Rw4G031930

Methyltransferase

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
Physical Location & Seq
Forward (+)
59122152 .. 59125359
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031930.1

Sequence Viewer

Length: 1512 bp
ATGACATTTCCCAGAGAGGATATGAATTATCGAGAGAGGCATTGCCCTCCCGAGGAAGAAAAGTTGCATTGCCTCATTCCGGCACCAAAGGGATATGTAACTCCGTTCCCCTGGCCAAAGAGCCGTGACTATGTGCCGTATGCAAATGCACCGTATAAGAGCTTGACTGTTGAGAAGGCTGTCCAGAACTGGATCCAGTATGAGGGTAATGTATTTAGATTCCCTGGCGGGGGAACACAGTTTCCTCAAGGGGCAGATAAATATATTGATCAGCTTGCTTCGGTGATACCAATTAAGAATGGCATGGTTAGAACTGCGCTGGACACTGGTTGTGGGGTTGCTAGTTGGGGTGCGTATTTGTTGAGTAGAGATGTTATTGCCATGTCATTTGCACCAAGAGATTCACATGAAGCACAGGTACAATTTGCTCTTGAAAGGGGTGTACCTGCAGTAATTGGTGTTCTGGGGACAATAAAGCTGCCATATCCATCCAGAGCCTTTGACATGGCTCACTGTTCACGTTGCTTAATTCCATGGGGAGTAAATGATGGAAAATATCTCATGGAAGTTGACCGAGTCCTTAGGCCTGGGGGATATTGGGTTCTTTCGGGTCCCCCCATCAATTGGAAGAATAACTACAAATCATGGCAGCGTCCCAAGGAGGAACTTGAGGAGGAACAGAGAAAGATTGAAGAAGTTGCTAAACTTCTATGCTGGGAGAAGAAGTCTGAGAAGGGTGAAACCGCCATTTGGCAGAAGAGGGTGAATTCTGATTCATGCAGCAGCAGACAAGATGACTCCCGAGCTACCTTTTGCAACTCTAATGAGCAGATGATGTATAAGAAAATGGAGGGATGTATAACTCCATATCCTGACGAAGTTGGTGGAGAACTGAAGCCATTTCCTGAAAGGCTTCATGCTGTACCACCTAGAATTGCTAGTGGATCGGTTCCTGGAGTTTCTGTTGAGTCCTACCTGGAGGATAACAACAAATGGAAGAAGCATGTTAGTGCTTATAAAAGAATCAACAAACTAATTGACTCTGGAAGGTATCGCAACATAATGGATATGAATGCTGGTTTGGGTGGTTTTGCTGCAGCACTTACATCTCCAAAATTGTGGGTCATGAATGTGTTGCCCACTATAGCTGAGAAAAATACATTAGGTGCTGTATATGAGCGAGGATTGATCGGAATCTATCACGACTGGTGCGAAGGTTTCTCCACATACCCAAGGACATATGATCTCATTCATGCCTATGGTGTTTTCAGTTTGTACAATGGCAAATGCAATTGGGAAGACATTCTTCTAGAGATGGACCGAATTTTGAGGCCTGAAGGTGCTGTCATATTCCGGGATGAAGTTGATGTATTAATTAAGGTGAAGAAGATAGTTGGAGGGATGAGGTGGGACACCAAAATGGTGGACCATGAAGATGGTCCCCTTGTTCCGGAGAAGGTATTGGTTGCCGTCAAGCAGTACTGGGTTGGAAACTCCACATCCACACAATGA

Protein Analysis

503

Amino Acids

57.12

Weight (kDa)

