Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002417

Physical Location & Seq

Genomic Coordinates Forward (+)

5112887 .. 5117847

Gene Structure 4961 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1072700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1851 bp

ATGCGATTGATTTGTGATGGAATGTGTTCAATGGCTCCCAAAGCGAACGCAGATGGTCGGACTAGGAGCTCGGTACAAATTTTTATTGTAGCTGGTTTGTGCTGTTTTTTCTATATATTGGGTGCTTGGCAGAGAAGTGGTTTTGGGAAGGGAGATAGTATAGCTTTAGAGATCACTAAGAATGGGGCTGACTGCAGTATCATTCCAAGTTTAACTTTTGATTCCCACCATGCTGGCGAAGCTGGGAATATTGATGAATCAAAGCCCAAAGTTTTCGAACCGTGTCAACATAGTTACACTGATTACACCCCATGCCAAGATCAAAAGCGTGCAATGACATTCCCTAGGGAAGATATGAATTATCGAGAGAGGCATTGCCCTCGTGAGGAAGAAAAGTTACATTGCCTTATTCCAGCACCAAAGGGGTATGTAACTCCATTCCCCTGGCCAAAGAGCCGTGACTATGTACCATATGCCAATGCACCATATAAGAGCTTGACTGTTGAGAAGGCTGTTCAGAACTGGATCCAATATGAGGGTAATGTATTTCGATTCCCTGGTGGGGGAACGCAGTTTCCTCAAGGGGCAGATAAATATATTGATCAACTTGCTGCTGTAATACCAATAAAGAATGGGACAGTGAGAACTGCACTGGACACTGGTTGTGGGGTTGCCAGTTGGGGTGCATACTTGTTGAGTAGACATGTTCTGGCCATGTCGTTTGCGCCAAGAGATTCCCATGAGGCACAAGTCCAATTTGCTCTTGAGAGGGGTGTACCTGCAGTTATTGGTGTTCTGGGTACAATAAGGCTGCCTTATCCATCCAGAGCCTTTGACATGGCTCATTGTTCTCGTTGCTTAATTCCATGGGGAATAAATGATGGAAAATATCTCAAGGAAGTTGACCGAGTTCTTAGGCCTGGGGGTTATTGGGTGCTTTCGGGTCCTCCAATTAATTGGAAGAATAATTACATTTCATGGCAGCGTCCCAAGGAGGATCTTGAGGAGGAACAGAGACAGATTGAAGAAGCTGCTAAACTTCTTTGCTGGGAGAAAAAGTCTGAGAAGGGTGAAACTGCCATTTGGCAAAAGAGGGTGGATTCTGATTCATGTGGAGACAGACAAGATGCTTCCCGTGCTAACTTTTGCAAAGCTGATGAAGCAGATAGCGTCTGGTATAAGAAAATGGAGGGATGCATAACACCATATCCTGAAGTTTCCAGTGGGGAGTTGAAGCCATTTCCAAAAAGGCTTTATGCTGTTCCCCCCAGGATTTCTAGTGGGTCTGTTCCCGGAGTTTCTGCTGAGGATTATGAGGAGGATAACAAAAAATGGAAGAAGCATGTAAATGCTTATAGGAGAATCAATAAACTAATTGACACAGGAAGGTATCGCAACATTATGGATATGAATGCTGGTTTGGGTGGTTTTGCTGCAGCAATTGAATCTCCAAAATTGTGGGTCATGAATGTGGTGCCCACAATAGCTGAGAAAAATACGCTGGGTGTTGTATATGAGCGAGGATTGATTGGCATCTACCATGACTGGTGCGAAGGTTTCTCTACTTACCCAAGGACATATGACCTCATTCACGCCCATGGTGTTTTCAGTTTGTACAATGGAAAATGCAATTGGGAAGACATTCTTCTTGAGATGGACAGGATTTTGCGGCCAGAAGGTGCGGTCATATTCCGCGATGAAGTTGATGTATTAATTAAGGTGAAGAAAATAGTTGGAGGAATGAGATGGGATACTAAAATGGTGGACCATGAGGATGGTCCCCTTGTACCAGAGAAGGTATTGGTTGTTGTCAAGCAGTACTGGGTTGGAAACTCCACCGACACACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

617

Amino Acids

69.57

Weight (kDa)

