Rh4DG304900

ribosome biogenesis GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010219
Physical Location & Seq
Reverse (-)
52715051 .. 52718072
3022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG304900.1

Sequence Viewer

Length: 1431 bp
ATGCCGACTGCCTCCATCTCAATTCTCCGCCACCGCTCCGTCACCGTCACTCTCCCCACCTTCAACGACCTCTGCCGCCGATTCTCAACCGTCCGATGCGTCCCTATCGCAGCAGCAAAGCACCCTAACGTCACCAGAAAATCGCAGCCGCCGGACAAGAAGCTCCTCCGAGCTAAACAGACCTTCAAGGACTACTCCTCCCTCGCCCCGGTCCTCTCTCCTCAGGACAAGCCTCCTCTCTCGGAATCCCAGACCGTCGGCACCGTCGCCGCCGCTCAGGCCAACTTCATGCGCGTCATCGTCGAACCTCCTGGAAGCCTCGAGAAAAGCGCGGACGAAACGACGTCGTCGTCTTCTGGATTTCAGGTGGAGCTGCTGTGTGTGGTGAGGGCGTTGTTGAAGAAGATAAAGAGGAGAGTGTTGGTTGGGGACAAGGTGCTGGTGGGGTCCATTGACTGGGTGGACCGCCGGGGAATGATCGAGAATGTGTACCGGAGGAGCTCCGAGATTCTTGACCCGCCGGTGGCCAATGTGGACCATCTGCTCGTGCTTTTCTCTCTGGAGCAGCCCAAGCTTGACCCCTTCGCGCTCACTAGGTTTCTGGTTGAGGCCGAGTCCACTGCAATTCCACTCACTCTGGCTCTCAACAAGTCTGAGCTGGTTGACGAAGAGACAAAGGCTATATGGAAGTCTAGGCTACATAAGTGGGGCTACAAGCCACTATTTTGCAGCGTTGAATCTAAATTTGGACTCGACTCTCTTGCTTTTATTTTGAGAGATCAAACAAGTGTAATTGTGGGTCCAAGTGGAGTTGGAAAGTCTAGTCTGATCAATGCTCTGAGGAACAGCGACCATCATGCTTTTGATGCTGCAGAAGTGGATAATCGGCTTGATCCAATTTCAGGCAGCAAGTGGTTGGAGGATCAGCGTGTTGGAGAGGTTTCAGTAAGAAGTGGTAAAGGGAAGCATACCACTCGCCACGTTTCCTTGCTTCCATTGTCTGGAGGAGGGTATCTTGCTGATACTCCTGGGTTCAGCCAGCCTAGTTTAATGAAAGTAACAAAGCAATCTCTGGCACAAGCTTTCCCCGAGATACAGAAGATACTCAATGACAGTGAAGCTGCGAAATGCTCATTCAGTGACTGCTTGCATTTGGGTGAAAAAGGTTGCGTTGTAGGGACTGACTGGGAAAGATACCCGTACTATTTCCAACTTCTTGATGAGATCAGAATCAGAGAGGAATTTCAGTTAAGGACATTTGGAACCAAAAAAGAGGGTGATGTAAGGATCAAGGATGGACAAGCTGAACCAAGGTTGGAGCGTAAGAAGCATAGGAGAGTATCTCGCAAGAGTATGAACCAATCAGTACTAGATGAATTAGATGAGCTTGATGACGACGACGACGACTTACTAGATGAGGTGAACCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
RsgA_GTPase

Protein Analysis

476

Amino Acids

53.18

Weight (kDa)

