Rh4DG382200

Bacterial PH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015409
Physical Location & Seq
Forward (+)
59965145 .. 59965747
603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG382200.1

Sequence Viewer

Length: 603 bp
ATGGCCTCCTCCTCCTCCACCTCAATTCTCTCACCCACTACCACCACCACCACCACCGCCACCTCCATAGTCCGCCACCACTCCTCCAAGCTCCCCTTCCCTTACTCCACCAAACCCATTTCCACTACTTTCTCTAACAACAGACCACTCACCAGACTCCACGTGTCCACCCCGACCAACAAGCCCGCCAATAACACCGCCAACAAGAAACCCACCTCGGAGACCGTCTTCTTCGACGGCGGGGCCCACTACGGCGACCTCGCGGCCAACCTCGTCCTGGGCTTCACTCTCCTCTGGCTGCCCCTAACGTTAGCCGCCGTGTTCCGGGCGTTTTTCCTGAGGTACAGGTTCACCAACCTGCGGGTGACGGTGATATCGGGACTGACGGGCCAGGACAGGAGTGACTTCTCGTACAAGGTCATCAAGGACGTTCAGGTGGTGCCACGTTTCATTGGCGAGTGGGGTGATATTATCATCACCCTCAGAGATGGCACCAAGGTCGATCTTAGAAGTGTGCCCAAGTTCAGAGAGATTGCCAAGTATTGCTTATCCATGGCTGATAAGCCTGCCGTGGTTAAAGAAACAGGAACTAAGGGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bPH_2

Protein Analysis

200

Amino Acids

22.05

Weight (kDa)

