MD13G1074900.v1.1

Bacterial PH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015409
Physical Location & Seq
Forward (+)
5284553 .. 5285161
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1074900.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCCTCCACCCCAATCCTCTCCCCCACCACAACCACCACCACGTCCTCCTTAGTCCACCACCACCACCAATCTCTACACTTCTCTAAATTACCCTTTCCCAACTCCCTCACCAAACCCAGCACTCTCAATTCCAACAGACCACTTACCAGACTTCACGTGTCCACCCCAACTAACAAACCCAGCAACACCACCGCCACCTCCACCTCCAAGAAACCCACGACGGAGACCATCTTCTTCGACGGCGGAGCTCACTACGGCGACCTGCTGGCCAACCTTATCCTGGGTTTCACTCTTCTCTGGCTGCCCTTGACTTTAGCTGCGGTCTTCAGGGCCTTCTTTTTGAGGTACAGGTTCACCAACCTGCGTGTGACGGTAATCTCGGGACTGACGGGTCAGGACAGGAGCGACTTCTCGTACAAGGTGATCAAGGATGTCCAGGTGGTGCCGCGTTTCATCGGCGAATGGGGTGATATTATCATCACCCTCAAAGACGGCACCAAGGTTGACCTGAGGAGTGTGCCCAAGTTCAGAGAGATTGCCAAGTATTGCCTCTCTTTGGCGGAAAAGCCGGCCGTATTGAAGGAAACGGGAACCAAAGGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bPH_2

Protein Analysis

203

Amino Acids

22.41

Weight (kDa)