8.33

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsgA_GTPase PF03193 164 - 350 3.2e-46 RsgA GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 349
AatII GACGTC 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 2 cut(s) 456, 1001
AccBSI CCGCTC 2 cut(s) 36, 275
AccII CGCG 3 cut(s) 294, 332, 587
AciI CCGC 9 cut(s) 28, 34, 76, 149, 270, 273, 332, 466, 518
AclWI GGATC 3 cut(s) 887, 930, 1295
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 743, 1241
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 3 cut(s) 491, 1202, 1368
AfiI CCNNNNNNNGG 5 cut(s) 208, 456, 523, 902, 1001
AgsI TTSAA 4 cut(s) 64, 187, 400, 737
AjnI CCWGG 2 cut(s) 310, 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 729, 761
Alw21I GWGCWC 1 cut(s) 503
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 3 cut(s) 887, 930, 1295
AlwNI CAGNNNCTG 1 cut(s) 1143
Ama87I CYCGRG 2 cut(s) 320, 1088
AoxI GGCC 3 cut(s) 279, 525, 609
ApeKI GCWGC 9 cut(s) 110, 113, 145, 373, 565, 729, 869, 906, 1121
ApoI RAATTY 2 cut(s) 743, 1241
AspLEI GCGC 3 cut(s) 294, 332, 589
AspS9I GGNCC 5 cut(s) 211, 447, 463, 535, 800
AsuC2I CCSGG 2 cut(s) 209, 470
AsuHPI GGTGA 5 cut(s) 34, 124, 397, 1169, 1289
AvaI CYCGRG 2 cut(s) 320, 1088
AvaII GGWCC 5 cut(s) 211, 447, 463, 535, 800
AxyI CCTNAGG 1 cut(s) 222
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 503
BauI CACGAG 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 9 cut(s) 122, 125, 157, 360, 577, 741, 856, 918, 1108
BccI CCATC 4 cut(s) 23, 546, 861, 1289
BcgI CGANNNNNNTGC 8 cut(s) 602, 636, 743, 777, 839, 873, 956, 990
BciT130I CCWGG 2 cut(s) 312, 1029
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 2 cut(s) 209, 470
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 6 cut(s) 594, 693, 822, 1044, 1370, 1412
BfmI CTRYAG 1 cut(s) 870
BglI GCCNNNNNGGC 1 cut(s) 278
BmcAI AGTACT 1 cut(s) 1368
Bme1390I CCNGG 4 cut(s) 209, 312, 470, 1029
Bme18I GGWCC 5 cut(s) 211, 447, 463, 535, 800
BmeT110I CYCGRG 2 cut(s) 320, 1088
BmgT120I GGNCC 5 cut(s) 211, 447, 463, 535, 800
BmiI GGNNCC 4 cut(s) 262, 448, 801, 1264
BmrFI CCNGG 4 cut(s) 209, 312, 470, 1029
BmrI ACTGGG 2 cut(s) 466, 1195
BmsI GCATC 2 cut(s) 86, 856
BmuI ACTGGG 2 cut(s) 466, 1195
BpiI GAAGAC 1 cut(s) 345
BplI GAGNNNNNCTC 2 cut(s) 1327, 1359
BpmI CTGGAG 2 cut(s) 581, 1023
Bpu10I CCTNAGC 1 cut(s) 276
BpuMI CCSGG 2 cut(s) 209, 470
BsaBI GATNNNNATC 1 cut(s) 1229
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 4 cut(s) 207, 469, 1028, 1310
BsaWI WCCGGW 1 cut(s) 492
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 362, 392
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 456, 523, 902, 1001
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 2 cut(s) 461, 1190
Bse21I CCTNAGG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 1229
BseBI CCWGG 2 cut(s) 312, 1029
BseDI CCNNGG 4 cut(s) 207, 469, 1028, 1310
BseGI GGATG 1 cut(s) 1300