10.09

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bPH_2 PF03703 112 - 182 4.9e-13 Bacterial PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14345
fragaria_vesca FvH4_4g31650
malus_domestica MD13G1074900.v1.1
prunus_persica Prupe.1G271300_v2.0.a1
pyrus_communis pycom13g06670
rosa_chinensis RchiOBHm_Chr4g0440151
rosa_laevigata RLG00000006201
rosa_multiflora Rmu_co8332745.1_g000001 Rmu_sc0001685.1_g000020
rosa_roxburghii Rroxscaffold_5G00381080
rosa_rugosa Rorug04G0324800
rosa_samantha Rh4AG375600 Rh4BG388000 Rh4CG402600 Rh4DG382200
rosa_wichuraiana Rw4G032140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 366
AccB1I GGYRCC 2 cut(s) 439, 491
AccII CGCG 1 cut(s) 263
AciI CCGC 8 cut(s) 57, 73, 186, 198, 240, 263, 315, 361
AclI AACGTT 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 264
AcvI CACGTG 1 cut(s) 163
AfaI GTAC 2 cut(s) 344, 413
AfiI CCNNNNNNNGG 3 cut(s) 277, 324, 360
AflIII ACRYGT 1 cut(s) 162
AjnI CCWGG 2 cut(s) 276, 390
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 1 cut(s) 91
AluI AGCT 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 215
AoxI GGCC 4 cut(s) 3, 243, 264, 388
ApaI GGGCCC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 298
AspS9I GGNCC 3 cut(s) 243, 244, 388
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 7 cut(s) 24, 142, 343, 376, 382, 469, 476
AxyI CCTNAGG 1 cut(s) 338
BaeGI GKGCMC 2 cut(s) 247, 519
BanI GGYRCC 2 cut(s) 439, 491
BanII GRGCYC 1 cut(s) 247
BbrPI CACGTG 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 285
BccI CCATC 1 cut(s) 482
BceAI ACGGC 4 cut(s) 253, 268, 302, 554
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 2 cut(s) 278, 392
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 366
BisI GCNGC 3 cut(s) 264, 299, 315
BlsI GCNGC 3 cut(s) 265, 300, 316
Bme1390I CCNGG 3 cut(s) 278, 326, 392
BmgT120I GGNCC 3 cut(s) 243, 244, 388
BmiI GGNNCC 4 cut(s) 244, 245, 441, 493
BmrFI CCNGG 3 cut(s) 278, 326, 392
BpiI GAAGAC 1 cut(s) 220
BpuMI CCSGG 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 5 cut(s) 216, 277, 495, 552, 570
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 324, 360
Bse21I CCTNAGG 1 cut(s) 338
BseBI CCWGG 2 cut(s) 278, 392
BseDI CCNNGG 5 cut(s) 216, 277, 495, 552, 570
BseLI CCNNNNNNNGG 3 cut(s) 277, 324, 360
BseMII CTCAG 2 cut(s) 329, 496
BseRI GAGGAG 3 cut(s) 4, 73, 281
BseSI GKGCMC 2 cut(s) 247, 519
BseXI GCAGC 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 4 cut(s) 5, 245, 266, 390
BshNI GGYRCC 2 cut(s) 439, 491
BsiSI CCGG 1 cut(s) 325
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 3 cut(s) 277, 324, 360
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 393
BsnI GGCC 4 cut(s) 5, 245, 266, 390
Bso31I GGTCTC 1 cut(s) 215
Bsp120I GGGCCC 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 247, 519
Bsp143I GATC 1 cut(s) 502
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 8 cut(s) 57, 73, 186, 198, 240, 263, 315, 361
BspANI GGCC 4 cut(s) 5, 245, 266, 390
BspCNI CTCAG 2 cut(s) 330, 495
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 4 cut(s) 244, 245, 441, 493
BspMI ACCTGC 1 cut(s) 366
BspT107I GGYRCC 2 cut(s) 439, 491
BspTNI GGTCTC 1 cut(s) 215
BssECI CCNNGG 5 cut(s) 216, 277, 495, 552, 570
BssMI GATC 1 cut(s) 502
BssT1I CCWWGG 2 cut(s) 495, 552
Bst2UI CCWGG 2 cut(s) 278, 392
Bst4CI ACNGT 2 cut(s) 226, 370
BstBAI YACGTR 1 cut(s) 163
BstC8I GCNNGC 2 cut(s) 186, 567
BstDEI CTNAG 4 cut(s) 338, 482, 506, 591
BstDSI CCRYGG 2 cut(s) 552, 570
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 1 cut(s) 502
BstNI CCWGG 2 cut(s) 278, 392
BstSCI CCNGG 3 cut(s) 276, 324, 390
BstSLI GKGCMC 2 cut(s) 247, 519
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 220
Bsu36I CCTNAGG 1 cut(s) 338
BsuRI GGCC 4 cut(s) 5, 245, 266, 390
BtgI CCRYGG 2 cut(s) 552, 570
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 186, 567
Cfr13I GGNCC 3 cut(s) 243, 244, 388
Csp6I GTAC 2 cut(s) 343, 412
CviAII CATG 1 cut(s) 553
CviQI GTAC 2 cut(s) 343, 412
DdeI CTNAG 4 cut(s) 338, 482, 506, 591
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
EaeI YGGCCR 1 cut(s) 264
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 2 cut(s) 495, 552
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 215
Eco32I GATATC 1 cut(s) 375
Eco72I CACGTG 1 cut(s) 163
Eco81I CCTNAGG 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 243
EcoRII CCWGG 2 cut(s) 276, 390
EcoRV GATATC 1 cut(s) 375
EcoT14I CCWWGG 2 cut(s) 495, 552
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 2 cut(s) 495, 552
FaeI CATG 1 cut(s) 556
FaiI YATR 2 cut(s) 68, 554
FalI AAGNNNNNCTT 4 cut(s) 80, 112, 530, 562
FaqI GGGAC 1 cut(s) 393
FatI CATG 1 cut(s) 552
FauI CCCGC 3 cut(s) 193, 233, 354
Fnu4HI GCNGC 3 cut(s) 264, 299, 315
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 3 cut(s) 264, 299, 315
GluI GCNGC 3 cut(s) 264, 299, 315
HaeIII GGCC 4 cut(s) 5, 245, 266, 390
HapII CCGG 1 cut(s) 325
Hin1II CATG 1 cut(s) 556
HinfI GANTC 1 cut(s) 156
HpaII CCGG 1 cut(s) 325
HphI GGTGA 7 cut(s) 24, 142, 343, 376, 382, 469, 476
Hpy166II GTNNAC 2 cut(s) 168, 351
Hpy188I TCNGA 3 cut(s) 220, 485, 527
Hpy188III TCNNGA 2 cut(s) 337, 378
Hpy8I GTNNAC 2 cut(s) 168, 351
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 2 cut(s) 226, 370
HpyCH4IV ACGT 4 cut(s) 162, 308, 429, 445
HpyF3I CTNAG 4 cut(s) 338, 482, 506, 591
HpySE526I ACGT 4 cut(s) 162, 308, 429, 445
Hsp92II CATG 1 cut(s) 556
Kzo9I GATC 1 cut(s) 502
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 1 cut(s) 285
MaeII ACGT 4 cut(s) 162, 308, 429, 445
MaeIII GTNAC 2 cut(s) 364, 401
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 2 cut(s) 220, 223
MhlI GDGCHC 2 cut(s) 247, 519
MluCI AATT 1 cut(s) 24
MlyI GAGTC 1 cut(s) 150
MseI TTAA 1 cut(s) 576
MspI CCGG 1 cut(s) 325
MspR9I CCNGG 3 cut(s) 278, 326, 392
MvaI CCWGG 2 cut(s) 278, 392
MvnI CGCG 1 cut(s) 263
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 552
NdeII GATC 1 cut(s) 502
NlaIII CATG 1 cut(s) 556
NlaIV GGNNCC 4 cut(s) 244, 245, 441, 493
NmuCI GTSAC 2 cut(s) 364, 401
PkrI GCNGC 3 cut(s) 265, 300, 316
PleI GAGTC 1 cut(s) 150
PmaCI CACGTG 1 cut(s) 163
PmlI CACGTG 1 cut(s) 163
PpsI GAGTC 1 cut(s) 150
Ppu21I YACGTR 1 cut(s) 163
Psp1406I AACGTT 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 276, 390
PspCI CACGTG 1 cut(s) 163
PspGI CCWGG 2 cut(s) 276, 390
PspN4I GGNNCC 4 cut(s) 244, 245, 441, 493
PspOMI GGGCCC 1 cut(s) 243
PspPI GGNCC 3 cut(s) 243, 244, 388
RsaI GTAC 2 cut(s) 344, 413
RsaNI GTAC 2 cut(s) 343, 412
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 3 cut(s) 264, 299, 315
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 3 cut(s) 243, 244, 388
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 3 cut(s) 278, 326, 392
SduI GDGCHC 2 cut(s) 247, 519
Sse9I AATT 1 cut(s) 24
SsiI CCGC 8 cut(s) 57, 73, 186, 198, 240, 263, 315, 361
StyD4I CCNGG 3 cut(s) 276, 324, 390
StyI CCWWGG 2 cut(s) 495, 552
TaaI ACNGT 2 cut(s) 226, 370
TaiI ACGT 4 cut(s) 165, 311, 432, 448
TaqI TCGA 2 cut(s) 234, 501
TasI AATT 1 cut(s) 24
TauI GCSGC 2 cut(s) 266, 317
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TseFI GTSAC 2 cut(s) 364, 401
TseI GCWGC 1 cut(s) 298
Tsp45I GTSAC 2 cut(s) 364, 401
TspDTI ATGAA 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.