10.0

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bPH_2 PF03703 114 - 184 2.8e-13 Bacterial PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14345
fragaria_vesca FvH4_4g31650
malus_domestica MD13G1074900.v1.1
prunus_persica Prupe.1G271300_v2.0.a1
pyrus_communis pycom13g06670
rosa_chinensis RchiOBHm_Chr4g0440151
rosa_laevigata RLG00000006201
rosa_multiflora Rmu_co8332745.1_g000001 Rmu_sc0001685.1_g000020
rosa_roxburghii Rroxscaffold_5G00381080
rosa_rugosa Rorug04G0324800
rosa_samantha Rh4AG375600 Rh4BG388000 Rh4CG402600 Rh4DG382200
rosa_wichuraiana Rw4G032140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 393
Acc36I ACCTGC 2 cut(s) 273, 372
AccB1I GGYRCC 2 cut(s) 445, 497
AccII CGCG 1 cut(s) 451
AciI CCGC 5 cut(s) 195, 246, 323, 449, 563
AcoI YGGCCR 2 cut(s) 270, 573
AcuI CTGAAG 1 cut(s) 313
AcvI CACGTG 1 cut(s) 160
AfaI GTAC 2 cut(s) 350, 419
AfiI CCNNNNNNNGG 2 cut(s) 283, 559
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 1 cut(s) 583
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 2 cut(s) 282, 438
AluBI AGCT 2 cut(s) 251, 320
AluI AGCT 2 cut(s) 251, 320
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 382
AoxI GGCC 4 cut(s) 3, 270, 333, 573
ApeKI GCWGC 2 cut(s) 304, 320
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 5 cut(s) 103, 349, 436, 475, 482
AvaI CYCGRG 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 512
BaeGI GKGCMC 1 cut(s) 525
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 2 cut(s) 445, 497
BanII GRGCYC 1 cut(s) 253
BbrPI CACGTG 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 2 cut(s) 291, 307
BccI CCATC 1 cut(s) 239
BceAI ACGGC 4 cut(s) 259, 274, 511, 560
BciT130I CCWGG 2 cut(s) 284, 440
BclI TGATCA 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 221
BfuAI ACCTGC 2 cut(s) 273, 372
BisI GCNGC 3 cut(s) 305, 321, 449
BlsI GCNGC 3 cut(s) 306, 322, 450
Bme1390I CCNGG 2 cut(s) 284, 440
BmeT110I CYCGRG 1 cut(s) 382
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 3 cut(s) 447, 499, 595
BmrFI CCNGG 2 cut(s) 284, 440
BpiI GAAGAC 1 cut(s) 319
BsaAI YACGTR 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 283, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 559
Bse118I RCCGGY 1 cut(s) 571
Bse21I CCTNAGG 1 cut(s) 512
BseBI CCWGG 2 cut(s) 284, 440
BseDI CCNNGG 2 cut(s) 283, 501
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 2 cut(s) 283, 559
BseMII CTCAG 1 cut(s) 503
BseRI GAGGAG 1 cut(s) 529
BseSI GKGCMC 1 cut(s) 525
BseX3I CGGCCG 1 cut(s) 573
BseXI GCAGC 2 cut(s) 291, 307
BseYI CCCAGC 2 cut(s) 119, 182
Bsh1236I CGCG 1 cut(s) 451
Bsh1285I CGRYCG 1 cut(s) 576
BshFI GGCC 4 cut(s) 5, 272, 335, 575
BshNI GGYRCC 2 cut(s) 445, 497
BsiEI CGRYCG 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 253
BsiHKCI CYCGRG 1 cut(s) 382
BsiSI CCGG 1 cut(s) 572
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 2 cut(s) 283, 559
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 399
BsnI GGCC 4 cut(s) 5, 272, 335, 575
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 382
Bsp1286I GDGCHC 2 cut(s) 253, 525
Bsp143I GATC 1 cut(s) 426
BspACI CCGC 5 cut(s) 195, 246, 323, 449, 563
BspANI GGCC 4 cut(s) 5, 272, 335, 575
BspCNI CTCAG 1 cut(s) 504
BspFNI CGCG 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 447, 499, 595
BspMI ACCTGC 2 cut(s) 273, 372
BspT107I GGYRCC 2 cut(s) 445, 497
BspTNI GGTCTC 1 cut(s) 221
BsrFI RCCGGY 1 cut(s) 571
BssAI RCCGGY 1 cut(s) 571
BssECI CCNNGG 2 cut(s) 283, 501
BssMI GATC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 2 cut(s) 284, 440
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 300
BstBAI YACGTR 1 cut(s) 160
BstC8I GCNNGC 2 cut(s) 270, 573
BstDEI CTNAG 2 cut(s) 52, 512
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 1 cut(s) 451
BstKTI GATC 1 cut(s) 429
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 1 cut(s) 426
BstMCI CGRYCG 1 cut(s) 576
BstNI CCWGG 2 cut(s) 284, 440
BstSCI CCNGG 2 cut(s) 282, 438
BstSLI GKGCMC 1 cut(s) 525
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 2 cut(s) 291, 307
