Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002417

Physical Location & Seq

Genomic Coordinates Forward (+)

5157528 .. 5162637

Gene Structure 5110 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1073500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGGCTCCGAAAGCGAATGCAGACGGTCGGACTAGGAGCTCGGTGCAAATTTTTATTGCAGCTGGTCTGTGCTGTTTTTTCTATATTTTGGGTGCTTGGCAGAGAAGTGGTTTTGGAAAGGGAGATAGTATCGCTTTAGCGATCACTAAGAATGAGGCTGACTGCAATATTATTCCAAGTCTAAGTTTTGATTCCCAACATGCCGGTGAAGTTGGAAATATCGATGAATCTGAATCGAAGCCCAAAGTTTTCGAACCGTGTCATCATCGTTACACTGATTACACCCCGTGCCAAGATCAGAAGCGTGCAATGACATTCCCAAGGGAAGATATGAATTATCGAGAGAGGCATTGCCCTCCTGAGGAAGAAAAGTTACATTGCCTTATTCCAGCACCAAAGGGGTATGTAACTCCATTCCCCTGGCCAAAGAGCCGTGACTATGTACCATATGCCAATGCACCATATAAGAGCTTGACTGTTGAGAAGGCTGTTCAGAACTGGATCCAATATGAGGGTAACGTATTTCGATTCCCTGGTGGGGGAACACAGTTTCCTCAAGGGGCAGATAAATATATTGATCAGCTTGCTGCTGTGATACCAATAAAGAATGGGACAGTAAGAACTGCACTGGACACCGGTTGTGGGGTTGCCAGTTGGGGTGCATACTTGTTGAGTAGAAATGTTCTTGCCATGTCGTTTGCGCCAAGAGATTCCCATGAGGCACAAGTCCAATTTGCTCTTGAGAGGGGTGTACCTGCAGTTATTGGTGTTCTGGGTACAATAAAGCTGCCCTATCCATCCAGAGCCTTTGACATGGCTCATTGTTCTCGTTGCTTAATTCCATGGGGAATAAATGATGGAAAATATCTCAAGGAAGTTGACCGAGTTCTTAGGCCTGGGGGTTATTGGGTGCTTTCGGGTCCTCCGATCAATTGGAAGAATAATTACCAAGCATGGCAGCGTCCCAAGGAGGATCTTCAGGAAGAACAGAGACAGATTGAAGAAGCTGCTAAACTTCTTTGCTGGGAGAAGAAGTCTGAGAAGGGTGAAACTGCCATTTGGCAGAAGAGGGTAGACTCTGATTCATGTGGAGACAGACAAGATGATTCCCGTGCTAACTTTTGTAAAGCTGATGAAGCAGATAGCGTCTGGTATAAGAAAATGGAGGGATGCATAACTCCATACCCTAAAGTTTCCAGTGGGGAGTTGAAGCCGTTTCCAAAAAGGCTTTATGCTGTTCCCCCCAGGATTTCTAGTGGGTCTGTTCCTGGAGTTTCTGTTGAGGACTACGAGGAGGATAACAACAAATGGAAGAAGCACGTGAATGCTTATAAAAGAATCAATAAACTAATAGACACAGGAAGGTATCGCAACATTATGGATATGAATGCTGGTTTGGGTGGTTTTGCTGCTGCAATTGAATCTCCAAAATTGTGGGTCATGAATGTGATGCCCACAATAGCCGAGAAAAATACGCTGGGTGTTGTATATGAGCGAGGATTGATTGGAATCTACCATGATTGGTGTGAAGGTTTCTCTACTTACCCAAGGACATATGACCTCATTCATGCCCATGGTGTTTTCAGTATGTACAATGGAAAATGCAACTGGGAAGACATTCTTCTAGAGATGGATCGGATCTTGCGGCCAGAAGGTGCCGTCATATTCCGTGATGAAGTCGATGTATTAATTAAGGTGAAGAAGATAGTAGGAGGAATGAGATGGGATACTAAACTGGTGGACCATGAGGATGGTCCCCTTGTACCAGAGAAGGTATTGGTTGCTGTGAAGCAGTACTGGGTTGGAAACTCCACCTCCGCACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

609

Amino Acids

68.64

Weight (kDa)

7.48

Isoelectric Point (pI)

