Rh4AG365800

Amino acid kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008655
Physical Location & Seq
Reverse (-)
67357534 .. 67360068
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG365800.1

Sequence Viewer

Length: 1017 bp
ATGGAGGAGAAGAGTGACAAAACCGAACAAAGCGGTTCTGTTCATCTCACAAAATCGATTCGTTGCATCGTCAAACTCGGAGGTGCAGCAATTACTTGCAAGAATGAATTAGAGACCATAAATGAAGAGAATCTCAAGATGGTTTCCTCGCAGCTGAGGCAATCGATGATTGCTGGTTCGTCTTCTGGAAAGGTTCTTGGGATGGATTGGAGCAAGAAATCTGGAGAACCAGAAATTTCATTCACTGTGGATGATTTCGAAGATCAACCAGTTGCGGAGTCGAGCCCTTTTGTAGTTGTTCATGGAGCAGGCTCTTTTGGGCACTTTCAAGCTAGTAAATCTGGGGTGCATAAAGGAGGATGGAATCGACCCCTGGTCAAGGCTGGTTTTGTTGCCACCCGCATATCTGTTACGAATCTCAATCTCGAAATTGTTAGAGCCCTAGCCAGAGAGGGGATTCCTTCCATAGGAATGTCTCCATTTGCATGTGGATGGTCAACTAGCGAAAGGAATGTAGCCTCAGCTGATTTGTCTGTAGTGACCAAGGCTATTGAATCTGGTTTAGTACCTGTTCTACATGGAGATGCTGTGCTCGATGACTTACTGGACTGCACCATATTGAGTGGGGATGTTATCATCAGTCATCTAGCAGCACACTTGAAGCCTGACTACGTTGTTTTTCTTACGGATGTTCTCGGGGTATATGATCGTCCACCATCAGAACCCAATGCAATACTCTTGAGAGAAATTGCTGTGGCTGAAGATGGGAGCTGGTCCGTTGTGAGGCCAACACTTCAGGACATGAATACTCGAGTTAAAACTAGTGTAGCAGCCCATGATACAACTGGTGGAATGATGACCAAGATAGGAGAAGCTGCAATGATTGCAAAGCTAGGGATCAATGTCTATATTGTGAAGGCAGCCACAAGCCATGCATTGAGGGCCTTGAGCGGTGAATTGCAAGGCGAATTTCCTGATGATTGGCTTGGAACATTAATCCGGTTTTCAAGTAAATGA

Protein Analysis

338

Amino Acids

36.12

Weight (kDa)