BstV2I GAAGAC 1 cut(s) 319
BstZI CGGCCG 1 cut(s) 573
Bsu36I CCTNAGG 1 cut(s) 512
BsuRI GGCC 4 cut(s) 5, 272, 335, 575
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 1 cut(s) 439
BveI ACCTGC 2 cut(s) 273, 372
Cac8I GCNNGC 2 cut(s) 270, 573
Cfr10I RCCGGY 1 cut(s) 571
Cfr13I GGNCC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 349, 418
CviJI RGCY 9 cut(s) 5, 251, 272, 304, 320, 335, 571, 575, 603
CviKI_1 RGCY 9 cut(s) 5, 251, 272, 304, 320, 335, 571, 575, 603
CviQI GTAC 2 cut(s) 349, 418
DdeI CTNAG 2 cut(s) 52, 512
DpnI GATC 1 cut(s) 428
DpnII GATC 1 cut(s) 426
DrdI GACNNNNNNGTC 1 cut(s) 393
DseDI GACNNNNNNGTC 1 cut(s) 393
EaeI YGGCCR 2 cut(s) 270, 573
EagI CGGCCG 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
EciI GGCGGA 2 cut(s) 261, 578
Ecl136II GAGCTC 1 cut(s) 251
EclXI CGGCCG 1 cut(s) 573
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 221
Eco52I CGGCCG 1 cut(s) 573
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 313
Eco72I CACGTG 1 cut(s) 160
Eco81I CCTNAGG 1 cut(s) 512
Eco88I CYCGRG 1 cut(s) 382
EcoICRI GAGCTC 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 282, 438
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 501
FaqI GGGAC 1 cut(s) 399
FbaI TGATCA 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 305, 321, 449
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 3 cut(s) 305, 321, 449
GluI GCNGC 3 cut(s) 305, 321, 449
GsaI CCCAGC 2 cut(s) 123, 186
HaeIII GGCC 4 cut(s) 5, 272, 335, 575
HapII CCGG 1 cut(s) 572
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HpaII CCGG 1 cut(s) 572
HphI GGTGA 5 cut(s) 103, 349, 436, 475, 482
Hpy166II GTNNAC 4 cut(s) 58, 165, 357, 508
Hpy188I TCNGA 1 cut(s) 533
Hpy188III TCNNGA 2 cut(s) 384, 398
Hpy8I GTNNAC 4 cut(s) 58, 165, 357, 508
Hpy99I CGWCG 2 cut(s) 226, 245
HpyAV CCTTC 2 cut(s) 346, 577
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 2 cut(s) 44, 159
HpyF3I CTNAG 2 cut(s) 52, 512
HpySE526I ACGT 2 cut(s) 44, 159
KroI GCCGGC 1 cut(s) 571
KroNI GCCGGC 1 cut(s) 573
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 1 cut(s) 426
LmnI GCTCC 2 cut(s) 248, 405
Lsp1109I GCAGC 2 cut(s) 291, 307
MaeII ACGT 2 cut(s) 44, 159
MaeIII GTNAC 1 cut(s) 370
MalI GATC 1 cut(s) 428
MboI GATC 1 cut(s) 426
MboII GAAGA 4 cut(s) 226, 229, 287, 319
MhlI GDGCHC 2 cut(s) 253, 525
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 2 cut(s) 89, 130
MluNI TGGCCA 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 159
Mox20I TGGCCA 1 cut(s) 272
MroNI GCCGGC 1 cut(s) 571
MscI TGGCCA 1 cut(s) 272
MseI TTAA 1 cut(s) 607
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 2 cut(s) 284, 440
MvaI CCWGG 2 cut(s) 284, 440
MvnI CGCG 1 cut(s) 451
NaeI GCCGGC 1 cut(s) 573
NdeII GATC 1 cut(s) 426
NgoMIV GCCGGC 1 cut(s) 571
NlaIV GGNNCC 3 cut(s) 447, 499, 595
NmuCI GTSAC 1 cut(s) 370
PdiI GCCGGC 1 cut(s) 573
PkrI GCNGC 3 cut(s) 306, 322, 450
PmaCI CACGTG 1 cut(s) 160
PmlI CACGTG 1 cut(s) 160
Ppu21I YACGTR 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 282, 438
PspCI CACGTG 1 cut(s) 160
PspFI CCCAGC 2 cut(s) 119, 182
PspGI CCWGG 2 cut(s) 282, 438
PspN4I GGNNCC 3 cut(s) 447, 499, 595
PspPI GGNCC 1 cut(s) 333
RsaI GTAC 2 cut(s) 350, 419
RsaNI GTAC 2 cut(s) 349, 418
SacI GAGCTC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 607
SatI GCNGC 3 cut(s) 305, 321, 449
Sau3AI GATC 1 cut(s) 426
Sau96I GGNCC 1 cut(s) 333
ScrFI CCNGG 2 cut(s) 284, 440
SduI GDGCHC 2 cut(s) 253, 525
Sse9I AATT 2 cut(s) 89, 130
SsiI CCGC 5 cut(s) 195, 246, 323, 449, 563
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 2 cut(s) 282, 438
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 2 cut(s) 47, 162
TaqI TCGA 1 cut(s) 240
TasI AATT 2 cut(s) 89, 130
TauI GCSGC 1 cut(s) 451
Tru1I TTAA 1 cut(s) 607
Tru9I TTAA 1 cut(s) 607
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 2 cut(s) 304, 320
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 445
TspGWI ACGGA 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.