7.89

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 4 - 492 1e-219 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 107 - 201 5.2e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 454
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 624
AccIII TCCGGA 1 cut(s) 1450
AciI CCGC 2 cut(s) 228, 744
AclWI GGATC 3 cut(s) 187, 200, 952
AcoI YGGCCR 1 cut(s) 113
AcsI RAATTY 2 cut(s) 766, 1323
AcuI CTGAAG 2 cut(s) 914, 1356
AfaI GTAC 5 cut(s) 420, 444, 924, 1277, 1481
AfiI CCNNNNNNNGG 8 cut(s) 52, 79, 202, 229, 230, 624, 750, 1450
AgsI TTSAA 2 cut(s) 434, 692
AjnI CCWGG 5 cut(s) 110, 223, 586, 952, 975
AjuI GAANNNNNNNTTGG 2 cut(s) 1064, 1096
AloI GAACNNNNNNTCC 4 cut(s) 226, 258, 585, 617
AluBI AGCT 5 cut(s) 162, 274, 478, 806, 1148
AluI AGCT 5 cut(s) 162, 274, 478, 806, 1148
AlwI GGATC 3 cut(s) 187, 200, 952
Ama87I CYCGRG 2 cut(s) 50, 801
Aor13HI TCCGGA 1 cut(s) 1450
AoxI GGCC 3 cut(s) 113, 584, 1331
ApeKI GCWGC 6 cut(s) 478, 649, 780, 783, 1094, 1097
ApoI RAATTY 2 cut(s) 766, 1323
AseI ATTAAT 1 cut(s) 1373
Asp700I GAANNNNTTC 2 cut(s) 912, 1302
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 4 cut(s) 611, 1318, 1426, 1439
AsuC2I CCSGG 1 cut(s) 1355
AsuHPI GGTGA 4 cut(s) 295, 749, 775, 1393
AvaI CYCGRG 2 cut(s) 50, 801
AvaII GGWCC 4 cut(s) 611, 1318, 1426, 1439
AxyI CCTNAGG 1 cut(s) 581
BalI TGGCCA 1 cut(s) 115
BamHI GGATCC 1 cut(s) 192
BanI GGYRCC 1 cut(s) 82
BarI GAAGNNNNNNTAC 4 cut(s) 402, 434, 620, 652
BbsI GAAGAC 1 cut(s) 1305
BbvI GCAGC 6 cut(s) 465, 661, 792, 795, 1081, 1109
BccI CCATC 5 cut(s) 496, 542, 626, 1309, 1430
BceAI ACGGC 3 cut(s) 108, 121, 1454
BciT130I CCWGG 5 cut(s) 112, 225, 588, 954, 977
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 1 cut(s) 1355
BfaI CTAG 4 cut(s) 342, 930, 939, 1310
BfmI CTRYAG 3 cut(s) 447, 1095, 1143
BfuAI ACCTGC 1 cut(s) 454
BisI GCNGC 6 cut(s) 479, 650, 781, 784, 1095, 1098
BlsI GCNGC 6 cut(s) 480, 651, 782, 785, 1096, 1099
BmcAI AGTACT 1 cut(s) 1481
Bme1390I CCNGG 6 cut(s) 112, 225, 588, 954, 977, 1355
Bme18I GGWCC 4 cut(s) 611, 1318, 1426, 1439
BmeT110I CYCGRG 2 cut(s) 50, 801
BmgT120I GGNCC 4 cut(s) 611, 1318, 1426, 1439
BmiI GGNNCC 6 cut(s) 84, 194, 612, 613, 951, 1441
BmrFI CCNGG 6 cut(s) 112, 225, 588, 954, 977, 1355
BmrI ACTGGG 1 cut(s) 1492
BmuI ACTGGG 1 cut(s) 1492
BpiI GAAGAC 1 cut(s) 1305
BpmI CTGGAG 2 cut(s) 975, 998
BpuEI CTTGAG 2 cut(s) 231, 689
BpuMI CCSGG 1 cut(s) 1355
BsaBI GATNNNNATC 1 cut(s) 1193
BsaJI CCNNGG 7 cut(s) 51, 110, 223, 533, 587, 657, 1232
BsaWI WCCGGW 1 cut(s) 1450
BsaXI ACNNNNNCTCC 4 cut(s) 710, 740, 948, 978
Bsc4I CCNNNNNNNGG 8 cut(s) 52, 79, 202, 229, 230, 624, 750, 1450
Bse1I ACTGG 5 cut(s) 194, 196, 331, 1211, 1487
Bse21I CCTNAGG 1 cut(s) 581
Bse3DI GCAATG 2 cut(s) 40, 67
Bse8I GATNNNNATC 1 cut(s) 1193
BseAI TCCGGA 1 cut(s) 1450
BseBI CCWGG 5 cut(s) 112, 225, 588, 954, 977
