTGCATGTGGATGGTCAACTAGCGAAAGGAATGTAGCCTCAGCTGATTTGTCTGTAGTGACCAAGGCTATTGAATCTGGTTTAGTACCTGTTCTACATGGAGATGCTGTGCTCGATGACTTACTGGACTGCACCATATTGAGTGGGGATGTTATCATCAGTCATCTAGCAGCACACTTGAAGCCTGACTACGTTGTTTTTCTTACGGATGTTCTCGGGGTATATGATCGTCCACCATCAGAACCCAATGCAATACTCTTGAGAGAAATTGGTAAGGTCAACTGA

Protein Analysis

254

Amino Acids

27.14

Weight (kDa)

5.52

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 21 - 250 1.3e-27 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 33, 275, 400
AcsI RAATTY 1 cut(s) 234
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 3 cut(s) 379, 453, 467
AgsI TTSAA 3 cut(s) 329, 554, 661
AhdI GACNNNNNGTC 1 cut(s) 374
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 3 cut(s) 154, 332, 524
AluI AGCT 3 cut(s) 154, 332, 524
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 2 cut(s) 107, 480
Ama87I CYCGRG 1 cut(s) 695
ApeKI GCWGC 3 cut(s) 86, 151, 650
ApoI RAATTY 1 cut(s) 234
AsuII TTCGAA 1 cut(s) 258
AvaI CYCGRG 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 324
BanII GRGCYC 2 cut(s) 287, 442
BarI GAAGNNNNNNTAC 2 cut(s) 653, 685
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 594
BbvCI CCTCAGC 2 cut(s) 155, 520
BbvI GCAGC 3 cut(s) 98, 163, 662
BccI CCATC 5 cut(s) 133, 196, 354, 486, 724
BciT130I CCWGG 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 107, 480
BfaI CTAG 4 cut(s) 333, 443, 501, 647
BfmI CTRYAG 1 cut(s) 534
BisI GCNGC 3 cut(s) 87, 152, 651
BlsI GCNGC 3 cut(s) 88, 153, 652
Bme1390I CCNGG 1 cut(s) 374
BmeRI GACNNNNNGTC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 695
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 2 cut(s) 75, 574
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 2 cut(s) 155, 520
Bpu14I TTCGAA 1 cut(s) 258
BpuEI CTTGAG 2 cut(s) 119, 760
Bsa29I ATCGAT 2 cut(s) 56, 164
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 372, 543
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 379, 453, 467
Bse1I ACTGG 2 cut(s) 269, 609
BseBI CCWGG 1 cut(s) 374
BseCI ATCGAT 2 cut(s) 56, 164
BseDI CCNNGG 2 cut(s) 372, 543
BseGI GGATG 6 cut(s) 207, 256, 365, 497, 634, 694
BseLI CCNNNNNNNGG 3 cut(s) 379, 453, 467
BseMII CTCAG 2 cut(s) 146, 534
BseNI ACTGG 2 cut(s) 269, 609
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 3 cut(s) 98, 163, 662
BsgI GTGCAG 2 cut(s) 105, 595
BshVI ATCGAT 2 cut(s) 56, 164
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 695
BslI CCNNNNNNNGG 3 cut(s) 379, 453, 467
BsmAI GTCTC 2 cut(s) 107, 480
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 695
Bsp119I TTCGAA 1 cut(s) 258
Bsp1286I GDGCHC 4 cut(s) 287, 324, 442, 594
Bsp143I GATC 2 cut(s) 262, 706
BspACI CCGC 3 cut(s) 33, 275, 400
BspCNI CTCAG 2 cut(s) 147, 533
BspDI ATCGAT 2 cut(s) 56, 164
BspT104I TTCGAA 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 2 cut(s) 269, 609
BssECI CCNNGG 2 cut(s) 372, 543
BssMI GATC 2 cut(s) 262, 706
BssT1I CCWWGG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 2 cut(s) 5, 120
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 2 cut(s) 155, 520
BstENI CCTNNNNNAGG 2 cut(s) 377, 465
BstF5I GGATG 6 cut(s) 207, 256, 365, 497, 634, 694
BstKTI GATC 2 cut(s) 265, 709
BstMAI GTCTC 2 cut(s) 107, 480
BstMBI GATC 2 cut(s) 262, 706
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 324
BstV1I GCAGC 3 cut(s) 98, 163, 662
BstV2I GAAGAC 1 cut(s) 174
Bsu15I ATCGAT 2 cut(s) 56, 164
BsuTUI ATCGAT 2 cut(s) 56, 164
BtsCI GGATG 6 cut(s) 207, 256, 365, 497, 634, 694
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 310
ClaI ATCGAT 2 cut(s) 56, 164
Csp6I GTAC 1 cut(s) 566
CviAII CATG 3 cut(s) 302, 486, 578