7.15

Isoelectric Point (pI)

36.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	101 - 605	4.4e-228	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	218 - 312	8.4e-07	Methyltransferase domain
Methyltransf_25	PF13649	218 - 309	1e-05	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002417)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26850 AT1G26850 AT1G26850
fragaria_vesca	FvH4_4g31440 FvH4_4g31440 FvH4_4g31440 FvH4_5g15080
malus_domestica	MD06G1192300.v1.1 MD13G1072700.v1.1 MD14G1198900.v1.1 MD16G1073500.v1.1
prunus_persica	Prupe.1G273400_v2.0.a1 Prupe.1G273400_v2.0.a1 Prupe.1G273400_v2.0.a1 Prupe.5G192000_v2.0.a1 Prupe.5G192000_v2.0.a1
pyrus_communis	pycom06g17040 pycom13g06520 pycom14g16510 pycom16g06360
rosa_chinensis	RchiOBHm_Chr4g0439921 RchiOBHm_Chr7g0181131
rosa_laevigata	RLG00000005211 RLG00000006225
rosa_multiflora	Rmu_sc0001147.1_g000019 Rmu_sc0022459.1_g000002
rosa_roxburghii	Rroxscaffold_3G00272250 Rroxscaffold_5G00380870
rosa_rugosa	Rorug04G0322400 Rorug04G0322500 Rorug06G0438700
rosa_samantha	Rh4AG373400 Rh4BG385700 Rh4CG400200 Rh4DG380000 Rh7AG040600 Rh7BG040000 Rh7CG042000 Rh7DG040400
rosa_wichuraiana	Rw4G031930 Rw7G003350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	787
AccB1I	GGYRCC	1 cut(s)	1474
AccI	GTMKAC	1 cut(s)	700
AccII	CGCG	1 cut(s)	1695
AciI	CCGC	3 cut(s)	1669, 1682, 1693
AclWI	GGATC	3 cut(s)	520, 533, 1005
AcoI	YGGCCR	3 cut(s)	446, 711, 1670
AcsI	RAATTY	1 cut(s)	78
AcuI	CTGAAG	1 cut(s)	1233
AfaI	GTAC	7 cut(s)	75, 468, 777, 802, 1616, 1788, 1820
AfiI	CCNNNNNNNGG	4 cut(s)	385, 535, 562, 563
AflIII	ACRYGT	1 cut(s)	703
AgsI	TTSAA	4 cut(s)	30, 1025, 1234, 1445
AjnI	CCWGG	4 cut(s)	443, 556, 919, 1268
AjuI	GAANNNNNNNTTGG	4 cut(s)	1403, 1435, 1615, 1647
AloI	GAACNNNNNNTCC	2 cut(s)	559, 591
AluBI	AGCT	8 cut(s)	69, 92, 164, 242, 495, 1031, 1154, 1487
AluI	AGCT	8 cut(s)	69, 92, 164, 242, 495, 1031, 1154, 1487
Alw21I	GWGCWC	1 cut(s)	71
Alw26I	GTCTC	2 cut(s)	1009, 1110
AlwI	GGATC	3 cut(s)	520, 533, 1005
AoxI	GGCC	4 cut(s)	446, 711, 917, 1670
ApeKI	GCWGC	6 cut(s)	611, 811, 982, 1031, 1433, 1436
ApoI	RAATTY	1 cut(s)	78
AseI	ATTAAT	2 cut(s)	954, 1712
Asp700I	GAANNNNTTC	2 cut(s)	25, 1641
AspA2I	CCTAGG	1 cut(s)	344
AspLEI	GCGC	1 cut(s)	727