BseDI CCNNGG 7 cut(s) 51, 110, 223, 533, 587, 657, 1232
BseGI GGATG 5 cut(s) 488, 860, 1363, 1407, 1499
BseJI GATNNNNATC 1 cut(s) 1193
BseLI CCNNNNNNNGG 8 cut(s) 52, 79, 202, 229, 230, 624, 750, 1450
BseMI GCAATG 2 cut(s) 40, 67
BseMII CTCAG 2 cut(s) 720, 1140
BseNI ACTGG 5 cut(s) 194, 196, 331, 1211, 1487
BseRI GAGGAG 1 cut(s) 686
BseXI GCAGC 6 cut(s) 465, 661, 792, 795, 1081, 1109
BseYI CCCAGC 1 cut(s) 714
BshFI GGCC 3 cut(s) 115, 586, 1333
BshNI GGYRCC 1 cut(s) 82
BsiHKCI CYCGRG 2 cut(s) 50, 801
BsiSI CCGG 3 cut(s) 80, 1354, 1451
BslFI GGGAC 5 cut(s) 481, 597, 639, 1424, 1425
BslI CCNNNNNNNGG 8 cut(s) 52, 79, 202, 229, 230, 624, 750, 1450
BsmFI GGGAC 5 cut(s) 481, 597, 639, 1424, 1425
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 3 cut(s) 115, 586, 1333
BsoBI CYCGRG 2 cut(s) 50, 801
Bsp13I TCCGGA 1 cut(s) 1450
Bsp1407I TGTACA 1 cut(s) 1275
Bsp143I GATC 5 cut(s) 192, 268, 944, 1188, 1243
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 2 cut(s) 228, 744
BspANI GGCC 3 cut(s) 115, 586, 1333
BspCNI CTCAG 2 cut(s) 721, 1141
BspEI TCCGGA 1 cut(s) 1450
BspHI TCATGA 1 cut(s) 1125
BspLI GGNNCC 6 cut(s) 84, 194, 612, 613, 951, 1441
BspMAI CTGCAG 2 cut(s) 451, 1099
BspMI ACCTGC 1 cut(s) 454
BspPI GGATC 3 cut(s) 187, 200, 952
BspT107I GGYRCC 1 cut(s) 82
BsrDI GCAATG 2 cut(s) 40, 67
BsrGI TGTACA 1 cut(s) 1275
BsrI ACTGG 5 cut(s) 194, 196, 331, 1211, 1487
BssECI CCNNGG 7 cut(s) 51, 110, 223, 533, 587, 657, 1232
BssMI GATC 5 cut(s) 192, 268, 944, 1188, 1243
BssT1I CCWWGG 3 cut(s) 533, 657, 1232
Bst2UI CCWGG 5 cut(s) 112, 225, 588, 954, 977
Bst4CI ACNGT 4 cut(s) 153, 169, 240, 515
Bst6I CTCTTC 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 1275
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 3 cut(s) 581, 729, 1149
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 5 cut(s) 488, 860, 1363, 1407, 1499
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 5 cut(s) 195, 271, 947, 1191, 1246
BstMBI GATC 5 cut(s) 192, 268, 944, 1188, 1243
BstNI CCWGG 5 cut(s) 112, 225, 588, 954, 977
BstNSI RCATGY 1 cut(s) 1007
BstSCI CCNGG 6 cut(s) 110, 223, 586, 952, 975, 1353
BstSFI CTRYAG 3 cut(s) 447, 1095, 1143
BstV1I GCAGC 6 cut(s) 465, 661, 792, 795, 1081, 1109
BstV2I GAAGAC 1 cut(s) 1305
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 3 cut(s) 1119, 1423, 1436
BstYI RGATCY 1 cut(s) 192
Bsu36I CCTNAGG 1 cut(s) 581
BsuRI GGCC 3 cut(s) 115, 586, 1333
BtgI CCRYGG 1 cut(s) 533
BtsCI GGATG 5 cut(s) 488, 860, 1363, 1407, 1499
BtsIMutI CAGTG 2 cut(s) 324, 511
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 1 cut(s) 276
CciI TCATGA 1 cut(s) 1125
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 4 cut(s) 611, 1318, 1426, 1439
CseI GACGC 1 cut(s) 641
Csp6I GTAC 5 cut(s) 419, 443, 923, 1276, 1480
CviQI GTAC 5 cut(s) 419, 443, 923, 1276, 1480
DdeI CTNAG 3 cut(s) 581, 729, 1149
DpnI GATC 5 cut(s) 194, 270, 946, 1190, 1245