39.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	93 - 597	5.6e-228	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	210 - 304	8.1e-07	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002417)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26850 AT1G26850 AT1G26850
fragaria_vesca	FvH4_4g31440 FvH4_4g31440 FvH4_4g31440 FvH4_5g15080
malus_domestica	MD06G1192300.v1.1 MD13G1072700.v1.1 MD14G1198900.v1.1 MD16G1073500.v1.1
prunus_persica	Prupe.1G273400_v2.0.a1 Prupe.1G273400_v2.0.a1 Prupe.1G273400_v2.0.a1 Prupe.5G192000_v2.0.a1 Prupe.5G192000_v2.0.a1
pyrus_communis	pycom06g17040 pycom13g06520 pycom14g16510 pycom16g06360
rosa_chinensis	RchiOBHm_Chr4g0439921 RchiOBHm_Chr7g0181131
rosa_laevigata	RLG00000005211 RLG00000006225
rosa_multiflora	Rmu_sc0001147.1_g000019 Rmu_sc0022459.1_g000002
rosa_roxburghii	Rroxscaffold_3G00272250 Rroxscaffold_5G00380870
rosa_rugosa	Rorug04G0322400 Rorug04G0322500 Rorug06G0438700
rosa_samantha	Rh4AG373400 Rh4BG385700 Rh4CG400200 Rh4DG380000 Rh7AG040600 Rh7BG040000 Rh7CG042000 Rh7DG040400
rosa_wichuraiana	Rw4G031930 Rw7G003350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1332
Acc36I	ACCTGC	1 cut(s)	763
AccB1I	GGYRCC	1 cut(s)	1655
AccI	GTMKAC	1 cut(s)	1074
AciI	CCGC	2 cut(s)	1645, 1818
AclWI	GGATC	5 cut(s)	496, 509, 981, 1641, 1646
AcoI	YGGCCR	2 cut(s)	422, 1646
AcsI	RAATTY	1 cut(s)	48
AcuI	CTGAAG	1 cut(s)	962
AcvI	CACGTG	1 cut(s)	1321
AdeI	CACNNNGTG	1 cut(s)	288
AfaI	GTAC	6 cut(s)	444, 753, 778, 1592, 1764, 1796
AfiI	CCNNNNNNNGG	5 cut(s)	361, 511, 538, 539, 642
AgeI	ACCGGT	1 cut(s)	635
AgsI	TTSAA	3 cut(s)	1001, 1210, 1421
AjnI	CCWGG	5 cut(s)	419, 532, 895, 1244, 1267
AjuI	GAANNNNNNNTTGG	2 cut(s)	1379, 1411
AloI	GAACNNNNNNTCC	2 cut(s)	535, 567
AluBI	AGCT	7 cut(s)	39, 62, 471, 583, 787, 1007, 1130
AluI	AGCT	7 cut(s)	39, 62, 471, 583, 787, 1007, 1130
Alw21I	GWGCWC	1 cut(s)	41
Alw26I	GTCTC	2 cut(s)	985, 1086
AlwI	GGATC	5 cut(s)	496, 509, 981, 1641, 1646
AoxI	GGCC	3 cut(s)	422, 893, 1646
ApeKI	GCWGC	7 cut(s)	59, 587, 787, 958, 1007, 1409, 1412
ApoI	RAATTY	1 cut(s)	48
AseI	ATTAAT	1 cut(s)	1688
AsiGI	ACCGGT	1 cut(s)	635
Asp700I	GAANNNNTTC	1 cut(s)	1617
AspLEI	GCGC	1 cut(s)	703
AspS9I	GGNCC	3 cut(s)	920, 1741, 1754
AsuHPI	GGTGA	3 cut(s)	218, 1058, 1708
AsuII	TTCGAA	1 cut(s)	252
AvaII	GGWCC	3 cut(s)	920, 1741, 1754
AxyI	CCTNAGG	1 cut(s)	360
BalI	TGGCCA	1 cut(s)	424
BamHI	GGATCC	1 cut(s)	501
BanI	GGYRCC	1 cut(s)	1655
BanII	GRGCYC	1 cut(s)	41
