MD13G1117100.v1.1

ribosome biogenesis GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010219
Physical Location & Seq
Forward (+)
8570084 .. 8573115
3032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1117100.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGCCAATTTCCTCCATGTCCATCCTCCGCCACCGCACCATCATCCCCACCACCGCCGCCGCCCTCCCAACCGTCGCCAAAATCATCCGCCAAATCTCAACTCTCCGGTGCGTCTCCGTCGCCGCAGCCAAACAGCACCAGAACCCTAACGTCTCCAGGAAATCCCAGCCTCCGAACAAGAAGCTCCTCAGAGCCAAACACACCTTCAAGGACTACTCCTCCCTCGCCCCGGTCCTCTCTCCCCAGGACAAGCCCCCGCTCTACGAGTCCCAGACCGTCGGCACGGTCGCCGCTGCCCAAGCCAACTTCATGCGCGTCATCGTGGAGCCTCCCAGAAGCATCGAAGACAGCAAAGTGGAGCTGCTGTGCGTCGTGAAGGCGGTGCTGAAGAAAATTAAGAGGAGAGTGCTGGTTGGGGACAAGGTGCTGGTACGGTCGATTGATTGGGTGGACCGCCGGGGCATGATCGAGAATGTGTACCAGCGGAGCTCCGAGATTCTTGACCCTCCGGTTGCGAATGTGGACCATCTGCTCGTGCTCTTCTCCATGGAGCAGCCCAAGCTCGAGCCGTTCGCGCTCACCAGGTTCTTGATCGAGGCCGAGTCCACCGGAATTCCGCTCACACTCGCTCTCAACAAGTCTGAGCTTGTTGACGAAGAGGCAAAGGCTGCGTGGAAGTTTCGGCTGCGTAAGTGGGGCTACAAGCCCCTTTTTTGCAGTGTTGAATCGAAACATGGACTTGATTCTCTTGCATTTATATTGAGAGATCAAACGAGTGTCATTGTCGGTCCAAGTGGAGTTGGCAAGTCTAGTCTGATTAATGCTTTGAGGAGCAACCACCATGCTTCTGATGCTGCAGAAGGGGAAAGTTTGTTTGATCCAATTTCAGGCAGCAAGTGGTATGGGGACCAGCTTGTTGGACAGGTTTCAACAAGAAGTGGCAGAGGGAAGCACACTACACGCAATGTCTCGTTGCTTCCATTGTCTAACGGGGGTTATCTTGCTGACACTCCTGGCTTCAACCAGCCTAGTTTAACGAAAGTCACCAAGCAATCTCTAGCACAAACATTCCCAGAGATCAGGAAGATGCTCAGTGACAGTGAGCCTGCAAAATGCTCGTTCAATGATTGCCTGCATCTTGGTGAGCCGGGGTGCGTGGTTAGTGCTGATTGGGAAAGATATCCATACTATTTCCAACTTCTTGATGAGATCAGAATCAGAGAGGAATTTCAGTTGAGGACGTACGGAACCAAAAAGGAGGGTGATGTAAGGTACAAGGCGGGAGATAAGGGCGTTCAACAAGCTGAACCACGGTTGGAGCTGAAGAAGCACAGGAGAGTATCTCGCAAGAGTATGAACCAATCTTTACTCGATGAATTAGACGAGCTGGATGATGACAACTTACTAGATGAGGAAAATGATGCGTTTCTCAAGGCAACGAGGGATGAAAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
RsgA_GTPase

Protein Analysis

486

Amino Acids

54.33

Weight (kDa)

9.06

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsgA_GTPase PF03193 160 - 345 3.2e-46 RsgA GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 259, 619
AccII CGCG 2 cut(s) 315, 575
AclWI GGATC 1 cut(s) 872
AcsI RAATTY 2 cut(s) 612, 1226
AcuI CTGAAG 2 cut(s) 407, 1343
AfaI GTAC 4 cut(s) 432, 479, 1244, 1274
AfiI CCNNNNNNNGG 2 cut(s) 229, 887
AgsI TTSAA 6 cut(s) 208, 725, 930, 1021, 1123, 1298
AjnI CCWGG 4 cut(s) 155, 243, 581, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 9 cut(s) 184, 361, 489, 562, 646, 913, 1304, 1321, 1387
AluI AGCT 9 cut(s) 184, 361, 489, 562, 646, 913, 1304, 1321, 1387
Alw21I GWGCWC 2 cut(s) 491, 540
Alw26I GTCTC 3 cut(s) 118, 157, 973
AlwI GGATC 1 cut(s) 872
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 1 cut(s) 597