DpnII GATC 5 cut(s) 192, 268, 944, 1188, 1243
EaeI YGGCCR 1 cut(s) 113
Eam1104I CTCTTC 1 cut(s) 752
EarI CTCTTC 1 cut(s) 752
Eco130I CCWWGG 3 cut(s) 533, 657, 1232
Eco147I AGGCCT 2 cut(s) 586, 1333
Eco47I GGWCC 4 cut(s) 611, 1318, 1426, 1439
Eco57I CTGAAG 2 cut(s) 914, 1356
Eco81I CCTNAGG 1 cut(s) 581
Eco88I CYCGRG 2 cut(s) 50, 801
EcoO109I RGGNCCY 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 766
EcoRII CCWGG 5 cut(s) 110, 223, 586, 952, 975
EcoT14I CCWWGG 3 cut(s) 533, 657, 1232
ErhI CCWWGG 3 cut(s) 533, 657, 1232
FalI AAGNNNNNCTT 2 cut(s) 1290, 1322
FaqI GGGAC 5 cut(s) 481, 597, 639, 1424, 1425
FauI CCCGC 1 cut(s) 221
FauNDI CATATG 1 cut(s) 1240
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 6 cut(s) 479, 650, 781, 784, 1095, 1098
FokI GGATG 5 cut(s) 475, 867, 1370, 1414, 1486
Fsp4HI GCNGC 6 cut(s) 479, 650, 781, 784, 1095, 1098
FspBI CTAG 4 cut(s) 342, 930, 939, 1310
GlaI GCGC 1 cut(s) 318
GluI GCNGC 6 cut(s) 479, 650, 781, 784, 1095, 1098
GsaI CCCAGC 1 cut(s) 718
GsuI CTGGAG 2 cut(s) 975, 998
HaeIII GGCC 3 cut(s) 115, 586, 1333
HapII CCGG 3 cut(s) 80, 1354, 1451
HgaI GACGC 1 cut(s) 641
HhaI GCGC 1 cut(s) 319
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 9 cut(s) 219, 401, 576, 773, 797, 968, 1023, 1040, 1194
HpaII CCGG 3 cut(s) 80, 1354, 1451
HphI GGTGA 4 cut(s) 295, 749, 775, 1393
Hpy166II GTNNAC 4 cut(s) 443, 518, 571, 1426
Hpy188I TCNGA 3 cut(s) 730, 772, 1193
Hpy8I GTNNAC 4 cut(s) 443, 518, 571, 1426
HpyAV CCTTC 6 cut(s) 169, 727, 1041, 1208, 1331, 1450
HpyCH4III ACNGT 4 cut(s) 153, 169, 240, 515
HpyCH4IV ACGT 1 cut(s) 520
HpyCH4V TGCA 9 cut(s) 67, 143, 149, 392, 449, 780, 816, 1097, 1290
HpyF3I CTNAG 3 cut(s) 581, 729, 1149
HpySE526I ACGT 1 cut(s) 520
HspAI GCGC 1 cut(s) 317
KflI GGGWCCC 1 cut(s) 611
Kpn2I TCCGGA 1 cut(s) 1450
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 5 cut(s) 192, 268, 944, 1188, 1243
Lsp1109I GCAGC 6 cut(s) 465, 661, 792, 795, 1081, 1109
MaeI CTAG 4 cut(s) 342, 930, 939, 1310
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 2 cut(s) 97, 125
MalI GATC 5 cut(s) 194, 270, 946, 1190, 1245
MboI GATC 5 cut(s) 192, 268, 944, 1188, 1243
MfeI CAATTG 2 cut(s) 622, 1291
MflI RGATCY 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 115
MluNI TGGCCA 1 cut(s) 115
MlyI GAGTC 4 cut(s) 585, 791, 977, 1034
MmeI TCCRAC 2 cut(s) 1375, 1468
Mox20I TGGCCA 1 cut(s) 115
MroI TCCGGA 1 cut(s) 1450
MroXI GAANNNNTTC 2 cut(s) 912, 1302
MscI TGGCCA 1 cut(s) 115
MseI TTAA 4 cut(s) 294, 527, 1373, 1377
MslI CAYNNNNRTG 5 cut(s) 1008, 1130, 1257, 1418, 1434
Msp20I TGGCCA 1 cut(s) 115
MspI CCGG 3 cut(s) 80, 1354, 1451
MspR9I CCNGG 6 cut(s) 112, 225, 588, 954, 977, 1355
MunI CAATTG 2 cut(s) 622, 1291
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 5 cut(s) 112, 225, 588, 954, 977
NciI CCSGG 1 cut(s) 1355
NcoI CCATGG 1 cut(s) 533
NdeI CATATG 1 cut(s) 1240