BarI	GAAGNNNNNNTAC	4 cut(s)	357, 389, 929, 961
BbrPI	CACGTG	1 cut(s)	1321
BbsI	GAAGAC	1 cut(s)	1620
Bbv12I	GWGCWC	1 cut(s)	41
BbvI	GCAGC	7 cut(s)	71, 574, 774, 970, 994, 1396, 1399
BccI	CCATC	5 cut(s)	805, 851, 1624, 1716, 1745
BceAI	ACGGC	3 cut(s)	417, 1198, 1643
BciT130I	CCWGG	5 cut(s)	421, 534, 897, 1246, 1269
BciVI	GTATCC	1 cut(s)	1720
BclI	TGATCA	1 cut(s)	577
BcoDI	GTCTC	2 cut(s)	985, 1086
BfaI	CTAG	3 cut(s)	33, 1254, 1625
BfmI	CTRYAG	1 cut(s)	756
BfuAI	ACCTGC	1 cut(s)	763
BfuI	GTATCC	1 cut(s)	1720
BisI	GCNGC	8 cut(s)	60, 588, 788, 959, 1008, 1410, 1413, 1646
BlsI	GCNGC	8 cut(s)	61, 589, 789, 960, 1009, 1411, 1414, 1647
BmcAI	AGTACT	1 cut(s)	1796
Bme1390I	CCNGG	5 cut(s)	421, 534, 897, 1246, 1269
Bme18I	GGWCC	3 cut(s)	920, 1741, 1754
BmgT120I	GGNCC	3 cut(s)	920, 1741, 1754
BmiI	GGNNCC	5 cut(s)	6, 503, 921, 1657, 1756
BmrFI	CCNGG	5 cut(s)	421, 534, 897, 1246, 1269
BmrI	ACTGGG	2 cut(s)	1618, 1807
BmsI	GCATC	2 cut(s)	1160, 1440
BmuI	ACTGGG	2 cut(s)	1618, 1807
BpiI	GAAGAC	1 cut(s)	1620
BpmI	CTGGAG	1 cut(s)	1290
Bpu14I	TTCGAA	1 cut(s)	252
BpuEI	CTTGAG	3 cut(s)	540, 761, 854
Bsa29I	ATCGAT	1 cut(s)	222
BsaAI	YACGTR	1 cut(s)	1321
BsaBI	GATNNNNATC	1 cut(s)	1508
BsaJI	CCNNGG	9 cut(s)	320, 419, 532, 842, 896, 966, 1244, 1547, 1573
BsaWI	WCCGGW	1 cut(s)	635
BsaXI	ACNNNNNCTCC	5 cut(s)	1019, 1049, 1263, 1293, 1799
Bsc4I	CCNNNNNNNGG	5 cut(s)	361, 511, 538, 539, 642
Bse118I	RCCGGY	2 cut(s)	203, 635
Bse1I	ACTGG	7 cut(s)	503, 633, 651, 1197, 1613, 1740, 1802
Bse21I	CCTNAGG	1 cut(s)	360
Bse3DI	GCAATG	3 cut(s)	315, 349, 376
Bse8I	GATNNNNATC	1 cut(s)	1508
BseBI	CCWGG	5 cut(s)	421, 534, 897, 1246, 1269
BseCI	ATCGAT	1 cut(s)	222
BseDI	CCNNGG	9 cut(s)	320, 419, 532, 842, 896, 966, 1244, 1547, 1573
BseGI	GGATG	3 cut(s)	797, 1175, 1756
BseJI	GATNNNNATC	1 cut(s)	1508
BseLI	CCNNNNNNNGG	5 cut(s)	361, 511, 538, 539, 642
BseMI	GCAATG	3 cut(s)	315, 349, 376
BseMII	CTCAG	2 cut(s)	351, 1029
BseNI	ACTGG	7 cut(s)	503, 633, 651, 1197, 1613, 1740, 1802
BseRI	GAGGAG	1 cut(s)	1307
BseXI	GCAGC	7 cut(s)	71, 574, 774, 970, 994, 1396, 1399
BseYI	CCCAGC	2 cut(s)	1023, 1477
BsgI	GTGCAG	1 cut(s)	609
Bsh1285I	CGRYCG	1 cut(s)	28
BshFI	GGCC	3 cut(s)	424, 895, 1648
BshNI	GGYRCC	1 cut(s)	1655
BshTI	ACCGGT	1 cut(s)	635
BshVI	ATCGAT	1 cut(s)	222
BsiEI	CGRYCG	1 cut(s)	28
BsiHKAI	GWGCWC	1 cut(s)	41
BsiSI	CCGG	2 cut(s)	204, 636