ApeKI GCWGC 8 cut(s) 125, 293, 361, 553, 668, 685, 854, 891
ApoI RAATTY 2 cut(s) 612, 1226
AseI ATTAAT 1 cut(s) 819
AspLEI GCGC 2 cut(s) 315, 577
AspS9I GGNCC 5 cut(s) 232, 451, 523, 788, 907
AsuC2I CCSGG 3 cut(s) 230, 458, 1149
AsuHPI GGTGA 4 cut(s) 571, 1036, 1154, 1274
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 5 cut(s) 232, 451, 523, 788, 907
BanII GRGCYC 1 cut(s) 491
BauI CACGAG 1 cut(s) 533
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 2 cut(s) 491, 540
BbvI GCAGC 8 cut(s) 137, 280, 348, 565, 655, 672, 841, 903
BccI CCATC 3 cut(s) 29, 47, 534
BceAI ACGGC 1 cut(s) 553
BcgI CGANNNNNNTGC 2 cut(s) 1098, 1132
BciT130I CCWGG 4 cut(s) 157, 245, 583, 1014
BcnI CCSGG 3 cut(s) 230, 458, 1149
BcoDI GTCTC 3 cut(s) 118, 157, 973
BfaI CTAG 4 cut(s) 810, 1029, 1058, 1406
BfmI CTRYAG 1 cut(s) 855
Bme1390I CCNGG 7 cut(s) 157, 230, 245, 458, 583, 1014, 1149
Bme18I GGWCC 5 cut(s) 232, 451, 523, 788, 907
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 5 cut(s) 232, 451, 523, 788, 907
BmiI GGNNCC 3 cut(s) 327, 908, 1249
BmrFI CCNGG 7 cut(s) 157, 230, 245, 458, 583, 1014, 1149
BmsI GCATC 5 cut(s) 348, 841, 1077, 1144, 1411
BpiI GAAGAC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 1327, 1359
BpmI CTGGAG 1 cut(s) 139
BpuEI CTTGAG 1 cut(s) 1415
BpuMI CCSGG 3 cut(s) 230, 458, 1149
BsaBI GATNNNNATC 1 cut(s) 1214
BsaJI CCNNGG 6 cut(s) 228, 243, 457, 546, 1148, 1310
BsaWI WCCGGW 3 cut(s) 105, 508, 608
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 350, 380
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 887
Bse1I ACTGG 1 cut(s) 1453
Bse3DI GCAATG 1 cut(s) 970
Bse8I GATNNNNATC 1 cut(s) 1214
BseBI CCWGG 4 cut(s) 157, 245, 583, 1014
BseDI CCNNGG 6 cut(s) 228, 243, 457, 546, 1148, 1310
BseGI GGATG 5 cut(s) 21, 42, 84, 1396, 1450
BseJI GATNNNNATC 1 cut(s) 1214
BseLI CCNNNNNNNGG 2 cut(s) 229, 887
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 3 cut(s) 202, 633, 1105
BseNI ACTGG 1 cut(s) 1453
BseRI GAGGAG 4 cut(s) 176, 208, 415, 844
BseXI GCAGC 8 cut(s) 137, 280, 348, 565, 655, 672, 841, 903
BseYI CCCAGC 1 cut(s) 165
Bsh1236I CGCG 2 cut(s) 315, 575
Bsh1285I CGRYCG 2 cut(s) 288, 437
BshFI GGCC 1 cut(s) 599
BsiEI CGRYCG 2 cut(s) 288, 437
BsiHKAI GWGCWC 2 cut(s) 491, 540
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 6 cut(s) 106, 230, 457, 509, 609, 1148
BsiWI CGTACG 1 cut(s) 1242
BslFI GGGAC 3 cut(s) 253, 431, 920
BslI CCNNNNNNNGG 2 cut(s) 229, 887
BsmAI GTCTC 3 cut(s) 118, 157, 973
BsmBI CGTCTC 2 cut(s) 118, 157
BsmFI GGGAC 3 cut(s) 253, 431, 920
BsnI GGCC 1 cut(s) 599