AspS9I	GGNCC	3 cut(s)	944, 1765, 1778
AsuC2I	CCSGG	1 cut(s)	1293
AsuHPI	GGTGA	2 cut(s)	1082, 1732
AsuII	TTCGAA	1 cut(s)	276
AvaII	GGWCC	3 cut(s)	944, 1765, 1778
AvrII	CCTAGG	1 cut(s)	344
BaeGI	GKGCMC	1 cut(s)	1479
BaeI	ACNNNNGTAYC	2 cut(s)	181, 214
BalI	TGGCCA	2 cut(s)	448, 713
BamHI	GGATCC	1 cut(s)	525
BanI	GGYRCC	1 cut(s)	1474
BanII	GRGCYC	1 cut(s)	71
BarI	GAAGNNNNNNTAC	4 cut(s)	381, 413, 953, 985
BauI	CACGAG	1 cut(s)	381
BbsI	GAAGAC	1 cut(s)	1644
Bbv12I	GWGCWC	1 cut(s)	71
BbvCI	CCTCAGC	1 cut(s)	1305
BbvI	GCAGC	6 cut(s)	598, 798, 994, 1018, 1420, 1448
BccI	CCATC	7 cut(s)	11, 47, 829, 875, 1648, 1740, 1769
BceAI	ACGGC	1 cut(s)	441
BcgI	CGANNNNNNTGC	2 cut(s)	362, 396
BciT130I	CCWGG	4 cut(s)	445, 558, 921, 1270
BciVI	GTATCC	1 cut(s)	1744
BclI	TGATCA	1 cut(s)	601
BcnI	CCSGG	1 cut(s)	1293
BcoDI	GTCTC	2 cut(s)	1009, 1110
BfaI	CTAG	3 cut(s)	63, 345, 1278
BfmI	CTRYAG	3 cut(s)	193, 780, 1434
BfuAI	ACCTGC	1 cut(s)	787
BfuI	GTATCC	1 cut(s)	1744
BisI	GCNGC	7 cut(s)	612, 812, 983, 1032, 1434, 1437, 1670
BlnI	CCTAGG	1 cut(s)	344
BlsI	GCNGC	7 cut(s)	613, 813, 984, 1033, 1435, 1438, 1671
BmcAI	AGTACT	1 cut(s)	1820
Bme1390I	CCNGG	5 cut(s)	445, 558, 921, 1270, 1293
Bme18I	GGWCC	3 cut(s)	944, 1765, 1778
BmgT120I	GGNCC	3 cut(s)	944, 1765, 1778
BmiI	GGNNCC	5 cut(s)	36, 527, 945, 1476, 1780
BmrFI	CCNGG	5 cut(s)	445, 558, 921, 1270, 1293
BmrI	ACTGGG	1 cut(s)	1831
BmsI	GCATC	3 cut(s)	1117, 1184, 1542
BmuI	ACTGGG	1 cut(s)	1831
BpiI	GAAGAC	1 cut(s)	1644
Bpu10I	CCTNAGC	1 cut(s)	1305
Bpu14I	TTCGAA	1 cut(s)	276
BpuEI	CTTGAG	5 cut(s)	564, 785, 878, 1022, 1670
BpuMI	CCSGG	1 cut(s)	1293
BsaBI	GATNNNNATC	1 cut(s)	1532
BsaJI	CCNNGG	9 cut(s)	344, 443, 556, 866, 920, 990, 1268, 1571, 1597
BsaXI	ACNNNNNCTCC	2 cut(s)	1043, 1073
Bsc4I	CCNNNNNNNGG	4 cut(s)	385, 535, 562, 563
Bse1I	ACTGG	7 cut(s)	527, 657, 664, 675, 1221, 1550, 1826
Bse3DI	GCAATG	3 cut(s)	339, 373, 400
Bse8I	GATNNNNATC	1 cut(s)	1532
BseBI	CCWGG	4 cut(s)	445, 558, 921, 1270
BseDI	CCNNGG	9 cut(s)	344, 443, 556, 866, 920, 990, 1268, 1571, 1597
BseGI	GGATG	3 cut(s)	821, 1199, 1780
BseJI	GATNNNNATC	1 cut(s)	1532
BseLI	CCNNNNNNNGG	4 cut(s)	385, 535, 562, 563
BseMI	GCAATG	3 cut(s)	339, 373, 400
BseMII	CTCAG	3 cut(s)	1053, 1296, 1479