NdeII GATC 5 cut(s) 192, 268, 944, 1188, 1243
NlaIV GGNNCC 6 cut(s) 84, 194, 612, 613, 951, 1441
NmuCI GTSAC 1 cut(s) 125
NspI RCATGY 1 cut(s) 1007
PacI TTAATTAA 1 cut(s) 1377
PagI TCATGA 1 cut(s) 1125
PceI AGGCCT 2 cut(s) 586, 1333
PcsI WCGNNNNNNNCGW 1 cut(s) 1209
PctI GAATGC 1 cut(s) 1078
PdmI GAANNNNTTC 2 cut(s) 912, 1302
PfeI GAWTC 5 cut(s) 219, 401, 773, 1023, 1194
PflFI GACNNNGTC 1 cut(s) 575
PflMI CCANNNNNTGG 1 cut(s) 624
PfoI TCCNGGA 2 cut(s) 952, 1353
PkrI GCNGC 6 cut(s) 480, 651, 782, 785, 1096, 1099
PleI GAGTC 4 cut(s) 584, 791, 976, 1034
PpsI GAGTC 4 cut(s) 584, 791, 976, 1034
PpuMI RGGWCCY 1 cut(s) 611
PshBI ATTAAT 1 cut(s) 1373
PsiI TTATAA 1 cut(s) 1017
Psp5II RGGWCCY 1 cut(s) 611
Psp6I CCWGG 5 cut(s) 110, 223, 586, 952, 975
PspFI CCCAGC 1 cut(s) 714
PspGI CCWGG 5 cut(s) 110, 223, 586, 952, 975
PspN4I GGNNCC 6 cut(s) 84, 194, 612, 613, 951, 1441
PspPI GGNCC 4 cut(s) 611, 1318, 1426, 1439
PspPPI RGGWCCY 1 cut(s) 611
PsrI GAACNNNNNNTAC 2 cut(s) 444, 476
PstI CTGCAG 2 cut(s) 451, 1099
PsuI RGATCY 1 cut(s) 192
PsyI GACNNNGTC 1 cut(s) 575
RsaI GTAC 5 cut(s) 420, 444, 924, 1277, 1481
RsaNI GTAC 5 cut(s) 419, 443, 923, 1276, 1480
RseI CAYNNNNRTG 5 cut(s) 1008, 1130, 1257, 1418, 1434
SaqAI TTAA 4 cut(s) 294, 527, 1373, 1377
SatI GCNGC 6 cut(s) 479, 650, 781, 784, 1095, 1098
Sau3AI GATC 5 cut(s) 192, 268, 944, 1188, 1243
Sau96I GGNCC 4 cut(s) 611, 1318, 1426, 1439
ScaI AGTACT 1 cut(s) 1481
SchI GAGTC 4 cut(s) 585, 791, 977, 1034
ScrFI CCNGG 6 cut(s) 112, 225, 588, 954, 977, 1355
SfcI CTRYAG 3 cut(s) 447, 1095, 1143
SinI GGWCC 4 cut(s) 611, 1318, 1426, 1439
SmiMI CAYNNNNRTG 5 cut(s) 1008, 1130, 1257, 1418, 1434
SmlI CTYRAG 2 cut(s) 246, 668
SmoI CTYRAG 2 cut(s) 246, 668
SseBI AGGCCT 2 cut(s) 586, 1333
SsiI CCGC 2 cut(s) 228, 744
SspMI CTAG 4 cut(s) 342, 930, 939, 1310
StuI AGGCCT 2 cut(s) 586, 1333
StyD4I CCNGG 6 cut(s) 110, 223, 586, 952, 975, 1353
StyI CCWWGG 3 cut(s) 533, 657, 1232
TaaI ACNGT 4 cut(s) 153, 169, 240, 515
TaiI ACGT 1 cut(s) 523
TaqI TCGA 1 cut(s) 31
TaqII GACCGA 2 cut(s) 588, 1335
TatI WGTACW 2 cut(s) 1275, 1479
TfiI GAWTC 5 cut(s) 219, 401, 773, 1023, 1194
Tru1I TTAA 4 cut(s) 294, 527, 1373, 1377
Tru9I TTAA 4 cut(s) 294, 527, 1373, 1377
TscAI CASTG 2 cut(s) 331, 518
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 6 cut(s) 478, 649, 780, 783, 1094, 1097
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 9 cut(s) 38, 423, 765, 905, 1085, 1142, 1241, 1374, 1446
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 331, 518
Tth111I GACNNNGTC 1 cut(s) 575
Van91I CCANNNNNTGG 1 cut(s) 624
VpaK11BI GGWCC 4 cut(s) 611, 1318, 1426, 1439
VspI ATTAAT 1 cut(s) 1373
XapI RAATTY 2 cut(s) 766, 1323
XbaI TCTAGA 1 cut(s) 1309
XceI RCATGY 1 cut(s) 1007
XmnI GAANNNNTTC 2 cut(s) 912, 1302
XspI CTAG 4 cut(s) 342, 930, 939, 1310
ZrmI AGTACT 1 cut(s) 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.