BslFI	GGGAC	3 cut(s)	625, 948, 1740
BslI	CCNNNNNNNGG	5 cut(s)	361, 511, 538, 539, 642
BsmAI	GTCTC	2 cut(s)	985, 1086
BsmFI	GGGAC	3 cut(s)	625, 948, 1740
BsmI	GAATGC	3 cut(s)	22, 1330, 1393
BsnI	GGCC	3 cut(s)	424, 895, 1648
Bsp119I	TTCGAA	1 cut(s)	252
Bsp1286I	GDGCHC	1 cut(s)	41
Bsp1407I	TGTACA	1 cut(s)	1590
Bsp143I	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
Bsp19I	CCATGG	2 cut(s)	842, 1573
BspACI	CCGC	2 cut(s)	1645, 1818
BspANI	GGCC	3 cut(s)	424, 895, 1648
BspCNI	CTCAG	2 cut(s)	352, 1030
BspDI	ATCGAT	1 cut(s)	222
BspHI	TCATGA	1 cut(s)	1440
BspLI	GGNNCC	5 cut(s)	6, 503, 921, 1657, 1756
BspMAI	CTGCAG	1 cut(s)	760
BspMI	ACCTGC	1 cut(s)	763
BspPI	GGATC	5 cut(s)	496, 509, 981, 1641, 1646
BspT104I	TTCGAA	1 cut(s)	252
BspT107I	GGYRCC	1 cut(s)	1655
BsrDI	GCAATG	3 cut(s)	315, 349, 376
BsrFI	RCCGGY	2 cut(s)	203, 635
BsrGI	TGTACA	1 cut(s)	1590
BsrI	ACTGG	7 cut(s)	503, 633, 651, 1197, 1613, 1740, 1802
BssAI	RCCGGY	2 cut(s)	203, 635
BssECI	CCNNGG	9 cut(s)	320, 419, 532, 842, 896, 966, 1244, 1547, 1573
BssMI	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
BssT1I	CCWWGG	5 cut(s)	320, 842, 966, 1547, 1573
Bst2UI	CCWGG	5 cut(s)	421, 534, 897, 1246, 1269
Bst4CI	ACNGT	5 cut(s)	26, 258, 478, 549, 616
Bst6I	CTCTTC	1 cut(s)	1061
BstAUI	TGTACA	1 cut(s)	1590
BstBAI	YACGTR	1 cut(s)	1321
BstBI	TTCGAA	1 cut(s)	252
BstC8I	GCNNGC	2 cut(s)	306, 585
BstDEI	CTNAG	5 cut(s)	147, 182, 360, 890, 1038
BstDSI	CCRYGG	2 cut(s)	842, 1573
BstF5I	GGATG	3 cut(s)	797, 1175, 1756
BstHHI	GCGC	1 cut(s)	703
BstKTI	GATC	8 cut(s)	144, 298, 504, 580, 930, 976, 1636, 1641
BstMAI	GTCTC	2 cut(s)	985, 1086
BstMBI	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
BstMCI	CGRYCG	1 cut(s)	28
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 1136
BstNI	CCWGG	5 cut(s)	421, 534, 897, 1246, 1269
BstNSI	RCATGY	1 cut(s)	203
BstSCI	CCNGG	5 cut(s)	419, 532, 895, 1244, 1267
BstSFI	CTRYAG	1 cut(s)	756
BstV1I	GCAGC	7 cut(s)	71, 574, 774, 970, 994, 1396, 1399
BstV2I	GAAGAC	1 cut(s)	1620
BstX2I	RGATCY	3 cut(s)	501, 973, 1638
BstXI	CCANNNNNNTGG	3 cut(s)	420, 1434, 1751
BstYI	RGATCY	3 cut(s)	501, 973, 1638
Bsu15I	ATCGAT	1 cut(s)	222
Bsu36I	CCTNAGG	1 cut(s)	360
BsuI	GTATCC	1 cut(s)	1720
BsuRI	GGCC	3 cut(s)	424, 895, 1648
BsuTUI	ATCGAT	1 cut(s)	222
BtgI	CCRYGG	2 cut(s)	842, 1573
BtsCI	GGATG	3 cut(s)	797, 1175, 1756