5.63

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 21 - 305 2.6e-33 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 951
AciI CCGC 4 cut(s) 33, 275, 400, 951
AclWI GGATC 1 cut(s) 905
AcsI RAATTY 2 cut(s) 234, 968
AcuI CTGAAG 2 cut(s) 779, 780
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 4 cut(s) 379, 453, 467, 783
AgsI TTSAA 4 cut(s) 329, 554, 661, 1008
AhdI GACNNNNNGTC 1 cut(s) 374
AhlI ACTAGT 1 cut(s) 821
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 6 cut(s) 154, 332, 524, 771, 875, 892
AluI AGCT 6 cut(s) 154, 332, 524, 771, 875, 892
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 2 cut(s) 107, 480
AlwI GGATC 1 cut(s) 905
Ama87I CYCGRG 2 cut(s) 695, 810
AoxI GGCC 2 cut(s) 785, 942
ApeKI GCWGC 6 cut(s) 86, 151, 650, 830, 875, 920
ApoI RAATTY 2 cut(s) 234, 968
AseI ATTAAT 1 cut(s) 995
AspS9I GGNCC 2 cut(s) 774, 942
AsuHPI GGTGA 1 cut(s) 965
AsuII TTCGAA 1 cut(s) 258
AvaI CYCGRG 2 cut(s) 695, 810
AvaII GGWCC 1 cut(s) 774
BaeGI GKGCMC 1 cut(s) 324
BanII GRGCYC 2 cut(s) 287, 442
BarI GAAGNNNNNNTAC 2 cut(s) 653, 685
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 594
BbvCI CCTCAGC 2 cut(s) 155, 520
BbvI GCAGC 6 cut(s) 98, 163, 662, 842, 862, 932
BccI CCATC 6 cut(s) 133, 196, 354, 486, 724, 758
BciT130I CCWGG 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 107, 480
BcuI ACTAGT 1 cut(s) 821
BfaI CTAG 6 cut(s) 333, 443, 501, 647, 822, 893
BfmI CTRYAG 1 cut(s) 534
BisI GCNGC 6 cut(s) 87, 152, 651, 831, 876, 921
BlsI GCNGC 6 cut(s) 88, 153, 652, 832, 877, 922
Bme1390I CCNGG 1 cut(s) 374
Bme18I GGWCC 1 cut(s) 774
BmeRI GACNNNNNGTC 1 cut(s) 374
BmeT110I CYCGRG 2 cut(s) 695, 810
BmgT120I GGNCC 2 cut(s) 774, 942
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 2 cut(s) 75, 574
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 2 cut(s) 155, 520
Bpu14I TTCGAA 1 cut(s) 258
BpuEI CTTGAG 3 cut(s) 119, 760, 967
Bsa29I ATCGAT 2 cut(s) 56, 164
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 372, 543
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 4 cut(s) 379, 453, 467, 783
Bse1I ACTGG 3 cut(s) 269, 609, 850
Bse3DI GCAATG 1 cut(s) 885
BseBI CCWGG 1 cut(s) 374
BseCI ATCGAT 2 cut(s) 56, 164
BseDI CCNNGG 2 cut(s) 372, 543
BseGI GGATG 6 cut(s) 207, 256, 365, 497, 634, 694
BseLI CCNNNNNNNGG 4 cut(s) 379, 453, 467, 783
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 2 cut(s) 146, 534
BseNI ACTGG 3 cut(s) 269, 609, 850
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 6 cut(s) 98, 163, 662, 842, 862, 932
BsgI GTGCAG 2 cut(s) 105, 595
BshFI GGCC 2 cut(s) 787, 944
BshVI ATCGAT 2 cut(s) 56, 164
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 2 cut(s) 695, 810
BsiSI CCGG 1 cut(s) 1000
BslI CCNNNNNNNGG 4 cut(s) 379, 453, 467, 783
BsmAI GTCTC 2 cut(s) 107, 480
BsnI GGCC 2 cut(s) 787, 944
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 2 cut(s) 695, 810
Bsp119I TTCGAA 1 cut(s) 258
Bsp1286I GDGCHC 4 cut(s) 287, 324, 442, 594
Bsp143I GATC 3 cut(s) 262, 706, 897