BseNI	ACTGG	7 cut(s)	527, 657, 664, 675, 1221, 1550, 1826
BseRI	GAGGAG	2 cut(s)	1019, 1331
BseSI	GKGCMC	1 cut(s)	1479
BseXI	GCAGC	6 cut(s)	598, 798, 994, 1018, 1420, 1448
BseYI	CCCAGC	3 cut(s)	242, 1047, 1501
BsgI	GTGCAG	1 cut(s)	633
Bsh1236I	CGCG	1 cut(s)	1695
BshFI	GGCC	4 cut(s)	448, 713, 919, 1672
BshNI	GGYRCC	1 cut(s)	1474
BsiHKAI	GWGCWC	1 cut(s)	71
BsiSI	CCGG	1 cut(s)	1293
BslFI	GGGAC	3 cut(s)	649, 972, 1764
BslI	CCNNNNNNNGG	4 cut(s)	385, 535, 562, 563
BsmAI	GTCTC	2 cut(s)	1009, 1110
BsmFI	GGGAC	3 cut(s)	649, 972, 1764
BsmI	GAATGC	1 cut(s)	1417
BsnI	GGCC	4 cut(s)	448, 713, 919, 1672
Bsp119I	TTCGAA	1 cut(s)	276
Bsp1286I	GDGCHC	2 cut(s)	71, 1479
Bsp1407I	TGTACA	1 cut(s)	1614
Bsp143I	GATC	5 cut(s)	171, 319, 525, 601, 997
Bsp19I	CCATGG	2 cut(s)	866, 1597
BspACI	CCGC	3 cut(s)	1669, 1682, 1693
BspANI	GGCC	4 cut(s)	448, 713, 919, 1672
BspCNI	CTCAG	3 cut(s)	1054, 1297, 1480
BspFNI	CGCG	1 cut(s)	1695
BspHI	TCATGA	1 cut(s)	1464
BspLI	GGNNCC	5 cut(s)	36, 527, 945, 1476, 1780
BspMAI	CTGCAG	3 cut(s)	197, 784, 1438
BspMI	ACCTGC	1 cut(s)	787
BspPI	GGATC	3 cut(s)	520, 533, 1005
BspT104I	TTCGAA	1 cut(s)	276
BspT107I	GGYRCC	1 cut(s)	1474
BsrDI	GCAATG	3 cut(s)	339, 373, 400
BsrGI	TGTACA	1 cut(s)	1614
BsrI	ACTGG	7 cut(s)	527, 657, 664, 675, 1221, 1550, 1826
BssECI	CCNNGG	9 cut(s)	344, 443, 556, 866, 920, 990, 1268, 1571, 1597
BssMI	GATC	5 cut(s)	171, 319, 525, 601, 997
BssSI	CACGAG	1 cut(s)	381
BssT1I	CCWWGG	5 cut(s)	344, 866, 990, 1571, 1597
Bst2BI	CACGAG	1 cut(s)	381
Bst2UI	CCWGG	4 cut(s)	445, 558, 921, 1270
Bst4CI	ACNGT	3 cut(s)	282, 502, 640
BstAUI	TGTACA	1 cut(s)	1614
BstBI	TTCGAA	1 cut(s)	276
BstC8I	GCNNGC	2 cut(s)	235, 330
BstDEI	CTNAG	5 cut(s)	177, 914, 1062, 1305, 1488
BstDSI	CCRYGG	2 cut(s)	866, 1597
BstF5I	GGATG	3 cut(s)	821, 1199, 1780
BstFNI	CGCG	1 cut(s)	1695
BstHHI	GCGC	1 cut(s)	727
BstKTI	GATC	5 cut(s)	174, 322, 528, 604, 1000
BstMAI	GTCTC	2 cut(s)	1009, 1110
BstMBI	GATC	5 cut(s)	171, 319, 525, 601, 997
BstMWI	GCNNNNNNNGC	4 cut(s)	41, 239, 1136, 1160
BstNI	CCWGG	4 cut(s)	445, 558, 921, 1270
BstNSI	RCATGY	2 cut(s)	707, 1346
BstSCI	CCNGG	5 cut(s)	443, 556, 919, 1268, 1291
BstSFI	CTRYAG	3 cut(s)	193, 780, 1434
BstSLI	GKGCMC	1 cut(s)	1479
BstUI	CGCG	1 cut(s)	1695
BstV1I	GCAGC	6 cut(s)	598, 798, 994, 1018, 1420, 1448