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 491, 540
Bsp143I GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
Bsp19I CCATGG 1 cut(s) 546
BspANI GGCC 1 cut(s) 599
BspCNI CTCAG 3 cut(s) 201, 634, 1104
BspFNI CGCG 2 cut(s) 315, 575
BspLI GGNNCC 3 cut(s) 327, 908, 1249
BspMAI CTGCAG 1 cut(s) 859
BspPI GGATC 1 cut(s) 872
BspQI GCTCTTC 1 cut(s) 545
BsrBI CCGCTC 2 cut(s) 259, 619
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 1 cut(s) 1453
BssECI CCNNGG 6 cut(s) 228, 243, 457, 546, 1148, 1310
BssMI GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
BssSI CACGAG 1 cut(s) 533
BssT1I CCWWGG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 533
Bst2UI CCWGG 4 cut(s) 157, 245, 583, 1014
Bst4CI ACNGT 6 cut(s) 73, 277, 286, 435, 1100, 1314
Bst6I CTCTTC 2 cut(s) 545, 651
BstAPI GCANNNNNTGC 1 cut(s) 668
BstC8I GCNNGC 2 cut(s) 1107, 1133
BstDEI CTNAG 3 cut(s) 188, 642, 1091
BstDSI CCRYGG 2 cut(s) 546, 1310
BstF5I GGATG 5 cut(s) 21, 42, 84, 1396, 1450
BstFNI CGCG 2 cut(s) 315, 575
BstHHI GCGC 2 cut(s) 315, 577
BstKTI GATC 6 cut(s) 468, 594, 769, 880, 1080, 1212
BstMAI GTCTC 3 cut(s) 118, 157, 973
BstMBI GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
BstMCI CGRYCG 2 cut(s) 288, 437
BstMWI GCNNNNNNNGC 6 cut(s) 299, 559, 574, 668, 851, 1327
BstNI CCWGG 4 cut(s) 157, 245, 583, 1014
BstSCI CCNGG 7 cut(s) 155, 228, 243, 456, 581, 1012, 1147
BstSFI CTRYAG 1 cut(s) 855
BstUI CGCG 2 cut(s) 315, 575
BstV1I GCAGC 8 cut(s) 137, 280, 348, 565, 655, 672, 841, 903
BstV2I GAAGAC 1 cut(s) 351
BstXI CCANNNNNNTGG 1 cut(s) 917
BsuRI GGCC 1 cut(s) 599
BtgI CCRYGG 2 cut(s) 546, 1310
BtsCI GGATG 5 cut(s) 21, 42, 84, 1396, 1450
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 3 cut(s) 724, 1099, 1105
Cac8I GCNNGC 2 cut(s) 1107, 1133
CfoI GCGC 2 cut(s) 315, 577
Cfr13I GGNCC 5 cut(s) 232, 451, 523, 788, 907
CseI GACGC 3 cut(s) 100, 304, 358
CsiI ACCWGGT 1 cut(s) 581
Csp6I GTAC 4 cut(s) 431, 478, 1243, 1273
CviAII CATG 6 cut(s) 16, 310, 463, 547, 734, 842
CviQI GTAC 4 cut(s) 431, 478, 1243, 1273
DdeI CTNAG 3 cut(s) 188, 642, 1091
DpnI GATC 6 cut(s) 467, 593, 768, 879, 1079, 1211
DpnII GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
Eam1104I CTCTTC 2 cut(s) 545, 651
EarI CTCTTC 2 cut(s) 545, 651
EciI GGCGGA 2 cut(s) 17, 77
Ecl136II GAGCTC 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 491
Eco32I GATATC 1 cut(s) 1181
Eco47I GGWCC 5 cut(s) 232, 451, 523, 788, 907
Eco53kI GAGCTC 1 cut(s) 489
Eco57I CTGAAG 2 cut(s) 407, 1343
Eco88I CYCGRG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 489
EcoRI GAATTC 1 cut(s) 612
EcoRII CCWGG 4 cut(s) 155, 243, 581, 1012
EcoRV GATATC 1 cut(s) 1181