BseJI GATNNNNATC 1 cut(s) 1229
BseLI CCNNNNNNNGG 5 cut(s) 208, 456, 523, 902, 1001
BseMII CTCAG 4 cut(s) 236, 290, 645, 830
BseNI ACTGG 2 cut(s) 461, 1190
BseRI GAGGAG 7 cut(s) 155, 187, 210, 225, 427, 511, 1020
BseXI GCAGC 9 cut(s) 122, 125, 157, 360, 577, 741, 856, 918, 1108
Bsh1236I CGCG 3 cut(s) 294, 332, 587
BshFI GGCC 3 cut(s) 281, 527, 611
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 503
BsiHKCI CYCGRG 2 cut(s) 320, 1088
BsiSI CCGG 5 cut(s) 152, 209, 469, 493, 521
BslFI GGGAC 3 cut(s) 86, 443, 1192
BslI CCNNNNNNNGG 5 cut(s) 208, 456, 523, 902, 1001
BsmAI GTCTC 1 cut(s) 665
BsmFI GGGAC 3 cut(s) 86, 443, 1192
BsnI GGCC 3 cut(s) 281, 527, 611
BsoBI CYCGRG 2 cut(s) 320, 1088
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
BspACI CCGC 9 cut(s) 28, 34, 76, 149, 270, 273, 332, 466, 518
BspANI GGCC 3 cut(s) 281, 527, 611
BspCNI CTCAG 4 cut(s) 235, 289, 646, 831
BspFNI CGCG 3 cut(s) 294, 332, 587
BspLI GGNNCC 4 cut(s) 262, 448, 801, 1264
BspMAI CTGCAG 1 cut(s) 874
BspPI GGATC 3 cut(s) 887, 930, 1295
BspT107I GGYRCC 1 cut(s) 260
BsrBI CCGCTC 2 cut(s) 36, 275
BsrFI RCCGGY 1 cut(s) 520
BsrI ACTGG 2 cut(s) 461, 1190
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 4 cut(s) 207, 469, 1028, 1310
BssMI GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
BssNI GRCGYC 1 cut(s) 344
BssSI CACGAG 1 cut(s) 545
BssT1I CCWWGG 1 cut(s) 1310
Bst2BI CACGAG 1 cut(s) 545
Bst2UI CCWGG 2 cut(s) 312, 1029
Bst4CI ACNGT 5 cut(s) 46, 91, 256, 265, 1115
Bst6I CTCTTC 1 cut(s) 663
BstACI GRCGYC 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 1040, 1148
BstDEI CTNAG 4 cut(s) 222, 276, 654, 839
BstF5I GGATG 1 cut(s) 1300
BstFNI CGCG 3 cut(s) 294, 332, 587
BstHHI GCGC 3 cut(s) 294, 332, 589
BstKTI GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
BstMWI GCNNNNNNNGC 4 cut(s) 278, 571, 866, 1327
BstNI CCWGG 2 cut(s) 312, 1029
BstSCI CCNGG 4 cut(s) 207, 310, 468, 1027
BstSFI CTRYAG 1 cut(s) 870
BstUI CGCG 3 cut(s) 294, 332, 587
BstV1I GCAGC 9 cut(s) 122, 125, 157, 360, 577, 741, 856, 918, 1108
BstV2I GAAGAC 1 cut(s) 345
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 3 cut(s) 281, 527, 611
BtsCI GGATG 1 cut(s) 1300
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 3 cut(s) 618, 1120, 1144
Cac8I GCNNGC 2 cut(s) 1040, 1148
CaiI CAGNNNCTG 1 cut(s) 1143
CfoI GCGC 3 cut(s) 294, 332, 589
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 5 cut(s) 211, 447, 463, 535, 800
CseI GACGC 2 cut(s) 88, 283
Csp6I GTAC 3 cut(s) 490, 1201, 1367
CviAII CATG 2 cut(s) 289, 857
CviQI GTAC 3 cut(s) 490, 1201, 1367
DdeI CTNAG 4 cut(s) 222, 276, 654, 839
DpnI GATC 7 cut(s) 479, 780, 830, 894, 924, 1226, 1289
DpnII GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
DrdI GACNNNNNNGTC 1 cut(s) 349
DseDI GACNNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
EciI GGCGGA 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 1310