BstV2I	GAAGAC	1 cut(s)	1644
BstX2I	RGATCY	2 cut(s)	525, 997
BstXI	CCANNNNNNTGG	5 cut(s)	233, 444, 957, 1458, 1775
BstYI	RGATCY	2 cut(s)	525, 997
BsuI	GTATCC	1 cut(s)	1744
BsuRI	GGCC	4 cut(s)	448, 713, 919, 1672
BtgI	CCRYGG	2 cut(s)	866, 1597
BtgZI	GCGATG	1 cut(s)	1710
BtsCI	GGATG	3 cut(s)	821, 1199, 1780
BtsIMutI	CAGTG	5 cut(s)	297, 645, 650, 657, 1228
BveI	ACCTGC	1 cut(s)	787
Cac8I	GCNNGC	2 cut(s)	235, 330
CciI	TCATGA	1 cut(s)	1464
CfoI	GCGC	1 cut(s)	727
Cfr13I	GGNCC	3 cut(s)	944, 1765, 1778
CseI	GACGC	2 cut(s)	974, 1159
Csp6I	GTAC	7 cut(s)	74, 467, 776, 801, 1615, 1787, 1819
CviQI	GTAC	7 cut(s)	74, 467, 776, 801, 1615, 1787, 1819
DdeI	CTNAG	5 cut(s)	177, 914, 1062, 1305, 1488
DpnI	GATC	5 cut(s)	173, 321, 527, 603, 999
DpnII	GATC	5 cut(s)	171, 319, 525, 601, 997
EaeI	YGGCCR	3 cut(s)	446, 711, 1670
Ecl136II	GAGCTC	1 cut(s)	69
Eco130I	CCWWGG	5 cut(s)	344, 866, 990, 1571, 1597
Eco147I	AGGCCT	1 cut(s)	919
Eco24I	GRGCYC	1 cut(s)	71
Eco47I	GGWCC	3 cut(s)	944, 1765, 1778
Eco53kI	GAGCTC	1 cut(s)	69
Eco57I	CTGAAG	1 cut(s)	1233
EcoICRI	GAGCTC	1 cut(s)	69
EcoO109I	RGGNCCY	1 cut(s)	944
EcoRII	CCWGG	4 cut(s)	443, 556, 919, 1268
EcoT14I	CCWWGG	5 cut(s)	344, 866, 990, 1571, 1597
EcoT22I	ATGCAT	1 cut(s)	1199
EcoT38I	GRGCYC	1 cut(s)	71
ErhI	CCWWGG	5 cut(s)	344, 866, 990, 1571, 1597
FalI	AAGNNNNNCTT	6 cut(s)	199, 231, 799, 831, 1629, 1661
FaqI	GGGAC	3 cut(s)	649, 972, 1764
FauNDI	CATATG	2 cut(s)	472, 1579
FbaI	TGATCA	1 cut(s)	601
FblI	GTMKAC	1 cut(s)	700
Fnu4HI	GCNGC	7 cut(s)	612, 812, 983, 1032, 1434, 1437, 1670
FokI	GGATG	3 cut(s)	808, 1206, 1787
FriOI	GRGCYC	1 cut(s)	71
Fsp4HI	GCNGC	7 cut(s)	612, 812, 983, 1032, 1434, 1437, 1670
FspBI	CTAG	3 cut(s)	63, 345, 1278
GlaI	GCGC	1 cut(s)	726
GluI	GCNGC	7 cut(s)	612, 812, 983, 1032, 1434, 1437, 1670
GsaI	CCCAGC	3 cut(s)	246, 1051, 1505
HaeIII	GGCC	4 cut(s)	448, 713, 919, 1672
HapII	CCGG	1 cut(s)	1293
HgaI	GACGC	2 cut(s)	974, 1159
HhaI	GCGC	1 cut(s)	727
Hin6I	GCGC	1 cut(s)	725
HinP1I	GCGC	1 cut(s)	725
HincII	GTYRAC	2 cut(s)	287, 904
HindII	GTYRAC	2 cut(s)	287, 904
HinfI	GANTC	8 cut(s)	221, 257, 552, 734, 1100, 1106, 1362, 1445
HpaII	CCGG	1 cut(s)	1293
HphI	GGTGA	2 cut(s)	1082, 1732
Hpy166II	GTNNAC	5 cut(s)	287, 701, 776, 904, 1765