EcoT14I CCWWGG 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 546
Esp3I CGTCTC 2 cut(s) 118, 157
FaeI CATG 6 cut(s) 19, 313, 466, 550, 737, 845
FaqI GGGAC 3 cut(s) 253, 431, 920
FatI CATG 6 cut(s) 15, 309, 462, 546, 733, 841
FauI CCCGC 2 cut(s) 264, 1273
FokI GGATG 4 cut(s) 8, 29, 71, 1403
FriOI GRGCYC 1 cut(s) 491
FspBI CTAG 4 cut(s) 810, 1029, 1058, 1406
GlaI GCGC 2 cut(s) 314, 576
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 139
HaeIII GGCC 1 cut(s) 599
HapII CCGG 6 cut(s) 106, 230, 457, 509, 609, 1148
HgaI GACGC 3 cut(s) 100, 304, 358
HhaI GCGC 2 cut(s) 315, 577
Hin1II CATG 6 cut(s) 19, 313, 466, 550, 737, 845
Hin6I GCGC 2 cut(s) 313, 575
HinP1I GCGC 2 cut(s) 313, 575
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 6 cut(s) 266, 496, 602, 725, 743, 1215
HpaII CCGG 6 cut(s) 106, 230, 457, 509, 609, 1148
HphI GGTGA 4 cut(s) 571, 1036, 1154, 1274
Hpy166II GTNNAC 5 cut(s) 451, 478, 523, 606, 652
Hpy188I TCNGA 8 cut(s) 174, 191, 493, 643, 816, 850, 1214, 1220
Hpy188III TCNNGA 6 cut(s) 373, 469, 500, 589, 1081, 1202
Hpy8I GTNNAC 5 cut(s) 451, 478, 523, 606, 652
Hpy99I CGWCG 4 cut(s) 77, 122, 281, 374
HpyAV CCTTC 3 cut(s) 214, 370, 854
HpyCH4III ACNGT 6 cut(s) 73, 277, 286, 435, 1100, 1314
HpyCH4IV ACGT 2 cut(s) 150, 1241
HpyCH4V TGCA 5 cut(s) 717, 752, 857, 1109, 1135
HpyF10VI GCNNNNNNNGC 6 cut(s) 299, 559, 574, 668, 851, 1327
HpyF3I CTNAG 3 cut(s) 188, 642, 1091
HpySE526I ACGT 2 cut(s) 150, 1241
Hsp92II CATG 6 cut(s) 19, 313, 466, 550, 737, 845
HspAI GCGC 2 cut(s) 313, 575
Kzo9I GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
LguI GCTCTTC 1 cut(s) 545
LmnI GCTCC 8 cut(s) 189, 325, 358, 486, 494, 550, 831, 1318
Lsp1109I GCAGC 8 cut(s) 137, 280, 348, 565, 655, 672, 841, 903
LweI GCATC 5 cut(s) 348, 841, 1077, 1144, 1411
MabI ACCWGGT 1 cut(s) 581
MaeI CTAG 4 cut(s) 810, 1029, 1058, 1406
MaeII ACGT 2 cut(s) 150, 1241
MaeIII GTNAC 2 cut(s) 1042, 1094
MalI GATC 6 cut(s) 467, 593, 768, 879, 1079, 1211
MbiI CCGCTC 2 cut(s) 259, 619
MboI GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
MboII GAAGA 6 cut(s) 356, 400, 532, 668, 1096, 1336
MhlI GDGCHC 2 cut(s) 491, 540
MluCI AATT 6 cut(s) 6, 393, 612, 882, 1226, 1376
MlyI GAGTC 2 cut(s) 275, 611
MmeI TCCRAC 3 cut(s) 898, 1219, 1296
MseI TTAA 3 cut(s) 396, 819, 1034
MslI CAYNNNNRTG 1 cut(s) 1140
MspA1I CMGCKG 2 cut(s) 293, 484
MspI CCGG 6 cut(s) 106, 230, 457, 509, 609, 1148
MspR9I CCNGG 7 cut(s) 157, 230, 245, 458, 583, 1014, 1149
MvaI CCWGG 4 cut(s) 157, 245, 583, 1014
MvnI CGCG 2 cut(s) 315, 575
MwoI GCNNNNNNNGC 6 cut(s) 299, 559, 574, 668, 851, 1327