BtsIMutI	CAGTG	3 cut(s)	273, 626, 1204
BveI	ACCTGC	1 cut(s)	763
Cac8I	GCNNGC	2 cut(s)	306, 585
CciI	TCATGA	1 cut(s)	1440
CfoI	GCGC	1 cut(s)	703
Cfr10I	RCCGGY	2 cut(s)	203, 635
Cfr13I	GGNCC	3 cut(s)	920, 1741, 1754
ClaI	ATCGAT	1 cut(s)	222
CseI	GACGC	2 cut(s)	950, 1135
Csp6I	GTAC	6 cut(s)	443, 752, 777, 1591, 1763, 1795
CspAI	ACCGGT	1 cut(s)	635
CviQI	GTAC	6 cut(s)	443, 752, 777, 1591, 1763, 1795
DdeI	CTNAG	5 cut(s)	147, 182, 360, 890, 1038
DpnI	GATC	8 cut(s)	143, 297, 503, 579, 929, 975, 1635, 1640
DpnII	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
DraIII	CACNNNGTG	1 cut(s)	288
EaeI	YGGCCR	2 cut(s)	422, 1646
Eam1104I	CTCTTC	1 cut(s)	1061
EarI	CTCTTC	1 cut(s)	1061
Ecl136II	GAGCTC	1 cut(s)	39
Eco130I	CCWWGG	5 cut(s)	320, 842, 966, 1547, 1573
Eco147I	AGGCCT	1 cut(s)	895
Eco24I	GRGCYC	1 cut(s)	41
Eco47I	GGWCC	3 cut(s)	920, 1741, 1754
Eco53kI	GAGCTC	1 cut(s)	39
Eco57I	CTGAAG	1 cut(s)	962
Eco72I	CACGTG	1 cut(s)	1321
Eco81I	CCTNAGG	1 cut(s)	360
EcoICRI	GAGCTC	1 cut(s)	39
EcoO109I	RGGNCCY	1 cut(s)	920
EcoRII	CCWGG	5 cut(s)	419, 532, 895, 1244, 1267
EcoT14I	CCWWGG	5 cut(s)	320, 842, 966, 1547, 1573
EcoT22I	ATGCAT	1 cut(s)	1175
EcoT38I	GRGCYC	1 cut(s)	41
ErhI	CCWWGG	5 cut(s)	320, 842, 966, 1547, 1573
FalI	AAGNNNNNCTT	2 cut(s)	1605, 1637
FaqI	GGGAC	3 cut(s)	625, 948, 1740
FauNDI	CATATG	2 cut(s)	448, 1555
FbaI	TGATCA	1 cut(s)	577
FblI	GTMKAC	1 cut(s)	1074
Fnu4HI	GCNGC	8 cut(s)	60, 588, 788, 959, 1008, 1410, 1413, 1646
FokI	GGATG	3 cut(s)	784, 1182, 1763
FriOI	GRGCYC	1 cut(s)	41
Fsp4HI	GCNGC	8 cut(s)	60, 588, 788, 959, 1008, 1410, 1413, 1646
FspBI	CTAG	3 cut(s)	33, 1254, 1625
GlaI	GCGC	1 cut(s)	702
GluI	GCNGC	8 cut(s)	60, 588, 788, 959, 1008, 1410, 1413, 1646
GsaI	CCCAGC	2 cut(s)	1027, 1481
GsuI	CTGGAG	1 cut(s)	1290
HaeIII	GGCC	3 cut(s)	424, 895, 1648
HapII	CCGG	2 cut(s)	204, 636
HgaI	GACGC	2 cut(s)	950, 1135
HhaI	GCGC	1 cut(s)	703
Hin6I	GCGC	1 cut(s)	701
HinP1I	GCGC	1 cut(s)	701
HincII	GTYRAC	1 cut(s)	880
HindII	GTYRAC	1 cut(s)	880
HpaII	CCGG	2 cut(s)	204, 636
HphI	GGTGA	3 cut(s)	218, 1058, 1708
Hpy166II	GTNNAC	4 cut(s)	752, 880, 1075, 1741
Hpy188I	TCNGA	9 cut(s)	9, 30, 232, 300, 495, 927, 1039, 1081, 1638
Hpy188III	TCNNGA	7 cut(s)	341, 359, 740, 801, 980, 1441, 1625
Hpy8I	GTNNAC	4 cut(s)	752, 880, 1075, 1741
HpyAV	CCTTC	6 cut(s)	478, 1036, 1356, 1523, 1646, 1765