Hpy188I	TCNGA	4 cut(s)	60, 519, 1063, 1105
Hpy188III	TCNNGA	8 cut(s)	365, 383, 764, 825, 1001, 1211, 1465, 1649
Hpy8I	GTNNAC	5 cut(s)	287, 701, 776, 904, 1765
HpyAV	CCTTC	7 cut(s)	142, 502, 1060, 1380, 1547, 1670, 1789
HpyCH4III	ACNGT	3 cut(s)	282, 502, 640
HpyF10VI	GCNNNNNNNGC	4 cut(s)	41, 239, 1136, 1160
HpyF3I	CTNAG	5 cut(s)	177, 914, 1062, 1305, 1488
HspAI	GCGC	1 cut(s)	725
Ksp22I	TGATCA	1 cut(s)	601
Kzo9I	GATC	5 cut(s)	171, 319, 525, 601, 997
LmnI	GCTCC	2 cut(s)	40, 66
Lsp1109I	GCAGC	6 cut(s)	598, 798, 994, 1018, 1420, 1448
LweI	GCATC	3 cut(s)	1117, 1184, 1542
MaeI	CTAG	3 cut(s)	63, 345, 1278
MaeIII	GTNAC	4 cut(s)	293, 396, 430, 458
MalI	GATC	5 cut(s)	173, 321, 527, 603, 999
MboI	GATC	5 cut(s)	171, 319, 525, 601, 997
MboII	GAAGA	8 cut(s)	362, 401, 973, 1037, 1348, 1637, 1649, 1735
MfeI	CAATTG	2 cut(s)	1440, 1630
MflI	RGATCY	2 cut(s)	525, 997
MhlI	GDGCHC	2 cut(s)	71, 1479
MlsI	TGGCCA	2 cut(s)	448, 713
MluNI	TGGCCA	2 cut(s)	448, 713
MmeI	TCCRAC	3 cut(s)	38, 1714, 1807
Mox20I	TGGCCA	2 cut(s)	448, 713
Mph1103I	ATGCAT	1 cut(s)	1199
MroXI	GAANNNNTTC	2 cut(s)	25, 1641
MscI	TGGCCA	2 cut(s)	448, 713
MseI	TTAA	5 cut(s)	212, 860, 954, 1712, 1716
MslI	CAYNNNNRTG	4 cut(s)	1347, 1469, 1596, 1773
Msp20I	TGGCCA	2 cut(s)	448, 713
MspI	CCGG	1 cut(s)	1293
MspR9I	CCNGG	5 cut(s)	445, 558, 921, 1270, 1293
MunI	CAATTG	2 cut(s)	1440, 1630
Mva1269I	GAATGC	1 cut(s)	1417
MvaI	CCWGG	4 cut(s)	445, 558, 921, 1270
MvnI	CGCG	1 cut(s)	1695
MwoI	GCNNNNNNNGC	4 cut(s)	41, 239, 1136, 1160
NciI	CCSGG	1 cut(s)	1293
NcoI	CCATGG	2 cut(s)	866, 1597
NdeI	CATATG	2 cut(s)	472, 1579
NdeII	GATC	5 cut(s)	171, 319, 525, 601, 997
NlaIV	GGNNCC	5 cut(s)	36, 527, 945, 1476, 1780
NmuCI	GTSAC	1 cut(s)	458
NsiI	ATGCAT	1 cut(s)	1199
NspI	RCATGY	2 cut(s)	707, 1346
NspV	TTCGAA	1 cut(s)	276
PacI	TTAATTAA	1 cut(s)	1716
PagI	TCATGA	1 cut(s)	1464
PceI	AGGCCT	1 cut(s)	919
PciI	ACATGT	1 cut(s)	703
PctI	GAATGC	1 cut(s)	1417
PdmI	GAANNNNTTC	2 cut(s)	25, 1641
PfeI	GAWTC	8 cut(s)	221, 257, 552, 734, 1100, 1106, 1362, 1445
PfoI	TCCNGGA	1 cut(s)	1291
PkrI	GCNGC	7 cut(s)	613, 813, 984, 1033, 1435, 1438, 1671
PpuMI	RGGWCCY	1 cut(s)	944
PscI	ACATGT	1 cut(s)	703
PshBI	ATTAAT	2 cut(s)	954, 1712
Psp124BI	GAGCTC	1 cut(s)	71
Psp5II	RGGWCCY	1 cut(s)	944