NciI CCSGG 3 cut(s) 230, 458, 1149
NcoI CCATGG 1 cut(s) 546
NdeII GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
NlaIII CATG 6 cut(s) 19, 313, 466, 550, 737, 845
NlaIV GGNNCC 3 cut(s) 327, 908, 1249
NmeAIII GCCGAG 1 cut(s) 625
NmuCI GTSAC 2 cut(s) 1042, 1094
PaeR7I CTCGAG 1 cut(s) 563
PciSI GCTCTTC 1 cut(s) 545
PfeI GAWTC 4 cut(s) 496, 725, 743, 1215
Pfl23II CGTACG 1 cut(s) 1242
PfoI TCCNGGA 1 cut(s) 155
PleI GAGTC 2 cut(s) 274, 610
PpsI GAGTC 2 cut(s) 274, 610
PshBI ATTAAT 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 491
Psp6I CCWGG 4 cut(s) 155, 243, 581, 1012
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 4 cut(s) 155, 243, 581, 1012
PspLI CGTACG 1 cut(s) 1242
PspN4I GGNNCC 3 cut(s) 327, 908, 1249
PspPI GGNCC 5 cut(s) 232, 451, 523, 788, 907
PspXI VCTCGAGB 1 cut(s) 563
PstI CTGCAG 1 cut(s) 859
RsaI GTAC 4 cut(s) 432, 479, 1244, 1274
RsaNI GTAC 4 cut(s) 431, 478, 1243, 1273
RseI CAYNNNNRTG 1 cut(s) 1140
SacI GAGCTC 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 545
SaqAI TTAA 3 cut(s) 396, 819, 1034
Sau3AI GATC 6 cut(s) 465, 591, 766, 877, 1077, 1209
Sau96I GGNCC 5 cut(s) 232, 451, 523, 788, 907
SchI GAGTC 2 cut(s) 275, 611
ScrFI CCNGG 7 cut(s) 157, 230, 245, 458, 583, 1014, 1149
SduI GDGCHC 2 cut(s) 491, 540
SexAI ACCWGGT 1 cut(s) 581
SfaNI GCATC 5 cut(s) 348, 841, 1077, 1144, 1411
SfcI CTRYAG 1 cut(s) 855
Sfr274I CTCGAG 1 cut(s) 563
SinI GGWCC 5 cut(s) 232, 451, 523, 788, 907
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 1140
SmlI CTYRAG 2 cut(s) 563, 1430
SmoI CTYRAG 2 cut(s) 563, 1430
Sse9I AATT 6 cut(s) 6, 393, 612, 882, 1226, 1376
SspMI CTAG 4 cut(s) 810, 1029, 1058, 1406
SstI GAGCTC 1 cut(s) 491
StyD4I CCNGG 7 cut(s) 155, 228, 243, 456, 581, 1012, 1147
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 6 cut(s) 73, 277, 286, 435, 1100, 1314
TaiI ACGT 2 cut(s) 153, 1244
TaqI TCGA 7 cut(s) 342, 437, 468, 564, 594, 728, 1371
TaqII GACCGA 1 cut(s) 776
TasI AATT 6 cut(s) 6, 393, 612, 882, 1226, 1376
TauI GCSGC 4 cut(s) 59, 62, 125, 293
TfiI GAWTC 4 cut(s) 496, 725, 743, 1215
Tru1I TTAA 3 cut(s) 396, 819, 1034
Tru9I TTAA 3 cut(s) 396, 819, 1034
TscAI CASTG 3 cut(s) 724, 1099, 1105
TseFI GTSAC 2 cut(s) 1042, 1094
TseI GCWGC 8 cut(s) 125, 293, 361, 553, 668, 685, 854, 891
Tsp45I GTSAC 2 cut(s) 1042, 1094
TspDTI ATGAA 3 cut(s) 298, 1370, 1389
TspGWI ACGGA 2 cut(s) 106, 1260
TspRI CASTG 3 cut(s) 724, 1099, 1105
VpaK11BI GGWCC 5 cut(s) 232, 451, 523, 788, 907
VspI ATTAAT 1 cut(s) 819
XapI RAATTY 2 cut(s) 612, 1226
XhoI CTCGAG 1 cut(s) 563
XspI CTAG 4 cut(s) 810, 1029, 1058, 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.