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 2 cut(s) 155, 520
DpnI GATC 2 cut(s) 264, 708
DpnII GATC 2 cut(s) 262, 706
DriI GACNNNNNGTC 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 5, 120
Eam1105I GACNNNNNGTC 1 cut(s) 374
EarI CTCTTC 2 cut(s) 5, 120
Eco130I CCWWGG 1 cut(s) 543
Eco24I GRGCYC 2 cut(s) 287, 442
Eco31I GGTCTC 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 695
EcoNI CCTNNNNNAGG 2 cut(s) 377, 465
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 543
EcoT38I GRGCYC 2 cut(s) 287, 442
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 3 cut(s) 305, 489, 581
FatI CATG 3 cut(s) 301, 485, 577
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 3 cut(s) 87, 152, 651
FokI GGATG 6 cut(s) 214, 263, 372, 504, 641, 701
FriOI GRGCYC 2 cut(s) 287, 442
Fsp4HI GCNGC 3 cut(s) 87, 152, 651
FspBI CTAG 4 cut(s) 333, 443, 501, 647
GluI GCNGC 3 cut(s) 87, 152, 651
GsuI CTGGAG 1 cut(s) 243
Hin1II CATG 3 cut(s) 305, 489, 581
HincII GTYRAC 2 cut(s) 498, 760
HindII GTYRAC 2 cut(s) 498, 760
HinfI GANTC 7 cut(s) 58, 130, 278, 364, 415, 457, 554
Hpy166II GTNNAC 3 cut(s) 498, 713, 760
Hpy188I TCNGA 2 cut(s) 80, 721
Hpy188III TCNNGA 5 cut(s) 136, 186, 222, 425, 739
Hpy8I GTNNAC 3 cut(s) 498, 713, 760
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 7 cut(s) 66, 86, 99, 349, 485, 612, 731
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 2 cut(s) 155, 520
HpySE526I ACGT 1 cut(s) 672
Hsp92II CATG 3 cut(s) 305, 489, 581
Kzo9I GATC 2 cut(s) 262, 706
LmnI GCTCC 2 cut(s) 210, 305
Lsp1109I GCAGC 3 cut(s) 98, 163, 662
LweI GCATC 2 cut(s) 75, 574
MaeI CTAG 4 cut(s) 333, 443, 501, 647
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 3 cut(s) 14, 409, 538
MalI GATC 2 cut(s) 264, 708
MboI GATC 2 cut(s) 262, 706
MboII GAAGA 4 cut(s) 22, 137, 174, 272
MhlI GDGCHC 4 cut(s) 287, 324, 442, 594
MluCI AATT 5 cut(s) 90, 107, 234, 429, 747
MlyI GAGTC 1 cut(s) 287
MnlI CCTC 6 cut(s) 74, 150, 157, 350, 445, 529
MslI CAYNNNNRTG 4 cut(s) 470, 484, 490, 582
MspA1I CMGCKG 2 cut(s) 154, 524
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 2 cut(s) 262, 706
NlaIII CATG 3 cut(s) 305, 489, 581
NmuCI GTSAC 2 cut(s) 14, 538
NspI RCATGY 1 cut(s) 489
NspV TTCGAA 1 cut(s) 258
PfeI GAWTC 6 cut(s) 58, 130, 364, 415, 457, 554
PkrI GCNGC 3 cut(s) 88, 153, 652
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PvuII CAGCTG 2 cut(s) 154, 524
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
RseI CAYNNNNRTG 4 cut(s) 470, 484, 490, 582
SatI GCNGC 3 cut(s) 87, 152, 651
Sau3AI GATC 2 cut(s) 262, 706
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 374
SduI GDGCHC 4 cut(s) 287, 324, 442, 594
SetI ASST 8 cut(s) 85, 156, 195, 334, 526, 571, 675, 759
SfaNI GCATC 2 cut(s) 75, 574
SfcI CTRYAG 1 cut(s) 534
SfuI TTCGAA 1 cut(s) 258
SmiMI CAYNNNNRTG 4 cut(s) 470, 484, 490, 582
SmlI CTYRAG 2 cut(s) 134, 739
SmoI CTYRAG 2 cut(s) 134, 739
Sse9I AATT 5 cut(s) 90, 107, 234, 429, 747
SsiI CCGC 3 cut(s) 33, 275, 400
SspMI CTAG 4 cut(s) 333, 443, 501, 647
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 675
TaqI TCGA 7 cut(s) 56, 164, 258, 281, 367, 426, 594
TasI AATT 5 cut(s) 90, 107, 234, 429, 747
TfiI GAWTC 6 cut(s) 58, 130, 364, 415, 457, 554
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 2 cut(s) 14, 538
TseI GCWGC 3 cut(s) 86, 151, 650
Tsp45I GTSAC 2 cut(s) 14, 538
TspDTI ATGAA 5 cut(s) 32, 120, 138, 228, 290
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 1 cut(s) 250
XagI CCTNNNNNAGG 2 cut(s) 377, 465
XapI RAATTY 1 cut(s) 234
XceI RCATGY 1 cut(s) 489
XspI CTAG 4 cut(s) 333, 443, 501, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.