Psp6I	CCWGG	4 cut(s)	443, 556, 919, 1268
PspFI	CCCAGC	3 cut(s)	242, 1047, 1501
PspGI	CCWGG	4 cut(s)	443, 556, 919, 1268
PspN4I	GGNNCC	5 cut(s)	36, 527, 945, 1476, 1780
PspPI	GGNCC	3 cut(s)	944, 1765, 1778
PspPPI	RGGWCCY	1 cut(s)	944
PstI	CTGCAG	3 cut(s)	197, 784, 1438
PsuI	RGATCY	2 cut(s)	525, 997
RsaI	GTAC	7 cut(s)	75, 468, 777, 802, 1616, 1788, 1820
RsaNI	GTAC	7 cut(s)	74, 467, 776, 801, 1615, 1787, 1819
RseI	CAYNNNNRTG	4 cut(s)	1347, 1469, 1596, 1773
SacI	GAGCTC	1 cut(s)	71
SaqAI	TTAA	5 cut(s)	212, 860, 954, 1712, 1716
SatI	GCNGC	7 cut(s)	612, 812, 983, 1032, 1434, 1437, 1670
Sau3AI	GATC	5 cut(s)	171, 319, 525, 601, 997
Sau96I	GGNCC	3 cut(s)	944, 1765, 1778
ScaI	AGTACT	1 cut(s)	1820
ScrFI	CCNGG	5 cut(s)	445, 558, 921, 1270, 1293
SduI	GDGCHC	2 cut(s)	71, 1479
SfaNI	GCATC	3 cut(s)	1117, 1184, 1542
SfcI	CTRYAG	3 cut(s)	193, 780, 1434
SfuI	TTCGAA	1 cut(s)	276
SinI	GGWCC	3 cut(s)	944, 1765, 1778
SmiMI	CAYNNNNRTG	4 cut(s)	1347, 1469, 1596, 1773
SmlI	CTYRAG	5 cut(s)	579, 764, 893, 1001, 1649
SmoI	CTYRAG	5 cut(s)	579, 764, 893, 1001, 1649
SseBI	AGGCCT	1 cut(s)	919
SsiI	CCGC	3 cut(s)	1669, 1682, 1693
SspI	AATATT	1 cut(s)	250
SspMI	CTAG	3 cut(s)	63, 345, 1278
SstI	GAGCTC	1 cut(s)	71
StuI	AGGCCT	1 cut(s)	919
StyD4I	CCNGG	5 cut(s)	443, 556, 919, 1268, 1291
StyI	CCWWGG	5 cut(s)	344, 866, 990, 1571, 1597
TaaI	ACNGT	3 cut(s)	282, 502, 640
TaqI	TCGA	3 cut(s)	276, 364, 550
TaqII	GACCGA	1 cut(s)	921
TatI	WGTACW	2 cut(s)	1614, 1818
TauI	GCSGC	1 cut(s)	1672
TfiI	GAWTC	8 cut(s)	221, 257, 552, 734, 1100, 1106, 1362, 1445
Tru1I	TTAA	5 cut(s)	212, 860, 954, 1712, 1716
Tru9I	TTAA	5 cut(s)	212, 860, 954, 1712, 1716
TscAI	CASTG	5 cut(s)	304, 645, 657, 664, 1228
TseFI	GTSAC	1 cut(s)	458
TseI	GCWGC	6 cut(s)	611, 811, 982, 1031, 1433, 1436
Tsp45I	GTSAC	1 cut(s)	458
TspDTI	ATGAA	8 cut(s)	270, 371, 966, 1098, 1173, 1424, 1481, 1713
TspRI	CASTG	5 cut(s)	304, 645, 657, 664, 1228
VpaK11BI	GGWCC	3 cut(s)	944, 1765, 1778
VspI	ATTAAT	2 cut(s)	954, 1712
XapI	RAATTY	1 cut(s)	78
XceI	RCATGY	2 cut(s)	707, 1346
XmaJI	CCTAGG	1 cut(s)	344
XmiI	GTMKAC	1 cut(s)	700
XmnI	GAANNNNTTC	2 cut(s)	25, 1641
XspI	CTAG	3 cut(s)	63, 345, 1278
ZrmI	AGTACT	1 cut(s)	1820
Zsp2I	ATGCAT	1 cut(s)	1199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.