HpyCH4III	ACNGT	5 cut(s)	26, 258, 478, 549, 616
HpyCH4IV	ACGT	2 cut(s)	519, 1320
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 1136
HpyF3I	CTNAG	5 cut(s)	147, 182, 360, 890, 1038
HpySE526I	ACGT	2 cut(s)	519, 1320
HspAI	GCGC	1 cut(s)	701
Ksp22I	TGATCA	1 cut(s)	577
Kzo9I	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
LmnI	GCTCC	2 cut(s)	10, 36
Lsp1109I	GCAGC	7 cut(s)	71, 574, 774, 970, 994, 1396, 1399
LweI	GCATC	2 cut(s)	1160, 1440
MaeI	CTAG	3 cut(s)	33, 1254, 1625
MaeII	ACGT	2 cut(s)	519, 1320
MaeIII	GTNAC	5 cut(s)	269, 372, 406, 434, 515
MalI	GATC	8 cut(s)	143, 297, 503, 579, 929, 975, 1635, 1640
MboI	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
MfeI	CAATTG	2 cut(s)	931, 1416
MflI	RGATCY	3 cut(s)	501, 973, 1638
MhlI	GDGCHC	1 cut(s)	41
MlsI	TGGCCA	1 cut(s)	424
MluCI	AATT	9 cut(s)	48, 334, 731, 837, 931, 943, 1416, 1430, 1689
MluNI	TGGCCA	1 cut(s)	424
MlyI	GAGTC	1 cut(s)	1070
MmeI	TCCRAC	3 cut(s)	8, 193, 1783
Mox20I	TGGCCA	1 cut(s)	424
Mph1103I	ATGCAT	1 cut(s)	1175
MroXI	GAANNNNTTC	1 cut(s)	1617
MscI	TGGCCA	1 cut(s)	424
MseI	TTAA	3 cut(s)	836, 1688, 1692
MslI	CAYNNNNRTG	5 cut(s)	204, 1323, 1445, 1572, 1749
Msp20I	TGGCCA	1 cut(s)	424
MspA1I	CMGCKG	1 cut(s)	62
MspI	CCGG	2 cut(s)	204, 636
MspR9I	CCNGG	5 cut(s)	421, 534, 897, 1246, 1269
MunI	CAATTG	2 cut(s)	931, 1416
Mva1269I	GAATGC	3 cut(s)	22, 1330, 1393
MvaI	CCWGG	5 cut(s)	421, 534, 897, 1246, 1269
MwoI	GCNNNNNNNGC	2 cut(s)	11, 1136
NcoI	CCATGG	2 cut(s)	842, 1573
NdeI	CATATG	2 cut(s)	448, 1555
NdeII	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
NlaIV	GGNNCC	5 cut(s)	6, 503, 921, 1657, 1756
NmeAIII	GCCGAG	1 cut(s)	1489
NmuCI	GTSAC	1 cut(s)	434
NsiI	ATGCAT	1 cut(s)	1175
NspI	RCATGY	1 cut(s)	203
NspV	TTCGAA	1 cut(s)	252
PacI	TTAATTAA	1 cut(s)	1692
PagI	TCATGA	1 cut(s)	1440
PceI	AGGCCT	1 cut(s)	895
PcsI	WCGNNNNNNNCGW	1 cut(s)	923
PctI	GAATGC	3 cut(s)	22, 1330, 1393
PdmI	GAANNNNTTC	1 cut(s)	1617
PfoI	TCCNGGA	1 cut(s)	1267
PinAI	ACCGGT	1 cut(s)	635
PkrI	GCNGC	8 cut(s)	61, 589, 789, 960, 1009, 1411, 1414, 1647
PleI	GAGTC	1 cut(s)	1070
PmaCI	CACGTG	1 cut(s)	1321
PmlI	CACGTG	1 cut(s)	1321
PpsI	GAGTC	1 cut(s)	1070
Ppu21I	YACGTR	1 cut(s)	1321
PpuMI	RGGWCCY	1 cut(s)	920
PshBI	ATTAAT	1 cut(s)	1688
PsiI	TTATAA	1 cut(s)	1332
Psp124BI	GAGCTC	1 cut(s)	41
Psp5II	RGGWCCY	1 cut(s)	920
Psp6I	CCWGG	5 cut(s)	419, 532, 895, 1244, 1267