BspACI CCGC 4 cut(s) 33, 275, 400, 951
BspANI GGCC 2 cut(s) 787, 944
BspCNI CTCAG 2 cut(s) 147, 533
BspDI ATCGAT 2 cut(s) 56, 164
BspPI GGATC 1 cut(s) 905
BspT104I TTCGAA 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 951
BsrDI GCAATG 1 cut(s) 885
BsrI ACTGG 3 cut(s) 269, 609, 850
BssECI CCNNGG 2 cut(s) 372, 543
BssMI GATC 3 cut(s) 262, 706, 897
BssT1I CCWWGG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 2 cut(s) 5, 120
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 2 cut(s) 155, 520
BstENI CCTNNNNNAGG 2 cut(s) 377, 465
BstF5I GGATG 6 cut(s) 207, 256, 365, 497, 634, 694
BstKTI GATC 3 cut(s) 265, 709, 900
BstMAI GTCTC 2 cut(s) 107, 480
BstMBI GATC 3 cut(s) 262, 706, 897
BstMWI GCNNNNNNNGC 3 cut(s) 157, 884, 941
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 324
BstV1I GCAGC 6 cut(s) 98, 163, 662, 842, 862, 932
BstV2I GAAGAC 1 cut(s) 174
Bsu15I ATCGAT 2 cut(s) 56, 164
BsuRI GGCC 2 cut(s) 787, 944
BsuTUI ATCGAT 2 cut(s) 56, 164
BtsCI GGATG 6 cut(s) 207, 256, 365, 497, 634, 694
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 774, 942
ClaI ATCGAT 2 cut(s) 56, 164
Csp6I GTAC 1 cut(s) 566
CviAII CATG 6 cut(s) 302, 486, 578, 802, 836, 932
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 2 cut(s) 155, 520
DpnI GATC 3 cut(s) 264, 708, 899
DpnII GATC 3 cut(s) 262, 706, 897
DriI GACNNNNNGTC 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 5, 120
Eam1105I GACNNNNNGTC 1 cut(s) 374
EarI CTCTTC 2 cut(s) 5, 120
Eco130I CCWWGG 1 cut(s) 543
Eco24I GRGCYC 2 cut(s) 287, 442
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 774
Eco57I CTGAAG 2 cut(s) 779, 780
Eco88I CYCGRG 2 cut(s) 695, 810
EcoNI CCTNNNNNAGG 2 cut(s) 377, 465
EcoO109I RGGNCCY 1 cut(s) 942
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 543
EcoT22I ATGCAT 1 cut(s) 937
EcoT38I GRGCYC 2 cut(s) 287, 442
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 6 cut(s) 305, 489, 581, 805, 839, 935
FatI CATG 6 cut(s) 301, 485, 577, 801, 835, 931
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 6 cut(s) 87, 152, 651, 831, 876, 921
FokI GGATG 6 cut(s) 214, 263, 372, 504, 641, 701
FriOI GRGCYC 2 cut(s) 287, 442
Fsp4HI GCNGC 6 cut(s) 87, 152, 651, 831, 876, 921
FspBI CTAG 6 cut(s) 333, 443, 501, 647, 822, 893
GluI GCNGC 6 cut(s) 87, 152, 651, 831, 876, 921
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 2 cut(s) 787, 944
HapII CCGG 1 cut(s) 1000
Hin1II CATG 6 cut(s) 305, 489, 581, 805, 839, 935
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HinfI GANTC 7 cut(s) 58, 130, 278, 364, 415, 457, 554
HpaII CCGG 1 cut(s) 1000
HphI GGTGA 1 cut(s) 965
Hpy166II GTNNAC 2 cut(s) 498, 713
Hpy188I TCNGA 2 cut(s) 80, 721
Hpy188III TCNNGA 7 cut(s) 136, 186, 222, 425, 739, 797, 974
Hpy8I GTNNAC 2 cut(s) 498, 713
HpyAV CCTTC 2 cut(s) 471, 910
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 672
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 884, 941
HpyF3I CTNAG 2 cut(s) 155, 520
HpySE526I ACGT 1 cut(s) 672
Hsp92II CATG 6 cut(s) 305, 489, 581, 805, 839, 935