Eco24I GRGCYC 1 cut(s) 503
Eco47I GGWCC 5 cut(s) 211, 447, 463, 535, 800
Eco53kI GAGCTC 1 cut(s) 501
Eco81I CCTNAGG 1 cut(s) 222
Eco88I CYCGRG 2 cut(s) 320, 1088
EcoICRI GAGCTC 1 cut(s) 501
EcoRII CCWGG 2 cut(s) 310, 1027
EcoT14I CCWWGG 1 cut(s) 1310
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 1 cut(s) 1310
FaeI CATG 2 cut(s) 292, 860
FaiI YATR 8 cut(s) 290, 683, 685, 702, 858, 969, 1332, 1355
FaqI GGGAC 3 cut(s) 86, 443, 1192
FatI CATG 2 cut(s) 288, 856
FauI CCCGC 1 cut(s) 525
FbaI TGATCA 1 cut(s) 828
FokI GGATG 1 cut(s) 1307
FriOI GRGCYC 1 cut(s) 503
FspBI CTAG 6 cut(s) 594, 693, 822, 1044, 1370, 1412
GlaI GCGC 3 cut(s) 293, 331, 588
GsuI CTGGAG 2 cut(s) 581, 1023
HaeIII GGCC 3 cut(s) 281, 527, 611
HapII CCGG 5 cut(s) 152, 209, 469, 493, 521
HgaI GACGC 2 cut(s) 88, 283
HhaI GCGC 3 cut(s) 294, 332, 589
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 2 cut(s) 292, 860
Hin6I GCGC 3 cut(s) 292, 330, 587
HinP1I GCGC 3 cut(s) 292, 330, 587
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 2 cut(s) 572, 1080
HinfI GANTC 8 cut(s) 81, 245, 508, 614, 737, 750, 755, 1230
HpaII CCGG 5 cut(s) 152, 209, 469, 493, 521
HphI GGTGA 5 cut(s) 34, 124, 397, 1169, 1289
Hpy166II GTNNAC 6 cut(s) 463, 490, 535, 618, 664, 1423
Hpy188I TCNGA 9 cut(s) 95, 170, 244, 505, 655, 828, 840, 1229, 1235
Hpy188III TCNNGA 8 cut(s) 224, 322, 357, 481, 512, 560, 1002, 1217
Hpy8I GTNNAC 6 cut(s) 463, 490, 535, 618, 664, 1423
Hpy99I CGWCG 9 cut(s) 260, 269, 305, 346, 349, 352, 1400, 1403, 1406
HpyAV CCTTC 3 cut(s) 70, 193, 592
HpyCH4III ACNGT 5 cut(s) 46, 91, 256, 265, 1115
HpyCH4IV ACGT 3 cut(s) 129, 344, 981
HpyCH4V TGCA 4 cut(s) 623, 729, 872, 1150
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 571, 866, 1327
HpyF3I CTNAG 4 cut(s) 222, 276, 654, 839
HpySE526I ACGT 3 cut(s) 129, 344, 981
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 2 cut(s) 292, 860
HspAI GCGC 3 cut(s) 292, 330, 587
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
LmnI GCTCC 7 cut(s) 41, 168, 370, 498, 506, 562, 1318
Lsp1109I GCAGC 9 cut(s) 122, 125, 157, 360, 577, 741, 856, 918, 1108
LweI GCATC 2 cut(s) 86, 856
MaeI CTAG 6 cut(s) 594, 693, 822, 1044, 1370, 1412
MaeII ACGT 3 cut(s) 129, 344, 981
MaeIII GTNAC 5 cut(s) 40, 46, 130, 1057, 1139
MalI GATC 7 cut(s) 479, 780, 830, 894, 924, 1226, 1289
MbiI CCGCTC 2 cut(s) 36, 275
MboI GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
MboII GAAGA 5 cut(s) 345, 412, 415, 680, 1111
MhlI GDGCHC 1 cut(s) 503
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 7 cut(s) 21, 624, 743, 792, 897, 1241, 1376
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 3 cut(s) 623, 744, 749
MmeI TCCRAC 5 cut(s) 793, 897, 913, 1234, 1296
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 2 cut(s) 1049, 1250
MslI CAYNNNNRTG 1 cut(s) 1155
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 5 cut(s) 152, 209, 469, 493, 521