PspCI	CACGTG	1 cut(s)	1321
PspFI	CCCAGC	2 cut(s)	1023, 1477
PspGI	CCWGG	5 cut(s)	419, 532, 895, 1244, 1267
PspN4I	GGNNCC	5 cut(s)	6, 503, 921, 1657, 1756
PspPI	GGNCC	3 cut(s)	920, 1741, 1754
PspPPI	RGGWCCY	1 cut(s)	920
PstI	CTGCAG	1 cut(s)	760
PsuI	RGATCY	3 cut(s)	501, 973, 1638
PvuII	CAGCTG	1 cut(s)	62
RsaI	GTAC	6 cut(s)	444, 753, 778, 1592, 1764, 1796
RsaNI	GTAC	6 cut(s)	443, 752, 777, 1591, 1763, 1795
RseI	CAYNNNNRTG	5 cut(s)	204, 1323, 1445, 1572, 1749
SacI	GAGCTC	1 cut(s)	41
SaqAI	TTAA	3 cut(s)	836, 1688, 1692
SatI	GCNGC	8 cut(s)	60, 588, 788, 959, 1008, 1410, 1413, 1646
Sau3AI	GATC	8 cut(s)	141, 295, 501, 577, 927, 973, 1633, 1638
Sau96I	GGNCC	3 cut(s)	920, 1741, 1754
ScaI	AGTACT	1 cut(s)	1796
SchI	GAGTC	1 cut(s)	1070
ScrFI	CCNGG	5 cut(s)	421, 534, 897, 1246, 1269
SduI	GDGCHC	1 cut(s)	41
SfaNI	GCATC	2 cut(s)	1160, 1440
SfcI	CTRYAG	1 cut(s)	756
SfuI	TTCGAA	1 cut(s)	252
SinI	GGWCC	3 cut(s)	920, 1741, 1754
SmiMI	CAYNNNNRTG	5 cut(s)	204, 1323, 1445, 1572, 1749
SmlI	CTYRAG	3 cut(s)	555, 740, 869
SmoI	CTYRAG	3 cut(s)	555, 740, 869
Sse9I	AATT	9 cut(s)	48, 334, 731, 837, 931, 943, 1416, 1430, 1689
SseBI	AGGCCT	1 cut(s)	895
SsiI	CCGC	2 cut(s)	1645, 1818
SspI	AATATT	1 cut(s)	169
SspMI	CTAG	3 cut(s)	33, 1254, 1625
SstI	GAGCTC	1 cut(s)	41
StuI	AGGCCT	1 cut(s)	895
StyD4I	CCNGG	5 cut(s)	419, 532, 895, 1244, 1267
StyI	CCWWGG	5 cut(s)	320, 842, 966, 1547, 1573
TaaI	ACNGT	5 cut(s)	26, 258, 478, 549, 616
TaiI	ACGT	2 cut(s)	522, 1323
TaqI	TCGA	6 cut(s)	222, 236, 252, 340, 526, 1680
TaqII	GACCGA	1 cut(s)	897
TasI	AATT	9 cut(s)	48, 334, 731, 837, 931, 943, 1416, 1430, 1689
TatI	WGTACW	2 cut(s)	1590, 1794
TauI	GCSGC	1 cut(s)	1648
Tru1I	TTAA	3 cut(s)	836, 1688, 1692
Tru9I	TTAA	3 cut(s)	836, 1688, 1692
TscAI	CASTG	3 cut(s)	280, 633, 1204
TseFI	GTSAC	1 cut(s)	434
TseI	GCWGC	7 cut(s)	59, 587, 787, 958, 1007, 1409, 1412
Tsp45I	GTSAC	1 cut(s)	434
TspDTI	ATGAA	8 cut(s)	240, 347, 1074, 1149, 1400, 1457, 1556, 1689
TspGWI	ACGGA	1 cut(s)	1658
TspRI	CASTG	3 cut(s)	280, 633, 1204
VpaK11BI	GGWCC	3 cut(s)	920, 1741, 1754
VspI	ATTAAT	1 cut(s)	1688
XapI	RAATTY	1 cut(s)	48
XbaI	TCTAGA	1 cut(s)	1624
XceI	RCATGY	1 cut(s)	203
XmiI	GTMKAC	1 cut(s)	1074
XmnI	GAANNNNTTC	1 cut(s)	1617
XspI	CTAG	3 cut(s)	33, 1254, 1625
ZrmI	AGTACT	1 cut(s)	1796
Zsp2I	ATGCAT	1 cut(s)	1175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.