Kzo9I GATC 3 cut(s) 262, 706, 897
LmnI GCTCC 3 cut(s) 210, 305, 768
Lsp1109I GCAGC 6 cut(s) 98, 163, 662, 842, 862, 932
LweI GCATC 2 cut(s) 75, 574
MaeI CTAG 6 cut(s) 333, 443, 501, 647, 822, 893
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 3 cut(s) 14, 409, 538
MalI GATC 3 cut(s) 264, 708, 899
MbiI CCGCTC 1 cut(s) 951
MboI GATC 3 cut(s) 262, 706, 897
MboII GAAGA 5 cut(s) 22, 137, 174, 272, 773
MhlI GDGCHC 4 cut(s) 287, 324, 442, 594
MluCI AATT 7 cut(s) 90, 107, 234, 429, 747, 956, 968
MlyI GAGTC 1 cut(s) 287
MnlI CCTC 8 cut(s) 74, 150, 157, 350, 445, 529, 777, 933
Mph1103I ATGCAT 1 cut(s) 937
MseI TTAA 2 cut(s) 816, 995
MslI CAYNNNNRTG 4 cut(s) 470, 484, 490, 582
MspA1I CMGCKG 2 cut(s) 154, 524
MspI CCGG 1 cut(s) 1000
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 3 cut(s) 157, 884, 941
NdeII GATC 3 cut(s) 262, 706, 897
NlaIII CATG 6 cut(s) 305, 489, 581, 805, 839, 935
NmuCI GTSAC 2 cut(s) 14, 538
NsiI ATGCAT 1 cut(s) 937
NspI RCATGY 1 cut(s) 489
NspV TTCGAA 1 cut(s) 258
PaeR7I CTCGAG 1 cut(s) 810
PfeI GAWTC 6 cut(s) 58, 130, 364, 415, 457, 554
PkrI GCNGC 6 cut(s) 88, 153, 652, 832, 877, 922
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PshBI ATTAAT 1 cut(s) 995
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspPI GGNCC 2 cut(s) 774, 942
PspXI VCTCGAGB 1 cut(s) 810
PvuII CAGCTG 2 cut(s) 154, 524
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
RseI CAYNNNNRTG 4 cut(s) 470, 484, 490, 582
SaqAI TTAA 2 cut(s) 816, 995
SatI GCNGC 6 cut(s) 87, 152, 651, 831, 876, 921
Sau3AI GATC 3 cut(s) 262, 706, 897
Sau96I GGNCC 2 cut(s) 774, 942
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 374
SduI GDGCHC 4 cut(s) 287, 324, 442, 594
SfaNI GCATC 2 cut(s) 75, 574
SfcI CTRYAG 1 cut(s) 534
Sfr274I CTCGAG 1 cut(s) 810
SfuI TTCGAA 1 cut(s) 258
SinI GGWCC 1 cut(s) 774
SlaI CTCGAG 1 cut(s) 810
SmiMI CAYNNNNRTG 4 cut(s) 470, 484, 490, 582
SmlI CTYRAG 4 cut(s) 134, 739, 810, 946
SmoI CTYRAG 4 cut(s) 134, 739, 810, 946
SpeI ACTAGT 1 cut(s) 821
Sse9I AATT 7 cut(s) 90, 107, 234, 429, 747, 956, 968
SsiI CCGC 4 cut(s) 33, 275, 400, 951
SspMI CTAG 6 cut(s) 333, 443, 501, 647, 822, 893
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 675
TaqI TCGA 8 cut(s) 56, 164, 258, 281, 367, 426, 594, 811
TasI AATT 7 cut(s) 90, 107, 234, 429, 747, 956, 968
TfiI GAWTC 6 cut(s) 58, 130, 364, 415, 457, 554
Tru1I TTAA 2 cut(s) 816, 995
Tru9I TTAA 2 cut(s) 816, 995
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 2 cut(s) 14, 538
TseI GCWGC 6 cut(s) 86, 151, 650, 830, 875, 920
Tsp45I GTSAC 2 cut(s) 14, 538
TspDTI ATGAA 6 cut(s) 32, 120, 138, 228, 290, 818
TspGWI ACGGA 2 cut(s) 701, 766
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 774
VspI ATTAAT 1 cut(s) 995
XagI CCTNNNNNAGG 2 cut(s) 377, 465
XapI RAATTY 2 cut(s) 234, 968
XceI RCATGY 1 cut(s) 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 842
XhoI CTCGAG 1 cut(s) 810
XspI CTAG 6 cut(s) 333, 443, 501, 647, 822, 893
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.