MspR9I CCNGG 4 cut(s) 209, 312, 470, 1029
MvaI CCWGG 2 cut(s) 312, 1029
MvnI CGCG 3 cut(s) 294, 332, 587
MwoI GCNNNNNNNGC 4 cut(s) 278, 571, 866, 1327
NciI CCSGG 2 cut(s) 209, 470
NdeII GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
NlaIII CATG 2 cut(s) 292, 860
NlaIV GGNNCC 4 cut(s) 262, 448, 801, 1264
NmeAIII GCCGAG 1 cut(s) 637
NmuCI GTSAC 4 cut(s) 40, 46, 130, 1139
PaeR7I CTCGAG 1 cut(s) 320
PcsI WCGNNNNNNNCGW 2 cut(s) 347, 1401
PfeI GAWTC 5 cut(s) 81, 245, 508, 737, 1230
PflFI GACNNNGTC 1 cut(s) 346
PflMI CCANNNNNTGG 2 cut(s) 456, 1001
PfoI TCCNGGA 1 cut(s) 310
PleI GAGTC 3 cut(s) 622, 744, 749
PpsI GAGTC 3 cut(s) 622, 744, 749
Psp124BI GAGCTC 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 310, 1027
PspGI CCWGG 2 cut(s) 310, 1027
PspN4I GGNNCC 4 cut(s) 262, 448, 801, 1264
PspPI GGNCC 5 cut(s) 211, 447, 463, 535, 800
PstI CTGCAG 1 cut(s) 874
PstNI CAGNNNCTG 1 cut(s) 1143
PsyI GACNNNGTC 1 cut(s) 346
RsaI GTAC 3 cut(s) 491, 1202, 1368
RsaNI GTAC 3 cut(s) 490, 1201, 1367
RseI CAYNNNNRTG 1 cut(s) 1155
SacI GAGCTC 1 cut(s) 503
SaqAI TTAA 2 cut(s) 1049, 1250
Sau3AI GATC 7 cut(s) 477, 778, 828, 892, 922, 1224, 1287
Sau96I GGNCC 5 cut(s) 211, 447, 463, 535, 800
ScaI AGTACT 1 cut(s) 1368
SchI GAGTC 3 cut(s) 623, 744, 749
ScrFI CCNGG 4 cut(s) 209, 312, 470, 1029
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 2 cut(s) 86, 856
SfcI CTRYAG 1 cut(s) 870
Sfr274I CTCGAG 1 cut(s) 320
SgrAI CRCCGGYG 1 cut(s) 520
SinI GGWCC 5 cut(s) 211, 447, 463, 535, 800
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 1155
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 7 cut(s) 21, 624, 743, 792, 897, 1241, 1376
SsiI CCGC 9 cut(s) 28, 34, 76, 149, 270, 273, 332, 466, 518
SspMI CTAG 6 cut(s) 594, 693, 822, 1044, 1370, 1412
SstI GAGCTC 1 cut(s) 503
StyD4I CCNGG 4 cut(s) 207, 310, 468, 1027
StyI CCWWGG 1 cut(s) 1310
TaaI ACNGT 5 cut(s) 46, 91, 256, 265, 1115
TaiI ACGT 3 cut(s) 132, 347, 984
TaqI TCGA 4 cut(s) 303, 321, 480, 753
TasI AATT 7 cut(s) 21, 624, 743, 792, 897, 1241, 1376
TatI WGTACW 1 cut(s) 1366
TauI GCSGC 4 cut(s) 78, 151, 272, 275
TfiI GAWTC 5 cut(s) 81, 245, 508, 737, 1230
Tru1I TTAA 2 cut(s) 1049, 1250
Tru9I TTAA 2 cut(s) 1049, 1250
TscAI CASTG 3 cut(s) 625, 1120, 1144
TseFI GTSAC 4 cut(s) 40, 46, 130, 1139
TseI GCWGC 9 cut(s) 110, 113, 145, 373, 565, 729, 869, 906, 1121
Tsp45I GTSAC 4 cut(s) 40, 46, 130, 1139
TspDTI ATGAA 4 cut(s) 277, 1067, 1370, 1389
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 3 cut(s) 625, 1120, 1144
Tth111I GACNNNGTC 1 cut(s) 346
Van91I CCANNNNNTGG 2 cut(s) 456, 1001
VpaK11BI GGWCC 5 cut(s) 211, 447, 463, 535, 800
XapI RAATTY 2 cut(s) 743, 1241
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 6 cut(s) 594, 693, 822, 1044, 1370, 1412
ZraI GACGTC 1 cut(s) 345
ZrmI AGTACT 1 cut(s) 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.